公开(公告)号：US20140088578A1

公开(公告)日：2014-03-27

申请号：US14035866

申请日：2013-09-24

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Boris Rubinsky ,  Charlotte Daniels ,  Yair Granot ,  Liel Rubinsky

IPC: A61B18/02

CPC classification number: A61B18/02 ,  A61B18/1477 ,  A61B2018/00577 ,  A61B2018/00994 ,  A61B2018/0293

Abstract: Systems and methods are disclosed for treating a target volume of biological matter, by cooling a volume of tissue to a temperature below freezing, and directing an electric field through the cooled volume of tissue or tissue adjacent to the cooled volume of tissue to generate at least a temporary physiological affect on one or more of the cooled volume of tissue and adjacent volume of tissue. The generated physiological affect may include shielding a region of tissue from treatment, focusing treatment on a particular region of tissue, and sterilization of tissue.

Abstract translation: 公开了用于通过将组织体积冷却到低于冰点的温度来处理目标体积的生物物质的系统和方法，以及引导电场通过与冷却的组织体积相邻的组织或组织的冷却体积，以产生至少 对组织的冷却体积和组织的相邻体积的一个或多个产生暂时的生理影响。 产生的生理影响可以包括屏蔽组织区域的治疗，聚焦治疗对组织的特定区域以及组织的灭菌。

公开(公告)号：US20220287583A1

公开(公告)日：2022-09-15

申请号：US17479640

申请日：2021-09-20

Applicant: The Regents of the University of California

Inventor： Boris Rubinsky ,  Cesar A. GONZALEZ

IPC: A61B5/0537 ,  A61B5/05 ,  A61B5/00 ,  A61B5/053 ,  G01N27/02 ,  A61B5/02 ,  A61B5/0205 ,  G01R33/34 ,  G01R33/36 ,  G01R33/48 ,  G01R33/483

Abstract: A method and apparatus of determining the condition of a bulk tissue sample, by: positioning a bulk tissue sample between a pair of induction coils (or antennae); passing a spectrum of alternating current (or voltage) through a first of the induction coils (or antennae); measuring spectrum of alternating current (or voltage) produced in the second of the induction coils (or antennae); and comparing the phase shift between the spectrum of alternating currents (or voltages) in the first and second induction coils (or antennae), thereby determining the condition of the bulk tissue sample.

公开(公告)号：US20210137153A1

公开(公告)日：2021-05-13

申请号：US17253561

申请日：2019-06-28

Applicant: The Regents of the University of California

Inventor： Dan Rubinsky ,  Boris Rubinsky ,  Ze'ev Shaked ,  Gideon Ukpai

IPC: A23P20/20 ,  A23L33/00 ,  B33Y10/00 ,  B33Y40/10 ,  B33Y30/00 ,  B33Y70/00

Abstract: A method of additive manufacturing biological matter is provided. The method includes preparing an aqueous solution, combining the aqueous solution with a thickening gent, forming the combination into a plurality of two-dimensional individual volume elements in parallel, assembling the plurality of individual volume elements in a three-dimensional array and solidifying the three-dimensional array. Methods of additive manufacturing a food product and a three-dimensional structure with aqueous solution or organic matter are also provided. A system for additively depositing elements including an aqueous solution or organic matter is also provided.

公开(公告)号：US20170303991A1

公开(公告)日：2017-10-26

申请号：US15504522

申请日：2015-08-27

Applicant: The Regents of the University of California

Inventor： Boris Rubinsky ,  Arie Meir ,  Mohammad Hjouj

IPC: A61B18/14 ,  A61B5/145 ,  G01R33/48 ,  A61F9/007 ,  A61B5/053 ,  A61B5/055 ,  A61B18/00

CPC classification number: A61B18/1402 ,  A61B5/053 ,  A61B5/0536 ,  A61B5/055 ,  A61B5/14539 ,  A61B2018/00577 ,  A61B2018/00642 ,  A61B2018/00875 ,  A61B2018/00982 ,  A61B2018/1467 ,  A61B2562/0217 ,  A61F9/007 ,  G01R33/4804 ,  G01R33/4808

