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公开(公告)号：US20070218478A1
公开(公告)日：2007-09-20
申请号：US11612454
申请日：2006-12-18
申请人： Qing Bai , Susana Salceda , Kai Wu , Thong Nguyen , Charles Miyada
发明人： Qing Bai , Susana Salceda , Kai Wu , Thong Nguyen , Charles Miyada
CPC分类号： C12Q1/6806 , C12Q2521/531 , C12Q2521/301 , C12Q2521/131
摘要： Methods for fragmenting and labeling DNA in a single reaction volume and incubation step using a uracil DNA glycosylase, an apurinic/apyrimidinic endonuclease, and a terminal transferase are disclosed. In a preferred embodiment the UDG, AP and TdT activities are first mixed together to form an enzyme mixture and then the enzyme mixture is mixed with the uracil containing DNA. The fragmentation and labeling reactions thus take place simultaneously as part of the same reaction. The methods may be used in a variety of applications where fragmenting and end-labeling single or double stranded DNA is desired.
摘要翻译： 公开了在单个反应体积中分离和标记DNA的方法以及使用尿嘧啶DNA糖基化酶，无嘌呤/脱嘧啶核苷酸内切酶和末端转移酶的温育步骤。 在优选的实施方案中，首先将UDG，AP和TdT活性混合在一起以形成酶混合物，然后将该酶混合物与含有尿嘧啶的DNA混合。 因此，碎裂和标记反应同时作为相同反应的一部分进行。 该方法可用于需要片段化和末端标记单链或双链DNA的多种应用。
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公开(公告)号：US20070075402A1
公开(公告)日：2007-04-05
申请号：US11243569
申请日：2005-10-05
申请人： Frank Geefay , David Dutton , Qing Bai
发明人： Frank Geefay , David Dutton , Qing Bai
IPC分类号： H01L29/40
CPC分类号： B81C1/00269 , B81C2203/019 , H01L23/10 , H01L23/3142 , H01L24/29 , H01L24/32 , H01L24/94 , H01L25/0657 , H01L25/50 , H01L2224/29 , H01L2224/29101 , H01L2224/29298 , H01L2224/83365 , H01L2224/83385 , H01L2225/06575 , H01L2924/00011 , H01L2924/00013 , H01L2924/01006 , H01L2924/01011 , H01L2924/01023 , H01L2924/01033 , H01L2924/01082 , H01L2924/0132 , H01L2924/01322 , H01L2924/014 , H01L2924/09701 , H01L2924/10253 , H01L2924/10329 , H01L2924/12044 , H01L2924/14 , H01L2924/00 , H01L2924/01031 , H01L2924/3512 , H01L2224/29099 , H01L2224/29199 , H01L2224/29299 , H01L2224/2929
摘要： An attachment system. The attachment system includes a first structure and a second structure. The first structure has a surface and a recess in the surface. The second structure is molded into the recess and extends above the surface. The second structure adheres to the first structure at a boundary of the recess.
摘要翻译： 附件系统。 附件系统包括第一结构和第二结构。 第一结构在表面具有表面和凹部。 第二结构被模制成凹槽并在表面上方延伸。 第二结构在凹槽的边界附着于第一结构。
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公开(公告)号：US20050145053A1
公开(公告)日：2005-07-07
申请号：US10752183
申请日：2004-01-07
申请人： Qing Bai , Storrs Hoen , Jonah Harley , Kirt Williams
发明人： Qing Bai , Storrs Hoen , Jonah Harley , Kirt Williams
CPC分类号： G02B26/0841 , B81B3/0062 , B81B2201/045 , Y10T74/20
摘要： A device includes first and second supports, a rotatable body and first and second flexible members. The first flexible member extends between the first support and a first position on the rotatable body. The second flexible member extends between the second support and a second position on the rotatable body. At least one of the supports is capable of linear movement in a first direction with respect to the other. The first position is offset from the second position in a second direction orthogonal to the first direction.
