公开(公告)号：US08329106B2

公开(公告)日：2012-12-11

申请号：US11661410

申请日：2005-09-01

申请人： Han Oh Park ,  Hanee Park ,  Jong Soo Baek

发明人： Han Oh Park ,  Hanee Park ,  Jong Soo Baek

IPC分类号： G01N21/64 ,  B01J19/00

CPC分类号： G01N21/645 ,  B01L3/5025 ,  B01L7/525 ,  B01L2300/0838 ,  B01L2300/0841 ,  B01L2300/087

摘要： The present invention relates to a apparatus for quantitative continuous real-time monitoring to monitor a continuous reaction of biochemical reagent and the reaction, such as DNA. More particularly, the present invention is directed to a miniaturized apparatus for real-time monitoring of biochemical reaction, which comprises capillary tubes (100) wherein biochemical reaction mixture flow; a thermal conduction block (120) which is coiled with capillary tube several rounds in order and composed of several blocks for temperature control of which temperatures are different from each other for heating or cooling the biochemical reaction mixture which flow in capillary tube, and a temperature controller which controls the temperature of above temperature control block; a radiation part (130) to radiate the reaction mixture flowing through the capillary tube and a light receiving section (140) which receives and measures the intensity of the fluorescence generated from the capillary tubes.

摘要翻译： 本发明涉及用于定量连续实时监测的装置，用于监测生物化学试剂和反应如DNA的连续反应。 更具体地，本发明涉及一种用于实时监测生物化学反应的小型化装置，其包括其中生物化学反应混合物流动的毛细管（100） 热传导块（120），其顺序地由毛细管螺旋成几圈，并且由用于温度彼此不同的温度控制的几个块组成，用于加热或冷却在毛细管中流动的生物化学反应混合物，温度 控制器控制上述温度控制块的温度; 用于辐射流过毛细管的反应混合物的辐射部分（130）和接收并测量从毛细管产生的荧光强度的光接收部分（140）。

公开(公告)号：US08058007B2

公开(公告)日：2011-11-15

申请号：US12531778

申请日：2008-03-19

申请人： Hui Jeong Jeong ,  Min-Jung Kim ,  Hae-Joon Park ,  Han Oh Park

发明人： Hui Jeong Jeong ,  Min-Jung Kim ,  Hae-Joon Park ,  Han Oh Park

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6846 ,  C12Q2537/155 ,  C12Q2527/101 ,  C12Q2521/107

摘要： Disclosed is a cyclic reverse transcription method, which comprises performing reverse transcription reaction in one or more cycles, each cycle comprising the steps of: (i) performing reaction with a reverse transcription reaction solution comprising template RNA, RNA-dependent DNA polymerase, a reaction buffer, primers for reverse transcription and dNTPs, and optionally, a stabilizer, at 10° C. to 40° C. and, (ii) performing reaction with the resultant reaction solution at 42° to 55° C.

摘要翻译： 公开了一种循环逆转录方法，其包括在一个或多个循环中进行逆转录反应，每个循环包括以下步骤：（i）与包含模板RNA，RNA依赖性DNA聚合酶的反转录反应溶液，反应 缓冲液，用于逆转录的引物和dNTP，以及任选的稳定剂，在10℃至40℃下，和（ii）在42℃至55℃下与所得反应溶液进行反应。

公开(公告)号：US20100221786A1

公开(公告)日：2010-09-02

申请号：US12531778

申请日：2008-03-19

申请人： Hui Jeong Jeong ,  Min-Jung Kim ,  Hae-Joon Park ,  Han Oh Park

发明人： Hui Jeong Jeong ,  Min-Jung Kim ,  Hae-Joon Park ,  Han Oh Park

IPC分类号： C12P19/34

CPC分类号： C12Q1/6846 ,  C12Q2537/155 ,  C12Q2527/101 ,  C12Q2521/107

摘要： Disclosed is a cyclic reverse transcription method, which comprises performing reverse transcription reaction in one or more cycles, each cycle comprising the steps of: (i) performing reaction with a reverse transcription reaction solution comprising template RNA, RNA-dependent DNA polymerase, a reaction buffer, primers for reverse transcription and dNTPs, and optionally, a stabilizer, at 10° C. to 40° G and, (ii) performing reaction with the resultant reaction solution at 42° to 55° C.

