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    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME
    12.
    发明申请
    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-公开

    公开(公告)号：US20200299655A1

    公开(公告)日：2020-09-24

    申请号：US16894469

    申请日：2020-06-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter VANDER HORN ,  Daniel MAZUR ,  Theo NIKIFOROV ,  Mindy LANDES ,  Eileen TOZER

    IPC: C12N9/12 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

    METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION
    13.
    发明申请
    METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20190284597A1

    公开(公告)日：2019-09-19

    申请号：US16308803

    申请日：2017-06-09

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Hua YU ,  Theo NIKIFOROV ,  Abraham ROSENBAUM

    IPC: C12P19/34 ,  C12Q1/6853

    Abstract: In some embodiments, the disclosure relates generally to methods, as well as related compositions and kits for recombinase-mediated nucleic acid amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using at least one blocked primer that contains a 5′ domain, at least one nucleotide that is cleavable by an RNase H enzyme, a 3′ domain, wherein the primer is not extendable by a polymerase, and wherein the 3′ domain has a length of 7-100 nucleotides, for example 10-30 nucleotides. These methods and the use of a blocked primer reduce or eliminate non-specific amplification products, such as primer dimers, which are generated in RPA reactions.

    THIOLATED NUCLEOTIDE ANALOGUES FOR NUCLEIC ACID SYNTHESIS
    16.
    发明申请
    THIOLATED NUCLEOTIDE ANALOGUES FOR NUCLEIC ACID SYNTHESIS 审中-公开
    用于核酸合成的硫酸核苷酸类似物

    公开(公告)号：US20160130644A1

    公开(公告)日：2016-05-12

    申请号：US14937211

    申请日：2015-11-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Steven MENCHEN ,  Theo NIKIFOROV

    IPC: C12Q1/68 ,  G01N27/414

    CPC classification number: C12Q1/6869 ,  G01N27/4145 ,  C12Q2525/125 ,  C12Q2535/113 ,  C12Q2565/301 ,  C12Q2565/607

    Abstract: The present disclosure provide systems, compositions, methods, reagents, kits and products for extending a nucleic acid that includes incorporating a nucleotide residue at a terminus of a nucleic acid using a polymerase enzyme and at least one nucleotide, wherein the at least one nucleotide includes a thiophosphate moiety, and wherein the at least one nucleotide is resistant to hydrolysis by phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of a phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of at least on chelation moiety that is configured to bind an orthophosphate moiety.

    Abstract translation: 本公开提供用于扩展核酸的系统，组合物，方法，试剂，试剂盒和产品，其包括使用聚合酶和至少一个核苷酸在核酸的末端掺入核苷酸残基，其中所述至少一个核苷酸包括 硫代磷酸酯部分，并且其中所述至少一个核苷酸耐磷酸酶水解。 在一些实施方案中，核苷酸掺入可以在磷酸酶的存在下进行。 在一些实施方案中，核苷酸掺入可以在配置成结合正磷酸盐部分的至少一个螯合部分的存在下进行。

    METHODS AND APPARATUS FOR SINGLE MOLECULE SEQUENCING USING ENERGY TRANSFER DETECTION
    17.
    发明申请
    METHODS AND APPARATUS FOR SINGLE MOLECULE SEQUENCING USING ENERGY TRANSFER DETECTION 有权
    使用能量转移检测的单分子序列的方法和装置

    公开(公告)号：US20150118679A1

    公开(公告)日：2015-04-30

    申请号：US14584829

    申请日：2014-12-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Joseph BEECHEM ,  Theo NIKIFOROV ,  Vi-En CHOONG ,  Xinzhan PENG ,  Guobin LUO ,  Cheng-Yao CHEN ,  Michael PREVITE

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  C07H19/20 ,  C12N9/1241 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6818 ,  C12Y207/07 ,  C12Y207/07007 ,  G01N21/6428 ,  G01N33/582 ,  G01N2021/6432

    Abstract: Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.

    Abstract translation: 本文提供了用于核苷酸掺入反应的系统和方法。 该系统包含具有改变的核苷酸掺入动力学并且与能量转移供体部分连接的聚合酶，以及与至少一个能量转移受体部分连接的核苷酸分子。 当核苷酸结合和/或核苷酸掺入期间聚合酶和核苷酸彼此接近时，供体和受体部分进行能量转移。 当供体和受体部分进行能量转移时，它们产生可与核苷酸结合或掺入相关联的能量转移信号。 检测所产生的信号的时间序列或信号的变化可用于确定掺入的核苷酸的顺序，因此可用于推导靶分子的序列。

    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME
    18.
    发明公开
    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-公开

    公开(公告)号：US20240067941A1

    公开(公告)日：2024-02-29

    申请号：US18486337

    申请日：2023-10-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter VANDER HORN ,  Theo NIKIFOROV ,  Daniel MAZUR ,  Eileen TOZER ,  Mindy LANDES

    IPC: C12N9/12 ,  C12Q1/686 ,  C12Q1/6869

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

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