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公开(公告)号：US4865979A
公开(公告)日：1989-09-12
申请号：US498802
申请日：1983-05-27
申请人： Eiichi Nakano , Tsutomu Masuda , Yasuji Koyama , Satoshi Kitao
发明人： Eiichi Nakano , Tsutomu Masuda , Yasuji Koyama , Satoshi Kitao
摘要： A bacteriophage which is characterized by the presence of one or more restriction enzyme sites downstream from the late promoter up to the cohesive end, and none or a permissible number of restriction enzyme sites upstream from the late promoter to the cohesive end is useful as an expression vector. The vector can be used to obtain high expression of DNA inserted at the restriction enzyme site downstream from the late promoter up to the cohesive end.
摘要翻译： 一种噬菌体，其特征在于存在一个或多个限制酶位点，从晚期启动子下游到粘性末端，并且没有或允许数量的限制酶位点从后期启动子上游到粘性末端可用作表达 向量。 该载体可用于获得插入在晚期启动子下游的限制酶位点直至粘性末端的DNA的高表达。
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公开(公告)号：US4348477A
公开(公告)日：1982-09-07
申请号：US87166
申请日：1979-10-22
申请人： Eiichi Nakano , Tsutomu Masuda , Narimasa Saito , Danji Fukushima
发明人： Eiichi Nakano , Tsutomu Masuda , Narimasa Saito , Danji Fukushima
CPC分类号： C12N15/00
摘要： A method for preparing a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a phage DNA having an endonuclease-sensitive region not in the DNA segment participating in temperate phage DNA replication and integration of DNA into a host chromosome but in other DNA segments, a temperate phage DNA having an endonuclease-sensitive region in the DNA segment carrying genetic information for the production of coat protein, and a DNA carrying the intended genetic information, (2) mixing together all fragments produced by said cleaving, (3) adding DNA ligase to the mixture, and (4) recovering from the resulting mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segment carrying genetic information for coat protein production with a DNA fragment carrying the intended genetic information.
摘要翻译： 一种制备新型重组DNA的方法，其包括（1）用核酸内切酶切割具有不在DNA片段中的内切核酸酶敏感区域的噬菌体DNA，其参与温和噬菌体DNA复制并将DNA整合到宿主染色体中，但在其他DNA （2）将由所述切割产生的所有片段混合在一起，（3）将所述切割产生的所有片段混合在一起，其中所述DNA片段具有用于产生外壳蛋白的遗传信息的DNA段中具有内切核酸酶敏感区的温带噬菌体DNA， 向混合物中加入DNA连接酶，（4）从所得混合物中回收具有外壳蛋白产生能力的噬菌体DNA，通过用携带预期遗传信息的DNA片段替代携带用于外壳蛋白生产的遗传信息的DNA片段而缺失。
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13.发明授权Variant E1 protein gene for pyruvate dehydrogenase complex and variant E1 protein of pyruvate dehydrogenase complex 失效丙酮酸脱氢酶复合物的变体E1蛋白基因和丙酮酸脱氢酶复合体的变体E1蛋白
公开(公告)号：US5432071A
公开(公告)日：1995-07-11
申请号：US215709
申请日：1994-03-22
申请人： Toshio Ichikawa , Yasuji Koyama , Hideko Otake , Eiichi Nakano
发明人： Toshio Ichikawa , Yasuji Koyama , Hideko Otake , Eiichi Nakano
CPC分类号： C12N9/0008 , C12Y102/04001 , C12Y102/05001
摘要： The present invention relates to variant E.sub.1 protein of pyruvate dehydrogenase complex of high activity in which arginine at the 146-position is replaced by proline in the amino acid sequence of wild-type E.sub.1 protein of pyruvate dehydrogenase complex, a gene coding for said variant E.sub.1 protein, a novel recombinant DNA comprising said variant E.sub.1 protein-encoding gene inserted in a vector DNA, and a process for producing variant E.sub.1 protein by said recombinant DNA. The present invention provides the variant E.sub.1 protein of pyruvate dehydrogenase complex of high activity.
