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公开(公告)号：US08092683B2
公开(公告)日：2012-01-10
申请号：US12517570
申请日：2008-01-10
IPC分类号： B01D15/08
CPC分类号： C40B30/04 , B01D15/1885 , B01D15/361 , B01D15/362 , B01D15/363 , B01D15/3847 , B01J20/3285 , B01J39/26 , B01J41/20 , B01J2220/54 , C40B40/14 , C40B50/00 , G01N30/466 , G01N30/96
摘要： The present invention relates to a method of preparing a library of resins which are useful in chromatography, which method comprises creating a diversity of multi-modal ion exchange resins; and providing the diversity in a parallel system in which each resin is presented separated from the other resin(s).
摘要翻译： 本发明涉及一种制备可用于色谱法的树脂文件库的方法，该方法包括产生多种多模式离子交换树脂; 并且在与其它树脂分离出每种树脂的并行系统中提供多样性。
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公开(公告)号：US20100121035A1
公开(公告)日：2010-05-13
申请号：US12594002
申请日：2008-03-20
CPC分类号： B01J20/289 , B01D15/32 , B01D15/327 , B01D15/361 , B01D15/3804 , B01D15/3847 , B01J20/262 , B01J20/267 , B01J20/286 , B01J20/3212 , B01J20/3219 , B01J20/3251 , B01J20/3272 , B01J20/328 , B01J20/3285 , B01J2220/54 , B01J2220/58 , C07K1/20 , C07K16/00
摘要： The present invention relates to a separation matrix comprised of a porous or non-porous support to which ligands have been immobilised, wherein said ligands comprise at least one aliphatic sulfamide. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulins, such as IgG, Fab fragments, fusion proteins containing immunoglobulins etc, by adsorption to a separation matrix that comprises the aliphatic sulfamide ligands of the invention.
摘要翻译： 本发明涉及由已经固定有配体的多孔或无孔载体组成的分离基质，其中所述配体包含至少一个脂族磺酰胺。 本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到包含脂族磺酰胺配体的分离基质上分离免疫球蛋白如IgG，Fab片段，含有免疫球蛋白的融合蛋白等的方法 的本发明。
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公开(公告)号：US20060175258A1
公开(公告)日：2006-08-10
申请号：US10547570
申请日：2004-03-05
CPC分类号： B01J20/3285 , B01D15/325 , B01D15/327 , B01J20/286
摘要： The invention is a method of preparing multimodal ligands for hydrophobic interaction chromatography (HIC), which comprises providing a cyclic scaffold comprising a thiol, an amine and a carbonyl group; derivatisation of the nitrogen with a reagent to introduce a primary interaction; and aminolysis of the resulting derivative, whereby a secondary interaction is introduced next to the carbonyl; wherein at least one of the primary interaction and the secondary interaction comprises a hydrophobic group and wherein non of said interactions comprises charged groups, i.e. ion exchange ligands. The invention also encompasses a separation medium comprising such multimodal ligands immobilised to a base matrix.
