公开(公告)号：US07109322B2

公开(公告)日：2006-09-19

申请号：US10254845

申请日：2002-09-26

Applicant: Metin Colpan ,  Peter Moritz ,  Joachim Schorr

Inventor： Metin Colpan ,  Peter Moritz ,  Joachim Schorr

IPC: C07H21/00 ,  C12N1/00 ,  C12N1/08 ,  C12P19/34

CPC classification number: B01D15/363 ,  B01D15/3804 ,  C12N15/101 ,  C12N15/87 ,  Y10S435/81 ,  Y10S435/82 ,  Y10S436/808 ,  Y10T436/10 ,  G01N30/461

Abstract: A process for the depletion or removal of endotoxins from preparations containing active ingredients designated for therapeutical use which are obtained from natural sources by genetic engineering and/or biotechnology by treatment with chromatographic material wherein said natural source are lysed, the fractions obtained are optionally centrifuged, filtrated or treated with affinity chromatographic methods; said fractions are preincubated with an aqueous salt solution and detergents, treated with anion exchange material and then washed with another salt solution, and the active ingredients are eluted from the anion exchanger, followed by further purification in a per se known manner.

公开(公告)号：US5652141A

公开(公告)日：1997-07-29

申请号：US442100

申请日：1995-05-16

Applicant: Karsten Henco ,  Metin Colpan ,  Petra Feuser

Inventor： Karsten Henco ,  Metin Colpan ,  Petra Feuser

IPC: C12N15/10 ,  C12Q1/68 ,  C12N1/08 ,  C07H1/06

CPC classification number: C12N15/101 ,  C12N15/1006 ,  C12Q1/6806

Abstract: A device and a process for isolating nucleic acids by lysing intact cells and removing nucleic acids emerging from the lysed cells by the following steps:a) the cells are immobilized in a porous matrix, with the size of matrix voids being in the range of the type of cell to be lysed;b) the cells are lysed;c) the nucleic acids are fixated on the matrix surface, and subsequentlyd) are eluted.

Abstract translation: 通过以下步骤裂解完整细胞和从裂解的细胞中除去出现的核酸来分离核酸的装置和方法：a）将细胞固定在多孔基质中，其中基质空隙的大小在 待裂解的细胞类型; b）细胞裂解; c）将核酸固定在基质表面上，随后d）被洗脱。

公开(公告)号：US4699717A

公开(公告)日：1987-10-13

申请号：US830708

申请日：1986-02-14

Applicant: Detlev Riesner ,  Metin Colpan

Inventor： Detlev Riesner ,  Metin Colpan

IPC: B01D15/08 ,  B01J20/28 ,  B01J20/32

CPC classification number: B01D15/08 ,  B01J20/103 ,  B01J20/28004 ,  B01J20/28057 ,  B01J20/28085 ,  B01J20/28095 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3219 ,  B01J20/3257 ,  B01J20/3259 ,  B01J20/3263 ,  B01D15/34 ,  B01D15/362 ,  B01D15/363 ,  B01J2220/54 ,  B01J2220/58

Abstract: A process for the chromatographic separation of nucleic acid using a chromatographic carrier material is described in which the surface of the carrier material is specially modified.

Abstract translation: 描述了使用色谱载体材料色谱分离核酸的方法，其中载体材料的表面被特别改性。

公开(公告)号：US06180778B2

公开(公告)日：2001-01-30

申请号：US08687610

申请日：1996-08-08

Applicant: Helge Bastian ,  Simone Gauch ,  Metin Colpan ,  Petra Feuser

Inventor： Helge Bastian ,  Simone Gauch ,  Metin Colpan ,  Petra Feuser

IPC: C07H2100

CPC classification number: C12N15/1006 ,  C12N15/101 ,  C12Q1/6806

Abstract: The present invention pertains to a process for the chromatographic separation of nucleic acid mixtures into their double-stranded and single-stranded nucleic acid fractions by simultaneously absorbing said nucleic acids as a whole to a mineral support, followed by separation into double-stranded and single-stranded nucleic acids by fractional elution, or by selectively absorbing double-stranded or single-stranded nucleic acid of a liquid sample to a mineral support, as well as solutions and a kit for performing the process according to the invention.

