公开(公告)号：US07713532B2

公开(公告)日：2010-05-11

申请号：US10526744

申请日：2003-09-05

申请人： Noboru Maki ,  Tatsuji Kimura ,  Shintaro Yagi

发明人： Noboru Maki ,  Tatsuji Kimura ,  Shintaro Yagi

IPC分类号： A61K38/00

CPC分类号： C07K14/005 ,  A61K2039/5258 ,  C12N7/00 ,  C12N2730/10122 ,  C12N2730/10123 ,  G01N33/5761

摘要： There is provided a HBV precore protein having an ability of forming particles, and a means for determining it. A novel HBV precore protein that forms the virus (like) particles of HBV was identified. The present invention provides this novel HBV precore protein. Furthermore, there are provided core-like particles and virus-like particles formed by this HBV precore protein. These virus-like particles can be used for vaccines and therapeutic agents. The present invention also provides a method of determining the HBV precore protein and a method of determining the anti-HBV precore protein antibody.

摘要翻译： 提供了具有形成颗粒的能力的HBV前核蛋白，以及用于确定它的手段。 鉴定出形成HBV病毒（如）颗粒的新型HBV前核蛋白。 本发明提供了这种新型HBV前核蛋白。 此外，还提供了由该HBV前核蛋白形成的核心状颗粒和病毒样颗粒。 这些病毒样颗粒可用于疫苗和治疗剂。 本发明还提供了确定HBV前核蛋白的方法以及确定抗HBV前蛋白抗体的方法。

公开(公告)号：US20060166187A1

公开(公告)日：2006-07-27

申请号：US10526744

申请日：2003-09-05

申请人： Noboru Maki ,  Tatsuji Kimura ,  Shintaro Yagi

发明人： Noboru Maki ,  Tatsuji Kimura ,  Shintaro Yagi

IPC分类号： C12Q1/70 ,  C07K14/02

CPC分类号： C07K14/005 ,  A61K2039/5258 ,  C12N7/00 ,  C12N2730/10122 ,  C12N2730/10123 ,  G01N33/5761

摘要： There is provided a HBV precore protein having an ability of forming particles, and a means for determining it. A novel HBV precore protein that forms the virus (like) particles of HBV was identified. The present invention provides this novel HBV precore protein. Furthermore, there are provided core-like particles and virus-like particles formed by this HBV precore protein. These virus-like particles can be used for vaccines and therapeutic agents. The present invention also provides a method of determining the HBV precore protein and a method of determining the anti-HBV precore protein antibody.

摘要翻译： 提供了具有形成颗粒的能力的HBV前核蛋白，以及用于确定它的手段。 鉴定出形成HBV病毒（如）颗粒的新型HBV前核蛋白。 本发明提供了这种新型HBV前核蛋白。 此外，还提供了由该HBV前核蛋白形成的核心状颗粒和病毒样颗粒。 这些病毒样颗粒可用于疫苗和治疗剂。 本发明还提供了确定HBV前核蛋白的方法以及确定抗HBV前蛋白抗体的方法。

公开(公告)号：US5662906A

公开(公告)日：1997-09-02

申请号：US449093

申请日：1995-05-24

申请人： Noboru Maki ,  Kenjiro Yamaguchi ,  Ayumi Toyoshima ,  Michinori Kohara

发明人： Noboru Maki ,  Kenjiro Yamaguchi ,  Ayumi Toyoshima ,  Michinori Kohara

IPC分类号： C07K14/18 ,  C12N15/40 ,  A61K39/29

CPC分类号： C07K14/005 ,  C12N2770/24222

摘要： This invention relates to a DNA fragment comprising a base sequence encoding a non-A non-B hepatitis-specific antigen polypeptide, said base sequence being obtained using genetic engineering techniques from non-A non-B hepatitis virus RNA which is isolated directly from blood plasma from non-A non-B hepatitis patients, to an expression vector and a transformant for use in the expression of the DNA fragment, to a single strand DNA sequence for PCR primer, and to use of said polypeptide and said single strand DNA sequence in the detection of the non-A non-B hepatitis virus. The recombinant polypeptide and the single strand DNA sequence for PCR primer make it possible to detect the non-A non-B hepatitis virus with extremely high accuracy.

