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    Mesenchymal stem cells as a vehicle for ion channel transfer in syncytial structures
    22.
    发明授权
    Mesenchymal stem cells as a vehicle for ion channel transfer in syncytial structures 有权
    间充质干细胞作为合胞体结构中离子通道转移的载体

    公开(公告)号：US07794702B2

    公开(公告)日：2010-09-14

    申请号：US10342506

    申请日：2003-01-15

    申请人： Michael R. Rosen ,  Richard B. Robinson ,  Ira S. Cohen ,  Peter Brink

    发明人： Michael R. Rosen ,  Richard B. Robinson ,  Ira S. Cohen ,  Peter Brink

    IPC分类号： A61K48/00

    CPC分类号： C12N5/0663 ,  A61K48/00 ,  C07K14/705

    摘要： This invention provides a composition for delivery of a gene to a syncytial structure comprising stem cells incorporated with the gene. This invention also provides a composition for ion channel transfer which comprises stem cells incorporated with a compound in an amount sufficient to create ion channels. This invention also provides for a method of expressing a functional gene product in a syncytial structure comprising administering a composition, comprising stem cells that have been incorporated with a gene, to the syncytial structure. This invention further provides a method of expressing a functional ion channel in a syncytial structure comprising administering a composition, comprising stem cells that have been incorporated with a compound in an amount sufficient to create ion channels, to the syncytial structure. This invention also provides a composition for delivery of small molecules comprising stem cells incorporated with the small molecules or genes encoding the small molecules.

    摘要翻译： 本发明提供了用于将基因递送至合成结构的组合物，其包括掺入该基因的干细胞。 本发明还提供了用于离子通道转移的组合物，其包含与足以产生离子通道的量的化合物结合的干细胞。 本发明还提供了一种在合胞体结构中表达功能基因产物的方法，其包括将包含基因的干细胞组合物施用于合胞体结构。 本发明进一步提供一种在合胞体结构中表达功能性离子通道的方法，其包括向合成结构施用包含与足以产生离子通道的化合物结合的干细胞的组合物。 本发明还提供了用于递送小分子的组合物，其包括与小分子结合的干细胞或编码小分子的基因。

    DEVICE AND METHOD FOR GROWING AND ANALYZING CELLS
    23.
    发明申请
    DEVICE AND METHOD FOR GROWING AND ANALYZING CELLS 失效
    用于生长和分析细胞的装置和方法

    公开(公告)号：US20100075362A1

    公开(公告)日：2010-03-25

    申请号：US12441117

    申请日：2007-09-10

    申请人： Richard B. Robinson ,  Lev Protas ,  Michael R. Rosen ,  Ira S. Cohen ,  Peter R. Brink

    发明人： Richard B. Robinson ,  Lev Protas ,  Michael R. Rosen ,  Ira S. Cohen ,  Peter R. Brink

    IPC分类号： C12Q1/02 ,  C12M1/00

    CPC分类号： C12M41/46 ,  C12M23/34 ,  C12M35/08

    摘要： A device and method for analyzing cells includes a housing with a chamber, a barrier supported by a frame disposed within the chamber, and a plate arranged at a bottom surface of the housing interior of the chamber. The plate is adapted to receive and sustain cells and the barrier separates the plate into at least two contiguous separate areas. In some embodiments, a thin rubber strip is arranged at the bottom edge of the barrier, which facilitates control of the area in which each cell type is grown, the size of the gap between the cells, and helps prevents over growth of the two cell types on to each other.

    摘要翻译： 用于分析细胞的装置和方法包括具有腔室的壳体，由设置在腔室内的框架支撑的屏障，以及布置在腔室的壳体内部的底表面处的板。 板适于接收和维持细胞，并且屏障将板分隔成至少两个连续的分离区域。 在一些实施例中，薄的橡胶条布置在阻挡层的底部边缘处，这有助于控制每个细胞类型生长的区域，细胞之间的间隙的大小，并且有助于防止两个细胞的过度生长 彼此之间的类型。

    COMPOSITIONS OF LATE PASSAGE MESENCHYMAL STEM CELLS (MSCS)
    25.
    发明申请
    COMPOSITIONS OF LATE PASSAGE MESENCHYMAL STEM CELLS (MSCS) 审中-公开
    最近一次通过的骨髓间充质干细胞（MSCS）

