公开(公告)号：US20080255006A1

公开(公告)日：2008-10-16

申请号：US11938187

申请日：2007-11-09

Applicant: Shan X. Wang ,  Robert L. White ,  Chris D. Webb ,  Guanxiong Li

Inventor： Shan X. Wang ,  Robert L. White ,  Chris D. Webb ,  Guanxiong Li

IPC: C40B60/12

CPC classification number: H01F10/3272 ,  B01J2219/00378 ,  B01J2219/00497 ,  B01J2219/00563 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00653 ,  B01J2219/00655 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00734 ,  B01L3/5027 ,  B82Y5/00 ,  B82Y15/00 ,  B82Y25/00 ,  B82Y30/00 ,  C12Q1/6834 ,  C40B40/06 ,  C40B40/10 ,  C40B40/14 ,  C40B60/14 ,  C40B70/00 ,  G01N33/54326 ,  G01N33/5434 ,  G01N33/553 ,  G01N2446/10 ,  H01F1/0054 ,  H01F10/3254 ,  Y10T428/2991 ,  C12Q2563/143

Abstract: Magnetic nanoparticles and methods for their use in detecting biological molecules are disclosed. The magnetic nanoparticles can be attached to nucleic acid molecules, which are then captured by a complementary sequence attached to a detector, such as a spin valve detector or a magnetic tunnel junction detector. The detection of the bound magnetic nanoparticle can be achieved with high specificity and sensitivity.

Abstract translation: 公开了磁性纳米颗粒及其用于检测生物分子的方法。 磁性纳米颗粒可以连接到核酸分子，然后通过与检测器（例如自旋阀检测器或磁性隧道结检测器）连接的互补序列捕获。 可以以高特异性和灵敏度实现结合的磁性纳米颗粒的检测。

公开(公告)号：US20080206891A1

公开(公告)日：2008-08-28

申请号：US11894046

申请日：2007-08-16

Applicant: Shan X. Wang ,  Robert John Wilson ,  Wei Hu

Inventor： Shan X. Wang ,  Robert John Wilson ,  Wei Hu

IPC: G01N33/553

CPC classification number: G01N33/5434 ,  B03C1/01 ,  B82Y25/00 ,  H01F1/0054 ,  H01F10/3272

Abstract: The present invention provides a synthetic antiferromagnetic (SAF) nanoparticle. The SAF nanoparticle includes at least two ferromagnetic layers and at least one non-magnetic spacer layer. The spacer layer is situated in between planar surfaces of the ferromagnetic layers. The saturation field of the SAF nanoparticle is tunable by the geometry and composition of the nanoparticle. Preferably, the saturation field can be tuned to be between about 100 Oe and about 10,000 Oe. Also preferably, the SAF nanoparticle has a magnetic moment of at least 800 emu/cm3. In a preferred embodiment, the SAF nanoparticle has at least one of a biomolecule, a recognition moiety, or a molecular coating attached to its surface. The SAF nanoparticle may also have a dye attached to its surface.

Abstract translation: 本发明提供一种合成反铁磁（SAF）纳米颗粒。 SAF纳米颗粒包括至少两个铁磁层和至少一个非磁性间隔层。 间隔层位于铁磁层的平坦表面之间。 SAF纳米颗粒的饱和场可以通过纳米颗粒的几何形状和组成来调节。 优选地，饱和场可以调节在约100Oe和约10,000Oe之间。 还优选地，SAF纳米颗粒具有至少800emu / cm 3的磁矩。 在优选的实施方案中，SAF纳米颗粒具有附着于其表面的生物分子，识别部分或分子涂层中的至少一种。 SAF纳米颗粒也可以具有附着在其表面上的染料。

公开(公告)号：US09863939B2

公开(公告)日：2018-01-09

申请号：US12234506

申请日：2008-09-19

Applicant: Shan X. Wang ,  Sebastian J. Osterfeld ,  Heng Yu ,  Nader Pourmand ,  Robert L. White

Inventor： Shan X. Wang ,  Sebastian J. Osterfeld ,  Heng Yu ,  Nader Pourmand ,  Robert L. White