Abstract translation: 本发明涉及通过将所述核酸整体同时吸收到矿物载体上，然后将其分离成双链和单链核酸的单链和单链核酸部分，将核酸混合物色谱分离成双链和单链核酸部分 通过分批洗脱或通过选择性地将液体样品的双链或单链核酸吸收到矿物载体，以及用于执行根据本发明的方法的溶液和试剂盒来代替。

公开(公告)号：US5792651A

公开(公告)日：1998-08-11

申请号：US687530

申请日：1996-10-11

Applicant: Metin Colpan ,  Joachim Schorr

Inventor： Metin Colpan ,  Joachim Schorr

IPC: C12N15/09 ,  A61K48/00 ,  C12N1/21 ,  C12N5/10 ,  C12N15/10 ,  C12N15/87 ,  C12N1/06

CPC classification number: C12N15/87 ,  C12N15/101

Abstract: Use of isopropanol in aqueous solutions for chromatographic isolation of nucleic acids for enhancing the transfection efficiency of the isolated nucleic acids in prokaryotic and eukaryotic cells.

Abstract translation: PCT No.PCT / EP95 / 00390 Sec。 371日期：1996年10月11日 102（e）日期1996年10月11日PCT提交1995年2月3日PCT公布。 WO95 / 21178 PCT出版物 日期1995年8月10日使用用于色谱分离核酸的水溶液中的异丙醇，用于提高分离的核酸在原核和真核细胞中的转染效率。

公开(公告)号：US5747663A

公开(公告)日：1998-05-05

申请号：US687522

申请日：1996-09-30

Applicant: Metin Colpan ,  Peter Moritz ,  Joachim Schorr

Inventor： Metin Colpan ,  Peter Moritz ,  Joachim Schorr

IPC: B01J20/281 ,  A61K31/70 ,  A61K31/7088 ,  A61K48/00 ,  A61P35/00 ,  A61P43/00 ,  B01J20/26 ,  B01J41/04 ,  C07H1/08 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/10 ,  C12N15/87 ,  G01N30/88 ,  C12P19/34 ,  C12N1/06

CPC classification number: B01D15/363 ,  B01D15/3804 ,  C12N15/101 ,  C12N15/87 ,  Y10S435/81 ,  Y10S435/82 ,  Y10S436/808 ,  Y10T436/10

Abstract: A process is disclosed for reducing or removing endotoxins from compositions containing therapeutic active substances extracted from natural sources by genetic engineering and/or biotechnology. For that purpose, the compositions are treated with chromatographic materials. The natural sources are disintegrated, the thus obtained fractions are, if required, centrifuged, filtered or treated using affinity chromatography methods, the fractions are pre-incubated in an aqueous salt solution and detergents, are treated with anion exchange materials, then washed with another salt solution. The active substances are eluted from the anion exchanger then further purified in a manner known per se.

Abstract translation: PCT No.PCT / EP95 / 00391 Sec。 371日期1996年9月30日 102（e）1996年9月30日PCT提交1995年2月3日PCT公布。 WO95 / 21179 PCT公开号 日期1995年8月10日公开了一种通过基因工程和/或生物技术从含有从天然来源提取的治疗活性物质的组合物中还原或去除内毒素的方法。 为此，用色谱材料处理组合物。 将天然来源分解，如果需要，如此获得的级分用亲和层析方法离心，过滤或处理，将级分在盐水溶液和洗涤剂中预温育，用阴离子交换材料处理，然后用另一种 盐溶液。 活性物质从阴离子交换剂洗脱，然后以本身已知的方式进一步纯化。

公开(公告)号：US5057426A

公开(公告)日：1991-10-15

申请号：US123698

申请日：1987-11-23

Applicant: Karsten Henco ,  Arndt Stichel ,  Metin Colpan

Inventor： Karsten Henco ,  Arndt Stichel ,  Metin Colpan

IPC: B01J20/281 ,  A61K38/00 ,  A61K39/395 ,  B01J20/00 ,  C07H1/08 ,  C07H21/00 ,  C07H21/04 ,  C12N15/10 ,  G01N30/60 ,  G01N30/88

