公开(公告)号：US12174140B2

公开(公告)日：2024-12-24

申请号：US16760723

申请日：2018-10-30

Applicant: President and Fellows of Harvard College

Inventor： Donhee Ham ,  Hongkun Park ,  Keith Krenek ,  Tianyang Ye ,  Jeffrey T. Abbott ,  Wenxuan Wu

IPC: G01N27/327 ,  C12M1/34 ,  C12N13/00 ,  G01N27/416

Abstract: Methods and systems for monitoring the activity of electrogenic networks are described. One representative system includes an array of electrode coupled to an analyzer having a stimulator and a receiver. The electrode is placed in contact with an electrogenic cell. The electrodes can be shaped as nanowires, tubes, cavities and/or cones. The analyzer may be configured to operate in a voltage stimulation mode, in which the cells are stimulated via voltages and monitored via current, or in a current stimulation mode, in which the cells are stimulated via currents and monitored via voltages. The analyzers may be arranged as single-stage amplifiers, and may include a feedback loop shared between the stimulation signal path and the sensing signal path. The feedback loop may be arranged to provide overlapping stimulation and sensing of the electrogenic network's cells.

公开(公告)号：US11774396B2

公开(公告)日：2023-10-03

申请号：US17940174

申请日：2022-09-08

Applicant: President and Fellows of Harvard College

Inventor： Hongkun Park ,  Jeffrey T. Abbott ,  Wenxuan Wu ,  Tianyang Ye ,  Han Sae Jung ,  Donhee Ham

IPC: G01N27/327 ,  B01J19/08 ,  C12Q1/02 ,  G01N33/483

CPC classification number: G01N27/3277 ,  B01J19/087 ,  C12Q1/02 ,  G01N33/4836

Abstract: Disclosed herein are an apparatus for electrically assessing and/or manipulating cells. One aspect is directed to electrically mapping cells on the surface of the semiconductor substrate via cross-electrode impedance measurements. Further according to some aspects, the electrode array allows for spatially addressable electrical stimulation and/or recording of electrical signals in real-time using the CMOS circuitry. Some of these aspects are directed to using an electrode array to perform cell patterning through electrochemical gas generation, and extracellular electrochemical mapping.

公开(公告)号：US20210187280A1

公开(公告)日：2021-06-24

申请号：US17144387

申请日：2021-01-08

Applicant: President and Fellows of Harvard College

Inventor： Hongkun Park ,  Donhee Ham ,  Jeffrey T. Abbott ,  Ling Qin ,  Marsela Jorgolli ,  Tianyang Ye

IPC: A61N1/05 ,  H01L23/48 ,  H01L29/06 ,  A61N1/06 ,  B82Y5/00 ,  H01L23/49

Abstract: The present invention generally relates to nanowires. In one aspect, the present invention is generally directed to systems and methods of individually addressing nanowires on a surface, e.g., that are substantially upstanding or vertically-oriented with respect to the surface. In some cases, one or more nanowires may be individually addressed using various integrated circuit (“IC”) technologies, such as CMOS. For example, the nanowires may form an array on top of an active CMOs integrated circuit.

公开(公告)号：US20240255461A1

公开(公告)日：2024-08-01

申请号：US18240894

申请日：2023-08-31

Applicant: President and Fellows of Harvard College

Inventor： Hongkun Park ,  Jeffrey T. Abbott ,  Wenxuan Wu ,  Tianyang Ye ,  Han Sae Jung ,  Donhee Ham

IPC: G01N27/327 ,  B01J19/08 ,  C12Q1/02 ,  G01N33/483

CPC classification number: G01N27/3277 ,  B01J19/087 ,  C12Q1/02 ,  G01N33/4836

Abstract: Disclosed herein are an apparatus for electrically assessing and/or manipulating cells. One aspect is directed to electrically mapping cells on the surface of the semiconductor substrate via cross-electrode impedance measurements. Further according to some aspects, the electrode array allows for spatially addressable electrical stimulation and/or recording of electrical signals in real-time using the CMOS circuitry. Some of these aspects are directed to using an electrode array to perform cell patterning through electrochemical gas generation, and extracellular electrochemical mapping.

公开(公告)号：US11833346B2

公开(公告)日：2023-12-05

申请号：US17144387

申请日：2021-01-08

Applicant: President and Fellows of Harvard College

Inventor： Hongkun Park ,  Donhee Ham ,  Jeffrey T. Abbott ,  Ling Qin ,  Marsela Jorgolli ,  Tianyang Ye

IPC: A61N1/05 ,  H01L23/48 ,  H01L29/06 ,  A61N1/06 ,  B82Y5/00 ,  H01L23/49

CPC classification number: A61N1/0531 ,  A61N1/05 ,  A61N1/0536 ,  A61N1/06 ,  B82Y5/00 ,  H01L23/48 ,  H01L23/49 ,  H01L29/0676 ,  H01L2924/0002 ,  H01L2924/10253 ,  H01L2924/14 ,  H01L2924/0002 ,  H01L2924/00

Abstract: The present invention generally relates to nanowires. In one aspect, the present invention is generally directed to systems and methods of individually addressing nanowires on a surface, e.g., that are substantially upstanding or vertically-oriented with respect to the surface. In some cases, one or more nanowires may be individually addressed using various integrated circuit (“IC”) technologies, such as CMOS. For example, the nanowires may form an array on top of an active CMOs integrated circuit.

