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19 条记录 (耗时 0.058 秒)

    Rhizobial diagnostic probes and rhizobium trifolii nifH promoters
    11.
    发明授权
    Rhizobial diagnostic probes and rhizobium trifolii nifH promoters 失效
    根瘤菌诊断探针和根瘤菌trifolii nifH启动子

    公开(公告)号：US5137816A

    公开(公告)日：1992-08-11

    申请号：US701358

    申请日：1991-05-10

    申请人： Barry G. Rolfe ,  John Shine ,  Kieran F. Scott ,  John M. Watson ,  Peter Schofield

    发明人： Barry G. Rolfe ,  John Shine ,  Kieran F. Scott ,  John M. Watson ,  Peter Schofield

    IPC分类号： C07K14/195 ,  C07K14/325 ,  C07K14/825 ,  C12N9/02 ,  C12N15/11 ,  C12N15/74 ,  C12N15/90 ,  C12Q1/68

    CPC分类号： C12N9/0095 ,  C07K14/195 ,  C07K14/325 ,  C07K14/825 ,  C12N15/11 ,  C12N15/743 ,  C12N15/90 ,  C12Q1/689 ,  C12Y118/06001 ,  Y10S435/878

    摘要： This invention provides useful promoters from the R. trifolii nifH gene for the construction of recombinant molecules to regulate foreign genes for expression under desired conditions. In particular, the promoters act to control expression of the foreign genes within root nodules formed by rhizobial bacterial strains in symbiotic combination with host plants.A rhizobium diagnostic segment (RDS) is also provided comprising a DNA segment found at more than one location in rhizobia, the RDS being species-specific, and detectable by DNA hybridization under stringent conditions. A recombinant plasmid comprising a RDS and a bacterial strain containing the plasmid are provided. Methods are provided for identifying species and strains of field isolates of Rhizobium, using RDS's. One RDS exemplified comprises 5' sequences from the R. trifolii nifH gene.

    摘要翻译： 本发明提供了来自三叶草nifH基因的有用的启动子，用于构建重组分子以调节外来基因以在期望的条件下表达。 特别地，启动子用于控制与宿主植物共生组合的由根瘤菌细菌菌株形成的根结节内的外源基因的表达。 还提供了根瘤菌诊断片段（RDS），其包含在根瘤菌中多于一个位置处发现的DNA片段，RDS是物种特异性的，并且可以在严格条件下通过DNA杂交检测。 提供了包含RDS和含有该质粒的细菌菌株的重组质粒。 提供了使用RDS来鉴定根瘤菌的分离株的种类和菌株的方法。 一个RDS示例包括来自三叶草nifH基因的5'序列。

    Purification of nucleotide sequences suitable for expression in bacteria
    14.
    发明授权
    Purification of nucleotide sequences suitable for expression in bacteria 失效
    纯化适合在细菌中表达的核苷酸序列

    公开(公告)号：US4283489A

    公开(公告)日：1981-08-11

    申请号：US97049

    申请日：1979-11-23

    申请人： Howard M. Goodman ,  John Shine ,  Peter Horst

    发明人： Howard M. Goodman ,  John Shine ,  Peter Horst

    IPC分类号： C07K14/575 ,  C12N15/10

    CPC分类号： C12N15/1003 ,  C07K14/57518 ,  C12N15/1096

    摘要： A method has been discovered for purifying a specific desired DNA sequence, starting from RNA heterogeneous in length and sequence. The steps of the method include making complementary DNA transcripts of the RNA by means of an enzyme such as reverse transcriptase, subjecting the DNA transcripts to the action of one or more selected restriction endonuclease enzymes, and fractionating the fragments produced by endonuclease action according to their length. By this method it is possible to isolate homogeneous length DNA fragments complementary to RNA sequences present in the original preparation in as low a frequency as two percent. A method is also disclosed for further purifying the homogeneous length fragments and for determining their final purity. Using the disclosed methods, a DNA fragment approximately 550 nucleotides in length coding for a portion of the peptide hormone, human chorionic somatomammotropin, has been purified to greater than 99% purity.

    摘要翻译： 已经发现了从长度和序列异质性起源的纯化特定的所需DNA序列的方法。 该方法的步骤包括通过酶如逆转录酶制备RNA的互补DNA转录物，使DNA转录物受到一种或多种选择的限制性内切核酸酶的作用，并且根据其内切核酸酶作用产生的片段进行分级 长度。 通过该方法，可以将原始制剂中存在的RNA序列互补的均质长度的DNA片段以2％的频率分离。 还公开了进一步纯化均匀长度片段并确定其最终纯度的方法。 使用所公开的方法，编码一部分肽激素（人绒毛膜促生长激素）的大约550个核苷酸长度的DNA片段已被纯化至大于99％的纯度。

    DNA Joining method
    15.
    发明授权
    DNA Joining method 失效
    DNA连接法

    公开(公告)号：US4264731A

    公开(公告)日：1981-04-28

    申请号：US898887

    申请日：1978-04-21

    申请人： John Shine

    发明人： John Shine

    IPC分类号： C07K14/62 ,  C12N15/00 ,  C12P19/34 ,  C12R1/19

    CPC分类号： C07K14/62 ,  C12N15/00 ,  Y10S435/849

    摘要： A selected portion of DNA molecules having reactant ends which are capable of being joined in a ligase catalyzed reaction are pretreated so a to remove the 5'-terminal phosphate groups. Such a treatment reduces the frequency of joining an undersired combination and enhances the frequency of joining the desired combination.

    摘要翻译： 在连接酶催化反应中能够连接的具有反应物末端的DNA分子的选定部分进行预处理，以除去5'-末端磷酸酯基团。 这种处理降低了连接较低组合的频率，并提高了连接所需组合的频率。

    Adrenocorticotropin-lipotropin precursor gene
    19.
    发明授权
    Adrenocorticotropin-lipotropin precursor gene 失效
    促肾上腺皮质激素 - 促脂分子前体基因

    公开(公告)号：US4322499A

    公开(公告)日：1982-03-30

    申请号：US972430

    申请日：1978-12-22

    申请人： John D. Baxter ,  James L. Roberts ,  Peter H. Seeburg ,  Howard M. Goodman ,  John Shine

    发明人： John D. Baxter ,  James L. Roberts ,  Peter H. Seeburg ,  Howard M. Goodman ,  John Shine

    IPC分类号： C07K14/665 ,  C12N1/00

    CPC分类号： C07K14/665

    摘要： A technique suitable for cloning a cDNA having a base sequence coding for the ACTH/LPH precursor is disclosed. The invention is exemplified by the cloning of a cDNA fragment comprising a base sequence coding for the endorphin region. The fragment, hereinafter termed the endorphin gene cDNA sequence, was obtained from cultured mouse pituitary tumor cells known to produce the ACTH/LPH precursor protein.

    摘要翻译： 公开了一种适用于克隆编码ACTH / LPH前体碱基序列的cDNA的技术。 通过克隆包含编码内啡肽区域的碱基序列的cDNA片段来举例说明本发明。 该片段（以下称为内啡肽基因cDNA序列）从已知产生ACTH / LPH前体蛋白的培养的小鼠垂体肿瘤细胞获得。