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公开(公告)号：US07109009B2
公开(公告)日：2006-09-19
申请号：US10405660
申请日：2003-04-02
申请人： C. Ron Wilson , David L. Craft , L. Dudley Eirich , Mark Eshoo , Krishna M. Madduri , Cathy A. Cornett , Alfred A. Brenner , Maria Tang , John C. Loper , Martin Gleeson
发明人： C. Ron Wilson , David L. Craft , L. Dudley Eirich , Mark Eshoo , Krishna M. Madduri , Cathy A. Cornett , Alfred A. Brenner , Maria Tang , John C. Loper , Martin Gleeson
CPC分类号： C12N9/0042 , C07K14/40 , C12N9/0071 , C12N9/0077 , C12P7/44
摘要： Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the ω-hydroxylase complex of C. tropicalis 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.
摘要翻译： 已经分离出编码热带假丝酵母20336的ω-羟化酶复合物的细胞色素P450和NADPH还原酶的新型基因。提供了包括这些基因，转染的宿主细胞和转化的宿主细胞的载体。 还提供了生产细胞色素P450和NADPH还原酶的方法，其涉及用编码这些酶的基因转化宿主细胞并培养细胞。 还提供了增加二羧酸生产的方法和增加上述酶的生产的方法，其涉及在宿主细胞中增加编码这些酶的基因的数量。 还提供了通过定量基因表达来区分基因家族成员的方法。
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公开(公告)号：US20120171726A1
公开(公告)日：2012-07-05
申请号：US13339633
申请日：2011-12-29
申请人： Mark Eshoo , Stanley Motley , John Picuri
发明人： Mark Eshoo , Stanley Motley , John Picuri
CPC分类号： C12Q1/686 , C12P19/34 , C12Q1/6846 , C12Q2521/525 , C12Q2525/179 , C12Q2527/125
摘要： The present invention provides compositions and methods for rapidly amplifying target nucleic acid (e.g., using whole genome amplification) that allows small amounts of starting nucleic acid to be employed. In certain embodiments, the methods employ compositions that comprise: phi29 polymerase, exo- Klenow polymerase and/or Klenow polymerase, dNTPs, primers, and a buffering agent. In some embodiments, the target nucleic acid is amplified at a rate that would result in at least 1000-fold amplification in thirty minutes.
摘要翻译： 本发明提供用于快速扩增允许使用少量起始核酸的靶核酸（例如，使用全基因组扩增）的组合物和方法。 在某些实施方案中，所述方法使用包含以下的组合物：phi29聚合酶，ex-Klenow聚合酶和/或Klenow聚合酶，dNTP，引物和缓冲剂。 在一些实施方案中，以在30分钟内至少产生1000倍扩增的速率扩增靶核酸。
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13.发明授权Cytochrome P450 monooxygenase and NADPH cytochrome P450 oxidoreductase genes and proteins related to the omega hydroxylase complex of Candida tropicalis and methods relating thereto 有权细胞色素P450单加氧酶和NADPH细胞色素P450氧化还原酶基因和与热带假丝酵母的ω羟化酶复合物有关的蛋白质及其相关方法公开(公告)号：US06331420B1
公开(公告)日：2001-12-18
申请号：US09302620
申请日：1999-04-30
申请人： C. Ron Wilson , David L. Craft , L. Dudley Eirich , Mark Eshoo , Krishna M. Madduri , Cathy A. Cornett , Alfred A. Brenner , Maria Tang , John C. Loper , Martin Gleeson
发明人： C. Ron Wilson , David L. Craft , L. Dudley Eirich , Mark Eshoo , Krishna M. Madduri , Cathy A. Cornett , Alfred A. Brenner , Maria Tang , John C. Loper , Martin Gleeson
IPC分类号： C12P746
CPC分类号： C12N9/0042 , C07K14/40 , C12N9/0071 , C12N9/0077 , C12P7/44
摘要： Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the &ohgr;-hydroxylase complex of C. tropicalis 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.
