公开(公告)号：US20180245095A1

公开(公告)日：2018-08-30

申请号：US15732622

申请日：2017-12-05

Applicant: Monsanto Technology LLC

Inventor： Mark Scott Abad ,  Jeffrey E. Ahrens ,  Alice Clara Augustine ,  Erin Bell ,  Robert J. Bensen ,  Srikanth Venkatachalayya ,  Paolo Castiglioni ,  Richard Eric Cerny ,  Jaishree M. Chittoor-Vijayanath ,  Maureen Daley ,  Jill Deikman ,  Molian Deng ,  Todd DeZwaan ,  Stephen Duff ,  Michael D. Edgerton ,  Bradon J. Fabbri ,  Jason Fenner ,  Karen Gabbert ,  Garrett J. Lee ,  Barry S. Goldman ,  Deborah J. Hawkins ,  Steve He ,  Xianfeng Chen ,  Balasulojini Karunanandaa ,  Paul Loida ,  Savitha Madappa ,  Robert J. Meister ,  Ming Peng ,  Donald E. Nelson ,  Anand Pandravada ,  Obed Patty ,  Marie Petracek ,  Rajani Monnanda Somaiah ,  Monica P. Ravanello ,  Richard Johnson ,  Thomas G. Ruff ,  Rick A. Sanders ,  Beth Savidge ,  Steven H. Schwarts ,  Shobha Char ,  Padmini Sudarshana ,  Jindong Sun ,  Rebecca L. Thompson-Mize ,  Virginia Ursin ,  Dale L. Val ,  Tymagondlu V. Venkatesh ,  Kammaradi Vidya ,  K. Vijayalakshmi ,  Todd C. Weber ,  Jingrui Wu ,  Wei Zheng ,  Zhidong Xie ,  Dhanalaksmi Ramachandra

IPC: C12N15/82 ,  A01H1/02 ,  C12Q1/6895

Abstract: This invention provides recombinant DNA constructs, transgenic plant nuclei and cells with such recombinant DNA construct for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.

公开(公告)号：US20170067076A1

公开(公告)日：2017-03-09

申请号：US15348839

申请日：2016-11-10

Applicant: Monsanto Technology LLC

Inventor： Edwards Allen ,  William P. Donovan ,  Gregory R. Heck ,  James K. Roberts ,  Virginia Ursin ,  Yuanji Zhang

IPC: C12N15/82 ,  C12N15/113

Abstract: This invention provides plants having resistance to invertebrate pests. More specifically, this invention discloses a non-natural transgenic plant cell expressing at least one invertebrate miRNA in planta for suppression of a target gene of an invertebrate pest or of a symbiont associated with the invertebrate pest. Also provided are recombinant DNA constructs for expression of at least one invertebrate miRNA in planta, a non-natural transgenic plant containing the non-natural transgenic plant cell of this invention, a non-natural transgenic plant grown from the non-natural transgenic plant cell of this invention, and non-natural transgenic seed produced by the non-natural transgenic plants, as well as commodity products produced from a non-natural transgenic plant cell, plant, or seed of this invention. This invention further provides a method of suppressing at least one target gene of an invertebrate pest of a plant or of a symbiont associated with the invertebrate, including providing a plant including the non-natural transgenic plant cell of this invention, wherein the invertebrate is the invertebrate pest, the recombinant DNA is transcribed in the non-natural transgenic plant cell to the recombinant miRNA precursor, and when the invertebrate pest ingests the recombinant miRNA precursor, the at least one target gene is suppressed.

Abstract translation: 本发明提供具有抗无脊椎害虫的植物。 更具体地，本发明公开了一种非天然转基因植物细胞，其在植物中表达至少一种无脊椎动物miRNA，用于抑制无脊椎害虫或与无脊椎害虫相关的共生体的靶基因。 还提供了用于在植物中表达至少一种无脊椎动物miRNA的重组DNA构建体，包含本发明的非天然转基因植物细胞的非天然转基因植物，从非天然转基因植物细胞生长的非天然转基因植物 以及由非天然转基因植物产生的非天然转基因种子，以及由本发明的非天然转基因植物细胞，植物或种子产生的商品。 本发明还提供一种抑制植物无脊椎动物害虫或与无脊椎动物相关共生体的至少一种靶基因的方法，包括提供包含本发明非天然转基因植物细胞的植物，其中无脊椎动物是 无脊椎动物害虫，重组DNA在非天然转基因植物细胞中转录至重组miRNA前体，当无脊椎害虫摄入重组miRNA前体时，抑制至少一种靶基因。

公开(公告)号：US20150089683A1

公开(公告)日：2015-03-26

申请号：US14395636

申请日：2013-03-06

Applicant: Monsanto Technology LLC

Inventor： Mark Scott Abad ,  Pranesh Badami ,  Amarjit Basra ,  Erin Bell ,  Christopher P. Bonin ,  Paolo Castiglioni ,  Eric R. Cerny ,  Jaishree M. Chittoor-Vijayanath ,  Paul Chomet ,  Todd Dezwaan ,  Stephen Duff ,  Michael D. Edgerton ,  Barry S. Goldman ,  Balasulojini Karunanandaa ,  Kempagangaiah Krishnamurthy ,  Ping Li ,  Adrian A. Lund ,  Llinda Lutfiyya ,  Thomas M. Malvar ,  Donald E. Nelson ,  Monnanda S. Rajani ,  Dhanalakshmi Ramachandra ,  Thomas G. Ruff ,  Sara Salvador ,  S. Sangeetha ,  Char Shobha ,  Jeffrey M. Staub ,  Virginia Ursin ,  Tyamagondlu V. Venkatesh ,  Kammaradi R. Vidya ,  Xiaoyun Wu ,  Sean Yang ,  Jianmin Zhao

IPC: C12N15/82 ,  C12N5/04 ,  A01H1/02

CPC classification number: C12N15/8261 ,  A01H1/02 ,  C07K14/415 ,  C07K14/81 ,  C12N5/04 ,  C12N9/0006 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/825 ,  C12N15/8271 ,  C12N15/8273 ,  C12Y101/01049 ,  Y02A40/146

Abstract: This disclosure provides transgenic plants having enhanced traits such as increased yield, increased nitrogen use efficiency and enhanced drought tolerance; propagules, progeny and field crops of such transgenic plants; and methods of making and using such transgenic plants. This disclosure also provides methods of producing hybrid seed from such transgenic plants, growing such seed and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait.

Abstract translation: 本公开提供具有增强性状的转基因植物，例如增加的产量，增加的氮利用效率和增强的耐旱性; 这些转基因植物的繁殖体，后代和田间作物; 以及制备和使用这些转基因植物的方法。 本公开还提供了从这种转基因植物产生杂种种子的方法，种植这种种子并选择具有增强特征的后代植物。 还公开了具有改变的表型的转基因植物，其可用于筛选和选择所需增强性状的转基因事件。