公开(公告)号：US09453264B2

公开(公告)日：2016-09-27

申请号：US14736922

申请日：2015-06-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason Yingjie Liu

IPC: C12Q1/68 ,  C12Q1/70

CPC classification number: C12Q1/701 ,  C12Q1/6816 ,  C12Q1/682 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2527/125 ,  C12Q2531/113 ,  C12Q2561/113

Abstract: Disclosed herein are methods, compositions and kits for the quantification of a nucleic acid target present on a solid support. This entails quantitative real-time polymerase chain reaction wherein minor groove binders are excluded.

公开(公告)号：US10968476B2

公开(公告)日：2021-04-06

申请号：US16403012

申请日：2019-05-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason Yingjie Liu

IPC: C12P19/34 ,  C12Q1/6851 ,  C12Q1/6806 ,  C12Q1/686

Abstract: A workflow for direct qPCR quantification of unprocessed forensic casework samples is disclosed herein. 13pg of DNA has been detected by direct amplification from a paper substrate. Direct qPCR quantification of unprocessed forensic casework samples and direct STR amplification of unprocessed forensic casework samples collected on the same PE-swab will greatly increase forensic laboratory's efficiency and capability.

公开(公告)号：US09932624B2

公开(公告)日：2018-04-03

申请号：US14612183

申请日：2015-02-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason Yingjie Liu

IPC: C12P19/34 ,  C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6806 ,  C12N15/1003

Abstract: A method for selectively recovering nucleic acid from a sperm cell in a sample containing cells of at least a sperm cell and an epithelial cell, and a cell suspension medium comprising extracellular impurities, is provided. The method entails introducing a sample into a vessel, sequestering the cells from the remaining sample components, washing the cells with a washing solution either before or after sequestration, removing the impurities-containing cell suspension medium from the vessel while retaining the cells; lysing selectively cells of the first cell type; and isolating the nucleic acid from the lysed cells. Methods for recovering nucleic acid from the second cell type are also provided.

公开(公告)号：US09804066B2

公开(公告)日：2017-10-31

申请号：US14739899

申请日：2015-06-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael Harrold ,  Lori Hennessy ,  Jason Yingjie Liu ,  Chang Zhong

IPC: C12Q1/68 ,  C12M1/00 ,  C12M1/34 ,  G01N1/30 ,  G06T7/00 ,  A61B5/15 ,  A61B5/151 ,  A61B5/00 ,  A61B5/1172 ,  A61B5/145 ,  G06K9/00 ,  A61B10/00 ,  A61B10/02 ,  A61B5/157

CPC classification number: G01N1/30 ,  A61B5/0077 ,  A61B5/1172 ,  A61B5/1411 ,  A61B5/14514 ,  A61B5/150022 ,  A61B5/150083 ,  A61B5/150091 ,  A61B5/150412 ,  A61B5/150984 ,  A61B5/15105 ,  A61B5/15134 ,  A61B5/157 ,  A61B10/0064 ,  A61B10/0233 ,  G06K9/00013 ,  G06K9/00543 ,  G06K2009/00946 ,  G06T7/0012

Abstract: Systems, methods, and kits are disclosed for collection, labeling and analyzing biological samples containing nucleic acid in conjunction with collecting at least one ridge and valley signature of an individual. Such devices and methods are used in forensic, human identification, access control and screening technologies to rapidly process an individual's identity or determine the identity of an individual.

公开(公告)号：US09719130B2

公开(公告)日：2017-08-01

申请号：US14380543

申请日：2013-02-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason Yingjie Liu

IPC: C12Q1/68 ,  G01N1/30 ,  A61B10/00 ,  A61B10/02

CPC classification number: C12Q1/6806 ,  A61B10/0045 ,  A61B10/02 ,  G01N1/30

Abstract: Devices, methods, and kits are disclosed for collection, labeling and analysis of samples containing a substance of interest. Such devices and methods are used in forensic, human identification, access and importation control, and other investigative technologies to collect even limited amounts of a substance of interest that may be present on a substrate and to facilitate analysis to identify the substance of interest.

公开(公告)号：US09708644B2

公开(公告)日：2017-07-18

申请号：US13774903

申请日：2013-02-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason Yingjie Liu ,  Michael Harrold ,  Chang Zhong ,  Chandani Patel

IPC: C12M1/00 ,  C12Q1/68 ,  C12P19/34 ,  C07H21/04 ,  G01N31/22 ,  G06K9/00 ,  G03B29/00 ,  G01N1/30 ,  A61B10/00 ,  A61B10/02

CPC classification number: C12Q1/6806 ,  A61B10/0045 ,  A61B10/02 ,  G01N1/30

Abstract: Systems, methods, and kits are disclosed for collection, labeling and analyzing biological samples containing nucleic acid in conjunction with collecting at least one ridge and valley signature of an individual. Such devices and methods are used in forensic, human identification, access control and screening technologies to rapidly process an individual's identity or determine the identity of an individual.

公开(公告)号：US09212388B1

公开(公告)日：2015-12-15

申请号：US14318925

申请日：2014-06-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason Yingjie Liu

IPC: C12Q1/68

CPC classification number: C12Q1/701 ,  C12Q1/6816 ,  C12Q1/682 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2527/125 ,  C12Q2531/113 ,  C12Q2561/113

Abstract: Disclosed herein are methods, compositions and kits for the quantification of a nucleic acid target present on a solid support. This entails quantitative real-time polymerase chain reaction wherein minor groove binders are excluded.

Abstract translation: 本文公开了用于定量存在于固体支持物上的核酸靶的方法，组合物和试剂盒。 这需要定量实时聚合酶链反应，其中排除小沟结合剂。