-
公开(公告)号：US07815783B2
公开(公告)日：2010-10-19
申请号：US11073999
申请日：2005-03-08
Applicant: Egisto Boschetti , Lee O. Lomas , Pierre Girot
Inventor： Egisto Boschetti , Lee O. Lomas , Pierre Girot
IPC: G01N27/453 , B01D57/02
CPC classification number: G01N27/44704 , B01D57/02 , B01J20/3204 , B01J20/327 , B01J20/3272 , B01J20/3274 , B01J2220/54 , C07K1/24 , C07K1/34 , G01N27/44795
Abstract: Each embodiment includes a central sample reservoir and a plurality of satellite reservoirs. In a first embodiment, a first electrode in electrical contact with the central reservoir is charged and second electrodes in electrical contact with the satellite reservoirs are sequentially charged, thereby pI filtering molecules in the central reservoir into the satellite reservoirs. In a second embodiment, the central reservoir is configured to rotate so that molecules in a sample in the central reservoir are centrifugally pI-filtered into the satellite reservoirs. In a third embodiment, first and second electrodes proximate opposite first and second satellite reservoirs, respectively, are charged. Some molecules in a sample are pI filtered into the first and second satellite reservoirs. Third and fourth electrodes proximate opposite third and fourth satellite reservoirs, respectively, are then charged. Some molecules in a sample are pI filtered into the third and fourth satellite reservoirs.
Abstract translation: 每个实施例包括中央样品储存器和多个卫星储存器。 在第一实施例中，与中央储存器电接触的第一电极被充电，并且与卫星储存器电接触的第二电极被顺序地充电，从而将中央储存器中的分子过滤到卫星储存器中。 在第二实施例中，中央储存器被配置为旋转，使得中央储存器中的样品中的分子被离心地pI过滤到卫星储存器中。 在第三实施例中，分别与第一和第二卫星储存器相邻的第一和第二电极被充电。 样品中的一些分子被pI过滤到第一和第二卫星储层中。 然后分别靠近相对的第三和第四卫星储存器的第三和第四电极被充电。 样品中的一些分子被pI过滤到第三和第四卫星储层中。
-
公开(公告)号：US07348184B2
公开(公告)日：2008-03-25
申请号：US10626493
申请日：2003-07-23
Applicant: William E. Rich , Egisto Boschetti , Lee O. Lomas , Tai-Tung Yip
Inventor： William E. Rich , Egisto Boschetti , Lee O. Lomas , Tai-Tung Yip
IPC: G01N33/543
CPC classification number: C40B30/04 , G01N33/54306 , G01N33/6803
Abstract: This invention provides methods and materials for mapping interaction characteristics between components of a multicomponent biological complex. The methods involve capturing a multicomponent complex on a solid support and washing the support with a series of elution washes forming a gradient of solute concentrations, and determining whether a particular elution wash eluted a particular component.
Abstract translation: 本发明提供了用于映射多组分生物复合物的组分之间的相互作用特征的方法和材料。 该方法包括在固体支持物上捕获多组分复合物，并用洗脱洗涤洗涤洗脱洗涤洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱特定组分。
-
23.发明申请PREPARATION AND USE OF MIXED MODE SOLID SUBSTRATES FOR CHROMATOGRAPHY ADSORBENTS AND BIOCHIP ARRAYS 有权混合模式固体基质的制备和使用用于色谱吸附和生物体阵列公开(公告)号：US20070015143A1
公开(公告)日：2007-01-18
申请号：US11531863
申请日：2006-09-14
Applicant: Egisto Boschetti , Pierre Girot
Inventor： Egisto Boschetti , Pierre Girot
CPC classification number: B01J20/3255 , B01D15/1807 , B01J20/286 , B01J20/289 , B01J20/3217 , B01J20/3219 , B01J20/3242 , B01J20/3285 , B01J2219/00527 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00702 , B01J2219/00725 , B01J2219/0074 , C07K1/165 , C07K1/22 , C40B40/10 , C40B80/00 , G01N33/54353 , G01N2001/045 , G01N2035/00158 , Y10S530/812 , Y10T436/25
Abstract: For certain mixed mode resins having anionic character, a ligand is joined to a solid support via a linkage that includes a mercapto-, ether- or amino-containing moiety. A suitable ligand comprises an aromatic group, a heteroaromatic group, or a heterocyclic group, optionally fused, that is sulfate-, sulfonate-, phosphonate- or phosphate-substituted and that is linked to such a moiety. These resins possess an anionic character under conditions prescribed for their use. Separation of a biological substance, such as a peptide or protein, can be accomplished with a resin of this type via a change in the pH of eluants, thereby effecting adsorption and desorption.