摘要翻译： 已经分离出编码热带假丝酵母20336的ω-羟化酶复合物的细胞色素P450和NADPH还原酶的新型基因。提供了包括这些基因，转染的宿主细胞和转化的宿主细胞的载体。 还提供了生产细胞色素P450和NADPH还原酶的方法，其涉及用编码这些酶的基因转化宿主细胞并培养细胞。 还提供了增加二羧酸生产的方法和增加上述酶的生产的方法，其涉及在宿主细胞中增加编码这些酶的基因的数量。 还提供了通过定量基因表达来区分基因家族成员的方法。
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公开(公告)号：US09428801B2
公开(公告)日：2016-08-30
申请号：US13339633
申请日：2011-12-29
申请人： Mark Eshoo , Stanley Motley , John Picuri
发明人： Mark Eshoo , Stanley Motley , John Picuri
CPC分类号： C12Q1/686 , C12P19/34 , C12Q1/6846 , C12Q2521/525 , C12Q2525/179 , C12Q2527/125
摘要： The present invention provides compositions and methods for rapidly amplifying target nucleic acid (e.g., using whole genome amplification) that allows small amounts of starting nucleic acid to be employed. In certain embodiments, the methods employ compositions that comprise: phi29 polymerase, exo- Klenow polymerase and/or Klenow polymerase, dNTPs, primers, and a buffering agent. In some embodiments, the target nucleic acid is amplified at a rate that would result in at least 1000-fold amplification in thirty minutes.
摘要翻译： 本发明提供用于快速扩增允许使用少量起始核酸的靶核酸（例如，使用全基因组扩增）的组合物和方法。 在某些实施方案中，所述方法使用包含以下的组合物：phi29聚合酶，ex-Klenow聚合酶和/或Klenow聚合酶，dNTP，引物和缓冲剂。 在一些实施方案中，以在30分钟内至少产生1000倍扩增的速率扩增靶核酸。
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公开(公告)号：US20130274147A1
公开(公告)日：2013-10-17
申请号：US13925355
申请日：2013-06-24
申请人： Mark Eshoo , John Picuri
发明人： Mark Eshoo , John Picuri
IPC分类号： C12Q1/68
CPC分类号： B01L3/5027 , B01L3/502753 , B01L7/52 , B01L9/527 , B01L2200/10 , B01L2200/16 , B01L2300/0672 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/0887 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0683 , C12N9/96 , C12Q1/6806 , C12Q1/6874
摘要： The present invention provides integrated sample preparation systems and stabilized enzyme mixtures. In particular, the present invention provides microfluidic cards configured for processing a sample and generating DNA libraries that are suitable for use in sequencing methods (e.g., next generation sequencing methods) or other suitable nucleic acid analysis methods. The present invention also provides stabilized enzyme mixtures containing an enzyme (e.g., an enzyme used in whole genome amplification), BSA, and a sugar. Such enzyme mixtures may be lyophilized and stored at room temperature without significant loss of enzyme activity for months.
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公开(公告)号：US20060014154A1
公开(公告)日：2006-01-19
申请号：US10891337
申请日：2004-07-14
申请人： Mark Eshoo
发明人： Mark Eshoo
CPC分类号： C12Q1/6806 , C12Q2521/531 , C12Q2521/501 , C12Q2521/301 , C12Q2521/514
摘要： The present invention provides methods and kits for repair of degraded DNA which may then be used as a template for efficient amplification by a number of different amplification reactions. The method relies upon a series of enzymatic activities provided by DNA repair enzymes.
摘要翻译： 本发明提供了用于修复降解的DNA的方法和试剂盒，然后可以将其用作通过许多不同扩增反应进行有效扩增的模板。 该方法依赖于DNA修复酶提供的一系列酶活性。
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公开(公告)号：US09109222B2
公开(公告)日：2015-08-18
申请号：US13337828
申请日：2011-12-27
申请人： Mark Eshoo , John Picuri , Stanley Motley
发明人： Mark Eshoo , John Picuri , Stanley Motley
CPC分类号： B01J19/0046 , B01J2219/00585 , B01J2219/00722 , C12N15/10 , C12N15/1093 , C12Q1/686 , C12Q2521/501 , C12Q2525/191
摘要： The present invention provides compositions and methods for preparing a nucleic acid library in a multi-purpose buffer (e.g., employing whole genome amplification), where nucleic acid purification is not required between or during steps. In certain embodiments, small amounts of starting nucleic acid (e.g., genomic DNA) are employed and the steps are accomplished in a single container. In some embodiments, the nucleic acid library is subjected to sequencing methodologies or rolling circle amplification.