摘要翻译： 本发明涉及包含编码非A非乙型肝炎特异性抗原多肽的碱基序列的DNA片段，所述碱基序列使用从直接从血液中分离的非A非乙型肝炎病毒RNA的遗传工程技术获得 来自非A非B型肝炎患者的血浆，用于表达DNA片段的表达载体和转化体，用于PCR引物的单链DNA序列，以及使用所述多肽和所述单链DNA序列 在非A非乙型肝炎病毒的检测中。 用于PCR引物的重组多肽和单链DNA序列使得可以以非常高的精度检测非A非B型肝炎病毒。

公开(公告)号：US5416015A

公开(公告)日：1995-05-16

申请号：US89850

申请日：1993-07-09

申请人： Toshiya Hayano ,  Setsuko Katou ,  Noboru Maki ,  Nobuhiro Takahashi ,  Masanori Suzuki

发明人： Toshiya Hayano ,  Setsuko Katou ,  Noboru Maki ,  Nobuhiro Takahashi ,  Masanori Suzuki

IPC分类号： C12N9/90 ,  C12N15/61 ,  C12N15/63 ,  C12N15/70

CPC分类号： C12N9/90

摘要： A yeast PPIase characterized by possessing following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro stands for L-proline), (2) exhibiting a single molecular weight of about 17,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 6.2 in the isoelectric focusing, and (4) inhibited by CsA;an E. coli PPIase-.beta. characterized by possessing the following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro for L-proline), (2) exhibiting a single molecular weight of about 20,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 5.0 in the isoelectric focusing, and (4) no being inhibited by CsA;an E. coli PPIase-.alpha. characterized by possessing the following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro for L-proline), (2) exhibiting a single molecular weight of about 22,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 9.7 in the isoelectric focusing, and (4) not being inhibited by CsA.

摘要翻译： 特征在于具有以下性质的酵母PPI酶：（1）作用于Xaa-Pro键（Xaa代表任何氨基酸，Pro代表L-脯氨酸），（2）表现出约17,000道尔顿单分子量 在十二烷基硫酸钠 - 聚丙烯酰胺浓度梯度凝胶电泳中，（3）在等电聚焦中表现出约6.2的单一等电点，（4）被CsA抑制; 特征在于具有以下性质的大肠杆菌PPI酶-β：（1）作用于Xaa-Pro（Xaa代表任何氨基酸，Pro-L-脯氨酸，Xaa代表任意氨基酸，Pro表示单分子量） 在十二烷基硫酸钠 - 聚丙烯酰胺浓度梯度凝胶电泳中为约20,000道尔顿，（3）在等电聚焦中呈现约5.0的单等电点，（4）不被CsA抑制; 其特征在于具有以下性质：（1）作用于Xaa-Pro键（Xaa代表任何氨基酸，Pro为L-脯氨酸），（2）表现出单分子量 在十二烷基硫酸钠 - 聚丙烯酰胺浓度梯度凝胶电泳中约为22,000道尔顿，（3）在等电聚焦中显示出约9.7的单一等电点，（4）不被CsA抑制。

公开(公告)号：US08981073B2

公开(公告)日：2015-03-17

申请号：US13823422

申请日：2011-10-07

申请人： Kenichi Mori ,  Noboru Maki ,  Hiromi Fukai

发明人： Kenichi Mori ,  Noboru Maki ,  Hiromi Fukai

IPC分类号： C07H21/04 ,  A01N63/02 ,  C12N7/00 ,  C07H21/02 ,  C07K14/005

CPC分类号： C07H21/02 ,  C07K14/005 ,  C12N2770/24222

摘要： Disclosed are an HCV gene having higher replication efficiency and higher reinfection efficiency than the known HCV gene of genotype 1b, an RNA replicon having this gene, a cell infected with this RNA replicon, which cell allows replication of HCV, and an HCV particle. The hepatitis C virus gene encodes an amino acid sequence wherein the 979th amino acid is threonine; the 1804th amino acid is leucine; and the 1966th amino acid is lysine. An HCV gene which can propagate in vitro and has higher replication efficiency and higher reinfection efficiency than the known HCV gene of genotype 1b was provided.

摘要翻译： 公开了具有比基因型1b的已知HCV基因，具有该基因的RNA复制子，感染了该RNA复制子的细胞，该细胞允许HCV复制和HCV颗粒更高的复制效率和更高的再感染效率的HCV基因。 丙型肝炎病毒基因编码氨基酸序列，其中第979位氨基酸是苏氨酸; 第1804位氨基酸为亮氨酸; 而第1966位氨基酸是赖氨酸。 提供了可以在体外繁殖并且具有比已知的基因型1b的HCV基因更高的复制效率和更高的再感染效率的HCV基因。

