公开(公告)号：US20090305327A1

公开(公告)日：2009-12-10

申请号：US12224388

申请日：2007-02-15

Applicant: Jochen Franzen ,  Karsten Michelmann ,  Markus Kostrzewa

Inventor： Jochen Franzen ,  Karsten Michelmann ,  Markus Kostrzewa

IPC: C12Q1/02 ,  C12Q1/00

CPC classification number: G01N33/6848 ,  C12Q1/37 ,  C12Q1/56 ,  G01N2333/75 ,  G01N2333/976

Abstract: The invention relates to the determination of the nature and strength of enzymatic activity in blood using mass spectrometric measurement of a profile of the reaction products. The determination of the enzymatic activity can be used for medical diagnostics, for example, and also to check the effectiveness of medication. The invention provides a method whereby adding probe substances usually not present in blood offers standardized substrates for measuring the enzymatic activity. The probe substances may be added to whole blood, plasma, or serum. The mass spectrometric measurement of the reaction products, after their reversible immobilization on actively binding surfaces of solids, for example, can deliver biomarker patterns of the reaction products which may be indicators for metabolic anomalies or diseases, since these are often accompanied by the formation or activation of characteristic enzymes.

Abstract translation: 本发明涉及使用反应产物分布的质谱测量来测定血液中酶活性的性质和强度。 酶活性的测定可以用于医学诊断，例如，以及检查药物的有效性。 本发明提供了通过添加通常不存在于血液中的探针物质来提供用于测量酶活性的标准化底物的方法。 探针物质可以添加到全血，血浆或血清中。 反应产物在其可固定地固定在固体的活性结合表面上之后的质谱测量可以提供反应产物的生物标志物模式，其可以是代谢异常或疾病的指标，因为它们通常伴随着形成或 特征酶的活化。

公开(公告)号：US07615343B2

公开(公告)日：2009-11-10

申请号：US10824656

申请日：2004-04-14

Applicant: Jochen Franzen ,  Hans-Jakob Baum

Inventor： Jochen Franzen ,  Hans-Jakob Baum

IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/00

CPC classification number: C12Q1/6816 ,  G01N27/3278 ,  C12Q2563/143 ,  C12Q2565/607

Abstract: The invention relates to the detection of the binding of analyte molecules, for example biopolymer molecules, to immobilized capture substance molecules. The invention consists in using semiconductor wafers (chips) with electrical circuits in spatial proximity to a surface area coated with capture substance molecules and loading the binding of the analyte molecules to the capture substance molecules with co-bound electrically conductive nanoparticles so that the nanoparticles can act upon the electrical circuits either through changes in the electrical stray capacitance or by generating electric currents, thus making the binding of the analyte molecules electronically detectable.

Abstract translation: 本发明涉及检测分析物分子例如生物聚合物分子与固定的捕获物质分子的结合。 本发明包括使用半导体晶片（芯片）与电路在空间上接近涂覆有捕获物质分子的表面区域，并将分析物分子与共结合导电纳米粒子的结合加载，使得纳米颗粒可以 通过电杂散电容的变化或通过产生电流对电路施加作用，从而使分析物分子的电子可检测结合。

公开(公告)号：US20090039283A1

公开(公告)日：2009-02-12

申请号：US11522112

申请日：2006-09-15

Applicant: Jochen Franzen ,  Karsten Michelmann

Inventor： Jochen Franzen ,  Karsten Michelmann

IPC: H01J27/00

CPC classification number: G01N27/62 ,  H01J49/0463 ,  H01J49/0481 ,  H01J49/145

Abstract: An ion source generates ions from analyte molecules which are desorbed from a sample on the surface of a sample support in a pressure range of approximately 30 to 300 pascal. Reactant ions are generated in a separate ion source and guided by ion guides to the point in front of the sample or to a reaction chamber in which the desorbed molecules are located. The reactant ions ionize the desorbed molecules to form analyte ions. The analyte molecules can be mixed in matrix material or adsorbed on the sample support surface without additives. The desorption can be continuous or pulsed, for example by light from lasers or diodes.

