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公开(公告)号：US20070059736A1
公开(公告)日：2007-03-15
申请号：US11450366
申请日：2006-06-12
申请人： Hisao Saito , Yasuhiko Sasaki , Ryo Miyake
发明人： Hisao Saito , Yasuhiko Sasaki , Ryo Miyake
CPC分类号： B01L3/502715 , B01L2300/0816 , B01L2300/0867 , B01L2300/0887 , G01N1/2813 , G01N2001/2833
摘要： A bio-analysis chip which is convenient in use and can promptly detect microbes with high accuracy. The bio-analysis chip comprises a collection member for collecting microbes in the atmosphere with the microbes adhering to the collection member, and a substrate in the form of a thin plate on which the collection member is mounted. The substrate has a collection member receiving section in which the collection member is placed, and a plurality of reagent reservoirs in which reagents for treating and analyzing the microbes are stored. The plurality of reagent reservoirs are connected to the collection member receiving section.
摘要翻译： 一种生物分析芯片，使用方便，可以高精度地及时检测微生物。 生物分析芯片包括收集构件，用于在微生物粘附到收集构件的情况下收集大气中的微生物，以及薄板形式的基板，收集构件安装在该基板上。 基板具有收集部件收纳部，收纳部件被放置在该收集部件收纳部中，并存储有用于处理和分析微生物的试剂的多个试剂储存器。 多个试剂储存器连接到收集构件接收部分。
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公开(公告)号：US06925896B1
公开(公告)日：2005-08-09
申请号：US10293053
申请日：2002-11-13
申请人： Garrett D. Morton
发明人： Garrett D. Morton
CPC分类号： G01N1/02 , G01N2001/007 , G01N2001/028 , G01N2001/2833
摘要： A method for recovering evidence from the footwear of a crime scene investigator is provided, comprising placing an adhesive evidence recovery sheet at an entryway to a crime scene, causing the crime scene investigator to step thereon upon exiting the crime scene, and covering the evidence recovery sheet with a protective cover. In this manner, evidence inadvertently adhering to the footwear of the investigator is removed and preserved for further analysis. In accordance with the method of this invention, a device for recovering evidence from the footwear of an investigator is provided, comprising an adhesive evidence recovery sheet and a protective cover. Adhesive strips on an obverse surface of the evidence recovery sheet prevent inadvertent dislodgement of the sheet from the floor surface on which it is placed.
摘要翻译： 提供了一种从犯罪现场调查员的鞋类中回收证据的方法，包括将粘性证据恢复片放置在犯罪现场的入口处，导致犯罪现场调查员在退出犯罪现场后步入其上，并覆盖证据恢复 带有保护盖的板。 以这种方式，不经意地遵守调查员的鞋类的证据被移除并保存以供进一步分析。 根据本发明的方法，提供了一种用于从研究者的鞋类中回收证据的装置，包括粘性证据恢复片和保护罩。 证据恢复片的正面上的胶带防止纸张从其放置的地板表面上无意中脱落。
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公开(公告)号：US06639657B2
公开(公告)日：2003-10-28
申请号：US09121677
申请日：1998-07-23
IPC分类号： G01N100
CPC分类号： G01N1/2813 , G01N1/2806 , G01N1/286 , G01N2001/2833 , G01N2001/284 , G02B21/32
摘要： Systems and methods for laser capture microdissection are disclosed. An inverted microscope includes a translation stage joystick subsystem. The systems and methods provide the advantages of increased speed and much lower rates of contamination.
