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公开(公告)号：US20180264468A1
公开(公告)日：2018-09-20
申请号：US15760984
申请日：2016-09-16
Inventor： Clifford Anderson , Kristen Lee , Jacob Messner , Laimonas Kelbauskas , Benjamin Ueberroth , Yanqing Tian , Deirdre Meldrum
IPC: B01L3/00
CPC classification number: B01L3/50853 , B01L2200/025 , B01L2200/0689 , B01L2200/141 , B01L2200/142 , B01L2300/0627 , B01L2300/0829 , B01L2300/123 , B01L2300/16
Abstract: A multi-layer sealing structure for sealing a microwell array defined in or on a substrate includes at least one front compliant layer, a back compliant layer, and a flexural layer arranged between the at least one front compliant layer and the back compliant layer, wherein the at least one front compliant layer is closer than the back compliant layer to microwells of the microwell array. One or more front compliant layers may be optically reflective and/or may embody a sensor layer. The back compliant layer may include an adhesive or various types of rubber, and the flexural layer may include a polymeric material or metal. A multi-layer sealing structure may be separated from a microwell array by peeling. A multi-layer sealing structure allows local disruption of sealing where particle contaminants are present without compromising the sealing performance of an entire microwell array, and without requiring a large sealing force.
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公开(公告)号：US09410970B2
公开(公告)日：2016-08-09
申请号：US14533349
申请日：2014-11-05
Inventor： Yanqing Tian , Liqiang Zhang , Fengyu Su , Deirdre Meldrum , Sean Buizer , Clifford L. Anderson , Laimonas Kelbauskas , Kristen Lee
CPC classification number: G01N33/84 , G01N21/6428 , G01N33/66
Abstract: A triple sensor structured for simultaneous measurement of glucose, oxygen, and pH. The sensor components are in thin film states such as sensing films or membranes, with a glucose probe associated with emission of radiation in the blue part of the spectrum, an oxygen probe associated with radiation in red portion of the spectrum, and a pH probe—with a green portion of the spectrum. The optical probes are chemically grafted or immobilized in a suitable polymer matrix, alleviating the leaching of the probes from the matrix, improving the thin film sensing stability, and enabling the repeatable use of the same sensing films.
Abstract translation: 三重传感器，用于同时测量葡萄糖，氧气和pH值。 传感器组件处于薄膜状态，例如感测膜或膜，葡萄糖探针与光谱蓝色部分中的辐射相关，与光谱红色部分的辐射相关的氧探针，以及pH探针 - 具有绿色部分的光谱。 光学探针被化学接枝或固定在合适的聚合物基质中，减轻了探针从基体中的浸出，改善了薄膜感测的稳定性，并使得能够重复使用相同的传感膜。
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公开(公告)号：US11136614B2
公开(公告)日：2021-10-05
申请号：US15757712
申请日：2016-10-07
Inventor： Jacob Messner , Clifford Anderson , Honor Glenn , Kristen Lee , Mark Richards , Laimonas Kelbauskas , Kimberly Bussey , Deirdre Meldrum
IPC: C12Q1/24 , C12M1/00 , C12M1/32 , G01N33/569
Abstract: Methods for seeding live cells onto spatially defined regions of a substrate including multiple features (e.g., microwells or other microenvironments) utilize a stencil embodied in a hole-defining sacrificial film. A sacrificial film devoid of holes may be applied over features of a substrate, and a hole generating mechanism (e.g., hot needle or laser) aligned with features may be used to define holes in the film. Alternatively, holes may be predefined in a sacrificial film to form a stencil, and the stencil may be assembled to the substrate with the holes registered with features thereof. Thereafter, cells are seeded through holes in the film. Seeded cells are subject to incubation, further processing, and/or performance of one or more assays, and the hole-defining sacrificial film (stencil) may be removed.