公开(公告)号：US08741607B2

公开(公告)日：2014-06-03

申请号：US13054356

申请日：2009-07-15

申请人： Noboru Maki ,  Kenichi Mori ,  Hiromi Fukai

发明人： Noboru Maki ,  Kenichi Mori ,  Hiromi Fukai

IPC分类号： C12N15/09 ,  C12N5/10 ,  C12N7/00 ,  C07K14/18

CPC分类号： C07K14/005 ,  C12N7/00 ,  C12N2770/24221 ,  C12N2770/24222 ,  C12N2770/24243

摘要： An HCV/GBV-B chimeric virus which maintains the replication function of HCV and is capable of infecting tamarin is disclosed in order to construct an HCV animal model which can be used as a development or evaluation system for therapeutic agents for HCV. The HCV/GBV-B chimeric RNA comprises an RNA of hepatitis C virus and an RNA of GB virus-B, wherein the RNA of hepatitis C virus comprises an RNA encoding leucine at the 1804th position and lysine at the 1966th position in the amino acid sequence of the polyprotein of hepatitis C virus.

摘要翻译： 公开了保持HCV的复制功能并能够感染t am素的HCV / GBV-B嵌合病毒，以构建可用作HCV治疗剂的开发或评估系统的HCV动物模型。 HCV / GBV-B嵌合RNA包含丙型肝炎病毒的RNA和GB病毒B的RNA，其中丙型肝炎病毒的RNA包含编码第1804位亮氨酸的RNA，氨基酸中第1966位的赖氨酸 丙型肝炎病毒多蛋白序列。

公开(公告)号：US20130189779A1

公开(公告)日：2013-07-25

申请号：US13823422

申请日：2011-10-07

申请人： Kenichi Mori ,  Noboru Maki ,  Hiromi Fukai

发明人： Kenichi Mori ,  Noboru Maki ,  Hiromi Fukai

IPC分类号： C07H21/02

CPC分类号： C07H21/02 ,  C07K14/005 ,  C12N2770/24222

摘要： Disclosed are an HCV gene having higher replication efficiency and higher reinfection efficiency than the known HCV gene of genotype 1b, an RNA replicon having this gene, a cell infected with this RNA replicon, which cell allows replication of HCV, and an HCV particle. The hepatitis C virus gene encodes an amino acid sequence wherein the 979th amino acid is threonine; the 1804th amino acid is leucine; and the 1966th amino acid is lysine. An HCV gene which can propagate in vitro and has higher replication efficiency and higher reinfection efficiency than the known HCV gene of genotype 1b was provided.

摘要翻译： 公开了具有比基因型1b的已知HCV基因，具有该基因的RNA复制子，感染了该RNA复制子的细胞，该细胞允许HCV复制和HCV颗粒更高的复制效率和更高的再感染效率的HCV基因。 丙型肝炎病毒基因编码氨基酸序列，其中第979位氨基酸是苏氨酸; 第1804位氨基酸为亮氨酸; 而第1966位氨基酸是赖氨酸。 提供了可以在体外繁殖并且具有比已知的基因型1b的HCV基因更高的复制效率和更高的再感染效率的HCV基因。

公开(公告)号：US5641654A

公开(公告)日：1997-06-24

申请号：US081072

申请日：1993-06-22

申请人： Noboru Maki ,  Kenjiro Yamaguchi ,  Ayumi Toyoshima ,  Michinori Kohara

发明人： Noboru Maki ,  Kenjiro Yamaguchi ,  Ayumi Toyoshima ,  Michinori Kohara

IPC分类号： C07K14/18 ,  C12N15/40 ,  C12P21/02 ,  C12N1/21 ,  C12N15/71

CPC分类号： C07K14/005 ,  C12N2770/24222

摘要： This invention relates to a DNA fragment comprising a base sequence encoding a non-A non-B hepatitis-specific antigen polypeptide, said base sequence being obtained using genetic engineering techniques from non-A non-B hepatitis virus RNA which is isolated directly from blood plasma from non-A non-B hepatitis patients, to an expression vector and a transformant for use in the expression of the DNA fragment, to a single strand DNA sequence for PCR primer, and to use of said polypeptide and said single strand DNA sequence in the detection of the non-A non-B hepatitis virus. The recombinant polypeptide and the single strand DNA sequence for PCR primer make it possible to detect the non-A non-B hepatitis virus with extremely high accuracy.

