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36 条记录 (耗时 0.032 秒)

    Methods for assembly of high fidelity synthetic polynucleotides
    11.
    发明申请
    Methods for assembly of high fidelity synthetic polynucleotides 审中-公开
    装配高保真合成多核苷酸的方法

    公开(公告)号：US20060194214A1

    公开(公告)日：2006-08-31

    申请号：US11068321

    申请日：2005-02-28

    申请人： George Church ,  Joseph Jacobson ,  Brian Baynes

    发明人： George Church ,  Joseph Jacobson ,  Brian Baynes

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/6813 ,  C12Q2523/305 ,  C12Q2525/131 ,  C12Q2525/155

    摘要： Disclosed are methods of manufacturing synthetic DNAs, that is, DNAs made at least in significant part by chemical synthesis of polynucleotide polymers. Also provided are methods for assembling plural DNAs in the same pool by multiplexed assembly of synthetic oligonucleotides. In exemplary embodiments, the methods involve pre-amplification of one or more oligonucleotides using “universal” primers, reduction of the error rate in oligonucleotide and/or polynucleotide products, and sequence optimization and oligonucleotides design.

    摘要翻译： 公开了制造合成DNA的方法，即通过化学合成多核苷酸聚合物至少部分制备的DNA。 还提供了通过合成寡核苷酸的多重组装在同一池中组装多个DNA的方法。 在示例性实施方案中，所述方法包括使用“通用”引物预扩增一个或多个寡核苷酸，降低寡核苷酸和/或多核苷酸产物中的错误率，以及序列优化和寡核苷酸设计。

    Modular molecular clasps and uses thereof
    14.
    发明申请
    Modular molecular clasps and uses thereof 审中-公开
    模块化分子钩扣及其用途

    公开(公告)号：US20070276129A1

    公开(公告)日：2007-11-29

    申请号：US11505630

    申请日：2006-08-17

    申请人： Carlo Rizzuto ,  Noubar Afeyan ,  Frank Lee ,  George Church ,  Ruchira Gupta ,  John Schwartz ,  Bin Zhang ,  Alexey Lugovskoy

    发明人： Carlo Rizzuto ,  Noubar Afeyan ,  Frank Lee ,  George Church ,  Ruchira Gupta ,  John Schwartz ,  Bin Zhang ,  Alexey Lugovskoy

    IPC分类号： C07K16/46

    CPC分类号： C12N15/62 ,  A61K38/00 ,  C07K19/00 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/30 ,  C07K2319/60 ,  C07K2319/74 ,  G01N33/542

    摘要： The present invention provides Modular Molecular Clasps and methods of using these Modular Molecular Clasps in a wide range of applications in the health care industry, e.g., in therapy, in clinical diagnostics, in in vivo imaging or in drug discovery. The Modular Molecular Clasps of the present invention also have industrial and environmental applications, e.g., in environmental diagnostics, industrial diagnostics, food safety, toxicology, catalysis of reactions or high-throughput screening; as well as applications in the agricultural industry and in basic research. Methods of designing the Modular Molecular Clasps, as well as arrays and biosensors comprising these Modular Molecular Clasps are also provided.

    摘要翻译： 本发明提供了在医疗保健行业的广泛应用中使用这些模块化分子克隆的模块化分子克隆和方法，例如在治疗，临床诊断，体内成像或药物发现中。 本发明的模块化分子克隆还具有工业和环境应用，例如在环境诊断，工业诊断，食品安全，毒理学，反应催化或高通量筛选中。 以及农业行业的应用和基础研究。 还提供了设计模块化分子克隆的方法，以及包含这些模块分子克隆的阵列和生物传感器。

    IN VIVO ALTERATION OF CELLULAR DNA
    15.
    发明申请
    IN VIVO ALTERATION OF CELLULAR DNA 审中-公开
    细胞DNA的体内变异

    公开(公告)号：US20070243616A1

    公开(公告)日：2007-10-18

    申请号：US11749489

    申请日：2007-05-16

    申请人： George Church ,  Nikos Reppas

    发明人： George Church ,  Nikos Reppas

    IPC分类号： C12N15/87 ,  C12N15/00

    CPC分类号： C12N15/90 ,  A01K2217/05 ,  A01K2217/075 ,  C12N15/902

    摘要： The present invention relates to methods, cells and compositions for performing in vivo recombination without the need for clonal selection. These methods, cells and compositions are useful for the automation of recombination. The present invention provides methods for performing sequential steps in genome engineering, as well as for achieving parallel and multiplex genome engineering.

    摘要翻译： 本发明涉及用于进行体内重组而不需要克隆选择的方法，细胞和组合物。 这些方法，细胞和组合物可用于重组的自动化。 本发明提供了用于在基因组工程中执行顺序步骤以及实现并行和多重基因组工程的方法。

    Labeling compositions and methods of use for deterrent trackability
    16.
    发明申请
    Labeling compositions and methods of use for deterrent trackability 审中-公开
    用于威慑可追踪性的标记组合物和使用方法

    公开(公告)号：US20070172429A1

    公开(公告)日：2007-07-26

    申请号：US11464361

    申请日：2006-08-14

    申请人： Xiaolian Gao ,  David Bear ,  George Church ,  Xiaochuan Zhou

    发明人： Xiaolian Gao ,  David Bear ,  George Church ,  Xiaochuan Zhou

    IPC分类号： A61K9/44 ,  C12Q1/68 ,  G01N33/551

    CPC分类号： G01N33/58 ,  G06Q10/08

    摘要： Compositions and methods for controlling and tracking items are provided. More particularly, labeling compositions useful as a coding system to deter diversion of items of medication or other valued items are provided, wherein the labeling compositions comprise at least 25 unique identifiers, wherein the at least 25 unique identifiers are selected from a set of at least 50 unique identifiers. Also provided are methods for using such compositions to provide trackability throughout a chain of custody.

