公开(公告)号：US20120141831A1

公开(公告)日：2012-06-07

申请号：US13203896

申请日：2010-10-29

申请人： Wei Luo ,  Yoshio Goto ,  Seiji Yamada ,  Yuichi Tokita

发明人： Wei Luo ,  Yoshio Goto ,  Seiji Yamada ,  Yuichi Tokita

IPC分类号： H01L31/10 ,  C07K17/02

CPC分类号： H01L51/0093 ,  B82Y10/00 ,  C07K17/14 ,  H01L27/302 ,  H01L51/42 ,  Y02E10/549

摘要： A multilayer transparent light-receiving device with significantly high photoresponsive speed that is easily manufactured, and a high-performance electronic device using the multilayer transparent light-receiving device are provided. The multilayer transparent light-receiving device is composed by laminating a plurality of protein transparent light-receiving elements using an electron transfer protein. The protein transparent light-receiving element has a structure in which a transparent substrate, a transparent electrode, an electron transfer protein layer, an electrolyte layer, and a transparent counter electrode are sequentially laminated. The multilayer transparent light-receiving device is used as a light-receiving device for a camera, an optical disc system and the like.

摘要翻译： 提供了容易制造具有明显高的光响应速度的多层透明光接收装置，以及使用该多层透明光接收装置的高性能电子装置。 多层透明光接收装置通过使用电子转移蛋白层叠多个蛋白质透明的光接收元件而构成。 蛋白质透明受光元件具有依次层叠透明基板，透明电极，电子转移蛋白质层，电解质层和透明对置电极的结构。 多层透明光接收装置用作相机的光接收装置，光盘系统等。

公开(公告)号：US20120138770A1

公开(公告)日：2012-06-07

申请号：US13389759

申请日：2010-08-09

申请人： Wei Luo ,  Seiji Yamada ,  Yoshio Goto ,  Yuichi Tokita

发明人： Wei Luo ,  Seiji Yamada ,  Yoshio Goto ,  Yuichi Tokita

IPC分类号： G01J1/42 ,  B05D5/12

CPC分类号： H01L51/0093 ,  B82Y10/00 ,  H01L51/4206 ,  H01L51/4253 ,  Y02E10/542 ,  Y02P70/521

摘要： A non-wetted all solid protein photoelectric conversion device that is able to be operated without existence of a liquid such as water inside and outside of the device and a method of manufacturing the same are provided. The non-wetted all solid protein photoelectric conversion device has a structure in which a solid protein layer composed of an electron transfer protein is sandwiched between an electrode and an electrode. The solid protein layer is immobilized onto the electrodes and. The solid protein layer does not contain a liquid such as water. The solid protein layer is composed of a monomolecular film or a multimolecular film of the electron transfer protein.

摘要翻译： 提供能够在不存在装置内部和外部的水等液体的情况下操作的非湿润全固态蛋白质光电转换装置及其制造方法。 非湿润全固体蛋白质光电转换装置具有将由电子转移蛋白构成的固体蛋白质层夹在电极和电极之间的结构。 将固体蛋白质层固定在电极上。 固体蛋白质层不含液体如水。 固体蛋白质层由单分子膜或电子转移蛋白质的多分子膜组成。

公开(公告)号：US20120012823A1

公开(公告)日：2012-01-19

申请号：US13148548

申请日：2010-02-08

申请人： Seiji Yamada ,  Yuichi Tokita ,  Yoshio Goto ,  Wei Luo ,  Daisuke Yamaguchi ,  Daisuke Ito ,  Jusuke Shimura

发明人： Seiji Yamada ,  Yuichi Tokita ,  Yoshio Goto ,  Wei Luo ,  Daisuke Yamaguchi ,  Daisuke Ito ,  Jusuke Shimura

IPC分类号： H01L51/48 ,  H01L51/46 ,  C07K19/00 ,  B32B15/04 ,  C07K14/80 ,  C07K1/107

CPC分类号： H01L51/0093 ,  B82Y10/00 ,  G01J1/58 ,  H01L27/307 ,  H01L51/0012 ,  H01L51/0051 ,  H01L51/0083 ,  H01L51/0092 ,  H01L51/0098 ,  H01L51/42 ,  Y10T428/31685 ,  Y10T428/31768

摘要： A color imaging element, a photosensor and a photoelectric transducer which use a protein and are capable of being stably used for a long time, and methods of manufacturing them are provided. A zinc-substituted cytochrome c552 is immobilized on a gold electrode with a self-assembled monolayer in between to form a blue-light photoelectric transducer. Alternatively, a cytochrome c552 is immobilized on a gold electrode with a self-assembled monolayer in between, and a fluorescent protein absorbing blue light is bonded to the cytochrome c552, thereby forming a blue-light photoelectric transducer. These photoelectric transducers each are used as a color imaging element or a blue-light photoelectric transducer of a photosensor.

