公开(公告)号：US08524057B2

公开(公告)日：2013-09-03

申请号：US13193128

申请日：2011-07-28

Applicant: Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim L. Johnson ,  James M. Bustillo ,  John H. Leamon ,  Jonathan Schultz

Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim L. Johnson ,  James M. Bustillo ,  John H. Leamon ,  Jonathan Schultz

IPC: G01N27/414

CPC classification number: C12Q1/6874 ,  B01L3/502761 ,  B01L2300/046 ,  B01L2300/0663 ,  B01L2300/0851 ,  B01L2400/086 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N27/4148 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2565/629

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

公开(公告)号：US20120027334A1

公开(公告)日：2012-02-02

申请号：US13253476

申请日：2011-10-05

Applicant: Daniel Putt ,  Jonathan Schultz

Inventor： Daniel Putt ,  Jonathan Schultz

IPC: F16C43/04

CPC classification number: F16D1/096 ,  F16C19/38 ,  F16C23/086 ,  F16C35/073 ,  Y10T29/49 ,  Y10T29/49636 ,  Y10T29/49696 ,  Y10T29/49698 ,  Y10T29/49947 ,  Y10T29/53104 ,  Y10T403/5793 ,  Y10T403/7069

Abstract: A mechanism and method for locking a bearing to a shaft includes a split sleeve and a receptive flange adapted to be fixed to the bearing. A positioning flange is coupled to the split sleeve. A screw extends through the positioning flange and threadingly engages the receptive flange. Rotation of the screw in a first direction axially drives the sleeve into engagement with the bearing to collapse the split sleeve into engagement with the shaft.

Abstract translation: 用于将轴承锁定到轴的机构和方法包括分开套筒和适于固定到轴承的容纳凸缘。 定位凸缘与分开的套筒相连。 螺钉延伸穿过定位凸缘并与接收凸缘螺纹接合。 螺杆在第一方向的旋转轴向地驱动套筒与轴承接合，以使分开的套筒与轴接合。

公开(公告)号：US20080235933A1

公开(公告)日：2008-10-02

申请号：US12052142

申请日：2008-03-20

Applicant: Daniel Putt ,  Jonathan Schultz

Inventor： Daniel Putt ,  Jonathan Schultz

IPC: B23P19/02

CPC classification number: F16D1/096 ,  F16C19/38 ,  F16C23/086 ,  F16C35/073 ,  Y10T29/49 ,  Y10T29/49636 ,  Y10T29/49696 ,  Y10T29/49698 ,  Y10T29/49947 ,  Y10T29/53104 ,  Y10T403/5793 ,  Y10T403/7069

Abstract: A mechanism and method for locking a bearing to a shaft includes a split sleeve and a receptive flange adapted to be fixed to the bearing. A positioning flange is coupled to the split sleeve. A screw extends through the positioning flange and threadingly engages the receptive flange. Rotation of the screw in a first direction axially drives the sleeve into engagement with the bearing to collapse the split sleeve into engagement with the shaft.

Abstract translation: 用于将轴承锁定到轴的机构和方法包括分开套筒和适于固定到轴承的容纳凸缘。 定位凸缘与分开的套筒相连。 螺钉延伸穿过定位凸缘并与接收凸缘螺纹接合。 螺杆在第一方向的旋转轴向地驱动套筒与轴承接合，以使分开的套筒与轴接合。

公开(公告)号：US12256793B2

公开(公告)日：2025-03-25

申请号：US17477264

申请日：2021-09-16

Applicant: Robert Schultz ,  Jonathan Schultz

Inventor： Robert Schultz ,  Jonathan Schultz

IPC: A41D13/12

Abstract: An adjustable hospital gown is designed to cover the bodies of users of varying shape and size. The gown has a torso-covering garment, a pair of backflaps, and an adjustable fastener. The torso-covering garment is used to cover the front side of the user's body. The pair of backflaps are connected along backflap seams that are positioned on opposite sides of the torso-covering garment. As a result, the pair of backflaps can be transitioned between an opened and a closed configuration. The adjustable fastener is connected in between one of the backflaps and the torso-covering garment such that the backflap can be repositioned to cinch the gown around the body of the user. By connecting the adjustable fastener to various anchor points, the size of the gown can be modified to accommodate user's of varying shape.

公开(公告)号：US10146906B2

公开(公告)日：2018-12-04

申请号：US13339846

申请日：2011-12-29

Applicant: Todd Rearick ,  Jonathan Schultz

Inventor： Todd Rearick ,  Jonathan Schultz

IPC: G06F19/24 ,  C12Q1/6869

Abstract: Mathematical models for the analysis of signal data generated by sequencing of a polynucleotide strand using a pH-based method of detecting nucleotide incorporation(s). In an embodiment, the measured output signal from the reaction confinement region of a reactor array is mathematically modeled. The output signal may be modeled as a linear combination of one or more signal components, including a background signal component. This model is solved to determine the nucleotide incorporation signal. In another embodiment, the incorporation signal from the reaction confinement region of a reactor array is mathematically modeled.

公开(公告)号：US09428807B2

公开(公告)日：2016-08-30

申请号：US13440849

申请日：2012-04-05

Applicant: Earl Hubbell ,  Jonathan Schultz

Inventor： Earl Hubbell ,  Jonathan Schultz

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/6874 ,  C12Q2533/101 ,  C12Q2565/301 ,  C12Q2565/607

Abstract: A method for nucleic acid sequencing includes disposing template polynucleotide strands in defined spaces on a sensor array, at least some of the template polynucleotide strands having a sequencing primer and a polymerase operably bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed according to a predetermined ordering; and determining, for each of the series of flows of nucleotide species, how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands, wherein the predetermined ordering (a) is not a series of consecutive repetitions of a 4-flow permutation of four different nucleotide species, (b) is not specifically tailored to a particular combination of a particular template polynucleotide strand to be sequenced and a particular sequencing primer to be used, and (c) comprises a phase-protecting flow ordering.

