公开(公告)号：US20110278492A1

公开(公告)日：2011-11-17

申请号：US13146543

申请日：2010-02-12

申请人： Naoki Arai ,  Jun Tomono ,  Shinichi Yokota ,  Yuji Tominaga

发明人： Naoki Arai ,  Jun Tomono ,  Shinichi Yokota ,  Yuji Tominaga

IPC分类号： C09K3/18

CPC分类号： A23L3/3472 ,  A23L19/00 ,  A23L19/03 ,  A23V2002/00 ,  A23V2200/206 ,  A23V2250/21

摘要： The objective of the present invention is to provide an antifreeze substance which is suitable for practical use and has excellent antifreeze activity in a safe process flow applicable to food production in a simple, efficient and inexpensive manner. The first method for producing a plant extract containing an antifreeze substance according to the present invention comprises the steps of drying a plant containing the antifreeze substance and extracting the antifreeze substance from the dried plant. The second method for producing a plant extract containing an antifreeze substance according to the present invention comprises the steps of extracting the antifreeze substance from a plant, and treating the obtained extract from the extracting step with an adsorbent.

摘要翻译： 本发明的目的是提供一种适用于实际应用的防冻物质，并且以简单，有效和廉价的方式在适用于食品生产的安全工艺流程中具有优异的防冻活性。 制备本发明含有防冻物质的植物提取物的第一种方法包括干燥含有防冻物质的植物并从干燥植物中提取防冻物质的步骤。 制备本发明含有防冻物质的植物提取物的第二种方法包括以下步骤：从植物中提取防冻物质，并用吸附剂处理所提取的提取物。

公开(公告)号：US07622291B2

公开(公告)日：2009-11-24

申请号：US11948650

申请日：2007-11-30

申请人： Jun Tomono ,  Hiroaki Sagawa ,  Ikunoshin Kato

发明人： Jun Tomono ,  Hiroaki Sagawa ,  Ikunoshin Kato

IPC分类号： C07H21/04 ,  C12N1/21 ,  C12N15/63 ,  C12P19/26 ,  C12N9/24 ,  C12N9/40

CPC分类号： C12Y302/01081 ,  C12N9/2468

摘要： Production of efficiently producing agaro-oligosaccharide; a polypeptide having an agarase activity which is usable in, for example, efficiently extracting a substance such as a nucleic acid from an agarose gel; the amino acid sequence of the polypeptide; a gene encoding the polypeptide; a process for producing the polypeptide; a process for producing agaro-oligosaccharide; and a process for extracting a substance such as a nucleic acid from an agarose gel.

摘要翻译： 高效生产琼脂寡糖的生产; 具有琼脂糖酶活性的多肽，其可用于例如从琼脂糖凝胶中有效地提取诸如核酸的物质; 多肽的氨基酸序列; 编码该多肽的基因; 一种生产该多肽的方法; 制备琼脂寡糖的方法; 以及从琼脂糖凝胶中提取核酸等物质的方法。

公开(公告)号：US20070148752A1

公开(公告)日：2007-06-28

申请号：US11681453

申请日：2007-03-02

申请人： Harumi Ueno ,  Jun Tomono ,  Hiroaki Sagawa ,  Takeshi Sakai ,  Ikunoshin Kato

发明人： Harumi Ueno ,  Jun Tomono ,  Hiroaki Sagawa ,  Takeshi Sakai ,  Ikunoshin Kato

IPC分类号： C12N9/24 ,  C12P21/06 ,  C12N1/21 ,  C12N15/74

CPC分类号： C12N9/2465 ,  C12N9/2402 ,  C12P19/00 ,  C12P19/04 ,  C12P19/12 ,  C12P19/14 ,  G01N2333/924

摘要： A gene encoding a polypeptide having an activity of digesting sulfated fucogalactan which is useful in sugar chain engineering reagents, analyzing the structures of sulfated fucose-containing polysaccharides and preparing degraded products of the polysaccharides; a genetic engineering process for producing the polypeptide; and the polypeptide obtained by the process.

摘要翻译： 编码具有消化硫酸化的赖氨酰半乳糖的活性的多肽的基因，其可用于糖链工程试剂，分析含硫酸岩藻糖多糖的结构并制备多糖的降解产物; 用于产生多肽的基因工程方法; 和通过该方法获得的多肽。

公开(公告)号：US20070037139A1

公开(公告)日：2007-02-15

申请号：US10555876

申请日：2004-05-07

申请人： Jun Tomono ,  Harumi Ueno ,  Osamu Takeda ,  Hiroyuki Mukai ,  Hiroaki Sagawa ,  Ikunoshin Kato

发明人： Jun Tomono ,  Harumi Ueno ,  Osamu Takeda ,  Hiroyuki Mukai ,  Hiroaki Sagawa ,  Ikunoshin Kato

IPC分类号： C12Q1/70 ,  C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6844 ,  C12Q2527/107

摘要： A method of analyzing, in chromosomal DNA having a retroviral vector incorporated therein, DNA region derived from chromosome which is adjacent to the vector, characterized in that a primer extension reaction and a nucleic acid amplification reaction are carried out in the presence of a compound capable of lowering the Tm value of double stranded nucleic acid. There are further provided a kit and buffer for use in this method.