公开(公告)号：US11768196B2

公开(公告)日：2023-09-26

申请号：US16625603

申请日：2018-07-06

Applicant: President and Fellows of Harvard College

Inventor： Donhee Ham ,  Jeffrey T. Abbott ,  Hongkun Park ,  Wenxuan Wu

IPC: G01N33/487 ,  C12M1/42 ,  C12N13/00 ,  G01N27/00 ,  A61B5/053 ,  A61B5/24 ,  A61N1/18 ,  B82Y15/00

CPC classification number: G01N33/48728 ,  A61B5/053 ,  A61B5/24 ,  A61N1/18 ,  C12M35/02 ,  C12N13/00 ,  G01N27/00 ,  A61B2562/0285 ,  B82Y15/00

Abstract: Methods and systems for stimulating and monitoring electrogenic cells are described. Some systems for stimulating electrogenic cells are based on the injection of electric currents into the cells via electrodes connected to the cells. Such stimulators may comprise an impedance element having an input terminal and an output terminal coupled to an electrode, and a voltage follower coupled between the input terminal and the output terminal of the impedance element, the voltage follower being configured to maintain a substantially constant voltage between the input terminal and the output terminal of the impedance element. The impedance element may comprise one or more switched capacitors at least in some embodiments. In some embodiments, the voltage follower may be implemented using a source follower.

公开(公告)号：US11600774B2

公开(公告)日：2023-03-07

申请号：US17094121

申请日：2020-11-10

Applicant: Samsung Electronics Co., Ltd. ,  President and Fellows Of Harvard College

Inventor： Minhyun Lee ,  Houk Jang ,  Donhee Ham ,  Chengye Liu ,  Henry Julian Hinton ,  Haeryong Kim ,  Hyeonjin Shin

IPC: H01L45/00 ,  H01L27/24

Abstract: A nonvolatile memory device includes a resistance switching layer, a gate on the resistance switching layer, a gate oxide layer between the resistance switching layer and the gate, and a source and a drain, spaced apart from each other, on the resistance switching layer. A resistance value of the resistance switching layer is changed based on an illumination of light irradiated onto the resistance switching layer and is maintained as a changed resistance value.

公开(公告)号：US20230014082A1

公开(公告)日：2023-01-19

申请号：US17940174

申请日：2022-09-08

Applicant: President and Fellows of Harvard College

Inventor： Hongkun Park ,  Jeffrey T. Abbott ,  Wenxuan Wu ,  Tianyang Ye ,  Han Sae Jung ,  Donhee Ham

IPC: G01N27/327

Abstract: Disclosed herein are an apparatus for electrically assessing and/or manipulating cells. One aspect is directed to electrically mapping cells on the surface of the semiconductor substrate via cross-electrode impedance measurements. Further according to some aspects, the electrode array allows for spatially addressable electrical stimulation and/or recording of electrical signals in real-time using the CMOS circuitry. Some of these aspects are directed to using an electrode array to perform cell patterning through electrochemical gas generation, and extracellular electrochemical mapping.

公开(公告)号：US20220397512A1

公开(公告)日：2022-12-15

申请号：US17877702

申请日：2022-07-29

Applicant: President and Fellows of Harvard College

Inventor： Donhee Ham ,  Jeffrey T. Abbott ,  Wenxuan Wu ,  Tianyang Ye ,  Han Sae Jung ,  Hongkun Park

IPC: G01N15/10 ,  B01L3/00 ,  G01N33/487

Abstract: Disclosed herein are an apparatus for electrically assessing and/or manipulating cells. One aspect is directed to electrically mapping cells on the surface of the semiconductor substrate via cross-electrode impedance measurements. Further according to some aspects, the electrode array allows for spatially addressable electrical stimulation and/or recording of electrical signals in real-time using the CMOS circuitry. Some of these aspects are directed to using an electrode array to perform cell patterning through electrochemical gas generation, and extracellular electrochemical mapping.

公开(公告)号：US20210371846A1

公开(公告)日：2021-12-02

申请号：US16625603

申请日：2018-07-06

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donhee Ham ,  Jeffrey T ABBOTT ,  Hongkun PARK ,  Wenxuan WU

IPC: C12N13/00 ,  C12M1/42 ,  G01N27/00

Abstract: Methods and systems for stimulating and monitoring electrogenic cells are described. Some systems for stimulating electrogenic cells are based on the injection of electric currents into the cells via electrodes connected to the cells. Such stimulators may comprise an impedance element having an input terminal and an output terminal coupled to an electrode, and a voltage follower coupled between the input terminal and the output terminal of the impedance element, the voltage follower being configured to maintain a substantially constant voltage between the input terminal and the output terminal of the impedance element. The impedance element may comprise one or more switched capacitors at least in some embodiments. In some embodiments, the voltage follower may be implemented using a source follower.