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公开(公告)号：US5719028A
公开(公告)日：1998-02-17
申请号：US789079
申请日：1997-02-06
IPC分类号： C07H21/00 , C07K14/47 , C07K14/82 , C12N9/12 , C12N15/10 , C12N15/113 , C12Q1/68 , C12Q1/70 , C12Q1/44
CPC分类号： C12N15/113 , C07H21/00 , C07K14/4746 , C07K14/82 , C12N15/10 , C12N9/1252 , C12Q1/6823 , C12Q1/6827
摘要： A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.
摘要翻译： 公开了以位点特异性方式切割核酸切割结构的方法。 采用具有5'核酸酶活性而不干扰核酸合成能力的切割酶作为检测特异性核酸序列的新方法的基础。
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22.发明授权
公开(公告)号：US5541311A
公开(公告)日：1996-07-30
申请号：US73384
申请日：1993-06-04
IPC分类号： C12N1/21 , C07H21/00 , C07K14/47 , C12N9/12 , C12N9/14 , C12N9/16 , C12N15/09 , C12N15/10 , C12N15/113 , C12N15/55 , C12Q1/44 , C12Q1/68 , C12Q1/70 , C12R1/19 , G01N33/53 , C07H21/04 , C12N15/00
CPC分类号： C12N9/14 , C07H21/00 , C07K14/4746 , C12N15/10 , C12N15/113 , C12N9/1252 , C12Q1/682 , C12Q1/6823 , C12Q1/6827 , C12Q1/6844 , C12Q1/6853 , C12Q1/686 , C12Q1/705 , C12Q2600/156 , Y10T436/143333
摘要： A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences. Cleaving enzymes are produced through the use of novel DNA sequences which encode novel thermostable polymerases.
摘要翻译： 公开了以位点特异性方式切割核酸切割结构的方法。 采用具有5'核酸酶活性而不干扰核酸合成能力的切割酶作为检测特异性核酸序列的新方法的基础。 通过使用编码新型热稳定聚合酶的新型DNA序列产生切割酶。
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公开(公告)号：US20090117576A1
公开(公告)日：2009-05-07
申请号：US12242508
申请日：2008-09-30
申请人： Fang Dong , Victor I. Lyamichev , James R. Prudent , Lance Fors , Bruce P. Neri , Mary Ann D. Brow , Todd A. Anderson , James E. Dahlberg
发明人： Fang Dong , Victor I. Lyamichev , James R. Prudent , Lance Fors , Bruce P. Neri , Mary Ann D. Brow , Todd A. Anderson , James E. Dahlberg
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C12Q1/701 , C12Q2565/133 , C12Q2521/301
摘要： The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
摘要翻译： 本发明涉及用于分析核酸的方法和组合物。 特别地，本发明提供了用于检测和表征核酸序列和序列变化的方法和组合物。 本发明的方法允许检测和/或鉴定遗传多态性，例如与人类疾病相关的遗传多态性，并允许鉴定病原体（例如病毒和细菌菌株鉴定）。
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公开(公告)号：US20080199936A1
公开(公告)日：2008-08-21
申请号：US11926006
申请日：2007-10-28
IPC分类号： C12N9/00
CPC分类号： C12N9/1252 , A61K38/00 , C07K2319/02 , C12N9/22 , C12Q1/6823 , C12Q2561/109
摘要： The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明提供了用于切割和修饰核酸的新型切割剂和聚合酶。 裂解剂和聚合酶可用于例如核酸序列的检测和表征以及核酸序列的变化。 在一些实施方案中，使用各种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US07381530B2
公开(公告)日：2008-06-03
申请号：US10897793
申请日：2004-07-23
申请人： Jeff G. Hall , Victor I. Lyamichev , Andrea L. Mast , Mary Ann D. Brow , Robert W. Kwiatkowski , Stephanie H. Vavra
发明人： Jeff G. Hall , Victor I. Lyamichev , Andrea L. Mast , Mary Ann D. Brow , Robert W. Kwiatkowski , Stephanie H. Vavra
IPC分类号： C12Q1/68
CPC分类号： C12N9/1252 , C12N9/22 , C12Q1/6823 , C12Q1/683 , C12Q1/701 , C12Q1/703 , C12Q1/706 , Y10S435/81 , C12Q2561/109
