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    Methods for assembly of high fidelity synthetic polynucleotides
    1.
    发明申请
    Methods for assembly of high fidelity synthetic polynucleotides 审中-公开
    装配高保真合成多核苷酸的方法

    公开(公告)号：US20060194214A1

    公开(公告)日：2006-08-31

    申请号：US11068321

    申请日：2005-02-28

    申请人： George Church ,  Joseph Jacobson ,  Brian Baynes

    发明人： George Church ,  Joseph Jacobson ,  Brian Baynes

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/6813 ,  C12Q2523/305 ,  C12Q2525/131 ,  C12Q2525/155

    摘要： Disclosed are methods of manufacturing synthetic DNAs, that is, DNAs made at least in significant part by chemical synthesis of polynucleotide polymers. Also provided are methods for assembling plural DNAs in the same pool by multiplexed assembly of synthetic oligonucleotides. In exemplary embodiments, the methods involve pre-amplification of one or more oligonucleotides using “universal” primers, reduction of the error rate in oligonucleotide and/or polynucleotide products, and sequence optimization and oligonucleotides design.

    摘要翻译： 公开了制造合成DNA的方法，即通过化学合成多核苷酸聚合物至少部分制备的DNA。 还提供了通过合成寡核苷酸的多重组装在同一池中组装多个DNA的方法。 在示例性实施方案中，所述方法包括使用“通用”引物预扩增一个或多个寡核苷酸，降低寡核苷酸和/或多核苷酸产物中的错误率，以及序列优化和寡核苷酸设计。

    Methods for assembly of high fidelity synthetic polynucleotides
    3.
    发明申请
    Methods for assembly of high fidelity synthetic polynucleotides 审中-公开
    装配高保真合成多核苷酸的方法

    公开(公告)号：US20070122817A1

    公开(公告)日：2007-05-31

    申请号：US11067812

    申请日：2005-02-28

    申请人： George Church ,  Joseph Jacobson ,  Brian Baynes

    发明人： George Church ,  Joseph Jacobson ,  Brian Baynes

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6806 ,  C12Q1/6844 ,  C12Q2537/113 ,  C12Q2527/107 ,  C12Q2521/501

    摘要： Disclosed are methods of manufacturing synthetic DNAs, that is, DNAs made at least in significant part by chemical synthesis of polynucleotide polymers. Also provided are methods for assembling plural DNAs in the same pool by multiplexed assembly of synthetic oligonucleotides. In exemplary embodiments, the methods involve pre-amplification of one or more oligonucleotides using “universal” primers, reduction of the error rate in oligonucleotide and/or polynucleotide products, and sequence optimization and oligonucleotides design.

    摘要翻译： 公开了制造合成DNA的方法，即通过化学合成多核苷酸聚合物至少部分制备的DNA。 还提供了通过合成寡核苷酸的多重组装在同一池中组装多个DNA的方法。 在示例性实施方案中，所述方法包括使用“通用”引物预扩增一个或多个寡核苷酸，降低寡核苷酸和/或多核苷酸产物中的错误率，以及序列优化和寡核苷酸设计。

    Heirarchical assembly methods for genome engineering
    5.
    发明申请
    Heirarchical assembly methods for genome engineering 审中-公开
    基因工程的组织方法

    公开(公告)号：US20070004041A1

    公开(公告)日：2007-01-04

    申请号：US11331927

    申请日：2006-01-12

    申请人： George Church ,  Brian Baynes ,  Edmund Pitcher

    发明人： George Church ,  Brian Baynes ,  Edmund Pitcher

    IPC分类号： C12N15/74

    CPC分类号： C12N15/8201 ,  A01H1/06 ,  C12N15/102 ,  C12N15/1031 ,  C12N15/1058 ,  C12N15/1079 ,  C12N15/1082 ,  C12N15/66 ,  C12N15/67 ,  C12N15/8265 ,  C12N15/90

    摘要： The present invention provides recombination based methods for assembling nucleic acids. In certain aspects the present invention provides hierarchical assembly methods for producing genome sized polynucleotide constructs. The methods may be used for assembling large polynucleotide constructs, for synthesizing synthetic genomes, or for introducing a plurality of nucleotide changes throughout the genome of an organism. In another aspect, the invention provides cells having increased genomic stability. For example, cells comprising alterations in at least a substantial portion of the transposons in the genome are provided.

    摘要翻译： 本发明提供了用于组装核酸的基于重组的方法。 在某些方面，本发明提供了用于产生基因组大小的多核苷酸构建体的分层装配方法。 该方法可用于组装大型多核苷酸构建体，用于合成合成基因组，或用于在生物体的整个基因组中引入多个核苷酸变化。 另一方面，本发明提供具有增加的基因组稳定性的细胞。 例如，提供包含在基因组中的至少大部分转座子中的改变的细胞。

    Compositions and Methods for the Regulation of Multiple Genes of Interest in a Cell
    6.
    发明申请
    Compositions and Methods for the Regulation of Multiple Genes of Interest in a Cell 审中-公开
    用于调节细胞中多种感兴趣基因的组合物和方法

    公开(公告)号：US20120315670A1

    公开(公告)日：2012-12-13

    申请号：US13505376

    申请日：2010-11-02

    申请人： Joseph Jacobson ,  George Church

    发明人： Joseph Jacobson ,  George Church

    IPC分类号： C12P21/00 ,  C12N15/63

    CPC分类号： C12N15/1051 ,  C12N15/63

    摘要： Methods and compositions are provided for manipulating the genome of host cell to produce at least one exogenous gene product. Also provided are methods and composition for producing a programmable cell comprising a plurality of exogenous genes, wherein each exogenous gene is under the control of a disrupted regulatory sequence and wherein the disrupted regulatory sequences are restored by in vivo recombination. Preferably, the gene of interest is under the control of a genetically altered promoter which sequence recombination effects the expression of the exogenous gene(s).

    摘要翻译： 提供了用于操纵宿主细胞基因组以产生至少一种外源基因产物的方法和组合物。 还提供了用于生产包含多个外源基因的可编程细胞的方法和组合物，其中每个外源基因处于破坏的调节序列的控制下，并且其中破坏的调节序列通过体内重组恢复。 优选地，感兴趣的基因在遗传改变的启动子的控制下，该序列重组影响外源基因的表达。