Abstract translation: 对于具有阴离子特性的某些混合模式树脂，配体通过包含巯基，醚或氨基部分的键连接到固体支持物上。 合适的配体包括芳族基团，杂芳族基团或杂环基团，任选地稠合，其是硫酸酯基，磺酸酯基，膦酸酯基或磷酸酯取代的，并且与该部分连接。 这些树脂在其使用条件下具有阴离子特性。 生物物质如肽或蛋白质的分离可以通过这种类型的树脂通过洗脱液的pH的变化来实现，从而进行吸附和解吸附。
-
公开(公告)号：US20060205010A1
公开(公告)日：2006-09-14
申请号：US10553923
申请日：2004-04-19
Applicant: Catherine Allioux , Egisto Boschetti , Virginie Brenac , Patrick Santambien , James Spencer
Inventor： Catherine Allioux , Egisto Boschetti , Virginie Brenac , Patrick Santambien , James Spencer
IPC: G01N33/53
CPC classification number: G01N33/567 , C07K1/22 , G01N33/543 , G01N33/6803 , G01N33/6842 , G01N33/6851
Abstract: The invention provides methods of determining the presence of host cell proteins in sample. The invention further provides methods of following the purification of target proteins. Kits and articles of manufacture are also provided.
Abstract translation: 本发明提供了确定样品中宿主细胞蛋白质存在的方法。 本发明还提供了追踪靶蛋白纯化的方法。 还提供了工具包和制品。
-
25.发明申请Colloidal metal labeled microparticles and methods for producing and using the same 审中-公开胶体金属标记的微粒及其制备和使用方法公开(公告)号：US20050158393A1
公开(公告)日：2005-07-21
申请号：US11030182
申请日：2005-01-07
Applicant: Phillippe Reb , Laurent Domas , Egisto Boschetti , Marie-Paule Leroy-Landercy
Inventor： Phillippe Reb , Laurent Domas , Egisto Boschetti , Marie-Paule Leroy-Landercy
CPC classification number: A61K49/18
Abstract: The present invention relates to polymeric materials that are labeled with colloidal metals, preferably colloidal gold, to processes for producing the labeled polymeric material, and to methods of using the materials in prophylactic, therapeutic and cosmetic applications. Specifically, the invention relates to porous injectable and implantable microparticles, preferably microspheres, that are associated with colloidal metals such that the microparticles are visible or detectable under regular light, by radiological and/or magnetic resonance imaging techniques, or both. The microparticles having colloidal metals are particularly useful for embolization, dermal augmentation and tissue bulking, drug delivery, gene therapy, and other prophylactic, therapeutic or cosmetic medical applications.
Abstract translation: 本发明涉及用胶体金属（优选胶体金）标记用于生产标记的聚合物材料的方法的聚合物材料，以及在预防，治疗和美容应用中使用该材料的方法。 具体地，本发明涉及与胶体金属相关的多孔可注射和可植入的微粒，优选微球，使得微粒在常规光下是可见的或可检测的，通过放射和/或磁共振成像技术，或两者兼有。 具有胶体金属的微粒特别可用于栓塞，皮肤增生和组织膨胀，药物递送，基因治疗和其它预防，治疗或美容医学应用。
-
Patent Agency Ranking
26.发明授权Large pore volume composite mineral oxide beads, their preparation and their applications for adsorption and chromatography 失效大孔体积复合矿物氧化物珠，其制备及其在吸附和色谱上的应用公开(公告)号：US06613234B2
公开(公告)日：2003-09-02
申请号：US09274013
申请日：1999-03-22
Applicant: Nicolas Voute , Egisto Boschetti , Pierre Girot , Marc Perez , Jean-Claude Viguie
Inventor： Nicolas Voute , Egisto Boschetti , Pierre Girot , Marc Perez , Jean-Claude Viguie
IPC: B01D1508
CPC classification number: B01J20/3204 , B01D15/1807 , B01D15/327 , B01D15/361 , B01D15/3804 , B01J20/0211 , B01J20/06 , B01J20/08 , B01J20/28069 , B01J20/28085 , B01J20/28097 , B01J20/282 , B01J20/3064 , B01J20/3272 , B01J20/3274 , B01J20/3282 , B01J2220/52
Abstract: The present invention provides porous mineral oxide beads which have large pore volumes and enhanced stability. The beads are based on a tetravalent metal oxide, such as zirconia, titania or hafnia. These highly porous beads are produced from a mixture of tetravalent mineral oxides, mineral pore inducing agents which are oxides or salts of trivalent metals, and optional binders. The porous mineral beads can be filled with a polymer gel and used for adsorption and chromatography applications.
