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    Δ17 desaturase and its use in making polyunsaturated fatty acids
    21.
    发明授权
    Δ17 desaturase and its use in making polyunsaturated fatty acids 有权
    去饱和酶及其在制备多不饱和脂肪酸中的应用

    公开(公告)号：US08241884B2

    公开(公告)日：2012-08-14

    申请号：US12469208

    申请日：2009-05-20

    Applicant: Narendra S. Yadav ,  Zhixiong Xue ,  Quinn Qun Zhu

    Inventor： Narendra S. Yadav ,  Zhixiong Xue ,  Quinn Qun Zhu

    IPC: C12N1/16

    CPC classification number: C12P7/6427 ,  C12N9/0083 ,  C12P7/6472

    Abstract: The present invention relates to Δ17 desaturases, which have the ability to convert ω-6 fatty acids into their ω-3 counterparts (i.e., conversion of arachidonic acid [20:4, ARA] to eicosapentaenoic acid [20:5, EPA]). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ17 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ17 desaturases in oleaginous yeast are disclosed.

    Abstract translation: 本发明涉及具有将ω-6脂肪酸转化成其ω-3对应物的能力（即将花生四烯酸[20:4，ARA]转化为二十碳五烯酸[20:5，EPA ]）。 公开了分离的核酸片段和重组构建体，其包含编码＆amp; 17去饱和酶的这种片段以及使用这些在油性酵母中的这些＆Dgr.17去饱和酶制备长链多不饱和脂肪酸（PUFA）的方法。

    AMMONIUM TRANSPORTER PROMOTERS FOR GENE EXPRESSION IN OLEAGINOUS YEAST
    25.
    发明申请
    AMMONIUM TRANSPORTER PROMOTERS FOR GENE EXPRESSION IN OLEAGINOUS YEAST 有权
    用于亚历山大·基因表达的氨基转运酶促进剂

    公开(公告)号：US20100068789A1

    公开(公告)日：2010-03-18

    申请号：US12618070

    申请日：2009-11-13

    Applicant: ZHIXIONG XUE ,  QUINN QUN ZHU

    Inventor： ZHIXIONG XUE ,  QUINN QUN ZHU

    IPC: C12N1/19 ,  C12N15/74

    CPC classification number: C12N15/81

    Abstract: The promoter region associated with the Yarrowia lipolytica ammonium transporter (yat1) gene has been found to be particularly effective for the expression of heterologous genes in oleaginous yeast. The promoter regions of the instant invention have been shown to be advantageously inducible under oleaginous conditions (i.e., nitrogen limitation) and are useful to drive expression of genes involved in the production of ω-3 and ω-6 fatty acids.

    Abstract translation: 已经发现与解脂耶氏酵母转氨酶（yat1）基因相关的启动子区域在含油酵母中表达异源基因特别有效。 已显示本发明的启动子区域在油性条件（即氮限制）下是有利的可诱导的，并且可用于驱动参与ω-3和ω-6脂肪酸生产的基因的表达。

    Method of production of para-hydroxycinnamic acid using a thermostable TAL enzyme
    26.
    发明授权
    Method of production of para-hydroxycinnamic acid using a thermostable TAL enzyme 有权
    使用耐热TAL酶制备对羟基肉桂酸的方法

    公开(公告)号：US07572612B2

    公开(公告)日：2009-08-11

    申请号：US11485558

    申请日：2006-07-12

    Applicant: Lixuan Lisa Huang ,  Zhixiong Xue ,  Michael P. McCluskey

    Inventor： Lixuan Lisa Huang ,  Zhixiong Xue ,  Michael P. McCluskey

    IPC: C12P7/42 ,  C12N1/20

    CPC classification number: C12N9/88 ,  C12P7/42

    Abstract: A thermostable TAL enzyme was identified from the fungus Phanerochaete chrysosporium, which has high activity at temperatures of 40° C. to about 60° C. The enzyme was produced in engineered cells and used for production of para-hydroxycinnamic acid (pHCA) from tyrosine. When the pHCA production reaction was run at high temperature, the enzyme was more active and pHCA was produced more rapidly, making the reaction more efficient.

    Abstract translation: 从真菌Phanerochaete chrysosporium鉴定出热稳定的TAL酶，其在40℃至约60℃的温度下具有高活性。该酶在工程细胞中产生，用于从酪氨酸生产对羟基肉桂酸（pHCA） 。 当pHCA生产反应在高温下运行时，酶活性更高，pHCA产生更快，使反应更有效。

    Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
    28.
    发明授权
    Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms 有权
    高山被孢霉二酰基甘油酰基转移酶用于改变多不饱和脂肪酸和含油生物中的油含量

