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公开(公告)号：US20060003414A1
公开(公告)日：2006-01-05
申请号：US11152811
申请日：2005-06-14
IPC分类号： C12P21/06 , C07H21/04 , C12N9/02 , C12N9/10 , C12N9/16 , C12N9/20 , C12N1/16 , C12N9/24 , C12N15/74
CPC分类号： C12N9/6472 , C07K14/37 , C12N9/16 , C12N9/2437 , C12N9/6424 , C12N15/625
摘要： The present invention relates to a method for producing a polypeptide comprising using a signal peptide, to nucleic acid constructs comprising a first nucleotide sequence encoding the signal peptide and a second nucleotide sequence encoding a polypeptide which is foreign to the first nucleotide sequence. Furthermore, it also relates to expression vectors and host cells comprising the nuclei acid construct.
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公开(公告)号：US20050031312A1
公开(公告)日：2005-02-10
申请号：US10895377
申请日：2004-07-21
申请人： Tomoko Matsui , Ryouji Yamaguchi , Tadashi Shibata
发明人： Tomoko Matsui , Ryouji Yamaguchi , Tadashi Shibata
CPC分类号： H04N9/8063 , G11B20/10 , G11B2020/1062 , G11B2020/10694 , G11B2020/10814 , H04N5/781 , H04N5/85 , H04N5/907 , H04N9/8042 , H04N9/8047
摘要： This invention provides a method and apparatus for signal reproduction that can simultaneously reproduce N (N≧2) signals which are previously multiplexed, and simultaneously reproduce N independent signals. This apparatus selectively inputs the independent N coded signals in time division, adds the corresponding stream information to the selected input signal among N kinds of stream information for identifying the input signals, detects the stream information from the respective input signal, decodes the respective input signal by a decoding method corresponding to the respective input signal, which is decided based on the detected stream information, and outputs the decoded signal.
摘要翻译： 本发明提供一种用于信号再现的方法和装置，其可以同时再现先前多路复用的N（N> = 2）个信号，同时再现N个独立信号。 该装置以时分方式选择性地输入独立的N个编码信号，将N个用于识别输入信号的流信息中的所选输入信号相加的流信息相加，从相应的输入信号检测流信息，解码各输入信号 通过与基于检测到的流信息决定的各个输入信号对应的解码方式，输出解码信号。
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公开(公告)号：US09534239B2
公开(公告)日：2017-01-03
申请号：US14343278
申请日：2012-09-05
申请人： Tomoko Matsui , Suzanne Clark
发明人： Tomoko Matsui , Suzanne Clark
CPC分类号： C12P7/14 , C12C5/004 , C12N9/2428 , C12P19/02 , C12P19/14 , C12Y302/01003 , C13K1/06 , Y02P20/52
摘要： The present invention relates to glucoamylase variants having reduced sensitivity to protease nicking. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
摘要翻译： 本发明涉及对蛋白酶切口具有降低的敏感性的葡糖淀粉酶变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。
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公开(公告)号：US20120225468A1
公开(公告)日：2012-09-06
申请号：US13472822
申请日：2012-05-16
申请人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
发明人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
IPC分类号： C12N9/16
CPC分类号： C12N9/16 , A23K20/189 , A23K50/30 , A23K50/75 , Y02E50/17
摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.
摘要翻译： 本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。 这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度 简介 和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。
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公开(公告)号：US08206962B2
公开(公告)日：2012-06-26
申请号：US12568312
申请日：2009-09-28
申请人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
发明人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
CPC分类号： C12N9/16 , A23K20/189 , A23K50/30 , A23K50/75 , Y02E50/17
摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.
摘要翻译： 本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。 这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度 简介 和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。
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公开(公告)号：US08124379B2
公开(公告)日：2012-02-28
申请号：US12415174
申请日：2009-03-31
CPC分类号： C12N9/6472 , C07K14/37 , C12N9/16 , C12N9/2437 , C12N9/6424 , C12N15/625
摘要： The present invention relates to a method for producing a polypeptide comprising using a signal peptide, to nucleic acid constructs comprising a first nucleotide sequence encoding the signal peptide and a second nucleotide sequence encoding a polypeptide which is foreign to the first nucleotide sequence. Furthermore, it also relates to expression vectors and host cells comprising the nuclei acid construct.