摘要翻译： 公开了一种循环逆转录方法，其包括在一个或多个循环中进行逆转录反应，每个循环包括以下步骤：（i）与包含模板RNA，RNA依赖性DNA聚合酶的反转录反应溶液，反应 缓冲液，用于逆转录的引物和dNTP，以及任选的稳定剂，在10℃至40℃下，和（ii）在42℃至55℃下与所得反应溶液进行反应。

公开(公告)号：US20100209973A1

公开(公告)日：2010-08-19

申请号：US12682456

申请日：2008-10-28

申请人： Seong-Youl Kim ,  Hyun Bae Kim ,  Hae-Joon Park ,  Han Oh Park

发明人： Seong-Youl Kim ,  Hyun Bae Kim ,  Hae-Joon Park ,  Han Oh Park

IPC分类号： C12P19/34 ,  C12N9/14

CPC分类号： C12Q1/686 ,  C12Q2549/101 ,  C12Q2527/125

摘要： The present invention relates to a dried composition for hot-start PCR, more precisely a dried composition for hot-start PCR with improved stability and long-term storagability which is characteristically prepared by the steps of preparing a reaction mixture by mixing an aqueous solution containing reaction buffer, MgCl2, 4 types of dNTPs, DNA polymerase with pyrophosphate and pyrophosphatase in a reaction tube; and drying the reaction mixture prepared above, a preparation method of the same and a method for amplifying nucleic acid using the same. The dried composition for hot-start PCR is added with pyrophosphate and pyrophosphatase together before drying, so that it can have improved stability and long-term storagability as well as convenience in use, compared with the conventional compositions for hot-start PCR. Therefore, this composition can be effectively used for hot-start PCR, multiplex PCR or real-time quantitative PCR.

摘要翻译： 本发明涉及一种用于热启动PCR的干燥组合物，更准确地说是一种用于热启动PCR的干燥组合物，其具有改进的稳定性和长期储存性，其特征在于通过以下步骤制备反应混合物：将含有 反应缓冲液，MgCl 2，4种dNTP，反应管中含有焦磷酸的DNA聚合酶和焦磷酸酶; 并干燥上述制备的反应混合物，其制备方法和使用其制备用于扩增核酸的方法。 用于热启动PCR的干燥组合物在干燥之前加入焦磷酸盐和焦磷酸酶，与常规的热启动组合物相比，其可以具有改善的稳定性和长期储存性以及使用方便性。 因此，该组合物可有效用于热启动PCR，多重PCR或实时定量PCR。

公开(公告)号：US20100203025A1

公开(公告)日：2010-08-12

申请号：US12376368

申请日：2007-05-14

申请人： Ji Hee Kang ,  Byeung Il You ,  Sung Il Yun ,  Han Oh Park

发明人： Ji Hee Kang ,  Byeung Il You ,  Sung Il Yun ,  Han Oh Park

IPC分类号： A61K35/74 ,  C12N1/20 ,  A23L1/28 ,  A23K3/00 ,  C07K14/335 ,  C07H21/04 ,  C12N15/63 ,  A61P3/04

CPC分类号： C12R1/225 ,  A23C9/1234 ,  A23L33/135 ,  A23L33/18 ,  A23Y2220/37 ,  A61K38/00 ,  C07K14/335 ,  C12N1/20

摘要： The present invention relates to a lactic acid bacterium isolated from human mother's milk, more precisely a Lactobacillus gasseri BNR17 strain that is isolated from Korean mother's milk and has excellent probiotic activity including acid resistance, bile acid resistance and antimicrobial activity and weight gaining inhibitory effect as well Again, the Lactobacillus gasseri BNR17 of the present invention has excellent acid resistance, bile acid resistance, enteric absorption activity and antimicrobial activity against pathogenic microorganisms, in addition to the weight gaining inhibitory effect by synthesizing indigestible polysaccharides from monosaccharides included in food taken and releasing the synthesized polysaccharides out of the body. Therefore, the strain of the invention, owing to such beneficiary effects, can be effectively used not only for the production of fermented milk, other fermented food products and animal feeds but also for the production of live cell products and food additives for preventing weight gaining.

