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    ALPHA-AMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME
    11.
    发明公开
    ALPHA-AMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME 审中-公开

    公开(公告)号：US20230142991A1

    公开(公告)日：2023-05-11

    申请号：US17797351

    申请日：2021-02-09

    Applicant: Novozymes A/S

    Inventor： Carsten Andersen ,  Thomas Agersten Poulsen ,  Dorota Nissen ,  Marie Thryøse Kruse ,  Sarah Schultheis Elliott ,  Shiro Fukuyama

    IPC: C12N9/28 ,  C12N9/34 ,  C12P7/06

    CPC classification number: C12N9/2417 ,  C12Y302/01001 ,  C12N9/2428 ,  C12P7/06

    Abstract: The present invention relates to isolated alpha-amylase variants of a parent alpha-amylase, comprising an alteration at one or more positions corresponding to positions 196, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 28, 38, 39, 43, 54, 56, 57, 64, 67, 68, 70, 71, 86, 89, 90, 94, 96, 99, 101, 103, 107, 108, 110, 113, 114, 117, 127, 134, 138, 142, 150, 151, 152, 156, 169, 171, 174, 179, 183, 193, 199, 200, 204, 205, 207, 208, 209, 212, 218, 221, 222, 224, 233, 241, 245, 259, 275, 278, 281, 282, 283, 284, 285, 308, 323, 335, 348, 359, 382, 386, 388, 392, 394, 396, 412, 414, 417, 424, 428, 457, 459, 466, 479, 489, 511, 533, 534, 542, 543, 545, 547, 549, 550, 551, 560, 566, 570, 574, 575, 576, 577, 578, 580, 581, 582, 589, 592, 599, 603, 605, 608, 614, 619, or 626 of the polypeptide of SEQ ID NO: 1, wherein the variant has alpha-amylase activity and wherein the variant has at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at 1.5 least 98%, or at least 99% sequence identity, but less than 100% sequence identity, to the polypeptide of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4. The alpha-amylase variants of the invention have increased stability at pH 4.0 and 32° C. compared to the parent alpha-amylase.

    Compositions for Producing Glucose Syrups
    12.
    发明申请
    Compositions for Producing Glucose Syrups 有权

    公开(公告)号：US20210395791A1

    公开(公告)日：2021-12-23

    申请号：US17465098

    申请日：2021-09-02

    Applicant: Novozymes A/S

    Inventor： Shiro Fukuyama ,  Keiichi Ayabe

    IPC: C12P19/14 ,  A23L29/30 ,  C12N9/24 ,  C12N9/26 ,  C12N9/44 ,  C12P19/02 ,  C13K1/06 ,  C12N9/34

    Abstract: The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.

    Compositions for producing glucose syrups
    13.
    发明授权
    Compositions for producing glucose syrups 有权

    公开(公告)号：US11136607B2

    公开(公告)日：2021-10-05

    申请号：US16403919

    申请日：2019-05-06

    Applicant: Novozymes A/S

    Inventor： Shiro Fukuyama ,  Keiichi Ayabe

    IPC: C12P19/14 ,  A23L29/30 ,  C12N9/24 ,  C12N9/26 ,  C12N9/44 ,  C12P19/02 ,  C13K1/06 ,  C12N9/34

    Abstract: The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.

    Compositions for producing glucose syrups
    18.
    发明授权
    Compositions for producing glucose syrups 有权

    公开(公告)号：US10337041B2

    公开(公告)日：2019-07-02

    申请号：US15116006

    申请日：2015-02-06

    Applicant: Novozymes A/S

    Inventor： Shiro Fukuyama ,  Keiichi Ayabe

    IPC: C12P19/14 ,  C12N9/34 ,  C12P19/02 ,  C12N9/44 ,  C12N9/24 ,  A23L29/30 ,  C13K1/06 ,  C12N9/26

    Abstract: The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.

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