摘要翻译： 本发明涉及包含使用信号肽的多肽的制备方法，其包含编码信号肽的第一核苷酸序列的核酸构建体和编码第一核苷酸序列异源的多肽的第二核苷酸序列。 此外，它还涉及包含核酸构建体的表达载体和宿主细胞。
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公开(公告)号：US20100180095A1
公开(公告)日：2010-07-15
申请号：US12095610
申请日：2006-11-28
IPC分类号： G06F12/14
CPC分类号： G06F5/14 , G06F2205/062
摘要： The buffer control device of this invention includes: a pointer holding unit which holds a virtual pointer different from a read pointer and a write pointer; an access control unit that controls an access to a ring buffer; a judging unit that judges whether or not one of the read pointer and the write pointer has reached an address substantially identical to an address indicated by the virtual pointer; and disabling unit that disables a normal access using the one of the read pointer and the write pointer, when the judging unit judges that the one of the read pointer and the write pointer has reached the address substantially identical to the address indicated by the virtual pointer, the normal access being controlled by the access control unit, wherein the access control unit further controls a reaccess to the ring buffer.
摘要翻译： 本发明的缓冲器控制装置包括：保持与读指针不同的虚拟指针和写指针的指针保持单元; 访问控制单元，其控制对环形缓冲器的访问; 判断单元，判断读指针和写指针之一是否达到与虚拟指针所指示的地址基本相同的地址; 以及禁止单元，其在所述读取指针和所述写入指针之一达到与所述虚拟指针所指示的地址基本相同的地址的情况下，使用所述读取指针和所述写入指针中的一者禁止正常访问 正常访问由访问控制单元控制，其中访问控制单元进一步控制对环形缓冲器的重新访问。
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公开(公告)号：US07666652B2
公开(公告)日：2010-02-23
申请号：US12044055
申请日：2008-03-07
CPC分类号： C12N9/82 , A23L5/25 , A23L19/18 , C12Y305/01001
摘要： The invention relates to new asparaginases having improved properties, preferably improved thermotolerance, such as improved activity at high temperatures and/or improved thermostability. The invention also relates to DNA sequences encoding such improved asparaginases, their production in a recombinant host cell, as well as methods of using the asparaginases, in particular for reduction of acrylamide in foods. The invention furthermore relates to methods of generating and preparing asparaginase variants having improved properties.
摘要翻译： 本发明涉及具有改进性能的新型天冬酰胺酶，优选改进的耐热性，例如在高温下改善的活性和/或改善的热稳定性。 本发明还涉及编码这种改良天冬酰胺酶的DNA序列，其在重组宿主细胞中的产生，以及使用天冬酰胺酶，特别是用于还原食品中丙烯酰胺的方法。 本发明还涉及产生和制备具有改进性质的天冬酰胺酶变体的方法。
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公开(公告)号：US20090170157A1
公开(公告)日：2009-07-02
申请号：US12044055
申请日：2008-03-07
CPC分类号： C12N9/82 , A23L5/25 , A23L19/18 , C12Y305/01001
摘要： The invention relates to new asparaginases having improved properties, preferably improved thermotolerance, such as improved activity at high temperatures and/or improved thermostability. The invention also relates to DNA sequences encoding such improved asparaginases, their production in a recombinant host cell, as well as methods of using the asparaginases, in particular for reduction of acrylamide in foods. The invention furthermore relates to methods of generating and preparing asparaginase variants having improved properties.
摘要翻译： 本发明涉及具有改进性能的新型天冬酰胺酶，优选改进的耐热性，例如在高温下改善的活性和/或改善的热稳定性。 本发明还涉及编码这种改良天冬酰胺酶的DNA序列，其在重组宿主细胞中的产生，以及使用天冬酰胺酶，特别是用于还原食品中丙烯酰胺的方法。 本发明还涉及产生和制备具有改进性质的天冬酰胺酶变体的方法。
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公开(公告)号：US09040280B2
公开(公告)日：2015-05-26
申请号：US13510076
申请日：2010-12-10
CPC分类号： C12N9/58 , C12P7/06 , C12Y304/24 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to proteases having at least 75% identity to a protease derived from Thermoascus aurantiacus and comprises at least one modification in the amino acid sequence thereof. These protease variants have improved thermostability. The invention also relates to DNA encoding these proteases, methods of their production, as well as the use thereof.
摘要翻译： 本发明涉及与衍生自嗜热嗜铬鱼的蛋白酶具有至少75％同一性的蛋白酶，并且包含其氨基酸序列中的至少一个修饰。 这些蛋白酶变体具有改善的热稳定性。 本发明还涉及编码这些蛋白酶的DNA，其生产方法及其用途。
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