摘要翻译： 提供使用蛋白质并能够长期稳定使用的彩色成像元件，光传感器和光电传感器及其制造方法。 将锌取代的细胞色素c552固定在具有自组装单层的金电极之间，形成蓝光光电传感器。 或者，细胞色素c552固定在金电极上，其间具有自组装单层，吸收蓝光的荧光蛋白质结合到细胞色素c552上，从而形成蓝光光电变换器。 这些光电传感器各自用作光电传感器的彩色成像元件或蓝光光电传感器。

公开(公告)号：US20110236771A1

公开(公告)日：2011-09-29

申请号：US13132257

申请日：2009-11-30

申请人： Daisuke Yamaguchi ,  Yoshio Goto ,  Seiji Yamada ,  Yuichi Tokita

发明人： Daisuke Yamaguchi ,  Yoshio Goto ,  Seiji Yamada ,  Yuichi Tokita

IPC分类号： C12N9/04 ,  H01M8/16

CPC分类号： H01M14/00 ,  C12N9/0006

摘要： The present disclosure provides a mutant gluconate dehydrogenase whose enzyme activity and/or heat resistance is a predetermined level or higher. The mutant gluconate dehydrogenase is made by an amino acid sequence obtained by deleting, substituting, adding or inserting one or plural amino acids in an amino acid sequence represented by sequence number 1. The mutant gluconate dehydrogenase displays enzyme activity which is equal to or higher than 120% of a wild-type gluconate dehydrogenase made by an amino acid sequence represented by sequence number 1, and/or displays residual enzyme activity after heat treatment under predetermined conditions, which is equal to or higher than 20% of the enzyme activity before the heat treatment.

摘要翻译： 本公开内容提供其酶活性和/或耐热性为预定水平或更高水平的突变体葡萄糖酸脱氢酶。 突变型葡萄糖酸脱氢酶由通过在序列号1所示的氨基酸序列中缺失，取代，添加或插入一个或多个氨基酸而获得的氨基酸序列制成。突变型葡萄糖酸脱氢酶显示等于或高于 120％由序列号1表示的氨基酸序列制备的野生型葡萄糖酸脱氢酶，和/或在预定条件下热处理后显示残留酶活性，其等于或高于前述酶活性的20％ 热处理。

公开(公告)号：US08603822B2

公开(公告)日：2013-12-10

申请号：US13013239

申请日：2011-01-25

申请人： Daisuke Yamaguchi ,  Seiji Yamada ,  Yoshio Goto ,  Yuichi Tokita

发明人： Daisuke Yamaguchi ,  Seiji Yamada ,  Yoshio Goto ,  Yuichi Tokita

IPC分类号： C12N15/00 ,  C12N9/04 ,  C12N1/20 ,  C12P21/06 ,  C12P21/04 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12N9/0006 ,  H01M8/16 ,  Y02E60/527

摘要： A method for designing a heat-resistant mutant enzyme, the method including the step of reducing a distance between the α4 helix and the α6 helix in a protein three-dimensional structure to become smaller than that of a wild type enzyme through deletion, replacement, addition, or insertion of one or several amino acids in the amino acid sequence of the wild type enzyme with respect to tyrosine-dependent short-chain dehydrogenase/reductase.

摘要翻译： 一种耐热突变酶的设计方法，其特征在于，将蛋白质三维结构中的α4螺旋和α6螺旋之间的距离减小到比野生型酶的缺失，置换， 相对于酪氨酸依赖性短链脱氢酶/还原酶，在野生型酶的氨基酸序列中添加或插入一个或几个氨基酸。

公开(公告)号：US20120103798A1

公开(公告)日：2012-05-03

申请号：US13139461

申请日：2009-12-10

申请人： Seiji Yamada ,  Yoshio Goto ,  Yuichi Tokita

发明人： Seiji Yamada ,  Yoshio Goto ,  Yuichi Tokita

IPC分类号： C25B11/04 ,  B05D1/18 ,  B05D5/00

CPC分类号： C07K17/14 ,  B82Y10/00 ,  G01N33/5438 ,  G01N2333/80 ,  G01N2610/00 ,  H01L51/0093 ,  H01L51/4206 ,  Y02E10/549 ,  Y02P70/521

摘要： In one example embodiment, a protein-immobilized electrode is stably used for long time. In one example embodiment, a method of manufacturing the protein-immobilized electrode includes immobilizing cytochrome c552 having high stability to a chemically-stable gold electrode while maintaining electron transfer capability of the cytochrome c552. In one example embodiment, a self-assembled monolayer is formed on a gold electrode by using hydrophobic thiol and hydrophilic thiol. By dipping the gold electrode on which the self-assembled monolayer is formed in a cytochrome c552 solution, a protein-immobilized electrode in which a cytochrome c552 is immobilized to the gold electrode with the self-assembled monolayer in between is produced.

