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公开(公告)号：US5543576A
公开(公告)日：1996-08-06
申请号：US146422
申请日：1993-11-02
申请人： Albert J. J. van Ooijen , Krijn Rietveld , Andreas Hoekema , Jan Pen , Peter C. Sijmons , Teunis C. Verwoerd , Wilhemus J. Quax
发明人： Albert J. J. van Ooijen , Krijn Rietveld , Andreas Hoekema , Jan Pen , Peter C. Sijmons , Teunis C. Verwoerd , Wilhemus J. Quax
CPC分类号： C12Y301/03026 , A23K20/189 , C12N15/8242 , C12N15/8243 , C12N15/8257 , C12N9/16 , C12N9/2417
摘要： A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.
摘要翻译： 公开了使用含有增加量的酶的种子催化体外反应的方法。 该方法包括将优选以研磨形式的转基因非野生型种子加入到反应混合物中并允许种子中的酶增加反应速率。 通过将种子直接加入到反应混合物中，该方法提供了提取和纯化酶的昂贵且有问题的方法的解决方案。 还提供了治疗方法，其中缺乏足够供应酶的受试者以含有增加量的酶的种子的形式施用酶。
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公开(公告)号：US07033627B2
公开(公告)日：2006-04-25
申请号：US09149310
申请日：1998-02-02
申请人： Albert Johannes Joseph Van Ooyen , Krijn Rietveld , Wilhelmus Johannes Quax , Jan Pen , Andreas Hoekema , Peter Christiaan Sijmons , Teunis Cornelis Verwoerd
发明人： Albert Johannes Joseph Van Ooyen , Krijn Rietveld , Wilhelmus Johannes Quax , Jan Pen , Andreas Hoekema , Peter Christiaan Sijmons , Teunis Cornelis Verwoerd
CPC分类号： C12Y301/03026 , A21D8/042 , A23K20/189 , A23L29/06 , C12N9/16 , C12N9/2417 , C12N15/8242 , C12N15/8243 , C12N15/8257
摘要： A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture, especially a bakery process and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.
摘要翻译： 公开了使用含有增加量的酶的种子催化体外反应的方法。 该方法包括将转基因非野生型种子，优选以研磨形式加入到反应混合物，特别是面包店方法中，并允许种子中的酶提高反应速率。 通过将种子直接加入到反应混合物中，该方法提供了提取和纯化酶的昂贵且有问题的方法的解决方案。 还提供了治疗方法，其中缺乏足够供应酶的受试者以含有增加量的酶的种子的形式施用酶。
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公开(公告)号：US20060005286A1
公开(公告)日：2006-01-05
申请号：US11214413
申请日：2005-08-29
申请人： Albert Van Ooyen , Krijn Rietveld , Wilhelmus Quax , Jan Pen , Andreas Hoekema , Peter Sijmons , Teunis Verwoerd
发明人： Albert Van Ooyen , Krijn Rietveld , Wilhelmus Quax , Jan Pen , Andreas Hoekema , Peter Sijmons , Teunis Verwoerd
CPC分类号： C12Y301/03026 , A21D8/042 , A23K20/189 , A23L29/06 , C12N9/16 , C12N9/2417 , C12N15/8242 , C12N15/8243 , C12N15/8257
摘要： A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture, especially a bakery process and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the mehtod provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.
摘要翻译： 公开了使用含有增加量的酶的种子催化体外反应的方法。 该方法包括将转基因非野生型种子，优选以研磨形式加入到反应混合物，特别是面包店方法中，并允许种子中的酶提高反应速率。 通过将种子直接加入到反应混合物中，mehtod提供了解决提取和纯化酶的昂贵和有问题的过程的解决方案。 还提供了治疗方法，其中缺乏足够供应酶的受试者以含有增加量的酶的种子的形式施用酶。
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公开(公告)号：US5925804A
公开(公告)日：1999-07-20
申请号：US569150
申请日：1995-12-21
IPC分类号： A01N3/00 , A23L3/3562 , A23L3/42 , C12N1/21 , C12N5/10 , C12N9/10 , C12N9/12 , C12N15/11 , C12N15/54 , C12N15/82 , C12P19/12 , A01H5/00 , A01H5/10 , C12N5/14 , C12N15/31 , C12N15/52
CPC分类号： C12N9/1051 , A23L3/3562 , A23L3/42 , C12N15/8245 , C12N9/1066 , C12N9/12
摘要： A nucleic acid having (i) a DNA molecule which, when expressed in a plant or plant cell, increases the trehalose content of the plant or plant cell, the DNA molecule encoding an E. coli trehalose phosphate synthase, and (ii) a plant expressible promoter operatively coupled to the DNA molecule. Also, a method for obtaining a plant with increased trehalose production by introducing into a recipient cell of a plant, a plant expressible gene which, when expressed in a plant or plant cell increases the trehalose content of the plant or plant cell. The plant expressible gene is an E. coli trehalose phosphate synthase gene which is operably linked to: a) a transcriptional initiation region that is functional in the plant, and b) a DNA molecule encoding a selectable marker gene that is functional in the plant. The method includes a step of regenerating a plant from the recipient cell under conditions that allow for selection for the presence of the selectable marker gene.
