公开(公告)号：US20180340170A1

公开(公告)日：2018-11-29

申请号：US15842687

申请日：2017-12-14

Applicant: 10X Genomics, Inc.

Inventor： Kamila Belhocine ,  Geoffrey McDermott ,  Francesca Meschi ,  Xinying Zheng

IPC: C12N15/10

CPC classification number: C12N15/1065 ,  C12N15/1068 ,  C12N15/1075 ,  C12N15/1096 ,  C12Q2563/179

Abstract: Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets) including a single biological particle and a single bead comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions.

公开(公告)号：US20160348093A1

公开(公告)日：2016-12-01

申请号：US15157643

申请日：2016-05-18

Applicant: 10X Genomics, Inc.

Inventor： Andrew D. Price ,  Christopher Hindson ,  Rajiv Bharadwaj ,  Sukhvinder Kaur ,  Geoffrey McDermott

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1006 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q2527/119 ,  C12Q2527/125 ,  C12Q2527/153 ,  C12Q2563/149 ,  C12Q2563/179 ,  C12Q2565/629 ,  C12Q2563/159

Abstract: Compositions that include particle suspensions where such particle suspensions have characteristics for use in a variety of applications including, for example, flow restriction, reagent delivery, and use in microfluidic systems. In some compositions provided, the particle suspension include deformable particles and in particular compositions the deformable particles are beads or gel beads.

Abstract translation: 包括颗粒悬浮液的组合物，其中这种颗粒悬浮液具有用于各种应用的特征，包括例如流动限制，试剂递送和在微流体系统中的应用。 在提供的一些组合物中，颗粒悬浮液包括可变形颗粒，特别是组合物中，可变形颗粒是珠粒或凝胶珠。

公开(公告)号：US20240401028A1

公开(公告)日：2024-12-05

申请号：US18810294

申请日：2024-08-20

Applicant: 10x Genomics, Inc

Inventor： Geoffrey McDermott ,  Andrew Price ,  Michael Schnall-Levin

IPC: C12N15/10

Abstract: Provided herein are systems and methods for processing biomolecules (e.g., nucleic acid molecules, proteins) from a sample. A method for processing biomolecules may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule) and barcoding the probe-nucleic acid molecule complex or derivatives thereof. Such a method can comprise performing a nucleic acid reaction, e.g., extension, denaturation, and amplification. A method for processing a sample may comprise hybridizing probes to (i) target regions of a nucleic acid molecule (e.g., RNA molecule) and (ii) a reporter oligonucleotide of a feature binding group, and barcoding the probe-associated molecules. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well.

公开(公告)号：US12084716B2

公开(公告)日：2024-09-10

申请号：US17473760

申请日：2021-09-13

Applicant: 10X Genomics, Inc.

Inventor： Phillip Belgrader ,  Zachary Bent ,  Rajiv Bharadwaj ,  Vijay Kumar Sreenivasa Gopalan ,  Josephine Harada ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Michael Ybarra Lucero ,  Geoffrey McDermott ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Serge Saxonov ,  John R. Stuelpnagel ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Indira Wu ,  Solongo Batjargal Ziraldo ,  Stephane Claude Boutet ,  Sarah Taylor ,  Niranjan Srinivas

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6874

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2563/179

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US20240254658A1

公开(公告)日：2024-08-01

申请号：US18593788

申请日：2024-03-01

Applicant: 10x Genomics, Inc.

Inventor： Jason Bell ,  Francesca Meschi ,  Geoffrey McDermott ,  Michael Schnall-Levin ,  Xinying Zheng

IPC: C40B30/04 ,  C07K14/74 ,  C12N15/10 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C40B50/06 ,  C40B70/00 ,  G01N33/50 ,  G01N33/53 ,  G01N33/532 ,  G01N33/543 ,  G01N33/548 ,  G01N33/569 ,  G01N33/58

CPC classification number: C40B30/04 ,  C07K14/70539 ,  C12N15/1037 ,  C12N15/1055 ,  C12N15/1065 ,  C12N15/1075 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  G01N33/5032 ,  G01N33/505 ,  G01N33/5304 ,  G01N33/5306 ,  G01N33/5308 ,  G01N33/532 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/548 ,  G01N33/56977 ,  G01N33/58 ,  C12N2310/20 ,  C12N2320/10 ,  C12Q2537/164 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2565/1015 ,  C40B50/06 ,  C40B70/00

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

公开(公告)号：US20240052397A1

公开(公告)日：2024-02-15

申请号：US18339082

申请日：2023-06-21

Applicant: 10x Genomics, Inc.