Abstract translation: 离子源从分析物分子产生离子，其在约30至300帕斯卡的压力范围内从样品载体表面上的样品解吸。 反应物离子在单独的离子源中产生并由离子导向器引导到样品前面的点或解吸的分子位于其中的反应室。 反应物离子离子解吸分子形成分析离子。 分析物分子可以在基质材料中混合或吸附在样品支持体表面上而无添加剂。 解吸可以是连续的或脉冲的，例如通过来自激光器或二极管的光。

公开(公告)号：US07465940B2

公开(公告)日：2008-12-16

申请号：US11264657

申请日：2005-11-01

Applicant: Jochen Franzen

Inventor： Jochen Franzen

IPC: H01J49/42 ,  H01J49/00 ,  B01D59/44

CPC classification number: H01J49/165 ,  G01N27/68 ,  H01J49/0404 ,  H01J49/044

Abstract: The invention relates to methods and instruments for ionizing analyte molecules, preferably biomolecules, which are dissolved in liquids or firmly adsorbed on surfaces. Liquids are nebulized at atmospheric pressure by electrospraying. Highly charged microdroplets, which enter the vacuum of the mass spectrometer through the inlet capillary, strike an impact plate when energy is fed in. The repulsive Coulomb force of the charges, the absorption of additional thermal energy and/or the conversion of their kinetic energy into thermal energy cause the microdroplets to burst and evaporate. Analyte molecules which are located in the nebulized liquid or on the impact plate are released in charged form and can be fed to the mass spectrometer for analysis by the extraction and collection effect of an ion funnel operated with RF and DC voltages.

Abstract translation: 本发明涉及用于电离被分析物分子（优选生物分子）的方法和仪器，它们溶于液体或牢固地吸附在表面上。 液体通过电喷雾在大气压下雾化。 通过入口毛细管进入质谱仪的真空的高电荷微滴在进入能量时撞击冲击板。电荷的排斥库仑力，附加热能的吸收和/或其动能转化 进入热能导致微滴爆裂和蒸发。 位于雾化液体或冲击板上的分析物分子以带电的形式释放，并且可以通过用RF和DC电压操作的离子漏斗的提取和收集效应进料到质谱仪进行分析。

公开(公告)号：US20080251712A1

公开(公告)日：2008-10-16

申请号：US12052267

申请日：2008-03-20

Applicant: Ian Sanders ,  Gokhan Baykut ,  Jochen Franzen

Inventor： Ian Sanders ,  Gokhan Baykut ,  Jochen Franzen

IPC: B01D59/44

CPC classification number: H01J49/004 ,  G01N27/622

Abstract: The mobility of mass-selected ions in gases is measured at pressures of a few hectopascal by selecting the ions under investigation in a quadrupole filter according to their mass-to-charge ratio m/z, measuring their mobility in a drift region at a pressure of a few hundred Pascal under the influence of a DC electric field and then filtering the measured ions by means of a quadrupole field in order to eliminate, or detect changes in, the mass-to-charge ratio. Several embodiments for the drift region are disclosed, in which the ions are kept in the axis of the drift region by RF fields. As these drift regions can also be utilized for a collision-induced decomposition of the ions, the device can additionally be used as a so-called triple quadrupole mass spectrometer.

Abstract translation: 质量选择的离子在气体中的迁移率通过在四极滤波器中根据其质荷比m / z选择正在研究的离子来测量几何百帕斯卡的压力，测量它们在压力下的漂移区域中的迁移率 在直流电场的影响下，几百帕斯卡，然后通过四极场过滤测量的离子，以消除或检测质荷比的变化。 公开了漂移区的几个实施例，其中通过RF场将离子保持在漂移区的轴上。 由于这些漂移区域也可以用于离子的碰撞诱导分解，因此该装置可另外用作所谓的三重四极杆质谱仪。

公开(公告)号：US07391021B2

公开(公告)日：2008-06-24

申请号：US11243440

申请日：2005-10-04

Applicant: Carsten Stoermer ,  Andreas Brekenfeld ,  Thomas Wehkamp ,  Jochen Franzen

Inventor： Carsten Stoermer ,  Andreas Brekenfeld ,  Thomas Wehkamp ,  Jochen Franzen

IPC: B01D59/44 ,  H01J49/00

CPC classification number: H01J49/065 ,  H01J49/42

Abstract: The invention relates to RF voltage-operated ion guides based on stacked apertured diaphragms. The invention provides ion guides consisting of diaphragm stacks that permit the ion beam to be shaped in cross-section so that it corresponds to the acceptance profile of the subsequent section of the device, therefore yielding optimal ion transmission. For this purpose, at least some of the diaphragms in the diaphragm stacks do not have circular openings, but instead have openings which shape the cross section of the emerging ion beam in the desired manner. It is possible, for instance, to obtain elliptical beam cross sections, divided beams or beams focused to the shape of a fine thread at the output of the diaphragm stacks.