Abstract translation: 用于核酸测序的方法包括在传感器阵列上的限定空间中设置模板多核苷酸链，至少一些模板多核苷酸链具有测序引物和与之可操作地结合的聚合酶; 将模板多核苷酸链暴露于根据预定排序流动的一系列核苷酸类型的流; 并且对于核苷酸种类的每一系列流程，确定针对该特定流出现多少个核苷酸掺入以确定对应于模板多核苷酸链的预测的核苷酸序列，其中预定顺序（a）不是一系列连续的 重复四种不同核苷酸物种的4流置换，（b）不是特定的针对待测序的特定模板多核苷酸链和特定的待测序列的特定组合而定制的，（c） 保护流量顺序。

公开(公告)号：US09017993B2

公开(公告)日：2015-04-28

申请号：US13442547

申请日：2012-04-09

Applicant: Jonathan Schultz ,  John Nobile ,  Brian Reed ,  Prasanna Thwar ,  Todd Roswech

Inventor： Jonathan Schultz ,  John Nobile ,  Brian Reed ,  Prasanna Thwar ,  Todd Roswech

IPC: C12M1/00 ,  C12M3/00 ,  C12M1/34 ,  C12M1/36 ,  C12M1/38 ,  B01F3/08 ,  B01F5/04 ,  B01F13/00 ,  B04B5/04 ,  B04B7/02 ,  B01L3/00

CPC classification number: B01F5/0602 ,  B01F3/0807 ,  B01F5/0485 ,  B01F13/0059 ,  B01F2215/0037 ,  B01L3/502784 ,  B04B5/0414 ,  B04B7/02

Abstract: An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.

Abstract translation: 提供了一种自动化的在线模板珠粒制备系统，包括膜基乳液生成子系统，乳液PCR（ePCR）热循环板和子系统，以及连续离心乳液破乳和模板珠收集子系统。 乳液生成子系统在制备反相乳液时提供均匀性，并可用于快速且可再现地产生大体积或小体积的反相乳液。 提供一种乳液生成装置，其能够以自动方式向逆循环子系统提供连续的反相乳液流。 ePCR子系统可以连续热循环通过的反相乳液，并包括静态温度区和可消耗的热循环板。 连续离心子系统可以连续地破坏热循环的反相乳液并收集形成在反相乳液的微量微反应器液滴中的模板珠粒。

公开(公告)号：US08470164B2

公开(公告)日：2013-06-25

申请号：US13001182

申请日：2009-06-25

Applicant: Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo ,  John Leamon ,  Jonathan Schultz

Inventor： Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo ,  John Leamon ,  Jonathan Schultz

IPC: G01N27/414 ,  C12Q1/68

CPC classification number: C12Q1/6874 ,  B01L3/502761 ,  B01L2300/046 ,  B01L2300/0663 ,  B01L2300/0851 ,  B01L2400/086 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N27/4148 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2565/629

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

公开(公告)号：US20120258516A1

公开(公告)日：2012-10-11

申请号：US13442547

申请日：2012-04-09

Applicant: Jonathan Schultz ,  John Nobile ,  Brian Reed ,  Prasanna Thwar ,  Todd Roswech

Inventor： Jonathan Schultz ,  John Nobile ,  Brian Reed ,  Prasanna Thwar ,  Todd Roswech

IPC: B01F5/06 ,  C12N9/12

CPC classification number: B01F5/0602 ,  B01F3/0807 ,  B01F5/0485 ,  B01F13/0059 ,  B01F2215/0037 ,  B01L3/502784 ,  B04B5/0414 ,  B04B7/02

Abstract: An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.

Abstract translation: 提供了一种自动化的在线模板珠粒制备系统，包括膜基乳液生成子系统，乳液PCR（ePCR）热循环板和子系统，以及连续离心乳液破乳和模板珠收集子系统。 乳液生成子系统在制备反相乳液时提供均匀性，并可用于快速且可再现地产生大体积或小体积的反相乳液。 提供一种乳液生成装置，其能够以自动方式向逆循环子系统提供连续的反相乳液流。 ePCR子系统可以连续热循环通过的反相乳液，并包括静态温度区和可消耗的热循环板。 连续离心子系统可以连续地破坏热循环的反相乳液并收集形成在反相乳液的微量微反应器液滴中的模板珠粒。

公开(公告)号：US20110251078A1

公开(公告)日：2011-10-13

申请号：US13044330

申请日：2011-03-09

Applicant: Jonathan M. Rothberg ,  John H. Leamon ,  John F. Davidson ,  Antoine M. van Oijen ,  Wolfgang Hinz ,  Melville Davey ,  Bradley Hann ,  Jonathan Schultz

Inventor： Jonathan M. Rothberg ,  John H. Leamon ,  John F. Davidson ,  Antoine M. van Oijen ,  Wolfgang Hinz ,  Melville Davey ,  Bradley Hann ,  Jonathan Schultz

IPC: C40B30/00

CPC classification number: C12Q1/6874 ,  C12Q2523/303 ,  C12Q2565/131 ,  C12Q2525/204 ,  C12Q2521/101 ,  C12Q2535/122

Abstract: The invention provides apparatuses and methods of use thereof for sequencing nucleic acids subjected to a force, and thus considered under tension. The methods may employ but are not dependent upon incorporation of extrinsically detectably labeled nucleotides.