IPC: G01N33/00 ,  G01N33/543 ,  G01N27/00

CPC classification number: G01N33/54326 ,  Y10T436/143333

Abstract: Methods for analyte detection with magnetic sensors are provided. Aspects of the methods include producing a magnetic sensor device having a magnetically labeled analyte from a sample, such as a serum sample, bound to a surface of a magnetic sensor thereof; and obtaining a signal, e.g., a real-time signal, from the magnetic sensor to determine whether the analyte is present in the sample. Also provided are devices, systems and kits that find use in practicing the methods of the invention. The methods, devices, systems and kits of the invention find use in a variety of different applications, including detection of biomarkers, such as disease markers.

公开(公告)号：US08722017B2

公开(公告)日：2014-05-13

申请号：US12460007

申请日：2009-07-10

Applicant: Aihua Fu ,  Shan X. Wang ,  Sanjiv Sam Gambhir

Inventor： Aihua Fu ,  Shan X. Wang ,  Sanjiv Sam Gambhir

IPC: A61B5/055

CPC classification number: A61K49/1848 ,  A61K9/5094 ,  A61K49/0002 ,  A61K49/0032 ,  A61K49/0045 ,  A61K49/005 ,  A61K49/0093 ,  A61K49/1833 ,  A61K49/1866 ,  A61K51/1244 ,  B82Y5/00 ,  B82Y15/00 ,  G01N33/5434 ,  G01N33/582 ,  G01N33/587

Abstract: Embodiments of the present disclosure provide for nanoprobes, methods of imaging, methods of imaging a target, methods of making nanoprobes, and the like.

公开(公告)号：US20110250464A1

公开(公告)日：2011-10-13

申请号：US13066248

申请日：2011-04-08

Applicant: Robert J. Wilson ,  Jung-Sub Wi ,  Shan X. Wang ,  Edward S. Barnard ,  Mark L. Brongersma ,  Mary Tang

Inventor： Robert J. Wilson ,  Jung-Sub Wi ,  Shan X. Wang ,  Edward S. Barnard ,  Mark L. Brongersma ,  Mary Tang

IPC: B22F1/00

CPC classification number: B22F1/0018 ,  B22F7/08 ,  B22F2001/0037 ,  B82Y30/00

Abstract: A Raman-active nanoparticle is provided that includes a dish-shape plasmonically active metal base, and a plasmonically active metal pillar disposed on the plasmonically active metal base, where the plasmonically active metal pillar is disposed within the dish-shape plasmonically active metal base and normal to a bottom of the dish-shape plasmonically active metal base, where a circular gap is disposed between the dish-shape plasmonically active metal pillar and inner walls of the dish-shape plasmonically active metal base. In one embodiment a Raman-active nanoparticle is provided that includes a dish-shape base having a dielectric material, an electrically conductive layer disposed on the inner surface of the dish-shape base, and an electrically conductive pillar disposed on the conductive layer, and within the dish-shape and perpendicular to a bottom of the dish-shape base, where a circular gap is disposed between the conductive pillar and inner walls of the dish-shape base.

Abstract translation: 提供了一种拉曼活性纳米颗粒，其包括盘状等离子体活性金属碱和设置在等离子体活性金属基体上的等离子体活性金属柱，其中等离子体活性金属柱设置在盘状等离子体活性金属基质内， 垂直于盘形等离子体活性金属基底的底部，其中盘形等离子体活性金属柱和盘形等离子体活性金属基底的内壁之间设置有圆形间隙。 在一个实施方案中，提供了拉曼活性纳米颗粒，其包括具有介电材料的盘形基底，设置在盘形基底的内表面上的导电层和设置在导电层上的导电柱，以及 在碟形基底中的垂直于盘形基底的底部，其中圆形间隙设置在盘形基底的导电柱和内壁之间。

公开(公告)号：US20110223612A1

公开(公告)日：2011-09-15

申请号：US13046368

申请日：2011-03-11

Applicant: Shan X. Wang ,  Richard S. Gaster ,  Liang Xu ,  Shu-Jen Han ,  Robert Wilson

