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公开(公告)号：US20170204367A1
公开(公告)日：2017-07-20
申请号：US15394557
申请日：2016-12-29
Applicant: Massachusetts Institute of Technology
Inventor： Rohit Nandkumar Karnik , Seungpyo Hong , Ying Mei , Daniel Griffith Anderson , Jeffrey Michael Karp , Robert S. Langer , Suman Bose
CPC classification number: C12N5/0068 , B01L3/502715 , B01L3/502761 , B01L2200/0647 , B01L2300/0877 , B01L2300/16 , C12M23/16 , C12M47/02 , C12N5/0062 , C12N2501/58 , C12N2501/585 , C12N2533/50 , C12N2533/52 , C12N2535/10
Abstract: The present invention provides systems for cell separation based on cell rolling on surfaces along edges of regions coated with cell adhesion molecules. A variety of designs of coated regions and edges are disclosed.
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公开(公告)号：US09555413B2
公开(公告)日：2017-01-31
申请号：US14667615
申请日：2015-03-24
Applicant: Massachusetts Institute of Technology
Inventor： Rohit Nandkumar Karnik , Seungpyo Hong , Ying Mei , Daniel Griffith Anderson , Jeffrey Michael Karp , Robert S. Langer , Suman Bose
CPC classification number: C12N5/0068 , B01L3/502715 , B01L3/502761 , B01L2200/0647 , B01L2300/0877 , B01L2300/16 , C12M23/16 , C12M47/02 , C12N5/0062 , C12N2501/58 , C12N2501/585 , C12N2533/50 , C12N2533/52 , C12N2535/10
Abstract: The present invention provides systems for cell separation based on cell rolling on surfaces along edges of regions coated with cell adhesion molecules. A variety of designs of coated regions and edges are disclosed.
Abstract translation: 本发明提供了基于在细胞粘附分子包被的区域的边缘上的表面上的细胞滚动的细胞分离系统。 公开了涂覆区域和边缘的各种设计。
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公开(公告)号：US20210363491A1
公开(公告)日：2021-11-25
申请号：US17334933
申请日：2021-05-31
Inventor： Jeffrey Michael Karp , Robert Samuel Langer , Xiaolei Yin
Abstract: A population of enteroendocrine cells (EEC) is obtained from a mammalian post-natal cell population, such as a population including post-natal stem cells, by treating the population with a plurality of small molecules that upregulate ChgA and promote differentiation of the cells to form the enteroendocrine cells. The upregulation of ChgA is such that the fraction of cells expressing CGA in the obtained cell population, as measured by a ChgA Immunostaining Assay, is at least about 1.5%. Small molecules that can be used to differentiate the post-natal cells into the enteroendocrine cells can include at least one of a Wnt activator, a Notch inhibitor, a Wnt inhibitor, a MEK/ERK inhibitor, a growth factor, a HDAC inhibitor, a Histone Methylation Inhibitor, a Tgf-β inhibitor, and a NeuroD1 activator. Also, the insulin expression of a population of mammalian cells is increased by treating the population with a plurality of small molecules that increase the insulin expression.
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公开(公告)号：US20210301254A1
公开(公告)日：2021-09-30
申请号：US17208917
申请日：2021-03-22
Inventor： Jeffrey Michael Karp , Xiaolei Yin , Marc David Succi , Robert Samuel Langer
IPC: C12N5/071 , A61K31/19 , A61K31/506
Abstract: Described are cell culture solutions and systems for epithelial stem cell and organoid cultures, formation of epithelial constructs and uses of the same in transplantation.
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Patent Agency Ranking
公开(公告)号：US20200179050A1
公开(公告)日：2020-06-11
申请号：US16782029
申请日：2020-02-04
Applicant: President and Fellows of Harvard College , The Brigham and Women's Hospital , Children's Medical Center Corporation , Massachusetts Institute of Technology
Inventor： Conor J. Walsh , Ellen T. Roche , Panagiotis Polygerinos , Lucia R. Schuster , Jeffrey Michael Karp , Yuhan Lee , Pedro J. del Nido , Assunta Fabozzo , Ingeborg Friehs , Steven Charles Wasserman
Abstract: An insertable catheter device includes a shaft including a proximal end and a distal end, an expandable balloon, and an actuator configured to expand and retract the expandable balloon. The actuator includes a fluid conduit that extends through the shaft and is coupled with the expandable balloon to enable inflation and retraction of the expandable balloon via injection or withdrawal of a fluid to or from the expandable balloon via the fluid conduit. The expandable balloon is displaceably retractable into the shaft and extendable from the shaft. A fluid pump is coupled with the fluid conduit to pump the fluid through the fluid conduit. A patch is positioned to be displaced by the expandable balloon when the expandable balloon is inflated, and the expandable balloon is displaceably retractable into the shaft and displaceably extendable from the shaft.