摘要翻译： 本发明是制备用于疏水相互作用色谱（HIC）的多峰配体的方法，其包括提供包含硫醇，胺和羰基的环状支架; 用试剂衍生氮以引入主要相互作用; 和所得衍生物的氨解，由此在羰基旁边引入二次相互作用; 其中所述主要相互作用和所述第二相互作用中的至少一种包含疏水基团，并且其中所述相互作用中的非相互作用包括带电基团，即离子交换配体。 本发明还包括一种分离介质，其包含固定在基础基质上的这种多峰配体。
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公开(公告)号：US06702943B1
公开(公告)日：2004-03-09
申请号：US10130958
申请日：2002-09-16
IPC分类号： B01D1508
CPC分类号： B01J41/20 , B01D15/327 , B01J41/09 , C02F1/42
摘要： A method for the removal of a substance carrying a negative charge and being present in an aqueous liquid (I). The method comprises the steps of: (i) contacting the liquid with a matrix carrying a plurality of ligands comprising a positively charged structure and a hydrophobic structure, and (ii) desorbing the substance. The characterizing feature is that (I) each of said ligands together with a spacer has the formula: —SP—[Ar—R1—N+(R2R3R4)] where (A) [Ar—R1—N+(R2R3R4)] represents a ligand a) Ar is an aromatic ring, b) R1 is [(L)nR′1]m where n and m are integers selected amongst zero or 1; L is amino nitrogen, ether oxygen or thioether sulphur; R′1 is a linker selected among 1) hydrocarbon groups; 2) —C(═NH)—; c) R2-4 are selected among hydrogen and alkyls; (B) SP is a spacer providing a carbon or a heteroatom directly attached to Ar—R1—N+(R2R3R4); (C)—represents that SP replaces a hydrogen in (Ar—R1—N+(R2R3R4); (D)—represents binding to the matrix; and (II) desorption. There is also described (a) anion-exchangers having high breakthrough capacities, (b) a screening method and (c) a desalting protocol.
摘要翻译： 一种用于去除携带负电荷并存在于水性液体（I）中的物质的方法。 该方法包括以下步骤：（i）使液体与承载包含带正电结构和疏水结构的多个配体的基质接触，和（ii）解吸该物质。 其特征在于：（I）每个所述配体与间隔基一起具有下式：-SP- [Ar-R1-N +（R2R3R4）]其中（A）[Ar-R1-N +（ R2R3R4）]表示配体a）Ar是芳环，b）R1是[（L）nR'1] m，其中n和m是选自零或1的整数; L为氨基氮，醚氧或硫醚硫; R'1是选自1）烃基的连接体; 2）-C（= NH） - ; c）R2-4选自氢和烷基; （B）SP是提供直接与Ar-R 1 -N +（R 2 R 3 R 4）连接的碳或杂原子的间隔基; （C） - 表示SP替代（Ar-R1-N +（R2R3R4）中的氢;（D） - 表示与基质的结合;和（II）解吸，还描述了（a）阴离子交换 具有较高的突破能力，（b）筛选方法和（c）脱盐方案。
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公开(公告)号：US07879244B1
公开(公告)日：2011-02-01
申请号：US10148032
申请日：2000-11-22
IPC分类号： B01D15/00
CPC分类号： B01J41/20 , B01D15/327 , B01J41/09 , C02F1/42
摘要： A method for the removal of a substance, which has a negative charge and which is present in an aqueous liquid (I). The method comprises the steps of: (i) contacting the liquid with an anion-exchanger (1) that comprises mixed mode anion-exchanging ligands in which there is a positively charged nitrogen allowing binding of the substance to the anion-exchanger; and (ii) desorbing said substance from said anion-exchanger. The characteristic feature is that (A) the mixed mode ligands have a thioether linkage within a distance of 1-7 atoms from their positively charged atom, and (B) the anion-exchanger (1) (i) is capable of binding the substance of interest in an aqueous reference liquid (II) at an ionic strength corresponding to 0.25 M NaCl, and (ii) permits in the pH interval 2-12 a maximal breakthrough capacity for the substance which is ≧200% of the breakthrough capacity of the substance for Q-Sepharose Fast Flow (anion-exchanger 2).
