公开(公告)号：US20230348833A1

公开(公告)日：2023-11-02

申请号：US18206501

申请日：2023-06-06

Applicant: CORNING INCORPORATED

Inventor： Ann MeeJin Ferrie ,  Vasiliy Nikolaevich Goral ,  Lori Eileen Romeo

IPC: C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12M1/12

CPC classification number: C12M25/14 ,  C12M23/02 ,  C12M23/20 ,  C12M23/44 ,  C12M25/04 ,  C12M29/10 ,  C12M29/26 ,  C12M41/26 ,  C12N5/0068

Abstract: A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.

公开(公告)号：US10808221B2

公开(公告)日：2020-10-20

申请号：US15537111

申请日：2015-12-16

Applicant: Corning Incorporated

Inventor： Ye Fang ,  Ann MeeJin Ferrie ,  Vasiliy Nikolaevich Goral ,  David Henry ,  Martial Hervy ,  Corinne Walerack ,  Yue Zhou

IPC: C12N5/00 ,  C08J3/24 ,  C09D167/00

Abstract: A cell culture article includes a substrate having a polymer coating that is conducive to colony passaging of cells cultured on the coating. Example polymer coatings are formed from polygalacturonic acid (PGA), alginate, or combinations thereof. Cells cultured on the polymer coating can be separated from the substrate as a colony or layer of cells by exposing the polymer coating to (i) a chelating agent, (ii) a proteinase-free enzyme, or (iii) a chelating agent and a proteinase-free enzyme.

公开(公告)号：US20200248120A1

公开(公告)日：2020-08-06

申请号：US16781685

申请日：2020-02-04

Applicant: CORNING INCORPORATED

Inventor： Ann MeeJin Ferrie ,  Vasiliy Nikolaevich Goral ,  Lori E Romeo

IPC: C12M1/12 ,  C12M1/00

Abstract: A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.

公开(公告)号：US10319502B2

公开(公告)日：2019-06-11

申请号：US15520581

申请日：2015-10-23

Applicant: CORNING INCORPORATED

Inventor： Ann MeeJin Ferrie ,  Yan Jin ,  Lingyan Wang ,  Yue Zhou

IPC: H01F1/12 ,  H01F1/00 ,  C12N15/10 ,  H01F1/34 ,  H01F1/36 ,  H01F1/20

Abstract: Magnetic particles (100) have a particle size (134) of 500 nm or less and include a core (110) and a polymer coating (120) that surrounds and encapsulates the core (110). The core (110) includes a metal, metal alloy, or metal oxide of at least one metal such as B, Mg, Al, Mn, Co, Ni, Cu, Fe Sm, Yb, Dy, Gd or Er and Nb. The magnetic core (100) is a polycrystalline particle and is a superspin glass magnetic material, having a coercivity greater than zero and a magnetic remenance greater than zero at room temperature. Above room temperature and at low field, the magnetic moment of these superspin glass magnetic materials increases with temperature. An in situ hydrolysis/precipitation method from precursor metal salts is used to form the polymer-encapsulated magnetic particles (100).

公开(公告)号：US09976230B2

公开(公告)日：2018-05-22

申请号：US14853191

申请日：2015-09-14

Applicant: CORNING INCORPORATED

Inventor： Glen Bennett Cook ,  Ann MeeJin Ferrie ,  Shawn Michael O'Malley ,  Vitor Marino Schneider

IPC: C30B13/00 ,  C30B29/20 ,  C23C18/12 ,  C03C17/25

CPC classification number: C30B13/00 ,  C03C17/25 ,  C03C2217/214 ,  C03C2218/113 ,  C03C2218/116 ,  C03C2218/32 ,  C23C18/1216 ,  C23C18/1241 ,  C23C18/1245 ,  C23C18/1254 ,  C23C18/1295 ,  C30B29/20

Abstract: A crystallized layer can be formed on a substrate from a precursor layer deposited on a surface of the substrate. The precursor layer can be an oxide, a nitride, a carbide, or an oxynitride. The process for forming the crystallized layer includes melting the precursor layer formed on the surface of the substrate by localized topical heating of the precursor layer and then cooling the melted precursor layer so that it crystallized to form a scratch resistant crystallized layer. The scratch resistant crystallized layer can have a hardness of 15 GPa or greater.