    公开(公告)号：US07198937B2

    公开(公告)日：2007-04-03

    申请号：US11024545

    申请日：2004-12-29

    Applicant: Zhixiong Xue ,  Narendra S. Yadav ,  Daniel Joseph Macool

    Inventor： Zhixiong Xue ,  Narendra S. Yadav ,  Daniel Joseph Macool

    IPC: C12N1/16 ,  C12N1/13 ,  C12N1/19 ,  C12N1/18 ,  C12N9/10 ,  C12P21/06 ,  C07H21/04

    CPC classification number: C12P7/6472 ,  C12N9/1029 ,  C12P7/6463

    Abstract: An acyltransferase is provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous organisms. Specifically, the gene encoding diacylglycerol acyltransferase (DGAT2) has been isolated from Mortierella alpina. This gene encodes an enzyme that participates in the terminal step in oil biosynthesis in fungi and yeast and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids produced in oils of oleaginous organisms. Most desirably, the substrate specificity of the instant DGAT2 will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.

    Abstract translation: 提供了一种酰基转移酶，适用于制造含油生物体中富含ω-脂肪酸的微生物油。 具体地，从高山被孢霉中分离出编码二酰基甘油酰基转移酶（DGAT2）的基因。 该基因编码参与真菌和酵母中油生物合成的最终步骤的酶，并且预期在改变油性生物油中产生的长链多不饱和脂肪酸的量方面起关键作用。 最理想的是，即时DGAT2的底物特异性将特别适用于在含油酵母例如解脂耶氏酵母中积累长链等于或大于C 20的长链PUFA。

    Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
    29.
    发明授权
    Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms 失效
    高山被孢霉甘油-3-磷酸O-酰基转移酶用于改变多不饱和脂肪酸和含油生物中的油含量

    公开(公告)号：US07192762B2

    公开(公告)日：2007-03-20

    申请号：US11254173

    申请日：2005-10-19

    Applicant: Daniel Joseph Macool ,  Zhixiong Xue

    Inventor： Daniel Joseph Macool ,  Zhixiong Xue

    IPC: C12N1/18 ,  C12N15/70 ,  C12N9/10 ,  C12N1/13 ,  C12Q1/68 ,  C12P21/06 ,  C07H21/04 ,  C12N1/19 ,  C12N1/16

    CPC classification number: C12P7/6472 ,  C12N9/1029 ,  C12P7/6463

    Abstract: Glycerol-3-phosphate o-acyltransferase (GPAT) participates in the first step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids (PUFAs) produced in oils of oleaginous organisms. The present application provides a nucleic acid fragment isolated from Mortierella alpina encoding a GPAT that is suitable for use in the manufacture of oils enriched in omega fatty acids in oleaginous organisms. Most desirably, the substrate specificity of the instant GPAT will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.

    Abstract translation: 甘油-3-磷酸O-酰基转移酶（GPAT）参与石油生物合成的第一步，预计在改变含油生物油中产生的长链多不饱和脂肪酸（PUFA）的量方面发挥关键作用。 本申请提供了从编码GPAT的高山被孢霉分离的核酸片段，该GPAT适用于制造含油生物体中富含ω-脂肪酸的油。 最理想的是，本发明的GPAT的底物特异性将特别适用于在含油酵母例如解脂耶氏酵母（Yarrowia lipolytica）中积累具有等于或大于C 20的链长度的长链PUFA。

    Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
    30.
    发明授权
    Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms 有权
    高山被孢霉溶血磷脂酸酰基转移酶同源物，用于改变多不饱和脂肪酸和含油生物中的油含量

    公开(公告)号：US07189559B2

    公开(公告)日：2007-03-13

    申请号：US11251466

    申请日：2005-10-14

    Applicant: Howard Glenn Damude ,  Zhixiong Xue

    Inventor： Howard Glenn Damude ,  Zhixiong Xue

    IPC: C12N1/16 ,  C12N9/10 ,  C12N15/70 ,  C12N15/04 ,  C12P21/06 ,  C07H21/04

    CPC classification number: C12P7/6472 ,  C12N9/1029 ,  C12P7/6463

    Abstract: Lysophosphatidic acid acyltransferase (LPAAT) participates in the second step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids produced in oils of oleaginous organisms. The present application provides a nucleic acid fragment (identified as “LPAAT2”) isolated from Mortierella alpina encoding a LPAAT homolog that is suitable for use in the manufacture of oils enriched in omega fatty acids in oleaginous organisms. Most desirably, the substrate specificity of the instant LPAAT2 will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.

    Abstract translation: 溶血磷脂酰基转移酶（LPAAT）参与石油生物合成的第二步，预计在改变含油生物油中产生的长链多不饱和脂肪酸的量方面发挥关键作用。 本申请提供了从编码LPAAT同源物的高山被孢霉分离的核酸片段（鉴定为“LPAAT2”），其适用于制造含油生物体中富含ω-脂肪酸的油。 最理想的是，本发明的LPAAT2的底物特异性将特别适用于在含油酵母例如解脂耶氏酵母中积累具有等于或大于C 20的链长的长链PUFA。

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