摘要翻译： 在一个示例性实施方案中，固定化蛋白质的电极长时间稳定地使用。 在一个示例性实施方案中，制备蛋白质固定化电极的方法包括将化学稳定的金电极具有高稳定性的细胞色素c552固定，同时保持细胞色素c552的电子转移能力。 在一个示例性实施方案中，通过使用疏水性硫醇和亲水硫醇在金电极上形成自组装单层。 通过在细胞色素c552溶液中浸渍形成自组装单层的金电极，产生其中细胞色素c552被固定到金电极上的蛋白质固定电极，其间具有自组装单层。

公开(公告)号：US08609438B2

公开(公告)日：2013-12-17

申请号：US13139461

申请日：2009-12-10

申请人： Seiji Yamada ,  Yoshio Goto ,  Yuichi Tokita

发明人： Seiji Yamada ,  Yoshio Goto ,  Yuichi Tokita

IPC分类号： G01N33/544 ,  G01N33/551

CPC分类号： C07K17/14 ,  B82Y10/00 ,  G01N33/5438 ,  G01N2333/80 ,  G01N2610/00 ,  H01L51/0093 ,  H01L51/4206 ,  Y02E10/549 ,  Y02P70/521

摘要： In one example embodiment, a protein-immobilized electrode is stably used for long time. In one example embodiment, a method of manufacturing the protein-immobilized electrode includes immobilizing cytochrome c552 having high stability to a chemically-stable gold electrode while maintaining electron transfer capability of the cytochrome c552. In one example embodiment, a self-assembled monolayer is formed on a gold electrode by using hydrophobic thiol and hydrophilic thiol. By dipping the gold electrode on which the self-assembled monolayer is formed in a cytochrome c552 solution, a protein-immobilized electrode in which a cytochrome c552 is immobilized to the gold electrode with the self-assembled monolayer in between is produced.

摘要翻译： 在一个示例性实施方案中，固定化蛋白质的电极长时间稳定地使用。 在一个示例性实施方案中，制备蛋白质固定化电极的方法包括将化学稳定的金电极具有高稳定性的细胞色素c552固定，同时保持细胞色素c552的电子转移能力。 在一个示例性实施方案中，通过使用疏水性硫醇和亲水硫醇在金电极上形成自组装单层。 通过在细胞色素c552溶液中浸渍形成自组装单层的金电极，产生其中细胞色素c552被固定到金电极上的蛋白质固定电极，其间具有自组装单层。

公开(公告)号：US20110212503A1

公开(公告)日：2011-09-01

申请号：US13013239

申请日：2011-01-25

申请人： Daisuke Yamaguchi ,  Seiji Yamada ,  Yoshio Goto ,  Yuichi Tokita

发明人： Daisuke Yamaguchi ,  Seiji Yamada ,  Yoshio Goto ,  Yuichi Tokita

IPC分类号： C12N9/02

CPC分类号： C12N9/0006 ,  H01M8/16 ,  Y02E60/527

摘要： A method for designing a heat-resistant mutant enzyme, the method including the step of reducing a distance between the α4 helix and the α6 helix in a protein three-dimensional structure to become smaller than that of a wild type enzyme through deletion, replacement, addition, or insertion of one or several amino acids in the amino acid sequence of the wild type enzyme with respect to tyrosine-dependent short-chain dehydrogenase/reductase.

摘要翻译： 一种耐热突变型酶的设计方法，其特征在于，将蛋白质三维结构中的α4螺旋和α6螺旋之间的距离减小为比野生型酶的缺失，置换， 相对于酪氨酸依赖性短链脱氢酶/还原酶，在野生型酶的氨基酸序列中添加或插入一个或几个氨基酸。

公开(公告)号：US08945650B2

公开(公告)日：2015-02-03

申请号：US11814715

申请日：2006-10-06

申请人： Shinya Mizuguchi ,  Nobutaka Torii ,  Masakazu Komatsu ,  Yuji Sakatani ,  Seiji Yamada

发明人： Shinya Mizuguchi ,  Nobutaka Torii ,  Masakazu Komatsu ,  Yuji Sakatani ,  Seiji Yamada