摘要翻译： 公开了用于激光捕获显微切割的系统和方法。 倒置显微镜包括平移台操纵杆子系统。 这些系统和方法提供了增加速度和低得多的污染率的优点。
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公开(公告)号：US20020178784A1
公开(公告)日：2002-12-05
申请号：US10124677
申请日：2002-04-17
IPC分类号： G01N021/00 , G01N029/02
CPC分类号： G01N29/222 , G01N1/2202 , G01N1/2214 , G01N1/2252 , G01N5/02 , G01N15/0656 , G01N29/036 , G01N2001/2833 , G01N2291/02408
摘要： A process to determine aerosol particles in gaseous samples, specifically in the exhaust of diesel motors, includes the at least indirect deposit of aerosol particles on a piezoelectric oscillating crystal and the determination of the oscillation parameters of the piezoelectrically stimulated oscillation of the crystal. In order to assure the required reproducibility reliably and independently of the type of aerosol particles, and thus to achieve a high quality of measurements, the piezoelectric crystal is charged to oscillation during less than half the period of deposit of aerosol particles.
摘要翻译： 确定气态样品中气溶胶颗粒（特别是柴油发动机排气）中的气溶胶颗粒的方法包括将气溶胶颗粒至少间接沉积在压电振荡晶体上，以及确定晶体的压电激发振荡的振荡参数。 为了可靠地且独立于气溶胶颗粒的类型确保所需的再现性，并且因此为了获得高质量的测量，压电晶体在不到一小部分气溶胶颗粒沉积期间被充电至振荡。
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公开(公告)号：US20020066321A1
公开(公告)日：2002-06-06
申请号：US10057530
申请日：2002-01-25
申请人： Graftech
发明人： Paul L. Lagraff , Robert T. Letarte
IPC分类号： G01N001/00
CPC分类号： G01N15/00 , G01N1/2208 , G01N1/24 , G01N2001/2223 , G01N2001/2833 , G01N2015/0046 , G01N2015/0261
摘要： A slit impaction sampling device is for collecting airborne contaminants for subsequent analysis, includes a base with a microscope slide disposed thereon. The microscopic slide has an adhesive media located thereon to assist in adhering airborne particles on the microscopic slide. The base has a top cap secured thereto. The top cap has an inlet opening formed therethrough. The inlet opening has an outer venturi section and an inner laminar section that directs the air flow through the inlet opening into contact with the adhesive media such that the airborne particles form an impaction trace thereon. The air then flows around the microscope slide into an outlet passage and to a vacuum source.
摘要翻译： 狭缝冲击取样装置用于收集空气污染物用于随后的分析，包括其上布置有显微镜载玻片的底座。 微观载片具有位于其上的粘合剂介质，以有助于将载气颗粒粘附在微观载玻片上。 底座具有固定到其上的顶盖。 顶盖具有通过其形成的入口开口。 入口开口具有外文丘里部分和内层片部分，其引导空气流通过入口与粘合剂介质接触，使得空气中的颗粒在其上形成冲击痕迹。 空气然后绕显微镜载玻片流入出口通道和真空源。
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公开(公告)号：US06355439B1
公开(公告)日：2002-03-12
申请号：US09787794
申请日：2001-03-23
申请人： Yeon Bo Chung , Choon Hong Hwang , Eun Young Kim
发明人： Yeon Bo Chung , Choon Hong Hwang , Eun Young Kim
IPC分类号： C12Q168
CPC分类号： G01N1/04 , C12N15/1006 , G01N2001/028 , G01N2001/2833 , Y10T428/14 , Y10T428/1457 , Y10T428/1471 , Y10T428/1476 , Y10T428/28 , Y10T428/2835
摘要： Provided is a method for obtaining human DNA for genetic analysis, by taking the epidermis of testee by means of an adhesive sheet, and by extracting DNA from the epidermis stuck on the adhesive sheet. Provided are also combined sheets for conveniently storing DNA and a kit for taking the epidermis and analyzing DNA. Along with the kits, the method allows DNA to be easily obtained and stably stored for a long period of time. In addition, both the identification and the DNA analysis of a testee can be conducted at the same time by taking epidermal scraps from the testee, along with a figured epidermal print.