摘要翻译： 一种装置包括第一和第二支撑件，可旋转主体和第一和第二柔性构件。 第一柔性构件在第一支撑件和可旋转主体上的第一位置之间延伸。 第二柔性构件在第二支撑件和可旋转主体上的第二位置之间延伸。 至少一个支撑件能够相对于另一个在第一方向上线性移动。 第一位置在与第一方向正交的第二方向上偏离第二位置。
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公开(公告)号：US07615833B2
公开(公告)日：2009-11-10
申请号：US10890343
申请日：2004-07-13
申请人： John D. Larson, III , Qing Bai , Farid Matta , Tracy Verhoeven
发明人： John D. Larson, III , Qing Bai , Farid Matta , Tracy Verhoeven
IPC分类号： H01L41/00
CPC分类号： H03H9/105 , H03B5/326 , H03H3/02 , H03H9/0547 , H03H9/706
摘要： A microfabricated device has a first substrate, a second substrate, a film bulk acoustic resonator (FBAR) device, and a circuit. The second substrate is bonded to the first substrate to define a chamber. The FBAR device is located on a surface of the first substrate and inside the chamber. The circuit is located on a surface of the second substrate and inside the chamber. An electrical connection connects the circuit and the FBAR device.
摘要翻译： 微加工装置具有第一基板，第二基板，薄膜体声波谐振器（FBAR）装置和电路。 第二基板被结合到第一基板以限定室。 FBAR装置位于第一基板的表面和室内。 电路位于第二基板的表面和室内。 电气连接连接电路和FBAR设备。
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公开(公告)号：US07554177B2
公开(公告)日：2009-06-30
申请号：US11243569
申请日：2005-10-05
申请人： Frank S. Geefay , David T. Dutton , Qing Bai
发明人： Frank S. Geefay , David T. Dutton , Qing Bai
CPC分类号： B81C1/00269 , B81C2203/019 , H01L23/10 , H01L23/3142 , H01L24/29 , H01L24/32 , H01L24/94 , H01L25/0657 , H01L25/50 , H01L2224/29 , H01L2224/29101 , H01L2224/29298 , H01L2224/83365 , H01L2224/83385 , H01L2225/06575 , H01L2924/00011 , H01L2924/00013 , H01L2924/01006 , H01L2924/01011 , H01L2924/01023 , H01L2924/01033 , H01L2924/01082 , H01L2924/0132 , H01L2924/01322 , H01L2924/014 , H01L2924/09701 , H01L2924/10253 , H01L2924/10329 , H01L2924/12044 , H01L2924/14 , H01L2924/00 , H01L2924/01031 , H01L2924/3512 , H01L2224/29099 , H01L2224/29199 , H01L2224/29299 , H01L2224/2929
摘要： An attachment system. The attachment system includes a first structure and a second structure. The first structure has a surface and a recess in the surface. The second structure is molded into the recess and extends above the surface. The second structure adheres to the first structure at a boundary of the recess.
摘要翻译： 附件系统。 附件系统包括第一结构和第二结构。 第一结构在表面具有表面和凹部。 第二结构被模制成凹槽并在表面上方延伸。 第二结构在凹槽的边界附着于第一结构。
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公开(公告)号：US20050003392A1
公开(公告)日：2005-01-06
申请号：US10805559
申请日：2004-03-19
申请人： Susana Salceda , Kai Wu , Natalia Briones , Qing Bai , Yanxiang Cao
发明人： Susana Salceda , Kai Wu , Natalia Briones , Qing Bai , Yanxiang Cao
摘要： The present invention relates to the amplification of nucleic acids, preferably from mRNA. A primer and promoter are added to a target sequence to be amplified and then the target is amplified in an in vitro transcription reaction and the product of this reaction is used as template for subsequent rounds of amplification. Polyadenylated control transcripts are added to the nucleic acid sample prior to the first step of amplification to monitor the efficiency of the amplification and labeling reactions.
摘要翻译： 本发明涉及核酸，优选mRNA的扩增。 将引物和启动子加入待扩增的靶序列中，然后在体外转录反应中扩增靶，并将该反应的产物用作随后的扩增循环。 将多腺苷酸化的对照转录物在扩增的第一步骤之前加入到核酸样品中，以监测扩增和标记反应的效率。
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