摘要翻译： 一种具有负电荷且存在于水性液体（I）中的物质的去除方法。 该方法包括以下步骤：（i）使液体与阴离子交换剂（1）接触，阴离子交换剂（1）包含混合阴离子交换配体，其中带有带正电荷的氮，允许物质与阴离子交换剂结合; 和（ii）从所述阴离子交换器中解吸所述物质。 其特征是（A）混合模式配体在其带正电荷的原子之间具有1-7个原子的距离内的硫醚键，（B）阴离子交换器（1）（i）能够结合物质 在对应于0.25M NaCl的离子强度的水性参考液体（II）中感兴趣的，以及（ii）允许在pH间隔2-12中物质的最大穿透能力为≥200％的穿透能力的物质 Q-Sepharose Fast Flow（阴离子交换器2）的物质。
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公开(公告)号：US07750129B2
公开(公告)日：2010-07-06
申请号：US10589717
申请日：2005-02-25
申请人： Bo-Lennart Johansson , Hans J. Johansson , Anna Grönberg , Jean-Luc Maloisel , Nicolas Thevenin
发明人： Bo-Lennart Johansson , Hans J. Johansson , Anna Grönberg , Jean-Luc Maloisel , Nicolas Thevenin
CPC分类号： B01J20/3253 , B01D15/3847 , B01J20/3242 , B01J20/3246 , B01J20/3255 , B01J20/3285
摘要： The present invention relates to a process for the purification of antibodies from one or more impurities in a liquid, which process comprises contacting said liquid with a first chromatography resin comprised of a support to which multi-modal ligands have been immobilised to adsorb the antibodies to the resin, wherein each multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system; adding an eluent to release the antibodies from the resin; and contacting the eluate so obtained with a second chromatography resin. In one embodiment, the ring-forming atoms of the aromatic or heteroaromatic entity are selected from the group consisting of C, S and O, and the cation exchanging group is a weak cation exchanger.
摘要翻译： 本发明涉及一种从液体中的一种或多种杂质纯化抗体的方法，该方法包括使所述液体与包含多元配体的载体的第一层析树脂接触以将抗体吸附到 所述树脂，其中每个多模式配体包含至少一个阳离子交换基团和至少一个芳族或杂芳族环系统; 添加洗脱液以从树脂中释放抗体; 并将如此获得的洗脱液与第二层析树脂接触。 在一个实施方案中，芳族或杂芳族实体的成环原子选自C，S和O，阳离子交换基团是弱阳离子交换剂。
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公开(公告)号：US07008542B2
公开(公告)日：2006-03-07
申请号：US10451192
申请日：2001-12-17
IPC分类号： B01D15/00
摘要： Method for the removal of a substance from an aqueous liquid by ion exchange comprising providing a liquid with said substance is present; providing an adsorption matrix which comprises at least two different ligands; contacting the liquid with the matrix under a period of time and conditions sufficient to allow adsorption of the substance to the matrix; and adding an eluent that desorbs the substance from the matrix. Each one of the ligands interacts with the substance during the adsorption step, and at least one of the ligands is charged and capable of ionic interaction with the substance. The method can be run as a cation or anion exchange. The substance desorption can be performed by adding an eluent comprising an increasing ionic strength. The invention also encompasses an adsorbent comprising at least two structurally different ligands, which interact with the same kind of substance when used in a separation procedure.
摘要翻译： 存在通过离子交换从含水液体中除去物质的方法，包括提供液体与所述物质的方法; 提供包含至少两种不同配体的吸附基质; 在足以允许物质吸附到基质的时间和条件下将液体与基质接触; 并加入从基质中解吸物质的洗脱液。 每个配体在吸附步骤期间与物质相互作用，并且至少一个配体被带电并且能够与该物质进行离子相互作用。 该方法可以作为阳离子或阴离子交换进行。 物质解吸可以通过加入含增加离子强度的洗脱液进行。 本发明还包括一种吸附剂，其包含至少两个结构不同的配体，当在分离过程中使用时，其与相同种类的物质相互作用。
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公开(公告)号：US20100009867A1
公开(公告)日：2010-01-14
申请号：US12517570
申请日：2008-01-10
CPC分类号： C40B30/04 , B01D15/1885 , B01D15/361 , B01D15/362 , B01D15/363 , B01D15/3847 , B01J20/3285 , B01J39/26 , B01J41/20 , B01J2220/54 , C40B40/14 , C40B50/00 , G01N30/466 , G01N30/96
摘要： The present invention relates to a method of preparing a library of resins which are useful in chromatography, which method comprises creating a diversity of multi-modal ion exchange resins; and providing the diversity in a parallel system in which each resin is presented separated from the other resin(s).
