公开(公告)号：US09399796B2

公开(公告)日：2016-07-26

申请号：US13956158

申请日：2013-07-31

Applicant: Gen-Probe Incorporated

Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  Norman C. Nelson ,  James D. Carlson ,  Michael M. Becker ,  Lyle J. Arnold, Jr.

IPC: C12Q1/68

CPC classification number: C12Q1/6865 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2549/119 ,  C12Q2537/143 ,  C12Q2531/143

Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

公开(公告)号：US20200248246A1

公开(公告)日：2020-08-06

申请号：US16849857

申请日：2020-04-15

Applicant: GEN-PROBE INCORPORATED

Inventor： James Carlson ,  Reinhold Pollner ,  Steven T. Brentano

IPC: C12Q1/6834 ,  C12Q1/6816

Abstract: The invention provides an improved stem-loop target capture oligomer and methods of use. Such a target capture oligomer has a target-binding segment forming a loop flanked by stem segments forming a stem. The stem segments are of unequal length. Such probes show little or no binding to immobilized probes in the absence of a target nucleic acid but offer good target sensitivity. The probes are particularly useful in multiplex methods of detection in which multiple target capture oligomers are present for detecting of multiple target nucleic acids (for example, detecting multiple polymorphic forms of a target gene).

公开(公告)号：US10724085B2

公开(公告)日：2020-07-28

申请号：US15902819

申请日：2018-02-22

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  Norman C. Nelson ,  James D. Carlson ,  Michael M. Becker ,  Lyle J. Arnold, Jr.

IPC: C12Q1/68 ,  C12Q1/6865 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6853

Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

公开(公告)号：US10392656B2

公开(公告)日：2019-08-27

申请号：US15277167

申请日：2016-09-27

Applicant: GEN-PROBE INCORPORATED

Inventor： James D. Carlson ,  Steven T. Brentano

IPC: C12Q1/6865 ,  C12Q1/70

Abstract: Nucleic acid oligomeric sequences and in vitro nucleic acid amplification and detection methods for detecting the presence of HAV RNA sequences in samples are disclosed. Kits comprising nucleic acid oligomers for amplifying and detecting HAV nucleic acid sequences are disclosed.

公开(公告)号：US10119163B2

公开(公告)日：2018-11-06

申请号：US15058702

申请日：2016-03-02

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  Norman C. Nelson ,  James D. Carlson ,  Michael M. Becker ,  Lyle J. Arnold, Jr.

IPC: C12Q1/6865 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6853

Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

公开(公告)号：US09469881B2

公开(公告)日：2016-10-18

申请号：US14031353

申请日：2013-09-19

Applicant: Gen-Probe Incorporated

Inventor： James D. Carlson ,  Steven T. Brentano

IPC: C12Q1/68 ,  C12Q1/70

CPC classification number: C12Q1/6865 ,  C12Q1/706 ,  C12Q2600/158 ,  C12Q2600/16 ,  Y02A50/451 ,  Y02A50/54

Abstract: Nucleic acid oligomeric sequences and in vitro nucleic acid amplification and detection methods for detecting the presence of HAV RNA sequences in samples are disclosed. Kits comprising nucleic acid oligomers for amplifying and detecting HAV nucleic acid sequences are disclosed.