摘要翻译： 本发明提供了用于在多用途缓冲液（例如，使用全基因组扩增）中制备核酸文库的组合物和方法，其中在步骤之间或期间不需要核酸纯化。 在某些实施方案中，使用少量的起始核酸（例如，基因组DNA），并且步骤在单个容器中完成。 在一些实施方案中，对核酸文库进行测序方法学或滚环扩增。
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公开(公告)号：US20120172258A1
公开(公告)日：2012-07-05
申请号：US13337828
申请日：2011-12-27
申请人： Mark Eshoo , John Picuri , Stanley Motley
发明人： Mark Eshoo , John Picuri , Stanley Motley
CPC分类号： B01J19/0046 , B01J2219/00585 , B01J2219/00722 , C12N15/10 , C12N15/1093 , C12Q1/686 , C12Q2521/501 , C12Q2525/191
摘要： The present invention provides compositions and methods for preparing a nucleic acid library in a multi-purpose buffer (e.g., employing whole genome amplification), where nucleic acid purification is not required between or during steps. In certain embodiments, small amounts of starting nucleic acid (e.g., genomic DNA) are employed and the steps are accomplished in a single container. In some embodiments, the nucleic acid library is subjected to sequencing methodologies or rolling circle amplification.
摘要翻译： 本发明提供了在多用途缓冲液（例如，使用全基因组扩增）中制备核酸文库的组合物和方法，其中在步骤之间或期间不需要核酸纯化。 在某些实施方案中，使用少量的起始核酸（例如，基因组DNA），并且步骤在单个容器中完成。 在一些实施方案中，对核酸文库进行测序方法学或滚环扩增。
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19.发明授权公开(公告)号：US07063972B2
公开(公告)日：2006-06-20
申请号：US10138838
申请日：2002-05-03
申请人： C. Ron Wilson , David L. Craft , L. Dudley Eirich , Mark Eshoo , Krishna M. Madduri , Cathy A. Cornett , Alfred A. Brenner , Maria Tang , John C. Loper , Martin Gleeson
发明人： C. Ron Wilson , David L. Craft , L. Dudley Eirich , Mark Eshoo , Krishna M. Madduri , Cathy A. Cornett , Alfred A. Brenner , Maria Tang , John C. Loper , Martin Gleeson
CPC分类号： C12N9/0042 , C07K14/40 , C12N9/0071 , C12N9/0077 , C12P7/44
摘要： Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the ω-hydroxylase complex of C. tropicalis 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.
摘要翻译： 已经分离出编码热带假丝酵母20336的ω-羟化酶复合物的细胞色素P450和NADPH还原酶的新型基因。提供了包括这些基因，转染的宿主细胞和转化的宿主细胞的载体。 还提供了生产细胞色素P450和NADPH还原酶的方法，其涉及用编码这些酶的基因转化宿主细胞并培养细胞。 还提供了增加二羧酸生产的方法和增加上述酶的生产的方法，其涉及在宿主细胞中增加编码这些酶的基因的数量。 还提供了通过定量基因表达来区分基因家族成员的方法。
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20.发明授权公开(公告)号：US07049112B2
公开(公告)日：2006-05-23
申请号：US10139296
申请日：2002-05-03
申请人： C. Ron Wilson , David L. Craft , L. Dudley Eirich , Mark Eshoo , Krishna M. Madduri , Cathy A. Cornett , Alfred A. Brenner , Maria Tang , John C. Loper , Martin Gleeson
发明人： C. Ron Wilson , David L. Craft , L. Dudley Eirich , Mark Eshoo , Krishna M. Madduri , Cathy A. Cornett , Alfred A. Brenner , Maria Tang , John C. Loper , Martin Gleeson
CPC分类号： C12N9/0042 , C07K14/40 , C12N9/0071 , C12N9/0077 , C12P7/44
摘要： Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the ω-hydroxylase complex of C. tropicalis 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.
摘要翻译： 已经分离出编码热带假丝酵母20336的ω-羟化酶复合物的细胞色素P450和NADPH还原酶的新型基因。提供了包括这些基因，转染的宿主细胞和转化的宿主细胞的载体。 还提供了生产细胞色素P450和NADPH还原酶的方法，其涉及用编码这些酶的基因转化宿主细胞并培养细胞。 还提供了增加二羧酸生产的方法和增加上述酶的生产的方法，其涉及在宿主细胞中增加编码这些酶的基因的数量。 还提供了通过定量基因表达来区分基因家族成员的方法。
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