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34 条记录 (耗时 0.026 秒)

    Method for cloning and expression of AsiSI restriction endonuclease and AsiSI methylase in E. coli
    21.
    发明授权
    Method for cloning and expression of AsiSI restriction endonuclease and AsiSI methylase in E. coli 有权
    在大肠杆菌中克隆和表达AsiSI限制性内切核酸酶和AsiSI甲基化酶的方法

    公开(公告)号：US06514737B1

    公开(公告)日：2003-02-04

    申请号：US09933313

    申请日：2001-08-20

    申请人： Zhenyu Zhu ,  Shuang-yong Xu

    发明人： Zhenyu Zhu ,  Shuang-yong Xu

    IPC分类号： C12N912

    CPC分类号： C12N9/1007 ,  C12N9/22

    摘要： The present invention relates to recombinant DNA which encodes the AsiSI restriction endonuclease as well as AsiSI methylase, expression of AsiSI restriction endonuclease and AsiSI methylase in E. coli cells containing the recombinant DNA.

    摘要翻译： 本发明涉及编码AsiSI限制性内切核酸酶以及AsiSI甲基化酶，AsiSI限制性内切核酸酶和AsiSI甲基化酶在含有重组DNA的大肠杆菌细胞中的表达的重组DNA。

    Method for cloning and expression of Bpml restriction endonuclease in E. coli
    22.
    发明授权
    Method for cloning and expression of Bpml restriction endonuclease in E. coli 有权
    在大肠杆菌中克隆和表达Bpml限制性内切核酸酶的方法

    公开(公告)号：US06413758B1

    公开(公告)日：2002-07-02

    申请号：US09693146

    申请日：2000-10-20

    申请人： Shuang-yong Xu ,  Jian-ping Xiao ,  Zhenyu Zhu

    发明人： Shuang-yong Xu ,  Jian-ping Xiao ,  Zhenyu Zhu

    IPC分类号： C12N922

    CPC分类号： C12N9/22 ,  C07K2319/00 ,  C12N9/1007

    摘要： The present invention relates to recombinant DNA which encodes the BpmI restriction endonuclease as well as BpmI methyltransferase, expression of BpmI restriction endonuclease from E. coli cells containing the recombinant DNA. BpmI endonuclease is a fusion of two distinct elements with a possible structural domains of restriction-methylation-specificity (R-M-S). This domain organization is analogous to the type I restriction-modification system with three distinct subunits, restriction, methylation, and specificity (R, M, and S). Because BpmI is quite distinct to other type IIs restriction enzymes, it is proposed that BpmI belongs to a subgroup of type II restriction enzymes called type IIf (f stands for fusion of restriction-modification-specificity domains). The Type IIf group of restriction enzyme includes Eco57I, BpmI, GsuI, BseRI and some other restriction enzymes that cut downstream sequences at long distance, 10-20 bp downstream of recognition sequence, such as MmeI (N20/N18)).

    摘要翻译： 本发明涉及编码BpmI限制性内切核酸酶以及BpmI甲基转移酶的重组DNA，含有重组DNA的大肠杆菌细胞中BpmI限制性内切核酸酶的表达。 BpmI内切核酸酶是具有限制性甲基化特异性（R-M-S）的可能结构域的两个不同元件的融合物。 这种结构域组织类似于具有三个不同亚基，限制性，甲基化和特异性（R，M和S）的I型限制性修饰系统。 由于BpmI与其他II型限制性内切酶相当不同，所以提出BpmI属于称为IIf型的II型限制酶亚型（f代表限制性修饰特异性结构域的融合）。 IIf类限制酶包括Eco57I，BpmI，GsuI，BseRI和一些其他限制酶，其在识别序列下游10-20bp处长距离切割下游序列，例如MmeI（N20 / N18））。

    Modular Computer Application Development and Usage
    24.
    发明申请
    Modular Computer Application Development and Usage 审中-公开
    模块化计算机应用开发与使用