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Patent Agency Ranking
公开(公告)号：US20200178967A1
公开(公告)日：2020-06-11
申请号：US16631040
申请日：2018-08-29
Inventor： Deirdre Meldrum , Fengyu Su , Laimonas Kelbauskas , Vivek Nandakumar , Yanqing Tian , Roger Johnson
Abstract: A thixotropic and biocompatible gel, formed by combining of 4-arm polyethylene glycol with fumed silica (PEG-silica). The thixotropicity of the gel is affected by the ratio of PEG to silica. To study the gels biocompatibility, the cell suspension in PBS or another medium was added to the PEG-silica gel via mixing to uniformly disperse the cells in the gel and then placed in an incubator before performing fluorescence live-dead assays. Duration of cell viability (cell life time) in gels was measured to be up to several days, depending on gel composition. Due to their optical and fluidic properties the gels are compatible with live cell imaging including 3D computed tomography and offer a means for moving the cells in a highly controllable manner by applying and removing pressure on the gels.
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公开(公告)号：US10246726B2
公开(公告)日：2019-04-02
申请号：US15938742
申请日：2018-03-28
Inventor： Bo Wang , Weiwen Zhang , Deirdre Meldrum , David Nielsen
Abstract: Construction and expression of synthetic pathways to produce (S) or (R)-3-hydroxybutyrate (3HB) as enantiomerically-pure products by genetically engineering cyanobacterium Synechocystis sp. PCC 6803. Under optimized growth conditions, the pathway employing phaA and phaB from R. eutropha was the most effective, producing up to 533.4±5.5 mg/l (R)-3HB after 21 days photosynthetic cultivation. For the first time, the feasibility and high efficiency of producing 3HB using solar energy and CO2 as sole energy and carbon sources by engineered cyanobacteria is demonstrated.
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公开(公告)号：US10221443B2
公开(公告)日：2019-03-05
申请号：US15135150
申请日：2016-04-21
Inventor： Deirdre Meldrum , Shih-Hui (Joseph) Chao , Thai Tran , Laimonas Kelbauskas , Jeff Houkal , Andrew Hatch , Weimin Gao , David Richardson
IPC: C12Q1/68 , C12Q1/6806 , C12M1/00 , G01N1/28 , C12Q1/6848 , B01L3/02 , B01L3/00 , C12Q1/6841 , C12Q1/686
Abstract: Systems and methods for in situ laser lysis for analysis of biological tissue (live, fixed, frozen or otherwise preserved) at single cell resolution in 3D. For example, a system and method for lysing individual cells in situ, including the steps of capturing a tissue sample comprising a cellular content, subjecting the tissue sample to a stream of continuous fluid flow, lysing a selected area of the tissue sample with a laser, thereby releasing at least a portion of the cellular content from the tissue sample, recovering at least one target molecule from the cellular content in the stream, and processing at least one target molecule is provided. The system collects cellular contents, performs highly multiplexed (RT-qPCR or RNA-seq), and sequentially (cell-by-cell) reconstructs a 3D spatial map of mRNA expression of the tissue with a large number of genes. A 3D spatial map of the DNA, RNA, and/or proteins can be generated for each cell in the tissue.
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公开(公告)号：US20180312884A1
公开(公告)日：2018-11-01
申请号：US15938742
申请日：2018-03-28
Inventor： Bo Wang , Weiwen Zhang , Deirdre Meldrum , David Nielsen
CPC classification number: C12P7/52 , C12N9/0006 , C12N9/1029 , C12N9/16 , C12N15/52 , C12Y101/01036 , C12Y101/01157 , C12Y203/01009 , C12Y301/0202 , Y02P20/134
Abstract: Construction and expression of synthetic pathways to produce (S) or (R)-3-hydroxybutyrate (3HB) as enantiomerically-pure products by genetically engineering cyanobacterium Synechocystis sp. PCC 6803. Under optimized growth conditions, the pathway employing phaA and phaB from R. eutropha was the most effective, producing up to 533.4±5.5 mg/l (R)-3HB after 21 days photosynthetic cultivation. For the first time, the feasibility and high efficiency of producing 3HB using solar energy and CO2 as sole energy and carbon sources by engineered cyanobacteria is demonstrated.
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公开(公告)号：US10022718B2
公开(公告)日：2018-07-17
申请号：US14922696
申请日：2015-10-26
Inventor： Rhett Martineau , Jeff Houkal , Shih-Hui Chao , Weimin Gao , Shufang Ci , Deirdre Meldrum
Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.
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