CPC classification number: G01N30/6052 ,  C12N15/101 ,  G01N2030/8813 ,  G01N2030/8827 ,  G01N2030/8831 ,  G01N2030/885 ,  G01N30/6065 ,  G01N30/6091

Abstract: A method for the separation of long-chain nucleic acids from other substances in solutions containing nucleic acids and other materials, comprising fixing long-chain nucleic acids in a nucleic acid-containing solution onto a porous matrix, washing the porous matrix to separate the other substances from the long-chain nucleic acids, and removing the fixed long-chain nucleic acids from the porous matrix is disclosed. A device for carrying out the method of the claimed invention is also described.

Abstract translation: 一种用于在含有核酸和其他材料的溶液中从其它物质中分离长链核酸的方法，包括将含核酸溶液中的长链核酸固定在多孔基质上，洗涤多孔基质以分离另一种 公开了来自长链核酸的物质，以及从多孔基质中除去固定的长链核酸。 还描述了用于实现要求保护的发明的方法的装置。

公开(公告)号：US20060194304A1

公开(公告)日：2006-08-31

申请号：US11412130

申请日：2006-04-27

Applicant: Metin Colpan ,  Peter Moritz ,  Joachim Schorr

Inventor： Metin Colpan ,  Peter Moritz ,  Joachim Schorr

IPC: C02F3/34

CPC classification number: B01D15/363 ,  B01D15/3804 ,  C12N15/101 ,  C12N15/87 ,  Y10S435/81 ,  Y10S435/82 ,  Y10S436/808 ,  Y10T436/10 ,  G01N30/461

Abstract: A process for the depletion or removal of endotoxins from preparations containing active ingredients designated for therapeutical use which are obtained from natural sources by genetic engineering and/or biotechnology by treatment with chromatographic material wherein said natural sources are lysed, the fractions obtained are optionally centrifuged, filtrated or treated with affinity chromatographic methods; said fractions are preincubated with an aqueous salt solution and detergents, treated with anion exchange material and then washed with another salt solution, and the active ingredients are eluted from the anion exchanger, followed by further purification in a per se known manner.

公开(公告)号：US07074916B2

公开(公告)日：2006-07-11

申请号：US10340834

申请日：2003-01-13

Applicant: Helge Bastian ,  Simone Gauch ,  Metin Colpan ,  Petra Feuser

Inventor： Helge Bastian ,  Simone Gauch ,  Metin Colpan ,  Petra Feuser

IPC: C07H21/04 ,  C12Q1/68 ,  C12P19/34

CPC classification number: C12N15/1006 ,  C12N15/101 ,  C12Q1/6806

Abstract: The present invention pertains to a process for the chromatographic separation of nucleic acid mixtures into their double-stranded and single-stranded nucleic acid fractions by simultaneously absorbing said nucleic acids as a whole to a mineral support, followed by separation into double-stranded and single-stranded nucleic acids by fractional elution, or by selectively absorbing double-stranded or single-stranded nucleic acid of a liquid sample to a mineral support, as well as solutions and a kit for performing the process according to the invention.

公开(公告)号：US06609618B2

公开(公告)日：2003-08-26

申请号：US09900199

申请日：2001-07-09

Applicant: Metin Colpan

Inventor： Metin Colpan

IPC: B01D2900

CPC classification number: C12N15/101 ,  B01D39/00 ,  C12N15/1017 ,  Y10T436/25375 ,  Y10T436/255

Abstract: Described is a method of isolating cell components, such as nucleic acids, from natural sources by filtering a sample of the digested natural sources such as cells or cell fragments. The method is characterized in that the sample is passed through a filter, the pore size of which decreases in the direction of flow of the sample through the filter.

Abstract translation: 描述了通过过滤消化的天然来源（例如细胞或细胞碎片）的样品从天然来源分离细胞成分（例如核酸）的方法。 该方法的特征在于，样品通过过滤器，其孔径在样品通过过滤器的流动方向上减小。