摘要翻译： 本发明提供一种用于获得用于遗传分析的人DNA的方法，其通过用粘合片取得被检者的表皮，并且从粘贴在粘合片上的表皮提取DNA。 还提供了用于方便地存储DNA的组合片和用于摄取表皮和分析DNA的试剂盒。 与试剂盒一起，该方法可以容易地获得DNA并长期稳定地储存。 此外，受试者的鉴定和DNA分析可以同时通过从受试者身上取出表皮废料以及计算的表皮印记进行。
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公开(公告)号：US20020001074A1
公开(公告)日：2002-01-03
申请号：US09121691
申请日：1998-07-23
IPC分类号： G01N001/00 , G01N021/01
CPC分类号： G01N1/2813 , G01N1/2806 , G01N1/286 , G01N2001/2833 , G01N2001/284 , G02B21/32
摘要： Systems and methods for laser capture microdissection are disclosed. An inverted microscope includes a vacuum chuck subsystem that is adapted to hold a microscope slide down during the process of laser capture microdissection. The systems and methods provide the advantages of increased speed and much lower rates of contamination.
摘要翻译： 公开了用于激光捕获显微切割的系统和方法。 倒置显微镜包括在激光捕获显微切割过程中适于保持显微镜下滑的真空吸盘子系统。 这些系统和方法提供了增加速度和低得多的污染率的优点。
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公开(公告)号：US06204030B1
公开(公告)日：2001-03-20
申请号：US09388805
申请日：1999-09-02
IPC分类号： C12P1912
CPC分类号： G02B21/32 , C12M33/02 , G01N1/04 , G01N1/08 , G01N1/2806 , G01N1/2813 , G01N1/286 , G01N1/31 , G01N15/1468 , G01N33/50 , G01N2001/028 , G01N2001/2833 , G01N2001/284 , G01N2035/00237 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
摘要： A method of microdissection which involves: forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extraction is achieved by contacting the tissue sample with a transfer surface that can be selectively activated so that regions thereof adhere to the zone of cells of interest to be extracted. The transfer surface includes an activatable adhesive layer which provides chemical or electrostatic adherence to the selected regions of the tissue sample. After the transfer surface is activated the transfer surface and tissue sample are separated. During separation the zone of cells of interest remains adhered to the transfer surface and is thus separated from the tissue sample.
摘要翻译： 一种显微切割的方法，其包括：使用显微镜形成组织样品的细胞的图像场，从细胞的图像区域鉴定感兴趣的细胞的至少一个区域，所述细胞的至少一个区域包括不同类型的细胞 而不是相邻的细胞区域，并且从组织样品中提取感兴趣的细胞的至少一个区域。 通过使组织样品与可以选择性活化的转移表面接触使得其区域粘附到待提取的感兴趣的细胞区域来实现提取。 转移表面包括可激发的粘合剂层，其向组织样品的选定区域提供化学或静电粘附。 转移表面被激活后，分离转移表面和组织样品。 在分离期间，感兴趣的细胞区域保持粘附到转移表面，并因此与组织样品分离。
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公开(公告)号：US06184973B2
公开(公告)日：2001-02-06
申请号：US09058711
申请日：1998-04-10
IPC分类号： G01N100
CPC分类号： G01N1/2813 , G01N1/2806 , G01N1/286 , G01N2001/2833 , G01N2001/284 , G02B21/32
摘要： Systems and methods for laser capture microdissection are disclosed. A laser capture microdissection method includes: providing a sample that is to undergo laser capture microdissection; positioning the sample within an optical axis of a laser capture microdissection instrument; providing a transfer film carrier removably positioned in a transfer film carrier handling subsystem, the transfer film carrier having a substrate surface and a laser capture microdissection transfer film coupled to the substrate surface; and then placing the laser capture microdissection transfer film in juxtaposition with the sample by moving the transfer film carrier handling subsystem, the laser capture microdissection transfer film being placed in juxtaposition with a pressure sufficient to allow laser capture microdissection transfer of a portion of the sample to the laser capture microdissection transfer film, without