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    COMPOSITIONS AND METHODS FOR BIASING THE POLARITY OF ORGANOIDS
    21.
    发明申请
    COMPOSITIONS AND METHODS FOR BIASING THE POLARITY OF ORGANOIDS 有权

    公开(公告)号：US20240376436A1

    公开(公告)日：2024-11-14

    申请号：US18569259

    申请日：2022-06-14

    Applicant: STEMCELL TECHNOLOGIES CANADA INC.

    Inventor： Salvatore SIMMINI ,  Juan HOU ,  Georgios STROULIOS ,  Tyler BROWN

    IPC: C12N5/071

    Abstract: The present disclosure relates to media compositions and/or supplements to be added into an organoid medium, and to methods for generating apical-out organoids. Apical-out organoids exhibit an apical surface thereof facing the external environment, and an inwardly disposed basolateral surface. Apical-out organoids may be formed in organoid media comprise a basal medium and one or more of an inhibitor of notch signaling and an inhibitor of signaling through a TGF. Apical-out organoids formed in accordance with the disclosed methods or in the disclosed media may be used to assay host responses to pathogens.

    HIGH-THROUGHPUT MICROFLUIDIC CHIP HAVING PARALLELIZED CONSTRICTIONS FOR PERTURBING CELL MEMBRANES
    22.
    发明申请
    HIGH-THROUGHPUT MICROFLUIDIC CHIP HAVING PARALLELIZED CONSTRICTIONS FOR PERTURBING CELL MEMBRANES 有权

    公开(公告)号：US20240376415A1

    公开(公告)日：2024-11-14

    申请号：US18665917

    申请日：2024-05-16

    Applicant: STEMCELL TECHNOLOGIES CANADA INC.

    Inventor： Maisam DADGAR ,  Jacquelyn L. Sikora HANSON ,  Armon R. SHAREI

    IPC: C12M3/06 ,  B01L3/00 ,  C12M1/26

    Abstract: A microfluidic chip for causing the delivery of a payload to a cell comprises a plurality of constrictions configured to allow a cell suspension to flow through one or more of the plurality of constrictions from a first fluid flow region to a second fluid flow region within the microfluidic chip, wherein a cross-sectional width of each of the plurality of constrictions is less than a diameter of cells in the cell suspension, such that membranes of the cells are perturbed when passing through the constrictions such that a payload is able to pass through the perturbed cell membranes, and wherein a quotient of a cross-sectional area over a cross-sectional perimeter of each of the plurality of constrictions is greater than greater than or equal to 0.5 μm.

    METHODS AND ASSAYS WITH POPULATIONS OF CELLS
    24.
    发明申请
    METHODS AND ASSAYS WITH POPULATIONS OF CELLS 有权

    公开(公告)号：US20230082631A1

    公开(公告)日：2023-03-16

    申请号：US17800808

    申请日：2021-02-19

    Applicant: StemCell Technologies Canada Inc.

    Inventor： Trevor ROGERS

    IPC: C12N5/00 ,  G01N15/14 ,  G01N33/543

    Abstract: This disclosure relates to methods for enriching a first population of cells positive for a target moiety and/or a second population of cells positive for the target moiety from a sample, wherein a level of the target moiety among the first population of cells is relatively lower than the level of the target moiety among the second population of cells. The methods of this disclosure may also be adapted to assays for determining distinct populations of cells positive for a target moiety in a sample, and to assays for optimizing enrichment conditions. Last, this disclosure relates to kits of components that may be used to carry out the methods and assays.

    Method for separating cells using immunorosettes and magnetic particles
    25.
    发明授权
    Method for separating cells using immunorosettes and magnetic particles 有权

    公开(公告)号：US11325984B2

    公开(公告)日：2022-05-10

    申请号：US16108707

    申请日：2018-08-22

    Applicant: StemCell Technologies Canada Inc.

    Inventor： Andy Isamu Kokaji

    IPC: G01N33/569 ,  C07K16/42 ,  C07K16/28 ,  G01N1/40

    Abstract: The present invention relates to methods for separating target cells from non-target cells using immunorosettes and magnetic particles. The method involves contacting a sample containing target cells and secondary targets such as erythrocytes with an antibody composition which allows immunorosettes of the target cells and the secondary targets to form. The sample is subsequently contacted with a second antibody composition which allows the binding of magnetic particles to the formed immunorosettes and free secondary targets. The immunorosettes and secondary targets labeled with magnetic particles are separated from non-target cells using a magnetic field. The antibody composition optionally contains bifunctional antibodies or tetrameric antibody complexes.

    SYSTEM FOR DELIVERY OF A PAYLOAD INTO A CELL
    27.
    发明申请
    SYSTEM FOR DELIVERY OF A PAYLOAD INTO A CELL 有权

    公开(公告)号：US20250115854A1

    公开(公告)日：2025-04-10

    申请号：US18983907

    申请日：2024-12-17

    Applicant: STEMCELL TECHNOLOGIES CANADA INC.

    Inventor： Maisam DADGAR ,  Tarek ABDELJAWAD ,  Howard BERNSTEIN

    IPC: C12M1/42 ,  B01L3/00 ,  C12M3/06 ,  G01N1/31

    Abstract: A system for delivering a payload to a cell that includes: a platform supporting an input container, an output container, and a receiver for receiving all or part of a disposable assembly, the disposable assembly including a preparation vessel and a constriction cartridge. The preparation vessel holds a cell suspension as it is prepared for passage through one or more cell-deforming constrictions, and the constriction cartridge houses a component that includes the one or more cell deforming constrictions. Passage through the cell-deforming constrictions causes perturbations in cell membranes to allow entry of a payload into the cells. The system includes one or more processors configured to receive input from a user and to automatically control pressure, temperature, agitation, and/or flow of the cell suspension as it passes through the input container, through the preparation vessel, through the constriction cartridge, and to the output container.

    COMPOSITIONS AND METHODS FOR EXPANDING KERATINOCYTES
    28.
    发明申请
    COMPOSITIONS AND METHODS FOR EXPANDING KERATINOCYTES 有权

    公开(公告)号：US20250043250A1

    公开(公告)日：2025-02-06

    申请号：US18718866

    申请日：2022-12-15

    Applicant: STEMCELL Technologies Canada Inc.

    Inventor： Yaoyao Chen ,  Wing Chang ,  Victoria Stephenson

    IPC: C12N5/071

    Abstract: This disclosure relates to media compositions and/or supplements to be added into a medium, and to methods for culturing and/or expanding keratinocytes or keratinocyte-like cells. In another aspect, this disclosure relates to media composition and/or supplements to be added into a medium, and to methods of providing an enriched or selected for population of keratinocytes or keratinocyte-like cells. Keratinocytes or keratinocyte-like cells may be derived from pluripotent stem cells, and more particularly from a differentiated population of pluripotent stem cells.

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