摘要翻译： 本发明涉及从人母乳分离的乳酸菌，更准确地说是从韩国母乳中分离的加氏乳杆菌BNR17菌株，具有优异的益生菌活性，包括耐酸性，耐胆汁酸性和抗微生物活性和增重抑制作用 另外，本发明的加氏乳杆菌BNR17除了通过合成不可消化的多糖外，还包括在食物摄取和释放中包含的单糖，而且具有优异的抗酸性，耐胆汁酸性，肠吸收活性和抗病原微生物的抗微生物活性 合成的多糖出体外。 因此，由于这种受益效应，本发明的菌株不仅可以有效地用于生产发酵乳，其他发酵食品和动物饲料，而且可用于生产活细胞产品和食品添加剂，以防止体重增加 。

公开(公告)号：US20090155904A1

公开(公告)日：2009-06-18

申请号：US11721303

申请日：2005-12-08

申请人： Young-Chul Choi ,  Han Oh Park ,  Sorim Choung ,  Young Joo Kim ,  Sang Soo Kim ,  Seong-min Park ,  Sang-Cheol Kim ,  Gyuman Yoon ,  Kyoung Oak Choi ,  Hyo Jin Kang

发明人： Young-Chul Choi ,  Han Oh Park ,  Sorim Choung ,  Young Joo Kim ,  Sang Soo Kim ,  Seong-min Park ,  Sang-Cheol Kim ,  Gyuman Yoon ,  Kyoung Oak Choi ,  Hyo Jin Kang

IPC分类号： C12N5/02

CPC分类号： C12N15/111 ,  C12N15/113 ,  C12N2310/14 ,  C12N2320/11 ,  G16B20/00

摘要： A inhibition method of target mRNA expression includes: (a) obtaining binding energy of a double combination section on a dsRNA sequence of all combination comprising complementary nucleotides to a random target mRNA; (b) dividing the binding energy into four sections on the dsRNA sequence of each combination to obtain a difference of the mean binding energy between each section and convert into a score of a relative combination energy pattern; (c) selecting siRNA whose inhibition efficiency to target mRNA is expected to be high by applying the converted score to the dsRNA sequence with other factors that affect the efficiency of siRNA; and (d) inhibiting target mRNA expression using the selected siRNA. As a result, a researcher or an experimenter can analyze patterns of a relative binding energy on base sequences of unknown siRNA without actual experiments to determine whether the siRNA is effective or ineffective rapidly, thereby design and production efficiency of siRNA can be maximized and target mRNA can be effectively inhibited with efficient siRNA to the target mRNA.

摘要翻译： 靶mRNA表达的抑制方法包括：（a）获得双组合切片对包含与随机靶mRNA的互补核苷酸的所有组合的dsRNA序列的结合能; （b）将结合能分成每个组合的dsRNA序列上的四个部分，以获得每个部分之间的平均结合能的差异，并转换成相对组合能量模式的得分; （c）通过将影响siRNA效率的其它因素应用于dsRNA序列的转化得分，选择siRNA靶向mRNA的抑制效率高的siRNA; 和（d）使用所选择的siRNA抑制靶mRNA表达。 因此，研究人员或实验者可以分析未知siRNA基因序列相对结合能力的模式，无需实际实验，可以快速确定siRNA是否有效或无效，从而可以使siRNA的设计和生产效率最大化，靶mRNA 可以有效地抑制靶mRNA的有效siRNA。