forcing nonspecific transfer of a remainder of the sample to the laser capture microdisection film; and then transferring a portion of the sample to the laser capture microdissection transfer film, without forcing nonspecific transfer of a remainder of the sample to the laser capture microdissection transfer film. Transferring includes moving the laser capture microdissection transfer film and the portion of the sample away from the remainder of the sample with the transfer film carrier handling subsystem. The transfer film carrier handling subsystem can be mounted on an inverted microscope. The systems and methods provide the advantages of increased speed and much lower rates of contamination
摘要翻译： 公开了用于激光捕获显微切割的系统和方法。 激光捕获显微切割方法包括：提供要进行激光捕获显微切割的样品; 将样品定位在激光捕获显微切割仪器的光轴内; 提供可移除地定位在转印膜载体处理子系统中的转印膜载体，所述转印膜载体具有耦合到所述基板表面的基板表面和激光捕获显微切割转印膜; 然后通过移动转印膜载体处理子系统将激光捕获显微切割转印膜与样品并置放置，激光捕获显微切割转印膜以足以允许激光捕获将样品的一部分的显微切割转移到 激光捕获显微切割转移膜，而不强制将其余样品的非特异性转移到激光捕获微分解膜; 然后将样品的一部分转移到激光捕获显微切割转移膜，而不会将剩余的样品的非特异性转移到激光捕获显微切割转移膜。 转移包括使用转印膜载体处理子系统将激光捕获显微切割转移膜和样品的一部分移离样品的其余部分。 转印膜载体处理子系统可以安装在倒置显微镜上。 这些系统和方法提供了增加速度和低得多的污染率的优点
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公开(公告)号：US6100051A
公开(公告)日：2000-08-08
申请号：US883821
申请日：1997-06-27
CPC分类号： G02B21/34 , G01N1/2813 , G01N2001/2833 , G01N2001/284 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
摘要： A process of microdissection where a tissue sample is conventionally visualized in a microscope. A selectively activatable convex surface is provided, preferably on the periphery at the distal end of a rod. This selectively activatable convex surface when locally activated, typically with a laser through an optic light path in the microscope, provides the activated region with adhesive properties. The tissue sample has at least one portion, which is to be extracted is identified. This identified portion is contacted with a portion of the selectively activatable convex surface on the periphery of the rod. When the convex surface is locally activated, typically by exposure to laser light in the footprint of the desired portion, an adhesive transfer surface on the selectively activatable convex surface is activated which adheres to the desired cells in the footprint of the desired portion. Thereafter, the adhesive transfer surface is separated from the remainder of the tissue sample while maintaining adhesion with the portion of the sample. Thus the desired portion of the tissue sample is extracted. The disclosed selectively activatable convex surface is preferably utilized to collect desired tissue samples at more than one location on the same slide or from different slides. A rod having a convex surface with the selectively activatable material is set forth as a staple for use with the apparatus and process. Preferred shapes for the convex surface are disclosed as well as a method for coating rods.
摘要翻译： 显微切割的过程，其中组织样品通常在显微镜中可视化。 优选地，在棒的远端的周边上设置有选择性激活的凸面。 当局部激活时，这种选择性激活的凸表面通常在显微镜中通过光学光路通过激光提供具有粘合性质的激活区域。 组织样本具有至少一部分要被提取的部分。 该识别部分与杆的周边上的可选择性激活的凸面的一部分接触。 当凸表面被局部激活时，通常通过暴露在所需部分的覆盖区内的激光，激活可选择性激活的凸表面上的粘合剂转移表面，该粘合剂转移表面在期望部分的覆盖区中粘附到期望的电池。 此后，粘合剂转移表面与组织样品的其余部分分离，同时保持与样品部分的粘附。 因此，提取组织样本的期望部分。 所公开的可选择激活的凸表面优选用于在相同载片上或不同载玻片上的多于一个位置收集所需的组织样本。 具有具有可选择性活化材料的凸起表面的杆被设置为与装置和工艺一起使用的订书钉。 公开了用于凸表面的优选形状以及用于涂覆棒的方法。
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