摘要翻译： 本发明涉及包含编码非A非乙型肝炎特异性抗原多肽的碱基序列的DNA片段，所述碱基序列使用从直接从血液中分离的非A非乙型肝炎病毒RNA的遗传工程技术获得 来自非A非B型肝炎患者的血浆，用于表达DNA片段的表达载体和转化体，用于PCR引物的单链DNA序列，以及使用所述多肽和所述单链DNA序列 在非A非乙型肝炎病毒的检测中。 用于PCR引物的重组多肽和单链DNA序列使得可以以非常高的精度检测非A非B型肝炎病毒。

公开(公告)号：US5284762A

公开(公告)日：1994-02-08

申请号：US555944

申请日：1990-07-19

申请人： Toshiya Hayano ,  Setsuko Katou ,  Noboru Maki ,  Nobuhiro Takahashi ,  Masanori Suzuki

发明人： Toshiya Hayano ,  Setsuko Katou ,  Noboru Maki ,  Nobuhiro Takahashi ,  Masanori Suzuki

IPC分类号： C12N9/90 ,  C12N15/61 ,  C12N15/70

CPC分类号： C12N9/90

摘要： A yeast PPIase characterized by possessing the following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro stands for L-proline), (2) exhibiting a single molecular weight of about 17,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 6.2 in the isoelectric focusing, and (4) inhibited by CsA;an E. coli PPIase-.beta. characterized by possessing the following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro for L-proline), (2) exhibiting a single molecular weight of about 20,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 5.0 in the isoelectric focusing, and (4) no being inhibited by CsA;an E. coli PPIase-.alpha. characterized by possessing the following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro for L-proline), (2) exhibiting a single molecular weight of about 22,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 9.7 in the isoelectric focusing, and (4) not being inhibited by CsA.

摘要翻译： 特征在于具有以下特性的酵母PPI酶：（1）作用于Xaa-Pro键（Xaa代表任何氨基酸，Pro代表L-脯氨酸），（2）表现出单一分子量约17,000 十二烷基硫酸钠 - 聚丙烯酰胺浓度梯度凝胶电泳中的道尔顿，（3）在等电聚焦中显示约6.2的单一等电点，和（4）被CsA抑制; 特征在于具有以下特性的大肠杆菌PPIase-（β）：（1）作用于Xaa-Pro键（Xaa代表任何氨基酸，Pro代表L-脯氨酸），（2）表现出单一的 在十二烷基硫酸钠 - 聚丙烯酰胺浓度梯度凝胶电泳中分子量约20,000道尔顿，（3）在等电聚焦中呈现约5.0的单一等电点，（4）不被CsA抑制; 特征在于具有以下性质的大肠杆菌PPIase-（α）：（1）作用于Xaa-Pro键（Xaa代表任何氨基酸，Pro代表L-脯氨酸），（2）表现出单一的 在十二烷基硫酸钠 - 聚丙烯酰胺浓度梯度凝胶电泳中分子量约22,000道尔顿，（3）在等电聚焦中显示约9.7的单一等电点，（4）不被CsA抑制。

公开(公告)号：US5759802A

公开(公告)日：1998-06-02

申请号：US417429

申请日：1989-10-05

申请人： Noboru Maki ,  Shintaro Yagi ,  Kazuya Watanabe ,  Masanori Suzuki

发明人： Noboru Maki ,  Shintaro Yagi ,  Kazuya Watanabe ,  Masanori Suzuki

IPC分类号： C07K14/765 ,  C12N1/19 ,  C12N15/14 ,  C12N15/62 ,  C12N15/81 ,  C12P21/06 ,  C12N1/14 ,  C12N15/00 ,  C12P21/04

CPC分类号： C07K14/765 ,  C12N15/625 ,  C12N15/81 ,  C07K2319/02 ,  C07K2319/036

摘要： A process for the production of human serum albumin A (HSA) characterized by culturing host cells transformed with the plasmids containing a leader DNA sequence and a cDNA coding for mature HSA to produce and secrete mature HSA, and recovering the mature HSA. As the leader HSA sequence, a cDNA coding for the prepro sequence of HSA, a synthetic DNA coding for the prepro sequence of HSA, by the codons frequently used in a selected host, a DNA coding for a chimeric leader peptide, a DNA coding for MF.alpha.1 prepro sequence, or a DNA coding for PH05 signal peptide, is used.

摘要翻译： 一种生产人血清白蛋白A（HSA）的方法，其特征在于培养用含有前导DNA序列的质粒转化的宿主细胞和编码成熟HSA的cDNA，以产生和分泌成熟HSA，并回收成熟HSA。 作为前导HSA序列，编码HSA的前序列的cDNA，编码HSA的前序列的合成的DNA，经选择的宿主中常用的密码子，编码嵌合前导肽的DNA，编码 使用MFα1前原序列或编码PH05信号肽的DNA。