Inventor： Geoffrey McDermott

IPC: C12Q1/6806

CPC classification number: C12Q1/6806

Abstract: The present disclosure provides methods and systems for nucleic acid preparation and/or analysis. Nucleic acids may be derived from one or more cells. Nucleic acid preparation may comprise generating nucleic acid molecules of varying lengths. Nucleic acid analysis may comprise identifying nucleic acid sequence information with nucleosome position information.

公开(公告)号：US20220033888A1

公开(公告)日：2022-02-03

申请号：US17312247

申请日：2019-12-06

Applicant: 10x Genomics, Inc.

Inventor： Michael Schnall-Levin ,  Eswar Prasad Ramachandran Iyer ,  Yifeng Yin ,  Tarjei Sigurd Mikkelsen ,  Preyas Shah ,  Geoffrey McDermott ,  Adam Lowe ,  Joshua Delaney ,  Augusto Manuel Tentori ,  Rajiv Bharadwaj ,  Stefania Giacomello ,  Alexander Gagnon ,  Meghan L. F. Frey ,  Zachary Bent ,  Erik Leonard Henrik Borgstrom

IPC: C12Q1/6837 ,  C12Q1/6881

Abstract: The present disclosure relates to compositions and methods for generating capture probes on a substrate for identifying the location of analytes in a biological sample.

公开(公告)号：US20210047677A1

公开(公告)日：2021-02-18

申请号：US17003219

申请日：2020-08-26

Applicant: 10X GENOMICS, INC.

Inventor： Darisha Jhutty ,  Geoffrey McDermott

IPC: C12Q1/6806 ,  C12Q1/6844 ,  C12N15/10

Abstract: The present disclosure provides methods and systems for sample processing or analysis. A method for processing a sample may comprise co-partitioning an enzyme and a biological particle comprising a first set of molecules (e.g., RNA molecules comprising one or more mRNA molecules) and a second set of molecules (e.g., RNA molecules other than mRNA molecules) in a partition, lysing or permeabilizing the biological particle to provide access to the first set of molecules and the second set of molecules, and selectively digesting molecules of the second set of molecules with the enzyme to increase a concentration or amount of the first set of molecules relative to the second set of molecules within the partition.

公开(公告)号：US10725027B2

公开(公告)日：2020-07-28

申请号：US16375093

申请日：2019-04-04

Applicant: 10X Genomics, Inc.

Inventor： Jason Bell ,  Geoffrey McDermott ,  Francesca Meschi ,  Michael Schnall-Levin ,  Xinying Zheng

IPC: G01N33/532 ,  G01N33/543 ,  G01N33/548 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12N15/11 ,  G01N33/569 ,  C40B70/00 ,  C07K14/74 ,  C12N15/85

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

公开(公告)号：US20200002763A1

公开(公告)日：2020-01-02

申请号：US16439675

申请日：2019-06-12

Applicant: 10X Genomics, Inc.

Inventor： Phillip Belgrader ,  Zachary Bent ,  Rajiv Bharadwaj ,  Vijay Kumar Sreenivasa Gopalan ,  Josephine Harada ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Michael Ybarra Lucero ,  Geoffrey McDermott ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Serge Saxonov ,  John R. Stuelpnagel ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Indira Wu ,  Solongo Batjargal Ziraldo ,  Stephane Claude Boutet ,  Sarah Taylor ,  Niranjan Srinivas

IPC: C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6806

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.