Abstract: Methods and compositions are provided for monitoring and optimizing electrolysis, for example, tissue electrolysis. Aspects of the methods include monitoring electrolysis of a tissue in a subject using an imaging technique or a measurement technique, e.g., a bulk spectroscopic measurement technique. Imaging techniques of interest include electrical impedance-based tomography and magnetic electrical impedance tomography. Electrical impedance-based imaging methods include imaging the electrical impedance of a tissue of the subject undergoing electrolysis, and monitoring the electrolysis based on one or more electrical impedance images of the tissue. Another modality to monitor electrolysis is by magnetic resonance imaging (MRI)-based methods which include imaging pH changes in a tissue of the subject undergoing electrolysis by magnetic resonance imaging, and monitoring the electrolysis based on one or more magnetic resonance images of the pH changes in the tissue. Measurement techniques of interest include bulk measurements of electrical properties and their changes with electrolysis or bulk changes in magnetic resonance readings and their changes with electrolysis. Devices and systems thereof that find use in practicing the methods are also provided.

公开(公告)号：US20160183840A1

公开(公告)日：2016-06-30

申请号：US14805057

申请日：2015-07-21

Applicant: The Regents of the University of California

Inventor： Boris Rubinsky ,  Cesar A. Gonzalez

IPC: A61B5/053 ,  A61B5/00

CPC classification number: A61B5/0537 ,  A61B5/02042 ,  A61B5/0205 ,  A61B5/05 ,  A61B5/053 ,  A61B5/4064 ,  A61B5/413 ,  A61B5/4244 ,  A61B5/443 ,  A61B5/4519 ,  A61B5/4869 ,  A61B5/4878 ,  A61B5/7225 ,  A61B5/7228 ,  A61B5/725 ,  A61B2562/143 ,  G01N27/026 ,  G01R33/34 ,  G01R33/36 ,  G01R33/4828 ,  G01R33/483

Abstract: A method and apparatus of determining the condition of a bulk tissue sample, by: positioning a bulk tissue sample between a pair of induction coils (or antennae); passing a spectrum of alternating current (or voltage) through a first of the induction coils (or antennae); measuring spectrum of alternating current (or voltage) produced in the second of the induction coils (or antennae); and comparing the phase shift between the spectrum of alternating currents (or voltages) in the first and second induction coils (or antennae), thereby determining the condition of the bulk tissue sample.

公开(公告)号：US12270601B2

公开(公告)日：2025-04-08

申请号：US17845114

申请日：2022-06-21

Applicant: The Regents of the University of California

Inventor： Matthew J. Powell-Palm ,  Boris Rubinsky

IPC: F25D3/08

Abstract: Supercooled water or solutions can be destabilized by the nucleation of ice. Constraining water in a constant-volume (isochoric) container minimizes destabilization through thermodynamic, kinetic, and fluid dynamic means, significantly enhancing supercooling stability. Methods and devices are used for stable and transportable supercooling of aqueous solutions or suspensions, and the stable and long-term preservation of biological matter that may be stored therein.

公开(公告)号：US20230284670A1

公开(公告)日：2023-09-14

申请号：US18167292

申请日：2023-02-10

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Boris Rubinsky ,  Dan Rubinsky ,  Gideon Ukpai

IPC: A23P20/25

CPC classification number: A23P20/25 ,  B33Y10/00

Abstract: Systems and methods for additive manufacturing of biological matter with desired non-homogeneous and non-isotropic textures from deposited 2-D or 3-D printed elements. Desired textures, such as anisotropic structure at the microscale level, are achieved through a combination of controlled chemical, thermal and freezing steps producing crosslinked anisotropic structures by directional solidification. The apparatus has a movable printing platform associated with a heating module and a cooling module separated by a gap that creates a thermal gradient permitting directional solidification of a printed object as the platform moves over the modules.

