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11.发明授权Recombinant cell clones having increased stability and methods of making and using the same 有权具有增加的稳定性的重组细胞克隆及其制备和使用方法公开(公告)号：US08329465B2
公开(公告)日：2012-12-11
申请号：US12986111
申请日：2011-01-06
申请人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
发明人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
CPC分类号： C12N5/0075 , C12N5/0043 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/50 , C12N2500/76 , C12N2531/00
摘要： Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
摘要翻译： 公开了稳定的重组细胞克隆，其在无血清和无蛋白质的培养基中稳定至少40代，通过在无血清和无蛋白质培养条件下倍增稳定细胞克隆获得的生物质，以及制备重组蛋白质的方法 通过生物量。 此外，本发明涉及一种回收稳定的重组细胞克隆的方法。
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公开(公告)号：US20080064105A1
公开(公告)日：2008-03-13
申请号：US11980833
申请日：2007-10-30
CPC分类号： C12N5/0043 , C12N5/0682 , C12N2500/46 , C12N2500/74 , C12N2500/76 , C12N2500/92
摘要： The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.
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公开(公告)号：US20080009040A1
公开(公告)日：2008-01-10
申请号：US11858844
申请日：2007-09-20
IPC分类号： C12P21/00
CPC分类号： C12N5/0043 , C12N5/0682 , C12N2500/46 , C12N2500/74 , C12N2500/76 , C12N2500/92
摘要： The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.
摘要翻译： 本发明涉及含有多胺和植物和/或酵母衍生的水解产物的无动物蛋白的细胞培养基。 本发明还涉及无动物蛋白质的培养方法，其中细胞可培养，繁殖和传代，而不在培养基中添加补充动物蛋白。 这些方法可用于培养细胞，例如重组细胞或感染病毒的细胞，以及通过细胞培养方法生产生物产物。
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公开(公告)号：US20060094104A1
公开(公告)日：2006-05-04
申请号：US10976399
申请日：2004-10-29
CPC分类号： C12N5/0043 , C12N5/0682 , C12N2500/46 , C12N2500/74 , C12N2500/76 , C12N2500/92
摘要： The present invention relates to animal protein-free cell culture media comprising polyamines and a plant and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.
摘要翻译： 本发明涉及包含多胺和植物和/或酵母衍生的水解产物的无动物蛋白的细胞培养基。 本发明还涉及无动物蛋白质的培养方法，其中细胞可培养，繁殖和传代，而不在培养基中添加补充动物蛋白。 这些方法可用于培养细胞，例如重组细胞或感染病毒的细胞，以及通过细胞培养方法生产生物产物。
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公开(公告)号：US06855535B2
公开(公告)日：2005-02-15
申请号：US10006882
申请日：2001-12-10
申请人： Heidi Meyer , Manfred Reiter , Wolfgang Mundt , Noel Barrett , Friedrich Dorner
发明人： Heidi Meyer , Manfred Reiter , Wolfgang Mundt , Noel Barrett , Friedrich Dorner
CPC分类号： C12N7/00 , A61K39/12 , A61K39/29 , A61K2039/5252 , C12N2770/32434 , C12N2770/32451 , Y02A50/464
摘要： The present invention provides methods of large scale production of Hepatitis A Virus (HAV) on VERO cells bound to microcarrier. The invention also provides for methods of isolation of HAV from the cell culture supernatant of HAV infected VERO cells.
摘要翻译： 本发明提供了在与微载体结合的VERO细胞上大规模生产甲型肝炎病毒（HAV）的方法。 本发明还提供从HAV感染的VERO细胞的细胞培养物上清液中分离HAV的方法。
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公开(公告)号：US5880265A
公开(公告)日：1999-03-09
申请号：US898129
申请日：1997-07-22
申请人： Bernhard Fischer , Artur Mitterer , Friedrich Dorner , Hans-Peter Schwarz , Peter Turecek , Johann Eibl , Falko-Guenter Falkner , Uwe Schlokat , Wolfgang Mundt , Manfred Reiter , Renate Den-Bouwmeester
发明人： Bernhard Fischer , Artur Mitterer , Friedrich Dorner , Hans-Peter Schwarz , Peter Turecek , Johann Eibl , Falko-Guenter Falkner , Uwe Schlokat , Wolfgang Mundt , Manfred Reiter , Renate Den-Bouwmeester
IPC分类号： C12N15/09 , A61K35/14 , A61K38/00 , A61K38/43 , A61P7/04 , C07K1/18 , C07K1/22 , C07K14/47 , C07K14/755 , C12P21/00
CPC分类号： C07K14/755 , A61K38/00
摘要： The invention relates to a method for isolation of highly pure von Willebrand Factor in which recombinant von Willebrand Factor (rvWF) is chromatographically purified by anion exchange chromatography on an anion exchanger of the quaternary amino type in a buffer solution comprising buffer substances and optionally salt.The buffer solutions are preferably free of stabilizers, amino acids and other additives. According to this method, highly pure recombinant vWF can be obtained, which is free from blood plasma proteins, especially free from Factor VIII, and is physiologically active.Further, the invention relates to a pharmaceutical preparation that contains rvWF, which is comprised of multimers with a high structural integrity.
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17.发明授权公开(公告)号：US08080414B2
公开(公告)日：2011-12-20
申请号：US12488465
申请日：2009-06-19
申请人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
发明人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
CPC分类号： C12N5/0075 , C12N5/0043 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/50 , C12N2500/76 , C12N2531/00
摘要： Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
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公开(公告)号：US20110287482A1
公开(公告)日：2011-11-24
申请号：US13198476
申请日：2011-08-04
CPC分类号： C12P21/00 , C07K14/755 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0682 , C12N2500/05 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/60 , C12N2500/76 , C12N2500/92
摘要： A medium is described for the protein-free and serum-free cultivation of cells, especially mammalian cells, whereby the medium contains a proportion of soy hydrolysate.
摘要翻译： 描述了用于无蛋白质和无血清培养细胞，特别是哺乳动物细胞的培养基，由此培养基含有一定比例的大豆水解产物。
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公开(公告)号：US08021881B2
公开(公告)日：2011-09-20
申请号：US11841915
申请日：2007-08-20
IPC分类号： C12N5/07
CPC分类号： C12P21/00 , C07K14/755 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0682 , C12N2500/05 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/60 , C12N2500/76 , C12N2500/92
摘要： A medium is described for the protein-free and serum-free cultivation of cells, especially mammalian cells, whereby the medium contains a proportion of soy hydrolysate.
摘要翻译： 描述了用于无蛋白质和无血清培养细胞，特别是哺乳动物细胞的培养基，由此培养基含有一定比例的大豆水解产物。
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公开(公告)号：US20110081722A1
公开(公告)日：2011-04-07
申请号：US12781661
申请日：2010-05-17
IPC分类号： C12N5/071
CPC分类号： C12N5/0043 , C12N5/0682 , C12N2500/46 , C12N2500/74 , C12N2500/76 , C12N2500/92
摘要： The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.
摘要翻译： 本发明涉及含有多胺和植物和/或酵母衍生的水解产物的无动物蛋白的细胞培养基。 本发明还涉及无动物蛋白质的培养方法，其中细胞可培养，繁殖和传代，而不在培养基中添加补充动物蛋白。 这些方法可用于培养细胞，例如重组细胞或感染病毒的细胞，以及通过细胞培养方法生产生物产物。
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