Inventor： Shan X. Wang ,  Richard S. Gaster ,  Liang Xu ,  Shu-Jen Han ,  Robert Wilson

IPC: G01N33/557 ,  G01N33/566 ,  G01N33/68 ,  G01N27/72

CPC classification number: G01N27/745 ,  G01N33/557 ,  Y10T436/143333

Abstract: Methods for quantitatively determining a binding kinetic parameter of a molecular binding interaction are provided. Aspects of embodiments of the methods include: producing a magnetic sensor device including a magnetic sensor in contact with an assay mixture including a magnetically labeled molecule to produce a detectable molecular binding interaction; obtaining a real-time signal from the magnetic sensor; and quantitatively determining a binding kinetics parameter of the molecular binding interaction from the real-time signal. Also provided are systems and kits configured for use in the methods.

Abstract translation: 提供了定量测定分子结合相互作用的结合动力学参数的方法。 所述方法的实施方案的方面包括：产生包括与包含磁性标记分子的测定混合物接触以产生可检测分子结合相互作用的磁传感器的磁传感器装置; 从磁传感器获取实时信号; 并从实时信号中定量测定分子结合相互作用的结合动力学参数。 还提供了配置用于该方法的系统和套件。

公开(公告)号：US07989396B2

公开(公告)日：2011-08-02

申请号：US11949442

申请日：2007-12-03

Applicant: Heng Yu ,  Nader Pourmand ,  Shan X. Wang

Inventor： Heng Yu ,  Nader Pourmand ,  Shan X. Wang

IPC: C40B50/18 ,  C40B60/02

CPC classification number: C40B60/12 ,  C40B40/08 ,  C40B40/10 ,  C40B50/18 ,  G01N33/54353

Abstract: A highly specific and versatile surface chemistry for immobilization of amine-terminated probes is disclosed. A bi-layered polymer thin film serves as the platform for coupling the probes, which are preferably oligonucleotides. The process involves sequentially coating a substrate with polyamine and polyacid anhydride. Hydrolyzed polyacid anhydride groups may be converted to non-hydrolyzed groups at about 100° C. prior to probe attachment. The process of coating the substrate requires no harsh chemical pretreatment of substrates such as RCA or Piranha cleaning. In addition, simple thermal activation of the anhydride groups has a low requirement for storage, leading to a long shelf life of modified surfaces. The disclosed surface chemistry is especially compatible with microfabrication processes, and its effective application to magnetic biosensors is demonstrated.

Abstract translation: 公开了用于固定胺封端的探针的高度特异性和多功能的表面化学。 双层聚合物薄膜用作耦合探针的平台，优选为寡核苷酸。 该方法包括依次用多胺和多酸酐涂覆底物。 在探针附着之前，水解的多酸酐基团可以在约100℃下转化为非水解基团。 涂覆基材的过程不需要对基材进行苛刻的化学预处理，例如RCA或Piranha清洗。 此外，酸酐基团的简单热活化对储存的要求较低，导致改性表面的保质期长。 所公开的表面化学特性与微细加工工艺特别兼容，并且证明了其在磁性生物传感器中的有效应用。

公开(公告)号：US20110059468A1

公开(公告)日：2011-03-10

申请号：US12807625

申请日：2010-09-09

Applicant: Christopher M. Earhart ,  Shan X. Wang ,  Robert J. Wilson

Inventor： Christopher M. Earhart ,  Shan X. Wang ,  Robert J. Wilson

IPC: G01N33/53 ,  C12M1/00 ,  C12N13/00 ,  C12Q1/06

CPC classification number: C12N13/00 ,  G01N33/54333 ,  G01N33/56966 ,  G01N33/57488

Abstract: A magnetic sifter is adapted for manipulation of biological cells by providing a greater pore density at the edge of the sifter than at the center. Application of an external magnetic field to the sifter causes high magnetic fields and field gradients at the sifter pores. These conditions are suitable for capturing magnetically tagged or labeled cells at the sifter pores. Altering the external magnetic field can provide controlled capture and/or release of magnetically labeled cells from the sifter pores. The purpose of having a greater pore density at the periphery of the sifter than at the center is to provide improved flow rate uniformity through the sifter. Such flow rate uniformity is advantageous for cell quantification.