摘要翻译： 本发明涉及高活性的丙酮酸脱氢酶复合物的变体E1蛋白，其中146位的精氨酸被丙酮酸脱氢酶复合物的野生型E1蛋白的氨基酸序列中的脯氨酸替代，编码所述变体E1的基因 蛋白质，包含插入载体DNA中的所述变体E1蛋白质编码基因的新型重组DNA，以及由所述重组DNA产生变体E1蛋白质的方法。 本发明提供了具有高活性的丙酮酸脱氢酶复合物的变体E1蛋白。
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公开(公告)号：US4501649A
公开(公告)日：1985-02-26
申请号：US548139
申请日：1983-11-02
申请人： Nobuo Furuno , Yoshio Ota , Masahiro Itai , Akio Tokuyama , Eiichi Nakano
发明人： Nobuo Furuno , Yoshio Ota , Masahiro Itai , Akio Tokuyama , Eiichi Nakano
IPC分类号： C25D13/24
CPC分类号： C25D13/24
摘要： The present invention provides a method of controlling an electrocoating bath using an ion exchange resin. The ion exchange resin is maintained in a porous container in a suspended state in the electrocoating bath, and is used in a quantity of not more than the chemical equivalent of excess counter-ion to be removed from the bath. Bath control is easily achieved without any coagulation trouble as often observed in conventional column methods.
摘要翻译： 本发明提供一种使用离子交换树脂控制电泳浴的方法。 离子交换树脂在电镀浴中以悬浮状态保持在多孔容器中，并且使用的量不超过要从浴中除去的过量抗衡离子的化学当量。 在常规柱方法中经常观察到的浴控制容易实现而没有任何凝结问题。
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15.发明授权DNA fragment containing a tannase gene, a recombinant plasmid, a process for producing tannase, and a promoter 失效含有鞣酸酶基因的DNA片段，重组质粒，鞣酸酶的制备方法和启动子
公开(公告)号：US5665584A
公开(公告)日：1997-09-09
申请号：US460860
申请日：1995-06-05
申请人： Osamu Hatamoto , Teruo Watarai , Kiyoshi Mizusawa , Eiichi Nakano
发明人： Osamu Hatamoto , Teruo Watarai , Kiyoshi Mizusawa , Eiichi Nakano
CPC分类号： C12N9/18
摘要： The present invention relates to a DNA fragment of 3,563 base pairs containing a gene coding for tannase and derived from a microorganism belonging to the genus Aspergillus, with the following restriction enzyme map: ##STR1## B: Bam HI, H: Hind III, K: Kpn I, S: Sal I, X: Xba I; a DNA fragment containing a tannase gene coding for the amino acid sequence of (SEQ ID NO:4); a recombinant plasmid comprising the DNA fragment containing the tannase gene inserted into a plasmid vector; a process for producing tannase, comprising culturing a microorganism belonging to the genus Aspergillus capable of producing tannase in medium with the recombinant plasmid, and recovering tannase from the culture; and a promoter represented by the nucleotide sequence of (SEQ ID NO:1). Tannase can be efficiently produced according to the present invention.
摘要翻译： 本发明涉及含有编码鞣酸酶的基因并衍生自属于曲霉属的微生物的3,563个碱基对的DNA片段，其具有以下限制酶图：B：Bam HI，H：Hind III，K ：Kpn I，S：Sal I，X：Xba I; 含有编码（SEQ ID NO：4）的氨基酸序列的鞣酸酶基因的DNA片段; 包含插入质粒载体中的含有鞣酸酶基因的DNA片段的重组质粒; 一种鞣酸酶的制造方法，其特征在于，在培养基中培养能够在培养基中生产鞣酸酶的微生物属于属于曲霉属的微生物，从培养物中回收鞣酸酶; 和由（SEQ ID NO：1）的核苷酸序列表示的启动子。 根据本发明可以有效地制备鞣酸酶。
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公开(公告)号：US5334224A
公开(公告)日：1994-08-02
申请号：US25841
申请日：1993-03-03
摘要： A method for the pretreatment of a long cloth continuously in which a cloth to be subjected to desizing, scouring and bleaching for the pretreatment thereof is preliminarily subjected to souring treatment in the sodium chlorite solution prepared by absorbing the chlorine dioxide gas, which is exhausted from the subsequent bleaching process with the use of chlorite, in a solution of H.sub.2 O.sub.2 and NaOH, and accordingly it is possible to transport the long cloth continuously in the pretreating process speedily with no need of staying the cloth in folded state for increasing the productivity and improving the quality of the product, and further to render the pretreating apparatus compact so as suitable for the production of small lot products.
摘要翻译： 一种连续预处理长纤维的方法，其中待进行退浆，精练和漂白处理的布预先在吸收二氧化氯气体制备的亚氯酸钠溶液中进行酸处理，该二氧化氯溶液是从 随后的使用亚氯酸盐的漂白工艺在H2O2和NaOH的溶液中，因此可以快速地在预处理过程中连续输送长布，而不需要将布留在折叠状态，以提高生产率和改善 产品的质量，进一步使预处理装置紧凑，从而适合生产小批量产品。
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公开(公告)号：US5180497A
公开(公告)日：1993-01-19
申请号：US757893
申请日：1991-09-11
CPC分类号： C02F9/00 , C02F1/26 , C02F1/285 , C02F1/54 , Y10S210/917
摘要： A method for the decolorization of waste water containing coloring matters comprising dyestuff and/or pigment, in which PVA is added to a waste water containing coloring matters as above mentioned so as to catch the coloring matters in the waste water into the PVA, a precipitant of the coloring matters is added to the resultant waste water so as to render the coloring matters absorbed onto the PVA insoluble, a coagulant of PVA is added to the waste water so as to make the PVA insoluble, and then the thus insolubilized PVA containing the coloring matters is separated for the recovery thereof, and thus the decolorization of a waste water containing the coloring matters can be done skillfully and economically by eliminating the drawbacks in the conventional arts.
