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公开(公告)号：US20160010107A1
公开(公告)日：2016-01-14
申请号：US14377843
申请日：2013-02-18
Applicant: Keygene N.V.
Inventor： Anne DESLATTES MAYS , Marieke Helena Adriana VAN HULTEN , Shital Anilkumar DIXIT , Martin DE VOS , Jesse David MUNKVOLD , Matthew Vitabile DILEO , Evert-Jan BLOM
CPC classification number: C12N15/8273 , A01H1/06 , G01N33/0098
Abstract: The present invention relates to a new method for increasing drought resistance of a plant. The method encompasses the impairment of the expression of a gene or genes in said plant. In comparison to a plant not manipulated to impair the expression of said gene(s), the plants display improved drought resistance. Also provided are plants and plant product that can be obtained by the method according to the invention.
Abstract translation: 本发明涉及一种提高植物耐旱性的新方法。 该方法包括所述植物中基因或基因表达的损伤。 与未被操作以损害所述基因表达的植物相比，植物表现出改善的抗旱性。 还提供了可以通过根据本发明的方法获得的植物和植物产品。
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公开(公告)号：US20150232924A1
公开(公告)日：2015-08-20
申请号：US14626822
申请日：2015-02-19
Applicant: Keygene N.V.
CPC classification number: G06F19/22 , C12N15/1065 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6858 , C12Q1/6874 , C12Q1/6883 , C12Q2600/156 , C40B30/04 , C40B30/10 , C12Q2539/107 , C12Q2531/113 , C12Q2525/191
Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.
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公开(公告)号：US20150191741A1
公开(公告)日：2015-07-09
申请号：US14640700
申请日：2015-03-06
Applicant: Keygene N.V.
Inventor： Paul BUNDOCK
CPC classification number: C12N15/8275 , C12N9/1092 , C12Y205/01019
Abstract: The present invention relates to a new method for improving glyphosate resistance of a plant. The method encompasses providing one or more specific mutations in a specific nucleotide sequence in said plant. In comparison to a plant not manipulated according to the method, the plant obtained by the method displays (improved) glyphosate resistance. Also provided are a (transgenic) plant, including a seed thereof, and a plant product that can be obtained by the method according to the invention.
Abstract translation: 本发明涉及一种提高植物抗草甘膦性的新方法。 该方法包括在所述植物中的特定核苷酸序列中提供一个或多个特异性突变。 与根据该方法未操作的植物相比，通过该方法获得的植物显示（改善）草甘膦抗性。 还提供了包含其种子的（转基因）植物，以及可以通过根据本发明的方法获得的植物产品。
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公开(公告)号：US09062348B1
公开(公告)日：2015-06-23
申请号：US14550805
申请日：2014-11-21
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia Van Eijk , Anker Preben Sørensen , Marco Gerardus Maria Van Schriek
CPC classification number: C12Q1/6874 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6876 , C12Q1/6883 , C12Q2600/156 , C12Q2600/16 , C12Q2565/301 , C12Q2535/138 , C12Q2535/122 , C12Q2527/107
Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.
Abstract translation: 本发明涉及一种用于一种或多种样品中一种或多种遗传标记物的高通量发现，检测和基因分型的方法，其包括DNA的限制性内切核酸酶消化，衔接子连接，任选的预扩增，选择性扩增，合并 的扩增产物，对文库以足够的冗余进行测序，聚类，然后鉴定文库内和/或文库之间的遗传标记和确定遗传标记的（共）优势基因型。
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公开(公告)号：US20150159217A1
公开(公告)日：2015-06-11
申请号：US14550805
申请日：2014-11-21
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia VAN EIJK , Anker Preben Sørensen , Marco Gerardus Maria Van Schriek
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6876 , C12Q1/6883 , C12Q2600/156 , C12Q2600/16 , C12Q2565/301 , C12Q2535/138 , C12Q2535/122 , C12Q2527/107
Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.
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Patent Agency Ranking
公开(公告)号：US20150101084A1
公开(公告)日：2015-04-09
申请号：US14571095
申请日：2014-12-15
Applicant: Keygene N.V.
