公开(公告)号：US09429507B2

公开(公告)日：2016-08-30

申请号：US14390276

申请日：2013-03-13

Applicant: Hitachi High-Technologies Corporation

Inventor： Takuya Matsui ,  Muneo Maeshima

IPC: G01N21/75 ,  G01N15/14 ,  C12M1/34 ,  C12M1/00 ,  B01L3/00 ,  G01N33/483 ,  G01N15/10

CPC classification number: G01N15/1404 ,  B01L3/502753 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2400/0487 ,  B01L2400/086 ,  C12M41/36 ,  C12M47/02 ,  G01N33/4833 ,  G01N2015/1006 ,  G01N2015/1415

Abstract: All of bio-related substances, such as cells or bacteria, are placed at single and independent positions. A flow cell according to the present invention is used for analyzing a bio-related substance and includes a flow passageway and an injection opening and a discharge opening that are connected to the flow passageway. The flow passageway is provided with trapping structural members for trapping the bio-related substance. The trapping structural members include a structure forming a dead water region in which the bio-related substance is trapped.

Abstract translation: 所有生物相关物质，如细胞或细菌，都放置在单独的和独立的位置。 根据本发明的流通池用于分析生物相关物质，并且包括连接到流动通道的流动通道和注射开口和排出口。 流动通道设置有用于捕获生物相关物质的捕获结构构件。 捕获结构构件包括形成生物相关物质被捕获的死水区域的结构。

公开(公告)号：US09023282B2

公开(公告)日：2015-05-05

申请号：US14484384

申请日：2014-09-12

Applicant: Hitachi High-Technologies Corporation

Inventor： Sakuichiro Adachi ,  Muneo Maeshima ,  Isao Yamazaki ,  Tomonori Mimura

IPC: G01N33/00 ,  G01N21/51

CPC classification number: G01N21/51 ,  G01N15/0205 ,  G01N21/253 ,  G01N2015/0065 ,  G01N2035/00396 ,  G01N2035/0453

Abstract: To be adapted to various types of latex reagents for detecting scattered light and thereby measuring agglutination reactions with high sensitivity while sufficiently ensuring integration time. To be adapted to various types of latex particles of different particle sizes, a plurality of light receivers are arranged in a plane perpendicular to the direction of cell movement by rotation of a cell disk. To ensure sufficient integration time, the angle between the optical axis of the irradiation light and each of a plurality of optical axes of scattered light viewed from above the cell is made equal to or less than 17.7° including a mounting error.

Abstract translation: 适用于各种类型的用于检测散射光的乳胶试剂，从而以高灵敏度测量凝集反应，同时充分确保积分时间。 为了适应于各种类型的不同粒度的胶乳颗粒，多个光接收器通过旋转细胞盘而布置在垂直于细胞移动方向的平面中。 为了确保足够的积分时间，使得照射光的光轴与从电池单元的上方观察的散射光的多个光轴中的每一个之间的角度等于或小于包括安装误差的17.7°。

公开(公告)号：US20140377853A1

公开(公告)日：2014-12-25

申请号：US14484384

申请日：2014-09-12

Applicant: Hitachi High-Technologies Corporation

Inventor： Sakuichiro Adachi ,  Muneo Maeshima ,  Isao Yamazaki ,  Tomonori Mimura

IPC: G01N21/51

CPC classification number: G01N21/51 ,  G01N15/0205 ,  G01N21/253 ,  G01N2015/0065 ,  G01N2035/00396 ,  G01N2035/0453

Abstract: To be adapted to various types of latex reagents for detecting scattered light and thereby measuring agglutination reactions with high sensitivity while sufficiently ensuring integration time. To be adapted to various types of latex particles of different particle sizes, a plurality of light receivers are arranged in a plane perpendicular to the direction of cell movement by rotation of a cell disk. To ensure sufficient integration time, the angle between the optical axis of the irradiation light and each of a plurality of optical axes of scattered light viewed from above the cell is made equal to or less than 17.7° including a mounting error.

Abstract translation: 适用于各种类型的用于检测散射光的乳胶试剂，从而以高灵敏度测量凝集反应，同时充分确保积分时间。 为了适应于各种类型的不同粒度的胶乳颗粒，多个光接收器通过旋转细胞盘而布置在垂直于细胞移动方向的平面中。 为了确保足够的积分时间，使得照射光的光轴与从电池单元的上方观察的散射光的多个光轴中的每一个之间的角度等于或小于包括安装误差的17.7°。

公开(公告)号：US20140295430A1

公开(公告)日：2014-10-02

申请号：US14349520

申请日：2012-10-04

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

IPC: C12Q1/68 ,  G01N33/543

CPC classification number: C12Q1/6834 ,  C12Q1/6804 ,  C12Q1/6837 ,  G01N21/6428 ,  G01N21/6452 ,  G01N33/54326 ,  G01N33/54346 ,  G01N33/54393 ,  G01N33/587 ,  G01N2021/6421 ,  C12Q2525/161 ,  C12Q2545/114 ,  C12Q2563/107 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2565/514 ,  C12Q2563/119

Abstract: The method for analyzing biomolecules, includes the steps of: immobilizing biomolecules to be analyzed on surfaces of magnetic microparticles; reacting labeled probe molecules with the biomolecules to be analyzed; collecting and immobilizing the microparticles on a support substrate; and measuring a label on the support substrate. Since single-molecule immobilized magnetic microparticles are used in the present invention, the number of biomolecules can be counted, and since hybridization and an antigen-antibody reaction are performed with the microparticles having biomolecules immobilized thereon dispersed, the reaction can be rapidly performed. Further, the type and the abundance of biomolecules of interest can be determined at a single molecular level, so as to evaluate, in particular, the absolute concentration of biomolecules.

Abstract translation: 分析生物分子的方法包括以下步骤：将待分析的生物分子固定在磁性微粒的表面上; 使标记的探针分子与待分析的生物分子反应; 将微粒收集并固定在支撑基底上; 并测量支撑衬底上的标签。 由于在本发明中使用单分子固定化磁性微粒，因此可以计数生物分子的数量，由于在其上分散固定有生物分子的微粒进行杂交和抗原 - 抗体反应，所以可以快速进行反应。 此外，感兴趣的生物分子的类型和丰度可以在单个分子水平上确定，以便特别地评估生物分子的绝对浓度。