IPC分类号： A21D10/04 ,  A21D8/06 ,  A21C5/00 ,  A21D13/00

CPC分类号： A21D8/06 ,  A21C5/006 ,  A21D13/40

摘要： There are provided a manufacturing method and a manufacturing apparatus for Baumkuchen for easily and properly suppressing shape distortion of the Baumkuchen caused in a manufacturing process thereof. In manufacturing the Baumkuchen by alternately repeating application of batter around the whole outer circumference of a spit and baking of the applied batter, the batter is scraped off outer circumferences of opposite end portions of the spit before baking only in early stage of manufacturing until the number of repetitions reaches a specified value. As a result, the number of cake layers formed at the opposite end portions of the Baumkuchen is reduced from that at the other portion and outside diameters of the opposite end portions are reduced from a normal outside diameter to thereby suppress partial increase in the outside diameter at the opposite end portions of the Baumkuchen caused by drooping of the batter at the end portions of the spit before baking.

摘要翻译： 提供了一种Baumkuchen的制造方法和制造装置，用于容易且适当地抑制在其制造过程中引起的Baumkuchen的形状变形。 在制作Baumkuchen时，通过交替重复涂抹面糊在整个外周的吐痰和烘烤所施用的面糊，面糊在烘烤前仅在制造的早期直到数字 的重复达到指定值。 结果，形成在Baumkuchen的相对端部处的滤饼层的数量比其他部分减少，并且相对端部的外径从正常外径减小，从而抑制外径的部分增加 在Baumkuchen的相对端部，由烘烤前的吐出物端部处的面糊下垂引起。

公开(公告)号：US07906219B2

公开(公告)日：2011-03-15

申请号：US10599293

申请日：2005-03-25

申请人： Masaki Ohara ,  Takanori Igarashi ,  Masaharu Sugiyama ,  Seiji Yamada ,  Kenichi Takahashi ,  Atsuo Mochizuki ,  Yoshitsugu Motoe ,  Akihisa Inoue ,  Hisamichi Kimura

发明人： Masaki Ohara ,  Takanori Igarashi ,  Masaharu Sugiyama ,  Seiji Yamada ,  Kenichi Takahashi ,  Atsuo Mochizuki ,  Yoshitsugu Motoe ,  Akihisa Inoue ,  Hisamichi Kimura

IPC分类号： B32B3/00 ,  B32B15/00 ,  B32B15/18 ,  C23C4/00 ,  C23C4/06 ,  C23C4/08

CPC分类号： C22C19/07 ,  B32B15/01 ,  C22C45/008 ,  C22F1/10 ,  C23C4/11 ,  C23C4/18 ,  C23C24/04 ,  C23C30/00 ,  Y10T428/12493 ,  Y10T428/12951 ,  Y10T428/12993 ,  Y10T428/1317 ,  Y10T428/24851 ,  Y10T428/24926 ,  Y10T428/249921 ,  Y10T428/249953

摘要： A metallic glass laminate of the present invention is characterized in that a metallic glass layer of amorphous phase is formed on the substrate surface, and there is no continuous pore (pinhole) through the metallic glass layer. The metallic glass laminate is preferably obtained by solidification and lamination of at least part of the metallic glass powder in the molten state or in the supercooled liquid state on the substrate surface. Because of the dense metallic glass layer of homogenous amorphous phase, the functionalities of metallic glass such as corrosion resistance and wear resistance can be satisfactorily provided. A thick and a large-area metallic glass layer can be formed. The metallic glass layer can also be formed into various shapes within the supercooled liquid temperature range. In addition, a metallic glass bulk can be obtained by removing the substrate. The metallic glass laminate and the metallic glass bulk are utilized for a fuel cell separator, a hydrogen separation membrane, a hydrogen sensor, a solder-corrosion resisting member, etc.

摘要翻译： 本发明的金属玻璃层叠体的特征在于，在基板表面上形成非晶相的金属玻璃层，通过金属玻璃层不存在连续的孔（针孔）。 金属玻璃层压体优选通过将熔融状态的金属玻璃粉末或基板表面上的过冷液态的至少一部分进行固化和层压而获得。 由于均匀非晶相的致密金属玻璃层，可以令人满意地提供金属玻璃的功能，例如耐腐蚀性和耐磨性。 可以形成厚的和大面积的金属玻璃层。 金属玻璃层也可以在过冷液体温度范围内形成各种形状。 此外，通过除去基板可以获得金属玻璃体。 金属玻璃层压体和金属玻璃体用于燃料电池隔板，氢分离膜，氢传感器，耐焊锡腐蚀构件等。