摘要翻译： PCT No.PCT / EP94 / 02167 371 1995年12月21日第 102（e）日期1995年12月21日PCT提交1994年6月30日PCT公布。 公开号WO95 / 01446 日期1995年1月12日一种具有（i）DNA分子的核酸，其在植物或植物细胞中表达时，增加植物或植物细胞的海藻糖含量，编码大肠杆菌海藻糖磷酸合成酶的DNA分子，以及 （ii）可操作地偶联于DNA分子的植物可表达启动子。 此外，通过将植物或植物细胞的海藻糖含量提高到植物的受体细胞中可获得具有增加的海藻糖产生的植物的方法，当在植物或植物细胞中表达时植物可表达基因增加。 植物可表达基因是大肠杆菌海藻糖磷酸合酶基因，其可操作地连接到：a）在植物中有功能的转录起始区，和b）编码在植物中有功能的可选择标记基因的DNA分子。 该方法包括在允许选择存在选择性标记基因的条件下从受体细胞再生植物的步骤。
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公开(公告)号：US5763748A
公开(公告)日：1998-06-09
申请号：US829057
申请日：1997-03-31
申请人： Peter Christiaan Sijmons , Andreas Hoekema , Bernardus Martinus M. Dekker , Barbara Schrammeijer , Teunis Cornelius Verwoerd , Peturs Josephus M. Van Den Elzen
发明人： Peter Christiaan Sijmons , Andreas Hoekema , Bernardus Martinus M. Dekker , Barbara Schrammeijer , Teunis Cornelius Verwoerd , Peturs Josephus M. Van Den Elzen
IPC分类号： C12N15/09 , C07K14/415 , C07K14/765 , C12N5/10 , C12N15/82 , C12P21/02 , C12N5/00 , A01H5/00 , C12N15/14
CPC分类号： C12N15/8221 , C07K14/415 , C07K14/765 , C12N15/8216 , C12N15/8242 , C12N15/8257 , C07K2319/02
摘要： This invention provides for a method to produce excreted and correctly processed heterologous protein-material in a plant host. The method comprises the step of transforming a plant host using a recombinant polynucleotide, which comprises the DNA sequences encoding the fully processed heterologous protein material, directly preceded by a DNA sequence coding for a plant signal peptide, and regulatory sequences necessary for the plant host to express the heterologous gene construct, resulting in excretion of the heterologous protein from the cell and correct cleavage of the N-terminal signal peptide, so that the mature heterologous protein produced by the plant host is identical to the corresponding protein produced in its authentic host.
摘要翻译： 本发明提供了在植物宿主中产生排泄且正确处理的异源蛋白质材料的方法。 该方法包括使用重组多核苷酸转化植物宿主的步骤，其包含编码完全加工的异源蛋白质材料的DNA序列，其前面是编码植物信号肽的DNA序列，以及植物宿主所需的调节序列 表达异源基因构建体，导致来自细胞的异源蛋白质的排泄和N末端信号肽的正确切割，使得由植物宿主产生的成熟异源蛋白质与其真实宿主中产生的相应蛋白质相同。
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公开(公告)号：US5716802A
公开(公告)日：1998-02-10
申请号：US659287
申请日：1991-05-21
申请人： Peter Christiaan Sijmons , Andreas Hoekema , Bernardus Martinus M. Dekker , Barbara Schrammeijer , Teunis Cornelius Verwoerd , Peturs Josephus M. Van Den Elzen
发明人： Peter Christiaan Sijmons , Andreas Hoekema , Bernardus Martinus M. Dekker , Barbara Schrammeijer , Teunis Cornelius Verwoerd , Peturs Josephus M. Van Den Elzen
IPC分类号： C12N15/09 , C07K14/415 , C07K14/765 , C12N5/10 , C12N15/82 , C12P21/02 , A01H1/00 , A01H5/00 , C12N15/14
CPC分类号： C12N15/8221 , C07K14/415 , C07K14/765 , C12N15/8216 , C12N15/8242 , C12N15/8257 , C07K2319/02
摘要： This invention provides for a method to produce excreted and correctly processed heterologous protein-material in a plant host. The method comprises the step of transforming a plant host using a recombinant polynucleotide, which comprises the DNA sequences encoding the fully processed heterologous protein material, directly preceded by a DNA sequence coding for a plant signal peptide, and regulatory sequences necessary for the plant host to express the heterologous gene construct, resulting in excretion of the heterologous protein from the cell and correct cleavage of the N-terminal signal peptide, so that the mature heterologous protein produced by the plant host is identical to the corresponding protein produced in its authentic host.
摘要翻译： PCT No.PCT / NL90 / 00108 Sec。 371日期1991年3月21日 102（e）1991年3月21日PCT文件1990年7月26日PCT公布。 WO91 / 02066 PCT出版物 日期1991年2月21日本发明提供了在植物宿主中产生排泄物和正确加工的异源蛋白质材料的方法。 该方法包括使用重组多核苷酸转化植物宿主的步骤，其包含编码完全加工的异源蛋白质材料的DNA序列，其前面是编码植物信号肽的DNA序列，以及植物宿主所需的调节序列 表达异源基因构建体，导致来自细胞的异源蛋白质的排泄和N末端信号肽的正确切割，使得由植物宿主产生的成熟异源蛋白质与其真实宿主中产生的相应蛋白质相同。
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公开(公告)号：US5714474A
公开(公告)日：1998-02-03
申请号：US626554
申请日：1996-04-02
申请人： Albert J. J. Van Ooijen , Krijn Rietveld , Andreas Hoekema , Jan Pen , Peter Christian Sijmons , Teunis Cornelis Verwoerd , Wilhemus Johannes Quax
发明人： Albert J. J. Van Ooijen , Krijn Rietveld , Andreas Hoekema , Jan Pen , Peter Christian Sijmons , Teunis Cornelis Verwoerd , Wilhemus Johannes Quax
CPC分类号： C12Y301/03026 , A23K20/189 , C12N15/8242 , C12N15/8243 , C12N15/8257 , C12N9/16 , C12N9/2417
摘要： A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.
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