Abstract translation: 本发明提供了具有大孔体积和增强的稳定性的多孔无机氧化物珠粒。 珠粒基于四价金属氧化物，例如氧化锆，二氧化钛或铪。 这些高度多孔的珠子由四价矿物氧化物，矿物孔诱导剂，三价金属的氧化物或盐，和任选的粘合剂的混合物制成。 多孔矿物珠可以用聚合物凝胶填充并用于吸附和色谱应用。
-
公开(公告)号：US06436424B1
公开(公告)日：2002-08-20
申请号：US09528990
申请日：2000-03-20
Applicant: Jean-Marie Vogel , Egisto Boschetti
Inventor： Jean-Marie Vogel , Egisto Boschetti
IPC: A61F1300
Abstract: The present invention relates to injectable compositions comprising biocompatible, swellable, hydrophilic, non-toxic and substantially spherical microspheres microspheres and a biocompatible carrier for use in dermal augmentation. The present invention further relates to methods of dermal augmentation, particularly for the treatment of skin contour deficiencies, using the injectable compositions.
Abstract translation: 本发明涉及可注射组合物，其包含生物相容性，可溶胀，亲水，无毒和基本上为球形的微球微球和用于皮肤增生的生物相容性载体。 本发明还涉及使用可注射组合物的皮肤增强方法，特别是用于治疗皮肤轮廓缺陷的方法。
-
公开(公告)号：US5906734A
公开(公告)日：1999-05-25
申请号：US858924
申请日：1997-05-19
Applicant: Pierre Girot , Egisto Boschetti
Inventor： Pierre Girot , Egisto Boschetti
CPC classification number: B01D15/08 , B01D15/20 , B01J20/28004 , B01J20/28057 , B01J20/28069 , B01J20/28078 , B01J20/288 , B01J20/3204 , B01J20/321 , B01J20/327 , B01J20/3282 , B01J20/3289 , B01J39/26 , B01J41/20 , B01J2220/54 , B01J2220/58
Abstract: This invention relates generally to modified porous solid supports and processes for the preparation and use of same. In particular, passivated porous supports are disclosed which are characterized by a reversible high sorptive capacity substantially unaccompanied by non-specific adsorption of or interaction with biomolecules. Passivation is achieved by use of a passivation mixture comprising a main monomer, a passivating agent comprising polyethyleneimine, and a crosslinking agent, which mixture upon polymerization results in the substantial elimination of the undesirable non-specific interaction with biomolecules.
-
公开(公告)号：US5672276A
公开(公告)日：1997-09-30
申请号：US720799
申请日：1996-10-01
Applicant: Pierre Girot , Egisto Boschetti
Inventor： Pierre Girot , Egisto Boschetti
CPC classification number: B01D15/20 , B01J20/28004 , B01J20/28057 , B01J20/28069 , B01J20/28078 , B01J20/288 , B01J20/3204 , B01J20/321 , B01J20/327 , B01J20/3282 , B01J20/3289 , B01J39/165 , B01D15/3804 , B01J2220/54 , B01J2220/58
Abstract: This invention relates generally to modified porous solid supports and processes for the preparation and use of same. In particular, passivated porous polymeric supports are disclosed which are characterized by a reversible high sorptive capacity substantially unaccompanied by non-specific adsorption of or interaction with biomolecules. Passivation is achieved by use of a passivation mixture comprising a main monomer, a passivating monomer that is different from the main monomer, and a crosslinking agent, which mixture upon polymerization results in the substantial elimination of the undesirable non-specific interaction with biomolecules.
-
30.发明授权Porous support for the removal of small exogenous molecules from biological fluids 失效多孔支持从生物流体中去除小的外源性分子
公开(公告)号：US5609763A
公开(公告)日：1997-03-11
申请号：US564994
申请日：1995-11-30
Applicant: Egisto Boschetti , Pierre Girot , Luc P. Guerrier
Inventor： Egisto Boschetti , Pierre Girot , Luc P. Guerrier
CPC classification number: B01J20/3282 , B01D15/00 , B01J20/28004 , B01J20/28057 , B01J20/28069 , B01J20/28078 , B01J20/28097
Abstract: Novel sorbents and methods for removing small hydrophobic and amphophilic molecules from biological fluids were disclosed. The methods and materials were particularly useful for removing viral inactivating agents from blood and blood fractions. The novel sorbents comprise a porous mineral oxide matrix having its interior porous volume substantially filled with a crosslinked hydrophobic polymer network.
Abstract translation: 公开了从生物液体中去除小疏水和两性分子的新型吸附剂和方法。 所述方法和材料特别可用于从血液和血液部分中除去病毒灭活剂。 新型吸附剂包括多孔矿物氧化物基质，其基本上填充有交联的疏水性聚合物网络的内部多孔体积。
-
-
-
-
-
-
-
-
-
Search Results
90
records
(took 0.026 seconds)