摘要翻译： 一种含有染料和/或颜料的染色剂的废水脱色方法，其中将PVA加入到含有如上所述的着色剂的废水中，以将废水中的着色物质吸收到PVA中，沉淀剂 的着色剂加入到所得的废水中，以使着色物质吸收到PVA上不溶，将PVA的凝结剂加入到废水中，使PVA不溶，然后将含有 分离着色剂以使其回收，因此通过消除现有技术的缺点，可以巧妙地和经济地进行含有着色剂的废水的脱色。
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18.发明授权Method for separation of polyvinyl alcohol from aqueous solutions thereof using peroxodisulfates 失效使用过氧化脂肪酸从其水溶液中分离聚乙烯醇的方法
公开(公告)号：US5110481A
公开(公告)日：1992-05-05
申请号：US698670
申请日：1991-05-10
CPC分类号： C02F1/722 , C02F2101/34 , Y10S210/908
摘要： A method for separation of PVA from an aqueous solution thereof such as the waste water containing PVA from the pretreatment of a textile product, in which a peroxosulfate such as ammonium peroxosulfate is added to an aqueous PVA solution, particularly in an acidic condition with addition of sulfuric acid, at a temperature above 70.degree. C. for the separation of PVA, and as a result the thus separated PVA contains only a small amount of water in the range of about 60-70% and can easily be treated.
摘要翻译： 将PVA从其水溶液中分离的方法，例如含有PVA的废水从纺织产品的预处理中分离，其中将过硫酸盐如过硫酸铵加入到PVA水溶液中，特别是在酸性条件下，加入 硫酸，在70℃以上的温度下分离PVA，因此分离的PVA仅含有少量约60-70％范围内的水，并且可以容易地处理。
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19.发明授权
公开(公告)号：US4352277A
公开(公告)日：1982-10-05
申请号：US207778
申请日：1980-11-17
CPC分类号： D06B19/0094
摘要： An apparatus for continuously applying a treating solution as foam to a cloth, comprising a U-shaped foam tank for storing foam of a treating solution such as a dye solution supplied from an apparatus for generating foam of the treating solution, guide rolls for transporting a cloth to be treated continuously through the foam tank so as to adhere the foam of the treating solution to the cloth while the cloth is passing through the foam tank, and a means for controlling the amount of foam adhered to the cloth in taking out the cloth from the foam tank. By using this apparatus, a necessary and sufficient amount of the treating solution for the subsequent heat treatment of the cloth can be applied to the cloth uniformly with no need of removing the excess of treating solution and/or solvent from the cloth prior to the heat treatment.
摘要翻译： 一种用于将织物处理溶液连续地施加到布上的装置，包括用于储存由用于产生处理溶液的泡沫的装置供应的染色溶液等处理溶液的泡沫的U形泡沫罐，用于输送 布经由泡沫槽连续处理，以便在布通过泡沫罐时将处理溶液的泡沫粘附到布上;以及用于控制粘附到布上的泡沫量取出布的装置 从泡沫罐。 通过使用该装置，可以对织物进行后续热处理所需的和足够量的处理溶液均匀地施加到布上，而不需要在热量之前从布中除去过量的处理溶液和/或溶剂 治疗。
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公开(公告)号：US5407446A
公开(公告)日：1995-04-18
申请号：US132474
申请日：1993-10-06
CPC分类号： D06L3/04 , D06B21/00 , D06M10/001 , D06M10/025
摘要： A method and an apparatus for the continuous pretreatment of a long cloth produced commercially in completely gaseous system, in which, by utilizing the combination of a desizing and scouring process in a low temperature plasma atmosphere and a bleaching process in an atmosphere of ozone and ultraviolet ray radiation, and thus entirely no application of liquid system.
摘要翻译： 一种用于在完全气体系统中商业生产的长布的连续预处理的方法和装置，其中通过在低温等离子体气氛中利用脱浆和冲刷方法的组合以及在臭氧和紫外线气氛中的漂白工艺 射线辐射，因此完全没有液体系统的应用。
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