公开(公告)号：US20150119298A1

公开(公告)日：2015-04-30

申请号：US14500399

申请日：2014-09-29

Applicant: Fluidigm Corporation

Inventor： Gang Sun ,  Greg Harris ,  Andy May ,  Kyle Self ,  Kevin Farrell ,  Paul Wyatt

IPC: G01N35/00

CPC classification number: G01N35/00029 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2200/10 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0829 ,  C12Q1/686 ,  G01N15/1475 ,  G01N21/6428 ,  G01N35/00732 ,  G01N35/00871 ,  G01N35/0092 ,  G01N35/0099 ,  G01N35/1065 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/00752 ,  G01N2035/0091 ,  G01N2035/1034 ,  G01N2201/025 ,  G01N2201/0446 ,  Y10S901/01 ,  Y10T436/11

Abstract: A system for performing one or more microfluidic processes includes an integrated fluidic device comprising a plurality of well regions and a plurality of control valves and a workflow manager. The system also includes a transfer robot adapted to transfer the integrated fluidic device between a plurality of stations in response to a series of instructions from the workflow manager and a first station comprising a dispensing robot adapted to dispense at least one of a plurality of sample solutions and at least one of a plurality of reagents into the integrated fluidic device. The system further includes a second station comprising a fluidic controller unit and a third station comprising an inspection station.

Abstract translation: 用于执行一个或多个微流程过程的系统包括包括多个井区域和多个控制阀以及工作流程管理器的集成流体设备。 该系统还包括传送机器人，其适于响应于来自工作流程管理器的一系列指令而在多个站之间传送集成流体设备，第一站包括分配机器人，其适于分配多个样品溶液中的至少一个样品溶液 和多个试剂中的至少一种进入集成流体装置。 该系统还包括第二站，其包括流体控制器单元和包括检查站的第三站。

公开(公告)号：US20140154787A1

公开(公告)日：2014-06-05

申请号：US13915476

申请日：2013-06-11

Applicant: Fluidigm Corporation

Inventor： Gang Sun ,  Ramesh Ramakrishnan ,  Robert C. Jones

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q2565/629 ,  C12Q2565/501 ,  C12Q2545/114

Abstract: A method of adjusting amplification curves in a PCR experiment includes receiving a plurality of amplification curves for a sample and computing a first parameter for each of the plurality of amplification curves. The method also includes computing a second parameter for each of the plurality of amplification curves and computing a third parameter using at least a portion of the first or second parameters. The method further includes computing an offset for each of the plurality of amplification curves. The offset is a function of the first parameter and the third parameter. Moreover, the method includes adjusting at least one of the plurality of amplification curves by subtracting the offset.

Abstract translation: 在PCR实验中调整扩增曲线的方法包括接收样品的多个放大曲线并计算多个放大曲线中的每一个的第一参数。 该方法还包括为多个放大曲线中的每一个计算第二参数，并使用第一或第二参数的至少一部分来计算第三参数。 该方法还包括计算多个放大曲线中的每一个的偏移量。 偏移量是第一个参数和第三个参数的函数。 此外，该方法包括通过减去偏移来调整多个放大曲线中的至少一个。

公开(公告)号：US20140133732A1

公开(公告)日：2014-05-15

申请号：US14071423

申请日：2013-11-04

Applicant: Fluidigm Corporation

Inventor： Simant Dube ,  Gang Sun ,  Lian-She Zhao

IPC: G01N21/64

CPC classification number: G01N21/64 ,  G06T7/0012 ,  G06T7/74 ,  G06T2207/10064 ,  G06T2207/30004

Abstract: A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.

Abstract translation: 处理与微流体装置的荧光发射有关的数据的方法。 该方法包括执行与微流体装置的第一图像相关联的自动聚焦处理，并且执行与微流体装置的第一图像相关联的自动曝光处理。 该方法还包括捕获微流体装置的多个图像。 多个图像与多个热循环相关联。 该方法还包括执行多个拍摄图像的图像分析以确定一系列光强度并执行该系列光强度的数据分析以提供一系列阈值变化。