公开(公告)号：US11584066B2

公开(公告)日：2023-02-21

申请号：US17459717

申请日：2021-08-27

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Boris Rubinsky ,  Michal Adamkievicz ,  Ze'ev Shaked

IPC: B29C64/118 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y50/02 ,  B29C64/364 ,  B33Y40/20 ,  B33Y40/00 ,  B33Y70/00 ,  B29C64/379 ,  B29C64/209 ,  B29C64/393 ,  B29C64/25 ,  B29C64/371 ,  C12M3/00 ,  C12N5/00

Abstract: Systems, apparatus and methods for producing objects with cryogenic 3D printing with controllable micro and macrostructure with potential applications in tissue engineering, drug delivery, and the food industry. The technology can produce complex structures with controlled morphology when the printed 3D object is immersed in a liquid coolant, whose upper surface is maintained at the same level as the highest deposited layer of the object. This ensures that the computer-controlled process of freezing is controlled precisely and already printed frozen layers remain at a constant temperature. The technology controls the temperature, flow rate and volume of the printed fluid emitted by the dispenser that has X-Y positional translation and conditions at the interface between the dispenser and coolant surface. The technology can also control the temperature of the pool of liquid coolant and the vertical position of the printing surface and pool of coolant liquid.

公开(公告)号：US20200260986A1

公开(公告)日：2020-08-20

申请号：US16800958

申请日：2020-02-25

Applicant: The Regents of the University of California

Inventor： Boris Rubinsky ,  Cesar A. GONZALEZ

IPC: A61B5/053 ,  A61B5/00 ,  G01R33/483 ,  G01R33/48 ,  G01R33/36 ,  G01R33/34 ,  A61B5/0205 ,  A61B5/02 ,  G01N27/02 ,  A61B5/05

Abstract: A method and apparatus of determining the condition of a bulk tissue sample, by: positioning a bulk tissue sample between a pair of induction coils (or antennae); passing a spectrum of alternating current (or voltage) through a first of the induction coils (or antennae); measuring spectrum of alternating current (or voltage) produced in the second of the induction coils (or antennae); and comparing the phase shift between the spectrum of alternating currents (or voltages) in the first and second induction coils (or antennae), thereby determining the condition of the bulk tissue sample.

公开(公告)号：US20150094549A1

公开(公告)日：2015-04-02

申请号：US14507492

申请日：2014-10-06

Applicant: The Regents of the University of California

Inventor： Boris Rubinsky ,  Cesar A. Gonzalez

IPC: A61B5/00 ,  G01R33/36 ,  A61B5/0205 ,  G01R33/48 ,  A61B5/02 ,  G01R33/34 ,  G01R33/483

CPC classification number: A61B5/0537 ,  A61B5/02042 ,  A61B5/0205 ,  A61B5/05 ,  A61B5/053 ,  A61B5/4064 ,  A61B5/413 ,  A61B5/4244 ,  A61B5/443 ,  A61B5/4519 ,  A61B5/4869 ,  A61B5/4878 ,  A61B5/7225 ,  A61B5/7228 ,  A61B5/725 ,  A61B2562/143 ,  G01N27/026 ,  G01R33/34 ,  G01R33/36 ,  G01R33/4828 ,  G01R33/483

Abstract: A method and apparatus of determining the condition of a bulk tissue sample, by: positioning a bulk tissue sample between a pair of induction coils (or antennae); passing a spectrum of alternating current (or voltage) through a first of the induction coils (or antennae); measuring spectrum of alternating current (or voltage) produced in the second of the induction coils (or antennae); and comparing the phase shift between the spectrum of alternating currents (or voltages) in the first and second induction coils (or antennae), thereby determining the condition of the bulk tissue sample.

Abstract translation: 通过以下步骤来确定体组织样本的状况的方法和装置：将体组织样本定位在一对感应线圈（或天线）之间; 通过感应线圈（或天线）中的第一个通过交流（或电压）的频谱; 测量第二个感应线圈（或天线）中产生的交流（或电压）的频谱; 以及比较第一和第二感应线圈（或天线）中的交流电流（或电压）的频谱之间的相移，由此确定体组织样本的状况。