CPC classification number: C12N15/8286 , A01H1/04 , C12N9/88 , C12N15/8239 , C12N15/8243 , C12P5/007 , C12Y402/00 , C12Y402/03053 , C12Y402/03054 , C12Y402/03082 , Y02A40/162
Abstract: The disclosure provides an isolated nucleic acid molecule encoding a 7-epizingiberene synthase, a chimeric gene comprising said nucleic acid molecule, vectors comprising the same, as well as isolated 7-epizingiberene synthase proteins themselves. In addition, transgenic plants and plant cells comprising a gene encoding a 7-epizingiberene synthase, optionally integrated in its genome, and methods for making such plants and cells, are provided. Especially Solanaceae plants and plant parts (seeds, fruit, leaves, etc.) with enhanced insect pest resistance are provided.
Abstract translation: 本公开提供了分离的编码7-表位活性成分合成酶的核酸分子，包含所述核酸分子的嵌合基因，包含该核酸分子的载体以及分离的7-表质酰基合成酶本身。 此外，提供了转基因植物和植物细胞，其包含编码7-表位活性物质合成酶的基因，任选地整合在其基因组中，以及用于制备这种植物和细胞的方法。 提供具有增强的害虫抗性的茄科植物和植物部分（种子，水果，叶子等）。
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27.发明授权公开(公告)号：US08685650B2
公开(公告)日：2014-04-01
申请号：US13783601
申请日：2013-03-04
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia Van Eijk , Taco Peter Jesse
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2535/138
Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3′ end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.
Abstract translation: 本发明涉及用于识别和检测其中产生限制性片段的分子标记物的高通量方法，并且包括（样品特异性）标识符的合适的衔接子被连接。 衔接子连接的限制性片段可以用在3'末端携带选择性核苷酸的衔接子兼容引物进行选择性扩增。 使用高通量测序方法至少部分地测序扩增的接头连接的限制性片段，并且限制性片段的序列部分与样品特异性标识符一起用作分子标记。
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公开(公告)号：US12203080B2
公开(公告)日：2025-01-21
申请号：US17142063
申请日：2021-01-05
Applicant: KEYGENE N.V.
Inventor： Paul Bundock , Jeroen Stuurman
Abstract: The current disclosure relates to the field of plants, in particular to the fields of plant breeding and plant genetics. More particular, the disclosure concerns inventive methodology that may be useful in improving plant properties. In particular the invention may be useful in removing linkage drag. Also provided are plant and plant parts obtained with the method disclosed herein.
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公开(公告)号：US20250011800A1
公开(公告)日：2025-01-09
申请号：US18757549
申请日：2024-06-28
Applicant: Keygene N.V.
IPC: C12N15/82
Abstract: Method for targeted alteration of a duplex acceptor DNA sequence in a plant cell protoplast, comprising combining the duplex acceptor DNA sequence with a donor mutagenic nucleobase, wherein the duplex acceptor DNA sequence contains a first DNA sequence and a second DNA sequence which is the complement of the first DNA sequence and wherein the donor mutagenic nucleobase comprises at least one mismatch with respect to the duplex acceptor DNA sequence to be altered, preferably with respect to the first DNA sequence, wherein the method further comprises a step of introducing the donor mutagenic nucleobase into the cell protoplasts using polyethylene glycol (PEG) mediated transformation and the use of PEG protoplast transformation for enhancing the rate of targeted mutagenesis.
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公开(公告)号：US20240298589A1
公开(公告)日：2024-09-12
申请号：US18602867
申请日：2024-03-12
Applicant: Keygene N.V.
Inventor： Jeroen STUURMAN
Abstract: The present invention provides for a method for producing an inbred plant comprising a first and second trait of interest in the L1-shoot meristem layer for use in producing a periclinal chimera plant, the inbred plant thus obtained, the use of said inbred plant for producing said periclinal chimera plant, a method for producing a periclinal chimera plant using said inbred plant, a periclinal chimera plant thus obtained, the use of said periclinal chimera plant in producing plant product and the plant product thus obtained.
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