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    RAPID WHOLE GENOME AMPLIFICATION
    1.
    发明申请
    RAPID WHOLE GENOME AMPLIFICATION 有权
    快速全基因扩增

    公开(公告)号：US20120171726A1

    公开(公告)日：2012-07-05

    申请号：US13339633

    申请日：2011-12-29

    申请人： Mark Eshoo ,  Stanley Motley ,  John Picuri

    发明人： Mark Eshoo ,  Stanley Motley ,  John Picuri

    IPC分类号： C12P19/34 ,  C12N9/12

    CPC分类号： C12Q1/686 ,  C12P19/34 ,  C12Q1/6846 ,  C12Q2521/525 ,  C12Q2525/179 ,  C12Q2527/125

    摘要： The present invention provides compositions and methods for rapidly amplifying target nucleic acid (e.g., using whole genome amplification) that allows small amounts of starting nucleic acid to be employed. In certain embodiments, the methods employ compositions that comprise: phi29 polymerase, exo- Klenow polymerase and/or Klenow polymerase, dNTPs, primers, and a buffering agent. In some embodiments, the target nucleic acid is amplified at a rate that would result in at least 1000-fold amplification in thirty minutes.

    摘要翻译： 本发明提供用于快速扩增允许使用少量起始核酸的靶核酸（例如，使用全基因组扩增）的组合物和方法。 在某些实施方案中，所述方法使用包含以下的组合物：phi29聚合酶，ex-Klenow聚合酶和/或Klenow聚合酶，dNTP，引物和缓冲剂。 在一些实施方案中，以在30分钟内至少产生1000倍扩增的速率扩增靶核酸。

    Rapid whole genome amplification
    3.
    发明授权
    Rapid whole genome amplification 有权
    快速全基因组扩增

    公开(公告)号：US09428801B2

    公开(公告)日：2016-08-30

    申请号：US13339633

    申请日：2011-12-29

    申请人： Mark Eshoo ,  Stanley Motley ,  John Picuri

    发明人： Mark Eshoo ,  Stanley Motley ,  John Picuri

    IPC分类号： C12P19/34 ,  C12Q1/68

    CPC分类号： C12Q1/686 ,  C12P19/34 ,  C12Q1/6846 ,  C12Q2521/525 ,  C12Q2525/179 ,  C12Q2527/125

    摘要： The present invention provides compositions and methods for rapidly amplifying target nucleic acid (e.g., using whole genome amplification) that allows small amounts of starting nucleic acid to be employed. In certain embodiments, the methods employ compositions that comprise: phi29 polymerase, exo- Klenow polymerase and/or Klenow polymerase, dNTPs, primers, and a buffering agent. In some embodiments, the target nucleic acid is amplified at a rate that would result in at least 1000-fold amplification in thirty minutes.

    摘要翻译： 本发明提供用于快速扩增允许使用少量起始核酸的靶核酸（例如，使用全基因组扩增）的组合物和方法。 在某些实施方案中，所述方法使用包含以下的组合物：phi29聚合酶，ex-Klenow聚合酶和/或Klenow聚合酶，dNTP，引物和缓冲剂。 在一些实施方案中，以在30分钟内至少产生1000倍扩增的速率扩增靶核酸。

    Nucleic acid sample preparation methods and compositions
    5.
    发明授权
    Nucleic acid sample preparation methods and compositions 有权
    核酸样品制备方法和组合物

    公开(公告)号：US09109222B2

    公开(公告)日：2015-08-18

    申请号：US13337828

    申请日：2011-12-27

    申请人： Mark Eshoo ,  John Picuri ,  Stanley Motley

    发明人： Mark Eshoo ,  John Picuri ,  Stanley Motley

    IPC分类号： C40B50/06 ,  C12N9/12 ,  C09K3/00 ,  C12N15/10 ,  C12Q1/68

    CPC分类号： B01J19/0046 ,  B01J2219/00585 ,  B01J2219/00722 ,  C12N15/10 ,  C12N15/1093 ,  C12Q1/686 ,  C12Q2521/501 ,  C12Q2525/191

    摘要： The present invention provides compositions and methods for preparing a nucleic acid library in a multi-purpose buffer (e.g., employing whole genome amplification), where nucleic acid purification is not required between or during steps. In certain embodiments, small amounts of starting nucleic acid (e.g., genomic DNA) are employed and the steps are accomplished in a single container. In some embodiments, the nucleic acid library is subjected to sequencing methodologies or rolling circle amplification.