公开(公告)号：US20080220415A1

公开(公告)日：2008-09-11

申请号：US10593900

申请日：2005-03-25

申请人： Han Oh Park ,  Hyun-Bae Kim ,  Sung-Min Chi

发明人： Han Oh Park ,  Hyun-Bae Kim ,  Sung-Min Chi

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6844 ,  C12Q1/686 ,  C12Q2563/173 ,  C12Q2563/107 ,  C12Q2561/113

摘要： The present invention relates to a detection method of nucleic acid amplification using probe labeled with intercalating dye. More particularly, the present invention is directed to a real-time detection method of nucleic acid amplification, comprising the steps of i) producing an aqueous buffer which contains a nucleic acid, a pair of primers for amplification of said nucleic acid, a fluorescent probe wherein a fluorescent dye of which intensity of fluorescence is varied when the dye is intercalated into a double-stranded nucleic acid, is connected with an oligonucleotide of which base sequence is complementary with at least a part of said nucleic acid, four (4) kinds of nucleotides and DNA polymerase; ii) denaturing said doublestranded nucleic acid into single strands by heating the aqueous buffer prepared in step i) up to 931 C to 96 C; iii) annealing said pair of primers with said single strand by cooling the solution obtained in step ii) up to 50 C. to 571 C; iv) replicating said single-stranded nucleic acid by heating the solution obtained from step iii) up to 701 C to 74° C.; v) denaturing said replicated nucleic add into single strands by heating the solution obtained in step iv) up to 931 C to 961 C; vi) annealing said fluorescent probe with said single-stranded nucleic acid by cooling the solution obtained in step v up to 501 C to 57 C; vii) measuring an intensity of the fluorescence emitted from the solution obtained in step vi); and viii) repeating more than one steps iv) through vii).

摘要翻译： 本发明涉及使用用插层染料标记的探针的核酸扩增检测方法。 更具体地，本发明涉及核酸扩增的实时检测方法，包括以下步骤：i）产生含有核酸的水性缓冲液，用于扩增所述核酸的一对引物，荧光探针 其中当染料插入双链核酸时荧光强度变化的荧光染料与其碱基序列与所述核酸的至少一部分互补的寡核苷酸连接，四（4）种 的核苷酸和DNA聚合酶; ii）通过加热步骤i）中制备的含水缓冲液至931℃至96℃，将所述双链核酸变性为单链; iii）通过将步骤ii）中获得的溶液冷却至50℃至571℃，用所述单链退火所述一对引物; iv）通过将从步骤iii）获得的溶液加热至701℃至74℃来复制所述单链核酸; v）通过将步骤iv）中获得的溶液加热至931℃至961℃，将所述复制的核酸添加到单链中; vi）通过将步骤v中获得的溶液冷却至501℃至57℃，用所述单链核酸退火所述荧光探针; vii）测量从步骤vi）中获得的溶液发射的荧光的强度; 和viii）重复多于一个步骤iv）至vii）。

公开(公告)号：US06576190B1

公开(公告)日：2003-06-10

申请号：US09869556

申请日：2001-08-21

申请人： Han Oh Park

发明人： Han Oh Park

IPC分类号： A61L200

CPC分类号： A61L2/202 ,  A61G7/05784 ,  A61G13/108 ,  A61L2/10

摘要： A method and apparatus for sterilizing and disinfecting bedclothes. The method includes preparing a sterilizing room defined by a cover; placing bedclothes into the sterilizing room; and exposing the bedclothes to ultraviolet rays and ozone generated by a generator in communication with the sterilizing room. The apparatus includes a generator for generating ultraviolet rays and ozone and a cover for defining a sterilizing room to accommodate the bedclothes.