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。 本发明还涉及基于电荷分离核酸分子的方法和装置。 本发明还提供了通过形成完整和活化的蛋白质结合区域来检测非靶切割产物的方法。 本发明还提供用于检测样品中各种病毒的核酸的敏感和具体方法。
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公开(公告)号：US07101672B2
公开(公告)日：2006-09-05
申请号：US10655362
申请日：2003-09-04
申请人： Fang Dong , Victor I. Lyamichev , James R. Prudent , Lance Fors , Bruce P. Neri , Mary Ann D. Brow , Todd A. Anderson , James E. Dahlberg
发明人： Fang Dong , Victor I. Lyamichev , James R. Prudent , Lance Fors , Bruce P. Neri , Mary Ann D. Brow , Todd A. Anderson , James E. Dahlberg
CPC分类号： C12Q1/6832 , C12Q1/701 , C12Q2565/133 , C12Q2521/301
摘要： The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acids and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
摘要翻译： 本发明涉及用于分析核酸的方法和组合物。 特别地，本发明提供了用于检测和表征核酸和序列变化的方法和组合物。 本发明的方法允许检测和/或鉴定遗传多态性，例如与人类疾病相关的遗传多态性，并允许鉴定病原体（例如病毒和细菌菌株鉴定）。
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公开(公告)号：US06875572B2
公开(公告)日：2005-04-05
申请号：US10081806
申请日：2002-02-22
CPC分类号： C12Q1/6823 , C12N9/22 , C12Q1/6827 , C12Q1/683 , C12Q2563/149 , C12Q2561/109
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US06673616B1
公开(公告)日：2004-01-06
申请号：US09655378
申请日：2000-09-05
IPC分类号： C12Q168
CPC分类号： C12N15/113 , C07H21/00 , C07K14/4746 , C07K14/82 , C12N9/1252 , C12N15/10 , C12Q1/68 , C12Q1/682 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , C12Q1/6853 , C12Q1/686 , C12Q1/689 , C12Q1/705 , Y10T436/143333 , C12Q2537/149 , C12Q2525/301 , C12Q2521/319
摘要： The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.
摘要翻译： 本发明涉及以位点特异性方式切割核酸切割结构的方法。 酶包括5'核酸酶和3'核酸外切酶，用于检测和鉴定衍生自微生物的核酸。 提供了允许在样品中检测和鉴定细菌和病毒病原体的方法。
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公开(公告)号：US06214545B1
公开(公告)日：2001-04-10
申请号：US08851588
申请日：1997-05-05
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q2565/133 , C12Q2565/501
摘要： The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
摘要翻译： 本发明涉及用于分析核酸的方法和组合物。 特别地，本发明提供了用于检测和表征核酸序列和序列变化的方法和组合物。 本发明的方法允许检测和/或鉴定遗传多态性，例如与人类疾病相关的遗传多态性，并允许鉴定病原体（例如病毒和细菌菌株鉴定）。
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30.发明授权Polymorphism analysis by nucleic acid structure probing with structure-bridging oligonucleotides 失效通过结构桥接寡核苷酸的核酸结构探测进行多态性分析公开(公告)号：US06210880B1
公开(公告)日：2001-04-03
申请号：US08934097
申请日：1997-09-19
申请人： Victor I. Lyamichev , Fang Dong , Mary Ann D. Brow , Lance Fors , Bruce P. Neri
发明人： Victor I. Lyamichev , Fang Dong , Mary Ann D. Brow , Lance Fors , Bruce P. Neri
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q2565/133
摘要： The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
摘要翻译： 本发明涉及用于分析核酸的方法和组合物。 特别地，本发明提供了用于检测和表征核酸序列和序列变化的方法和组合物。 本发明的方法允许检测和/或鉴定遗传多态性，例如与人类疾病相关的遗传多态性，并允许鉴定病原体（例如病毒和细菌菌株鉴定）。
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