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公开(公告)号：US10041046B2
公开(公告)日：2018-08-07
申请号：US14775560
申请日：2014-03-11
Inventor： Jeffrey Michael Karp , Xiaolei Yin , Marc David Succi , Robert Samuel Langer
IPC: C12N5/071
CPC classification number: C12N5/068 , A61K31/19 , A61K31/506 , C12N5/0679 , C12N2501/065 , C12N2501/11 , C12N2501/155 , C12N2501/415 , C12N2501/42 , C12N2501/727 , C12N2501/73 , C12N2513/00
Abstract: Described are cell culture solutions and systems for epithelial stem cell and organoid cultures, formation of epithelial constructs and uses of the same in transplantation. A single layer of epithelial cells that actively self-renews and is organized into crypts and villi clothes the intestine. It has been recently shown that the renewal of intestinal epithelium is driven by Lgr5+ intestinal stem cells (ISC) that reside at the base of these crypts (Barker et al., 2007). Lgr5+ stem cells can be isolated and cultured in vitro to form organoids containing crypt-vcllus structures that recapitulates the native intestinal epithelium (Sato et al., 2009).
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公开(公告)号：US20170349884A1
公开(公告)日：2017-12-07
申请号：US15400877
申请日：2017-01-06
Inventor： Jeffrey Michael Karp , Robert Samuel Langer , Xiaolei Yin
CPC classification number: C12N5/0678 , C12N5/0613 , C12N5/0679 , C12N2500/38 , C12N2500/62 , C12N2501/01 , C12N2501/065 , C12N2501/11 , C12N2501/119 , C12N2501/13 , C12N2501/15 , C12N2501/155 , C12N2501/335 , C12N2501/395 , C12N2501/415 , C12N2501/42 , C12N2501/71 , C12N2501/72 , C12N2501/727 , C12N2501/999 , C12N2502/23 , C12N2533/90 , G01N33/5008
Abstract: A population of enteroendocrine cells (EEC) is obtained from a mammalian post-natal cell population, such as a population including post-natal stem cells, by treating the population with a plurality of small molecules that upregulate ChgA and promote differentiation of the cells to form the enteroendocrine cells. The upregulation of ChgA is such that the fraction of cells expressing CGA in the obtained cell population, as measured by a ChgA Immunostaining Assay, is at least about 1.5%. Small molecules that can be used to differentiate the post-natal cells into the enteroendocrine cells can include at least one of a Wnt activator, a Notch inhibitor, a Wnt inhibitor, a MEK/ERK inhibitor, a growth factor, a HDAC inhibitor, a Histone Methylation Inhibitor, a Tgf-β inhibitor, and a NeuroD1 activator. Also, the insulin expression of a population of mammalian cells is increased by treating the population with a plurality of small molecules that increase the insulin expression.
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公开(公告)号：US20170226477A1
公开(公告)日：2017-08-10
申请号：US15494291
申请日：2017-04-21
Inventor： Jeffrey Michael Karp , Xiaolei Yin , Marc David Succi , Robert Samuel Langer
IPC: C12N5/071 , A61K31/19 , A61K31/506
CPC classification number: C12N5/068 , A61K31/19 , A61K31/506 , C12N5/0679 , C12N2501/065 , C12N2501/11 , C12N2501/155 , C12N2501/415 , C12N2501/42 , C12N2501/727 , C12N2501/73 , C12N2513/00
Abstract: Described are cell culture solutions and systems for epithelial stem cell and organoid cultures, formation of epithelial constructs and uses of the same in transplantation.
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公开(公告)号：US20150246354A1
公开(公告)日：2015-09-03
申请号：US14667615
申请日：2015-03-24
Applicant: Massachusetts Institute of Technology
Inventor： Rohit Nandkumar Karnik , Seungpyo Hong , Ying Mei , Daniel Griffith Anderson , Jeffrey Michael Karp , Robert S. Langer , Suman Bose
CPC classification number: C12N5/0068 , B01L3/502715 , B01L3/502761 , B01L2200/0647 , B01L2300/0877 , B01L2300/16 , C12M23/16 , C12M47/02 , C12N5/0062 , C12N2501/58 , C12N2501/585 , C12N2533/50 , C12N2533/52 , C12N2535/10
Abstract: The present invention provides systems for cell separation based on cell rolling on surfaces along edges of regions coated with cell adhesion molecules. A variety of designs of coated regions and edges are disclosed.
Abstract translation: 本发明提供了基于在细胞粘附分子包被的区域的边缘上的表面上的细胞滚动的细胞分离系统。 公开了涂覆区域和边缘的各种设计。
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