摘要翻译： 一种消毒和消毒床上用品的方法和装置。 该方法包括准备由盖限定的消毒室; 将床上用品放入消毒室; 并将床单暴露于与灭菌室连通的发生器产生的紫外线和臭氧。 该装置包括用于产生紫外线和臭氧的发生器和用于限定消毒室以容纳床上用品的盖子。

公开(公告)号：US09850530B2

公开(公告)日：2017-12-26

申请号：US13881900

申请日：2011-10-27

申请人： Han Oh Park ,  Kwon Sic Kim ,  Yang Won Lee ,  Jin Il Lee ,  Byung Rae Jeong ,  Jong Hoon Kim

发明人： Han Oh Park ,  Kwon Sic Kim ,  Yang Won Lee ,  Jin Il Lee ,  Byung Rae Jeong ,  Jong Hoon Kim

IPC分类号： C12Q1/68 ,  B01L3/02 ,  C12Q1/02 ,  G01N35/02 ,  B01L7/00 ,  G01N35/00 ,  G01N35/04

CPC分类号： C12Q1/686 ,  B01L3/0227 ,  B01L3/0234 ,  B01L7/52 ,  B01L2300/0829 ,  B01L2300/1805 ,  B01L2400/0478 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/26 ,  C12Q1/02 ,  C12Q1/6844 ,  G01N35/0098 ,  G01N35/028 ,  G01N2035/0425 ,  G01N2035/0465

摘要： The present invention relates to an automatic real-time quantitative amplification system which can perform analysis of various biological samples, and more particularly to an automatic real-time quantitative amplification system in which a plurality of decks for respectively accommodating biological samples are put in a deck storing/transferring device, whereby it is possible to automatically analyze an amount or existence of a target substance containing a target nucleic acid in the biologic sample, such as a particular gene, a particular, a particular pathogenic bacterium and a particular protein, by amplifying the target nucleic acid purified by some processes of purification, purification after culture, or purification after reaction of the target substance contained in the biological sample and then checking an amount of the amplified target nucleic acid.

公开(公告)号：US09534210B2

公开(公告)日：2017-01-03

申请号：US13984746

申请日：2012-02-07

申请人： Han Oh Park ,  Sung Jun Yang ,  Sung Mo Joo ,  Byoung Oh Hwang

发明人： Han Oh Park ,  Sung Jun Yang ,  Sung Mo Joo ,  Byoung Oh Hwang

IPC分类号： C12N9/12 ,  C12N1/20 ,  C12N15/70 ,  C12P19/34

CPC分类号： C12N9/1276 ,  C12N1/20 ,  C12N9/12 ,  C12N15/70 ,  C12P19/34 ,  Y02P20/52

摘要： The present invention relates to a reverse transcriptase having improved thermostability, more precisely a mutant reverse transcriptase with improved thermostability by substitution of one or more amino acids selected from the group consisting of the 63rd glutamine (Q63), the 264th lysine (K264), the 295th lysine (K295), the 306th threonine (T306), the 346th glutamic acid (E346), the 408th proline (P408), the 438th histidine (H438), and the 454th asparagin (N454) of the amino acid sequence of M-MLV originated reverse transcriptase represented by SEQ. ID. NO: 1 with other amino acids. The mutant reverse transcriptase of the present invention demonstrates excellent thermostability, compared with the wild type reverse transcriptase. Therefore, it is advantageous to obtain the target cDNA with stable reverse transcription activity even in the presence of RNA that can form the stable secondary structure at a high temperature.

摘要翻译： 本发明涉及具有改善的热稳定性的逆转录酶，更准确地说，具有通过取代选自第63位谷氨酰胺（Q63），第264位赖氨酸（K264）， 第309位赖氨酸（K295），第306位苏氨酸（T306），第346位谷氨酸（E346），第408位脯氨酸（P408），第438位组氨酸（H438）和第454位天门冬酰胺（N454） MLV起源于SEQ ID NO： ID。 NO：1与其他氨基酸。 与野生型逆转录酶相比，本发明的突变型逆转录酶表现出优异的热稳定性。 因此，即使在能够在高温下形成稳定的二级结构的RNA的存在下，也可以获得具有稳定的逆转录活性的靶cDNA。