Abstract translation: 磁选机适用于通过在筛子的边缘处提供比在中心处更大的孔密度来操纵生物细胞。 在筛子上施加外部磁场会在筛分孔产生高磁场和场梯度。 这些条件适用于在筛子孔处捕获磁标记或标记的细胞。 改变外部磁场可以提供来自筛子孔的磁性标记细胞的受控捕获和/或释放。 在筛子周边具有比中心更大的孔密度的目的是通过筛子提供改善的流速均匀性。 这样的流速均匀性对于细胞定量是有利的。

公开(公告)号：US20090104707A1

公开(公告)日：2009-04-23

申请号：US12234506

申请日：2008-09-19

Applicant: Shan X. Wang ,  Sebastian J. Osterfeld ,  Heng Yu ,  Nader Pourmand ,  Robert L. White

Inventor： Shan X. Wang ,  Sebastian J. Osterfeld ,  Heng Yu ,  Nader Pourmand ,  Robert L. White

IPC: G01N33/00 ,  G01N27/00 ,  B01J19/00

CPC classification number: G01N33/54326 ,  Y10T436/143333

Abstract: Methods for analyte detection with magnetic sensors are provided. Aspects of the methods include producing a magnetic sensor device having a magnetically labeled analyte from a sample, such as a serum sample, bound to a surface of a magnetic sensor thereof; and obtaining a signal, e.g., a real-time signal, from the magnetic sensor to determine whether the analyte is present in the sample. Also provided are devices, systems and kits that find use in practicing the methods of the invention. The methods, devices, systems and kits of the invention find use in a variety of different applications, including detection of biomarkers, such as disease markers.

Abstract translation: 提供了使用磁传感器进行分析物检测的方法。 所述方法的方面包括制备具有磁性标记的分析物的磁性传感器装置，所述磁性传感器装置与来自其磁性传感器的表面的样品（例如血清样品）结合; 以及从磁传感器获得信号，例如实时信号，以确定分析物是否存在于样品中。 还提供了用于实践本发明的方法的装置，系统和试剂盒。 本发明的方法，装置，系统和试剂盒可用于各种不同的应用，包括检测生物标志物，例如疾病标记物。

公开(公告)号：US07501253B2

公开(公告)日：2009-03-10

申请号：US11789559

申请日：2007-04-24

Applicant: Nader Pourmand ,  Ronald W. Davis ,  Shan X. Wang

Inventor： Nader Pourmand ,  Ronald W. Davis ,  Shan X. Wang

IPC: C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/6837 ,  C12Q2565/519 ,  C12Q2537/1373 ,  C12Q2525/204

Abstract: A method of determining the length of a polynucleotide target is provided. With this method, a target is first hybridized to an array of first probes having different, determined lengths, resulting in the formation of duplexes between the polynucleotide target and the first probes. These duplexes have a single stranded section of target if the target is longer than the first probe it is in a duplex with, and a single stranded section of probe if the target is shorter than the first probe it is in a duplex with. Next, a series of probes is hybridized to the duplexes, breaking apart duplexes in which the target and probe have unequal lengths through the process of branch migration. Thus, the target only remains bound in the duplex if the target and probe are of equal lengths. The length of the polynucleotide target can thereby be determined.

Abstract translation: 提供了确定多核苷酸靶标长度的方法。 利用该方法，首先将靶与具有不同的确定长度的第一探针阵列杂交，导致在多核苷酸靶和第一探针之间形成双链体。 如果目标比第一探针长两倍以上，那么这些双链体具有目标的单链段，如果目标短于其双链体中的第一探针，则其具有探针的单链段。 接下来，一系列探针与双链体杂交，分离双链体，其中靶和探针通过分支迁移过程具有不等长度。 因此，如果目标和探针具有相等的长度，则目标仅保持在双工中。 因此可以确定多核苷酸靶的长度。