MODELS FOR ANALYZING DATA FROM SEQUENCING-BY-SYNTHESIS OPERATIONS
    5.
    发明申请
    MODELS FOR ANALYZING DATA FROM SEQUENCING-BY-SYNTHESIS OPERATIONS 审中-公开
    用于分析合成数据的模型

    公开(公告)号:US20120172241A1

    公开(公告)日:2012-07-05

    申请号:US13339846

    申请日:2011-12-29

    Abstract: Mathematical models for the analysis of signal data generated by sequencing of a polynucleotide strand using a pH-based method of detecting nucleotide incorporation(s). In an embodiment, the measured output signal from the reaction confinement region of a reactor array is mathematically modeled. The output signal may be modeled as a linear combination of one or more signal components, including a background signal component. This model is solved to determine the nucleotide incorporation signal. In another embodiment, the incorporation signal from the reaction confinement region of a reactor array is mathematically modeled.

    Abstract translation: 用于通过使用基于pH的检测核苷酸掺入的方法测序多核苷酸链产生的信号数据分析的数学模型。 在一个实施例中,来自反应堆阵列的反应限制区域的测量的输出信号在数学上被建模。 输出信号可以被建模为包括背景信号分量的一个或多个信号分量的线性组合。 该模型被解决以确定核苷酸掺入信号。 在另一个实施方案中,来自反应器阵列的反应限制区域的掺入信号在数学上被建模。

    METHODS AND APPARATUS FOR MEASURING ANALYTES USING LARGE SCALE FET ARRAYS
    10.
    发明申请
    METHODS AND APPARATUS FOR MEASURING ANALYTES USING LARGE SCALE FET ARRAYS 有权
    使用大规模FET阵列测量分析仪的方法和装置

    公开(公告)号:US20120247977A1

    公开(公告)日:2012-10-04

    申请号:US13193128

    申请日:2011-07-28

    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

    Abstract translation: 与用于分析物测量的非常大规模的FET阵列相关的方法和装置。 可以使用基于提高测量灵敏度和精度的改进的FET像素和阵列设计的传统CMOS处理技术来制造ChemFET(例如,ISFET)阵列,并且同时促进显着小的像素尺寸和致密阵列。 改进的阵列控制技术提供了从大型和密集阵列的快速数据采集。 可以使用这样的阵列来检测各种化学和/或生物过程中各种分析物类型的存在和/或浓度变化。 在一个示例中,chemFET阵列基于监测无机焦磷酸盐(PPi),氢离子和核苷酸三磷酸盐的浓度变化来促进DNA测序技术。

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