Abstract translation: 本发明涉及基于堆叠多孔隔膜的射频电压操作离子导向器。 本发明提供了由隔膜叠层组成的离子导向器，其允许离子束的横截面成形，使得它对应于装置的后续部分的接受曲线，因此产生最佳的离子传播。 为此，膜片叠层中的至少一些隔膜不具有圆形开口，而是具有以期望的方式成形出现的离子束横截面的开口。 例如，可以获得椭圆形横截面，在光阑堆叠的输出处聚焦成细螺纹形状的分束或束。

公开(公告)号：US20070084998A1

公开(公告)日：2007-04-19

申请号：US11505237

申请日：2006-08-16

Applicant: Jochen Franzen ,  Carsten Stoermer ,  Bruce Reinhold

Inventor： Jochen Franzen ,  Carsten Stoermer ,  Bruce Reinhold

IPC: H01J49/00

CPC classification number: H01J49/004

Abstract: In a tandem mass spectrometer ions are created only once and stored in an ion reservoir. A particular ion species to be analyzed is then exported from the reservoir through a mass selective ion gate without damaging the other ion species remaining in the reservoir. All subsequent analyses are conducted on these stored ions, without adding further ions so that no changes in the concentrations of the stored ion species occur. The exported ions are fragmented, and a fragment ion mass spectrum is measured in a mass analyzer, preferably in a time-of-flight mass analyzer with orthogonal ion injection. The processes of exporting a selected ion species with subsequent fragmentation and the acquisition of the fragment ion spectrum can be repeated for any number of ion species stored in the reservoir.

Abstract translation: 在串联质谱仪中，离子仅产生一次并存储在离子储存器中。 待分析的特定离子物质然后从储存器通过质量选择性离子门输出，而不损害储存器中剩余的其它离子物质。 对这些储存的离子进行所有后续分析，而不添加其他离子，使得不存在所存储的离子物质的浓度的变化。 输出的离子是碎片，并且在质量分析器中，优选在具有正交离子注入的飞行时质量分析器中测量碎片离子质谱。 可以对储存在储存器中的任何数量的离子物质重复输出选择的离子物质随后的碎裂和片段离子谱的获取的过程。

公开(公告)号：US20060284070A1

公开(公告)日：2006-12-21

申请号：US11440803

申请日：2006-05-25

Applicant: Gokhan Baykut ,  Christian Berg ,  Jochen Franzen

Inventor： Gokhan Baykut ,  Christian Berg ,  Jochen Franzen

IPC: B01D59/44

CPC classification number: H01J49/38

Abstract: The invention relates to methods and devices for regulating the filling level in measuring cells of ion cyclotron resonance mass spectrometers so that it is optimal for mass resolution and mass accuracy. The invention consists in supplying a fraction of the samples to a second reference mass spectrometer operated in parallel, and employing the mass spectra obtained from this reference mass spectrometer to regulate the filling level in the ion cyclotron resonance mass spectrometer.

公开(公告)号：US07030374B2

公开(公告)日：2006-04-18

申请号：US10859924

申请日：2004-06-03

Applicant: Jochen Franzen

Inventor： Jochen Franzen

IPC: H01J39/34

CPC classification number: H01J49/424 ,  H01J49/0054

Abstract: The invention relates to a method and device for the fragmentation of macromolecules, preferably biomolecules, by electron capture in RF quadrupole ion trap mass spectrometers according to Wolfgang Paul. The invention comprises steering a beam of low energy electrons through a magnetic guide field exactly into an ion cloud in the center of the ion trap.

Abstract translation: 本发明涉及根据沃尔夫冈保罗（Wolfgang Paul）的RF四极离子阱质谱仪中的电子俘获来分解大分子，优选生物分子的方法和装置。 本发明包括通过导磁场将低能电子束精确地引导到离子阱中心的离子云中。

公开(公告)号：US20060063145A1

公开(公告)日：2006-03-23

申请号：US11195365

申请日：2005-08-02

Applicant: Detlev Suckau ,  Martin Schurenberg ,  Jochen Franzen

Inventor： Detlev Suckau ,  Martin Schurenberg ,  Jochen Franzen

IPC: C12Q1/00 ,  G06F19/00

CPC classification number: G01N33/483 ,  H01J49/0004 ,  Y10T436/24

Abstract: The invention relates to the determination and visualization of the spatial distribution of tissue states in histologic tissue sections on the basis of mass spectrometric signals acquired so as to be spatially resolved. The invention provides a method which determines the tissue state for the tissue spots as a state characteristic, which is calculated as a mathematical or logical expression from at least two mass signals of this tissue spot, and which indicates the tissue state as a gray-level or false-color image in one or two dimensions.

Abstract translation: 本发明涉及基于获得的空间分辨的质谱信号来确定和可视化组织组织切片中组织状态的空间分布。 本发明提供了一种方法，其将组织斑点的组织状态确定为状态特征，其从该组织斑点的至少两个质量信号计算为数学或逻辑表达，并且将组织状态指示为灰度级 或一维或二维的假彩色图像。