摘要翻译： 一种在其中并入有逆转录病毒载体的染色体DNA中分析衍生自与载体相邻的染色体的DNA区域的方法，其特征在于引物延伸反应和核酸扩增反应在具有能力的化合物存在下进行 降低双链核酸的Tm值。 此外还提供了用于该方法的试剂盒和缓冲液。

公开(公告)号：US20060234355A1

公开(公告)日：2006-10-19

申请号：US10464488

申请日：2003-06-19

申请人： Jun Tomono ,  Yoshiko Nomura ,  Hiroaki Sagawa ,  Takeshi Sakai ,  Ikunoshin Kato

发明人： Jun Tomono ,  Yoshiko Nomura ,  Hiroaki Sagawa ,  Takeshi Sakai ,  Ikunoshin Kato

IPC分类号： C12P19/04 ,  C07H21/04 ,  C12P21/06 ,  C12N9/38 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12Y302/01158 ,  C12N9/2465 ,  C12P19/04 ,  C12P19/24

摘要： A polypeptide having an α-agarase activity, a gene encoding the polypeptide, a method for producing the polypeptide by genetic engineering and a method for producing an agarooligosaccharide using the polypeptide.

摘要翻译： 具有α-琼脂糖酶活性的多肽，编码该多肽的基因，通过遗传工程生产该多肽的方法以及使用该多肽制备琼脂低聚糖的方法。

公开(公告)号：US06951722B2

公开(公告)日：2005-10-04

申请号：US09935338

申请日：2001-08-23

申请人： Hiroyuki Mukai ,  Hiroaki Sagawa ,  Takashi Uemori ,  Junko Yamamoto ,  Jun Tomono ,  Eiji Kobayashi ,  Tatsuji Enoki ,  Osamu Takeda ,  Kazue Miyake ,  Yoshimi Sato ,  Mariko Moriyama ,  Haruhisa Sawaragi ,  Michio Hagiya ,  Kiyozo Asada ,  Ikunoshin Kato

发明人： Hiroyuki Mukai ,  Hiroaki Sagawa ,  Takashi Uemori ,  Junko Yamamoto ,  Jun Tomono ,  Eiji Kobayashi ,  Tatsuji Enoki ,  Osamu Takeda ,  Kazue Miyake ,  Yoshimi Sato ,  Mariko Moriyama ,  Haruhisa Sawaragi ,  Michio Hagiya ,  Kiyozo Asada ,  Ikunoshin Kato

IPC分类号： C07H21/04 ,  C12N9/22 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12N9/1252 ,  C12P19/34 ,  C12Q1/6853 ,  C12Q2531/119 ,  C12Q2525/121 ,  C12Q2521/301

摘要： A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.

公开(公告)号：US06599729B2

公开(公告)日：2003-07-29

申请号：US09924097

申请日：2001-08-08

申请人： Jun Tomono ,  Yoshiko Nomura ,  Hiroaki Sagawa ,  Takeshi Sakai ,  Ikunoshin Kato

发明人： Jun Tomono ,  Yoshiko Nomura ,  Hiroaki Sagawa ,  Takeshi Sakai ,  Ikunoshin Kato

IPC分类号： C12N924

CPC分类号： C12Y302/01158 ,  C12N9/2465 ,  C12P19/04 ,  C12P19/24

摘要： A polypeptide having an &agr;-agarase activity, a gene encoding the polypeptide, a method for producing the polypeptide by genetic engineering and a method for producing an agarooligosaccharide using the polypeptide.

公开(公告)号：US09097718B2

公开(公告)日：2015-08-04

申请号：US13823946

申请日：2011-09-21

申请人： Shigehiko Miyamoto ,  Takaaki Jikihara ,  Sotaro Sano ,  Koji Takahashi ,  Jun Tomono

发明人： Shigehiko Miyamoto ,  Takaaki Jikihara ,  Sotaro Sano ,  Koji Takahashi ,  Jun Tomono

IPC分类号： G01N33/58 ,  G01N21/78 ,  C12Q1/68

CPC分类号： G01N33/583 ,  C12Q1/6816 ,  C12Q1/6846 ,  C12Q1/686 ,  G01N21/78 ,  Y10T436/143333 ,  C12Q2563/173

摘要： The present invention provides a method for detecting a nucleic acid, by which a multi-stranded nucleic acid amplified by a nucleic acid amplification method is detected easily and with a high degree of accuracy without the need for specialized equipment, and also provides a nucleic acid detection device. Provided are a method for detecting a nucleic acid under visible light via a color reaction produced by contact between a chromogenic leuco dye and the multi-stranded nucleic acid, as well as a nucleic acid detection device using this method.