    摘要翻译： 本发明提供了用于在多用途缓冲液（例如，使用全基因组扩增）中制备核酸文库的组合物和方法，其中在步骤之间或期间不需要核酸纯化。 在某些实施方案中，使用少量的起始核酸（例如，基因组DNA），并且步骤在单个容器中完成。 在一些实施方案中，对核酸文库进行测序方法学或滚环扩增。

    NUCLEIC ACID SAMPLE PREPARATION METHODS AND COMPOSITIONS
    6.
    发明申请
    NUCLEIC ACID SAMPLE PREPARATION METHODS AND COMPOSITIONS 有权
    核酸样品制备方法和组合物

    公开(公告)号：US20120172258A1

    公开(公告)日：2012-07-05

    申请号：US13337828

    申请日：2011-12-27

    申请人： Mark Eshoo ,  John Picuri ,  Stanley Motley

    发明人： Mark Eshoo ,  John Picuri ,  Stanley Motley

    IPC分类号： C40B50/06 ,  C12N9/12 ,  C09K3/00

    CPC分类号： B01J19/0046 ,  B01J2219/00585 ,  B01J2219/00722 ,  C12N15/10 ,  C12N15/1093 ,  C12Q1/686 ,  C12Q2521/501 ,  C12Q2525/191

    摘要： The present invention provides compositions and methods for preparing a nucleic acid library in a multi-purpose buffer (e.g., employing whole genome amplification), where nucleic acid purification is not required between or during steps. In certain embodiments, small amounts of starting nucleic acid (e.g., genomic DNA) are employed and the steps are accomplished in a single container. In some embodiments, the nucleic acid library is subjected to sequencing methodologies or rolling circle amplification.

    摘要翻译： 本发明提供了在多用途缓冲液（例如，使用全基因组扩增）中制备核酸文库的组合物和方法，其中在步骤之间或期间不需要核酸纯化。 在某些实施方案中，使用少量的起始核酸（例如，基因组DNA），并且步骤在单个容器中完成。 在一些实施方案中，对核酸文库进行测序方法学或滚环扩增。

    Multiple displacement amplification
    7.
    发明授权
    Multiple displacement amplification 有权

    公开(公告)号：US09890408B2

    公开(公告)日：2018-02-13

    申请号：US12905819

    申请日：2010-10-15

    申请人： Mark W. Eshoo ,  John Picuri ,  Curtis Phillipson

    发明人： Mark W. Eshoo ,  John Picuri ,  Curtis Phillipson

    IPC分类号： C12Q1/64 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12P19/34 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2527/125 ,  C12Q2531/119 ,  C12Q2537/143 ,  C12Q2563/159 ,  C12Q2527/127 ,  C12Q2531/113

    摘要： The present invention provides methods kits and systems for performing multiple displacement amplification reactions. In one method a sample of nucleic acid is provided. The nucleic acid is contacted with a reaction mixture which includes a set of oligonucleotide primers, a one or more polymerase enzymes and a detergent. The reaction mixture is then subjected to conditions under which the nucleic acid sequence is amplified to produce an amplified product in a multiple displacement reaction. The method may also be carried out by contacting the nucleic acid with the reaction mixture in the form of an emulsion. A kit is also provided for carrying out either the methods described above. The kit includes one or more polymerases, a plurality of primers and a detergent. The kit may also include a hydrophobic polymer and may include instructions for performing a multiple displacement amplification reaction on a nucleic acid sample.