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24 条记录 (耗时 0.044 秒)

    Parallel proximity ligation event analysis
    16.
    发明授权
    Parallel proximity ligation event analysis 有权
    平行邻近结扎事件分析

    公开(公告)号：US08481698B2

    公开(公告)日：2013-07-09

    申请号：US12407547

    申请日：2009-03-19

    申请人： Erez Lieberman ,  Andreas Gnirke ,  Daniel Aird

    发明人： Erez Lieberman ,  Andreas Gnirke ,  Daniel Aird

    IPC分类号： C12N15/11 ,  C07H21/04

    CPC分类号： G01N33/5308 ,  G01N33/536 ,  G01N33/585

    摘要： The present invention describes compositions and methods showing that the spatial proximity of intracellular components may be related to their ability to cooperate in intracellular biochemical reactions. In some embodiments, the present invention contemplates a variety of nucleic acid barcoded binding partners capable of determining the spatial proximity of intracellular components as determined by ligation of their respective nucleotide barcodes. As such, an intracellular component contact map may be constructed to fingerprint specific physiological and/or pharmacological intracellular conditions.

    摘要翻译： 本发明描述了组合物和方法，其显示细胞内组分的空间接近可能与其在细胞内生化反应中协同作用的能力有关。 在一些实施方案中，本发明考虑了通过其各自核苷酸条形码的连接测定的能够测定细胞内组分的空间接近度的多种核酸条形码结合配偶体。 因此，可以构建细胞内组分接触图以指纹特异性生理和/或药理学细胞内条件。

    Parallel Proximity Ligation Event Analysis
    17.
    发明申请
    Parallel Proximity Ligation Event Analysis 有权
    并行接近事件分析

    公开(公告)号：US20100240101A1

    公开(公告)日：2010-09-23

    申请号：US12407547

    申请日：2009-03-19

    申请人： Erez Lieberman ,  Andreas Gnirke ,  Daniel Aird

    发明人： Erez Lieberman ,  Andreas Gnirke ,  Daniel Aird

    IPC分类号： C12P19/30 ,  C07K16/00 ,  C07H21/04

    CPC分类号： G01N33/5308 ,  G01N33/536 ,  G01N33/585

    摘要： The present invention describes compositions and methods showing that the spatial proximity of intracellular components may be related to their ability to cooperate in intracellular biochemical reactions. In some embodiments, the present invention contemplates a variety of nucleic acid barcoded binding partners capable of determining the spatial proximity of intracellular components as determined by ligation of their respective nucleotide barcodes. As such, an intracellular component contact map may be constructed to fingerprint specific physiological and/or pharmacological intracellular conditions.

    摘要翻译： 本发明描述了组合物和方法，其显示细胞内组分的空间接近可能与其在细胞内生化反应中协同作用的能力有关。 在一些实施方案中，本发明考虑了通过其各自核苷酸条形码的连接测定的能够测定细胞内组分的空间接近度的多种核酸条形码结合配偶体。 因此，可以构建细胞内组分接触图以指纹特异性生理和/或药理学细胞内条件。

    HIGH THROUGHPUT PAIRED-END SEQUENCING OF LARGE-INSERT CLONE LIBRARIES
    20.
    发明申请
    HIGH THROUGHPUT PAIRED-END SEQUENCING OF LARGE-INSERT CLONE LIBRARIES 审中-公开
    大插入式图书馆的高通量配对结果

    公开(公告)号：US20140228223A1

    公开(公告)日：2014-08-14

    申请号：US13697108

    申请日：2011-05-10

    申请人： Andreas Gnirke ,  Robert Nicol ,  Louise Williams ,  Maura T. Costello ,  Scott Steelman

    发明人： Andreas Gnirke ,  Robert Nicol ,  Louise Williams ,  Maura T. Costello ,  Scott Steelman

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6874 ,  C12N15/1027 ,  C12N15/1031 ,  C12N15/1093 ,  C12Q1/6869 ,  C12Q2535/122

    摘要： The present invention is related to genomic nucleotide sequencing. In particular, the invention describes a paired end sequencing method that improves the yield of long-distance genomic read pairs by constructing long-insert clone libraries (i.e., for example, a fosIll library or a fosCN library) and converting the long-insert clone library using inverse polymerase chain reaction amplification or shearing and recircularization of shortened fragments into a library of co-ligated clone-insert ends. The resultant jumping libraries are compatible with massively parallel sequencing techniques. The compositions and methods disclosed herein contemplate sequencing complex genomes as well as detecting chromosomal structural rearrangements.

    摘要翻译： 本发明涉及基因组核苷酸测序。 特别地，本发明描述了一种配对末端测序方法，其通过构建长插入克隆文库（例如，fosIll文库或fosCN文库）并转化长插入克隆来提高长距离基因组读取对的产量 文库使用反向聚合酶链反应扩增或剪切并将缩短的片段再循环到共连接的克隆插入末端的文库中。 所得到的跳转库与大规模并行测序技术相兼容。 本文公开的组合物和方法考虑了对复合基因组的测序以及检测染色体结构重排。