摘要翻译： 本发明提供一种检测核酸的方法，通过该方法可以容易地且高精度地检测通过核酸扩增方法扩增的多链核酸而不需要专门的设备，并且还提供核酸 检测装置 提供了通过由显色无色染料和多链核酸之间的接触产生的显色反应以及使用该方法的核酸检测装置在可见光下检测核酸的方法。

公开(公告)号：US20140065725A1

公开(公告)日：2014-03-06

申请号：US13989207

申请日：2011-11-24

申请人： Koji Takahashi ,  Shigehiko Miyamoto ,  Takaaki Jikihara ,  Jun Tomono

发明人： Koji Takahashi ,  Shigehiko Miyamoto ,  Takaaki Jikihara ,  Jun Tomono

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6834 ,  C12Q1/6832 ,  C12Q1/686 ,  G01N33/54313 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2565/519 ,  C12Q2525/186 ,  C12Q2531/113 ,  C12Q2565/625

摘要： An object of the invention is to provide a nucleic acid detection method which takes advantage of the high specificity of hybridization techniques, reduces the time length and the number of steps required for detection of PCR products, and allows for easy and highly accurate detection by visual observation without the need of special equipment; and a nucleic acid detection device or kit. The invention provides a method for detecting a target nucleic acid in a sample, which includes performing amplification of the target nucleic acid sequence to synthesize an amplification product having a partially double-stranded structure where a single-stranded region is added to each end of the target sequence, and hybridizing a nucleic acid sequence bound to a development medium and a nucleic acid sequence labeled with a labeling compound with the single-stranded regions of the amplification product to form a sandwich hybridization complex; and a detection device thereof.

摘要翻译： 本发明的目的是提供一种核酸检测方法，其利用杂交技术的高特异性，减少检测PCR产物所需的时间长度和数量，并且允许通过视觉方便和高度准确的检测 观察无需特殊设备; 和核酸检测装置或试剂盒。 本发明提供了一种用于检测样品中靶核酸的方法，其包括进行目标核酸序列的扩增以合成具有部分双链结构的扩增产物，其中单链区域被添加到 靶序列，并将与显影培养基结合的核酸序列与标记化合物标记的核酸序列与扩增产物的单链区域杂交以形成夹心杂交复合物; 及其检测装置。

公开(公告)号：US20130146803A1

公开(公告)日：2013-06-13

申请号：US13818637

申请日：2011-08-11

申请人： Hidehisa Kawahara ,  Yoshihide Koide ,  Naoki Arai ,  Jun Tomono

发明人： Hidehisa Kawahara ,  Yoshihide Koide ,  Naoki Arai ,  Jun Tomono

IPC分类号： C09K5/20

CPC分类号： C09K5/20 ,  A01N1/0221 ,  A23L3/3463 ,  A23L3/3472 ,  A23L5/00 ,  A23L15/20 ,  A23L15/30 ,  A23L17/00 ,  A23L31/00 ,  A61K8/73 ,  A61Q19/00 ,  C07K14/415 ,  C07K16/44

摘要： The objective to be solved by the present invention is to provide an ice-crystallization inhibitor which has a practicable and excellent ice-crystallization inhibiting property and which can be efficiently and stably produced in a safe process suitable for food production. Also, the objective of the present invention is to provide an antibody which specifically reacts with the ice-crystallization inhibitor, and a composition, a food, a biological sample protectant and a cosmetic which contain the ice-crystallization inhibitor. Furthermore, the objective of the present invention is to provide a polysaccharide which is derived from a basidiomycete and which is used for inhibiting ice-crystallization of a liquid containing water, and a method for inhibiting ice-crystallization of a liquid containing water. The ice-crystallization inhibitor according to the present invention is characterized in being a polysaccharide derived from a basidiomycete.

摘要翻译： 本发明要解决的目的是提供一种冰结晶抑制剂，其具有可行且优异的结晶结晶抑制性能，并且可以在适于食品生产的安全工艺中有效且稳定地生产。 此外，本发明的目的是提供与冰结晶抑制剂特异性反应的抗体，以及含有冰结晶抑制剂的组合物，食品，生物样品保护剂和化妆品。 此外，本发明的目的是提供一种源自担子菌的多糖，其用于抑制含水液体的冰结晶，以及抑制含有水的液体的冰结晶的方法。 根据本发明的结冰抑制剂的特征在于来自担子菌的多糖。