    摘要翻译： 提供了用于控制和跟踪项目的组合和方法。 更具体地，提供了可用作编码系统的标记组合物，以阻止药物或其他重要物品的转移，其中所述标记组合物包含至少25个唯一标识符，其中所述至少25个唯一标识符选自至少一组 50个唯一标识符。 还提供了使用这种组合物以提供整个保管链的追踪性的方法。

    Accessible polynucleotide libraries and methods of use thereof
    17.
    发明申请
    Accessible polynucleotide libraries and methods of use thereof 审中-公开
    可接近的多核苷酸文库及其使用方法

    公开(公告)号：US20060281113A1

    公开(公告)日：2006-12-14

    申请号：US11436478

    申请日：2006-05-17

    申请人： George Church ,  Edmund Pitcher

    发明人： George Church ,  Edmund Pitcher

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12M1/34

    CPC分类号： C12N15/66 ,  C12N15/10 ,  C12N15/1093

    摘要： Disclosed are methods of assembling nucleic acid constructs from component parts in a manner that is not dependent on the sequence of the component parts. The methods may be used to assemble large nucleic acid constructs from multiple component parts in one or more reactions. The methods may also be used to assemble two or more nucleic acid constructs in the same reaction mixture. In exemplary embodiments, the methods involve formation of a Holliday junction or bridge structure using a junction oligonucleotide.

    摘要翻译： 公开了以不依赖于组分部分序列的方式从组分部分组装核酸构建体的方法。 该方法可用于在一个或多个反应中从多个组分部分组装大的核酸构建体。 该方法还可用于在相同的反应混合物中组装两个或多个核酸构建体。 在示例性实施方案中，所述方法包括使用连接寡核苷酸形成Holliday结或桥结构。

    Targeted polypeptide degradation
    18.
    发明申请
    Targeted polypeptide degradation 有权
    靶向多肽降解

    公开(公告)号：US20050152888A1

    公开(公告)日：2005-07-14

    申请号：US11003103

    申请日：2004-12-03

    申请人： George Church ,  Daniel Janse

    发明人： George Church ,  Daniel Janse

    IPC分类号： A61K38/48 ,  C12N9/64 ,  C12N15/62

    CPC分类号： C12N15/62 ,  C07K2319/20 ,  C07K2319/95 ,  G01N2500/02

    摘要： This invention pertains to compositions, methods, cells and organisms useful for selectively localizing polypeptides to the proteasome for degradation. Therapeutic methods and pharmaceutical compositions for treating disorders associated with the expression and/or activity of a polypeptide by targeting these polypeptides for degradation, as well as methods for targeting therapeutic polypeptides for degradation and/or activating therapeutic polypeptides by degradation are provided. The invention provides methods for identifying compounds that mediate proteasome localization and/or polypeptide degradation. The invention also provides research tools for the study of protein function.

    摘要翻译： 本发明涉及可用于将多肽选择性定位于蛋白酶体降解的组合物，方法，细胞和生物体。 提供了通过靶向这些用于降解的多肽来治疗与多肽的表达和/或活性相关的病症的治疗方法和药物组合物，以及用于通过降解降解和/或激活治疗性多肽的治疗性多肽的方法。 本发明提供鉴定介导蛋白酶体定位和/或多肽降解的化合物的方法。 本发明还提供了研究蛋白质功能的研究工具。

    METHODS AND CELLS FOR CREATING FUNCTIONAL DIVERSITY AND USES THEREOF
    20.
    发明申请
    METHODS AND CELLS FOR CREATING FUNCTIONAL DIVERSITY AND USES THEREOF 审中-公开
    用于创建功能多样性的方法和细胞及其用途

    公开(公告)号：US20090136986A1

    公开(公告)日：2009-05-28

    申请号：US12273098

    申请日：2008-11-18

    申请人： George Church ,  Brian M. Baynes

    发明人： George Church ,  Brian M. Baynes

    IPC分类号： C12Q1/02 ,  C12N15/74

    CPC分类号： C12N15/1027 ,  C12N15/1031

    摘要： The invention provides methods and compositions relating to cells having altered functions, and the nucleic acids that impart those functions. Altered cellular function arises from in vivo directed recombination of genetic elements to yield a recombined nucleic acid. These methods and compositions may utilize altered host cells having altered recombination enzyme profiles and/or altered recombination sites. The invention involves in some aspects methods for assembling nucleic acid molecules, such as genomic DNA. Aspects of the invention also provide kits, compositions, devices, and systems for generating novel recombined nucleic acids and cells having altered cell function.

    摘要翻译： 本发明提供了与具有改变功能的细胞相关的方法和组合物，以及赋予这些功能的核酸。 改变的细胞功能来源于遗传元件的体内靶向重组以产生重组的核酸。 这些方法和组合物可以利用具有改变的重组酶谱和/或改变的重组位点的改变的宿主细胞。 本发明涉及组装核酸分子（如基因组DNA）的方法。 本发明的方面还提供用于产生新的重组核酸和具有改变的细胞功能的细胞的试剂盒，组合物，装置和系统。