公开(公告)号：US20170226455A1

公开(公告)日：2017-08-10

申请号：US15499370

申请日：2017-04-27

Applicant: Corning Incorporated

Inventor： Ye Fang ,  Ann MeeJin Ferrie ,  Vasiliy Nikolaevich Goral ,  Allison Jean Tanner ,  Qi Wu

IPC: C12M1/32 ,  C12N5/00

CPC classification number: C12M23/12 ,  C12M41/36 ,  C12N5/0062

Abstract: A cell culture apparatus may include a substrate defining a well. The well may define an interior surface, an exterior surface, an upper aperture and a nadir. The substrate may define a thickness between the interior and exterior surfaces that has a thickness proximate the nadir that is greater than or equal to a thickness proximate the upper aperture.

公开(公告)号：US12203059B2

公开(公告)日：2025-01-21

申请号：US17532196

申请日：2021-11-22

Applicant: CORNING INCORPORATED

Inventor： Ye Fang ,  Ann MeeJin Ferrie ,  Vasiliy Nikolaevich Goral ,  Allison Jean Tanner ,  Qi Wu

IPC: C12M1/00 ,  C12M1/32 ,  C12M1/34 ,  C12N5/00

Abstract: A cell culture apparatus may include a substrate defining a well. The well may define an interior surface, an exterior surface, an upper aperture and a nadir. The substrate may define a thickness between the interior and exterior surfaces that has a thickness proximate the nadir that is greater than or equal to a thickness proximate the upper aperture.

公开(公告)号：US12116556B2

公开(公告)日：2024-10-15

申请号：US18382827

申请日：2023-10-23

Applicant: CORNING INCORPORATED

Inventor： Ann MeeJin Ferrie ,  Vasiliy Nikolaevich Goral

IPC: C12M1/12 ,  C12M1/00 ,  C12M3/00

CPC classification number: C12M25/02 ,  C12M23/02 ,  C12M23/20 ,  C12M23/44 ,  C12M25/14 ,  C12M29/10 ,  C12M29/26

Abstract: A fixed-bed bioreactor system is provided that includes a vessel with a media inlet, a media outlet, and an interior cavity disposed between and in fluid communication with the media inlet and media outlet. The vessel further includes a cell culture substrate disposed in the interior cavity between the media inlet and the media outlet in a packed-bed configuration, the cell culture substrate including a plurality of porous disks in a stacked arrangement. The interior cavity includes a cell culture section and a spacer section, the cell culture substrate defining the cell culture section and the spacer section being disposed between the cell culture section and the media outlet, and each of the plurality of porous disks has a surface configured to culture cells thereon.

公开(公告)号：US20240043781A1

公开(公告)日：2024-02-08

申请号：US18382827

申请日：2023-10-23

Applicant: CORNING INCORPORATED

Inventor： Ann MeeJin Ferrie ,  Vasiliy Nikolaevich Goral

IPC: C12M1/12 ,  C12M1/00 ,  C12M3/00

CPC classification number: C12M25/02 ,  C12M23/02 ,  C12M23/20 ,  C12M23/44 ,  C12M25/14 ,  C12M29/10 ,  C12M29/26

Abstract: A fixed-bed bioreactor system is provided that includes a vessel with a media inlet, a media outlet, and an interior cavity disposed between and in fluid communication with the media inlet and media outlet. The vessel further includes a cell culture substrate disposed in the interior cavity between the media inlet and the media outlet in a packed-bed configuration, the cell culture substrate including a plurality of porous disks in a stacked arrangement. The interior cavity includes a cell culture section and a spacer section, the cell culture substrate defining the cell culture section and the spacer section being disposed between the cell culture section and the media outlet, and each of the plurality of porous disks has a surface configured to culture cells thereon.

公开(公告)号：US20230235262A1

公开(公告)日：2023-07-27

申请号：US18130668

申请日：2023-04-04

Applicant: CORNING INCORPORATED

Inventor： Ann MeeJin Ferrie ,  Vasiliy Nikolaevich Goral

IPC: C12M1/12 ,  C12M1/00 ,  C12M3/00 ,  C12M1/34

CPC classification number: C12M25/14 ,  C12M23/02 ,  C12M23/44 ,  C12M25/04 ,  C12M29/10 ,  C12M41/26 ,  C12M23/20 ,  C12M29/26 ,  C12N5/0068

Abstract: A bioreactor system is provided that includes a cell culture vessel having a first end, a second end, and at least one reservoir between the first and second ends; and a cell culture matrix disposed in the at least one reservoir. The cell culture matrix has a structurally defined substrate with a surface for adhering cells thereto. The bioreactor system flows material through the at least one reservoir and through the cell culture matrix in a flow direction from the first end to the second end, and the cell culture matrix exhibits isotropic fluid flow permeability therethrough.