摘要翻译： 本发明涉及一种制备可用于色谱法的树脂文件库的方法，该方法包括产生多种多模式离子交换树脂; 并且在与其它树脂分离出每种树脂的并行系统中提供多样性。
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公开(公告)号：US07214321B2
公开(公告)日：2007-05-08
申请号：US10312054
申请日：2001-07-16
IPC分类号： B01D15/04
CPC分类号： B01J20/3285 , B01J39/04 , B01J39/18
摘要： The invention relates to a method for removing a positively charged substance from an aqueous liquid (I) by contacting the liquid with a cation-exchanger (1), possibly followed by a subsequent desorption of said substance. The cation-exchanger is selected to be capable of (a) binding to said substance by cation-exchange in an aqueous liquid reference (II) at an ionic strength corresponding to 0.3 M NaCl and (b) permitting a break through capacity for said substance 3200%, such as 3300% or 3500%, of the break-through capacity of said substance for a reference cation-exchanger (2) containing sulphopropyl groups —CH2CH2CH2SO2O—. The cation exchange ligands have an at least bimodal function by comprising a cation exchanging group and a separate hydrogen-bonding atom. The invention also relates to a method for testing the appropriateness of a cation-exchanger for removing a substance from a liquid and novel cation exchangers.
摘要翻译： 本发明涉及通过使液体与阳离子交换剂（1）接触，从而可以随后随后解吸所述物质从水性液体（I）中除去带正电荷的物质的方法。 阳离子交换剂被选择为能够（a）在水性液体参考物（II）中以对应于0.3M NaCl的离子强度的阳离子交换结合所述物质，和（b）允许所述物质的破裂能力 例如300％或3％以上500％以上的所述物质用于参考阳离子交换器的穿透能力的200％ （2）含有磺基丙基-CH 2 CH 2 CH 2 SO 2 O-。 阳离子交换配体通过包含阳离子交换基团和单独的氢键原子具有至少双峰功能。 本发明还涉及一种用于测试用于从液体和新型阳离子交换剂中除去物质的阳离子交换器的适当性的方法。
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公开(公告)号：US09266041B2
公开(公告)日：2016-02-23
申请号：US13158780
申请日：2011-06-13
申请人： Bo-Lennart Johansson , Jean-Luc Maloisel , Hans J. Johansson , Asa Ek , Maria Holm , Jafar Saidzadeh
发明人： Bo-Lennart Johansson , Jean-Luc Maloisel , Hans J. Johansson , Asa Ek , Maria Holm , Jafar Saidzadeh
IPC分类号： C07K1/14 , B01D15/32 , B01D15/36 , B01D15/38 , B01J20/28 , B01J20/286 , B01J20/32 , B01J41/20 , C07K1/16 , C07K1/18 , C07K1/36 , C07K16/00 , C07K16/06
CPC分类号： B01D15/327 , B01D15/363 , B01D15/3804 , B01J20/28033 , B01J20/286 , B01J20/3219 , B01J20/3253 , B01J20/3285 , B01J41/20 , C07K1/16 , C07K1/18 , C07K1/36 , C07K16/00 , C07K16/065 , Y10T29/49826
摘要： The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.
摘要翻译： 本发明涉及由下式R1-R2-N（R3）-R4-R5定义的色谱配体，其中R1是取代或未取代的苯基; R2是包含0-4个碳原子的烃链; R3是包含1-3个碳原子的烃链; R4是包含1-5个碳原子的烃链; 并且R 5是OH或H.本发明还包括分离基质，其包含与多孔载体如颗粒或膜耦合的所述配体。 根据本发明的配体和基质可用于纯化生物分子或有机化合物，例如蛋白质，多肽，DNA等。根据本发明的有利用途是抗体的纯化。
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