    公开(公告)号：US20100047216A1

    公开(公告)日：2010-02-25

    申请号：US12374027

    申请日：2007-07-20

    申请人： Glenn Gaudette ,  Irina A. Potapova ,  Peter R. Brink ,  Ira S. Cohen ,  Richard B. Robinson ,  Michael R. Rosen

    发明人： Glenn Gaudette ,  Irina A. Potapova ,  Peter R. Brink ,  Ira S. Cohen ,  Richard B. Robinson ,  Michael R. Rosen

    IPC分类号： A61K35/12 ,  C12N5/0775 ,  C12N5/10 ,  C12N5/00

    CPC分类号： C12N5/0663 ,  A61K35/12 ,  C12N2501/415 ,  C12N2510/00 ,  C12N2510/02

    摘要： The present invention provides methods and compositions relating to the use of late passage mesenchymal stem cells (MSCs) for treatment of cardiac disorders. Such late passage MSCs may be administered to the myocardium of a subject for induction of native cardiomyoctye proliferation and repair of cardiac tissue. Additionally, the late passage MSCs may be genetically engineered to express a gene encoding a physiologically active protein of interest and/or may be incorporated with small molecules for delivery to adjacent target cells through gap junctions. The late passage MSCs of the invention may be used to provide biological pacemaker activity and/or provide a bypass bridge in the heart of a subject afflicted with a cardiac rhythm disorder. The biological pacemaker activity and/or bypass bridge may be provided to the subject either alone or in tandem with an electronic pacemaker.

    摘要翻译： 本发明提供了与晚期传代间充质干细胞（MSC）用于治疗心脏病的方法和组合物。 这种晚期传代MSC可以施用于受试者的心肌，以诱导天然心肌细胞增殖和心脏组织的修复。 另外，晚期传代的MSC可被遗传工程化以表达编码目标生理活性蛋白质的基因和/或可与小分子结合，以通过间隙连接递送至相邻靶细胞。 本发明的晚期传代MSC可用于提供生物起搏器活动和/或在患有心律失常的受试者的心脏中提供旁路桥。 生物起搏器活动和/或旁通桥可以单独提供或与电子起搏器串联提供给受试者。

    Vectors encoding HCN channels and MiRP1
    26.
    发明授权
    Vectors encoding HCN channels and MiRP1 有权
    编码HCN通道和MiRP1的载体

    公开(公告)号：US06783979B2

    公开(公告)日：2004-08-31

    申请号：US09875392

    申请日：2001-06-06

    申请人： Michael R. Rosen ,  Richard B. Robinson ,  Ira S. Cohen ,  Han-Gang Yu

    发明人： Michael R. Rosen ,  Richard B. Robinson ,  Ira S. Cohen ,  Han-Gang Yu

    IPC分类号： C12N1500

    CPC分类号： G01N33/5061 ,  A61K38/1709 ,  A61K48/00 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/6872

    摘要： This invention provides for a chamber and system designed for use in assaying drug effects on heart rate. The chamber consists of a series of wells, each 3 mm by 3 mm in inner diameter. Cardiac myocytes disaggregated from neonatal animals are plated onto the bottom of each well and grown under standard tissue culture conditions. The chamber holds from 24-96 such wells. When drugs are to be assayed, the cells in each well are loaded with a calcium sensitive dye and the beating rate in each is monitored with a photodiode. Drug is added in graded concentrations to each well, and equilibrated and effects on rate are observed. This construct permits use of a cell based bioassay for the study of drugs or agents that may alter cardiac rate. This invention can be used in high throughput screening of drugs to evaluate/predict their effects on cardiac rate and rhythm. Further provided for by this invention is a vector which comprises a compound which encodes an ion channel.

    摘要翻译： 本发明提供了一种设计用于测定药物对心率的影响的室和系统。 该室由一系列的孔组成，内径为3 mm×3 mm。 将从新生儿动物分解的心肌细胞接种在每个孔的底部并在标准组织培养条件下生长。 该厅拥有24-96个这样的井。 当要测定药物时，每个孔中的细胞装载有钙敏感染料，并且用光电二极管监测每个孔中的搏动速率。 将药物以分级浓度加入每个孔中，并平衡并观察其对速率的影响。 该构建允许使用基于细胞的生物测定法来研究可能改变心率的药物或药剂。 本发明可用于药物的高通量筛选以评估/预测其对心率和节律的影响。 本发明进一步提供的是包含编码离子通道的化合物的载体。