    公开(公告)号：US20170068523A1

    公开(公告)日：2017-03-09

    申请号：US14846527

    申请日：2015-09-04

    申请人： Zhenyu Zhu

    发明人： Zhenyu Zhu

    IPC分类号： G06F9/445 ,  H04L29/08

    CPC分类号： G06F8/60 ,  G06F8/34 ,  G06F8/36 ,  H04L67/1097

    摘要： This is a process, with supporting apparatus, to facilitate modular software application and knowledgebase development and usage in a searchable, cumulative and automated manner.Developer and users deploy and access the application/knowledgebase with integrated process through the application call manager. A standardized data container is used to store various types of data in the entire process. Depends of user action, the process can automatically record or use parametric information of the target applications to manual or batch call applications or load knowledgebase of the applications.The standardized data container also serves a data and application call knowledgebase template and facilitates knowledge transfer between users and developers.

    摘要翻译： 标准化数据容器还提供数据和应用程序调用知识库模板，并促进用户和开发人员之间的知识传递。

    USB DEVICE CONTROL USING ENDPOINT TYPE DETECTION DURING ENUMERATION
    26.
    发明申请
    USB DEVICE CONTROL USING ENDPOINT TYPE DETECTION DURING ENUMERATION 有权
    USB设备控制使用终端类型检测

    公开(公告)号：US20130346660A1

    公开(公告)日：2013-12-26

    申请号：US13532730

    申请日：2012-06-25

    申请人： Piotr Kwidzinski ,  Zhenyu Zhu

    发明人： Piotr Kwidzinski ,  Zhenyu Zhu

    IPC分类号： G06F13/14

    CPC分类号： G06F21/85 ,  G06F13/126 ,  G06F13/426

    摘要： Described herein are embodiments of USB device control using endpoint type detection during enumeration. An apparatus configured for USB device control using endpoint type detection during enumeration may include a host controller configured to selectively disable enumeration of a USB device based at least in part on an endpoint type of the USB device. The apparatus may include a management engine configured to store in the host controller a USB lock policy defining endpoint types disallowed to be enumerated by the apparatus. Other embodiments may be described and/or claimed.

    摘要翻译： 这里描述的是在枚举期间使用端点类型检测的USB设备控制的实施例。 配置用于在枚举期间使用端点类型检测的USB设备控制的设备可以包括被配置为至少部分地基于USB设备的端点类型来选择性地禁用USB设备的枚举的主机控制器。 该设备可以包括管理引擎，其被配置为在主机控制器中存储定义不允许被该设备枚举的端点类型的USB锁定策略。 可以描述和/或要求保护其他实施例。

    NICKING ENDONUCLEASE METHODS AND COMPOSITIONS
    29.
    发明申请
    NICKING ENDONUCLEASE METHODS AND COMPOSITIONS 有权
    使用内切酶方法和组合物

    公开(公告)号：US20110076720A1

    公开(公告)日：2011-03-31

    申请号：US12906515

    申请日：2010-10-18

    申请人： Shuang-Yong Xu ,  Zhenyu Zhu ,  Timothy Meixsell

    发明人： Shuang-Yong Xu ,  Zhenyu Zhu ,  Timothy Meixsell

    IPC分类号： C12P21/06 ,  C12N9/22

    CPC分类号： C12N9/22

    摘要： A nicking endonuclease is described which has an amino acid sequence with at least 70% identity to SEQ ID NO:6 and comprising a mutation at least one of an arginine or gutamic acid corresponding to position 507 and position 546 respectively in SEQ ID NO:6.

    摘要翻译： 描述了一种切口内切核酸酶，其具有与SEQ ID NO：6具有至少70％同一性的氨基酸序列，并且包含分别对应于SEQ ID NO：6中的位置507和位置546的精氨酸或谷氨酸中的至少一种的突变 。