公开(公告)号：US20230182102A1

公开(公告)日：2023-06-15

申请号：US18164341

申请日：2023-02-03

Applicant: ILLUMINA, INC.

Inventor： Tarun Kumar Khurana ,  Foad Mashayekhi ,  Hei Ka Patrick Tam

IPC: B01J13/14 ,  B01L3/00 ,  C12Q1/6806

CPC classification number: B01J13/14 ,  B01L3/502792 ,  C12Q1/6806 ,  B01L2200/0673 ,  B01L2300/047 ,  B01L2300/0858 ,  B01L2400/084 ,  B01L2400/0403

Abstract: A microfluidic droplet generator that includes a body, an inlet arranged adjacent an upper surface of the body, and a sample reservoir adapted to contain a reservoir fluid that is immiscible in water. The sample reservoir includes a floor and a sidewall coupled to the floor. The floor extends along a horizontal axis and the sidewall extends along a vertical axis substantially perpendicular to the horizontal axis. The microfluidic droplet generator also includes one or more microchannels fluidly connecting the inlet to the sample reservoir. Each of the microchannels includes an inlet end and a reservoir end, and the reservoir end of each of the microchannels intersects the sidewall of the sample reservoir at a location beneath the upper surface of the body.

公开(公告)号：US20230063675A1

公开(公告)日：2023-03-02

申请号：US17978130

申请日：2022-10-31

Applicant: ILLUMINA, INC.

Inventor： Xi-Jun Chen ,  Yir-Shyuan Wu ,  Tarun Kumar Khurana ,  Liangliang Qiang ,  Andrew J. Price ,  Elisabet Rosas

IPC: C12Q1/6874 ,  C12N15/10

Abstract: An example of a sequencing kit includes a flow cell and an encapsulation matrix precursor composition. The flow cell includes a plurality of chambers and primers attached within each of the plurality of chambers. The encapsulation matrix precursor composition consists of a fluid and a polymer selected from the group consisting of agar, agarose, alginate, heparin, alginate sulfate, dextran sulfate, hyaluronan, pectin, carrageenan, gelatin, chitosan, cellulose, a collagen polymer, and combinations thereof.

公开(公告)号：US20220143603A1

公开(公告)日：2022-05-12

申请号：US17435229

申请日：2020-11-25

Applicant: ILLUMINA, INC.

Inventor： Tarun Kumar Khurana ,  Elisabet ROSAS-CANYELLES ,  Yir-shyuan WU ,  Hayden Black ,  Mathieu LESSARD-VIGER ,  Max Zimmerly ,  Sean Ramirez

IPC: B01L3/00 ,  C08F2/48 ,  G03F7/00 ,  B01L7/00 ,  C40B30/04 ,  G03F7/028 ,  C08F220/56

Abstract: A method for making on-flow cell three-dimensional polymer structures includes loading a polymer precursor solution onto a flow cell. The polymer precursor solution includes a monomer, a crosslinker, and a photoinitiator. The flow cell includes at least one channel for receiving the polymer precursor solution. The at least one channel has an upper interior surface and a lower interior surface. The method further includes illuminating the polymer precursor solution through a patterned photomask using a light at a wavelength sufficient to activate the photoinitiator. Activation of the photoinitiator polymerizes at least some of the polymer precursor solution underneath apertures in the patterned photomask and forms three-dimensional polymer structures that extend from the upper interior surface to the lower interior surface of the at least one channel.

公开(公告)号：US11180752B2

公开(公告)日：2021-11-23

申请号：US16272870

申请日：2019-02-11

Applicant: Illumina, Inc.

Inventor： Yir-Shyuan Wu ,  Filiz Gorpe-Yasar ,  Tarun Kumar Khurana ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC: C12N15/10 ,  B01L3/00 ,  C12Q1/6834 ,  C12Q1/6806 ,  B01L7/00

Abstract: Systems, methods, and compositions provided herein relate to preparation of beads encapsulating long DNA fragments for high-throughput spatial indexing. Some embodiments include preparation of nucleic acid libraries within the bead, wherein the bead includes pores that allow diffusion of reagents while retaining genetic material.

公开(公告)号：US20210238589A1

公开(公告)日：2021-08-05

申请号：US17166906

申请日：2021-02-03

Applicant: ILLUMINA, INC.

Inventor： Dan Cao ,  Jeffrey S. Fisher ,  Fiona Kaper ,  Tarun Kumar Khurana ,  Tong Liu ,  Burak Okumus ,  Victor J. Quijano ,  Clifford Lee Wang ,  Yir-Shyuan Wu ,  Shi Min Xiao ,  Hongxia Xu

IPC: C12N15/10

Abstract: An example of a biotin-streptavidin cleavage composition includes a formamide reagent and a salt buffer. The formamide reagent is present in the biotin-streptavidin cleavage composition in an amount ranging from about 10% to about 50%, based on a total volume of the biotin-streptavidin cleavage composition. The salt buffer makes up the balance of the biotin-streptavidin cleavage composition. In some examples, the biotin-streptavidin cleavage composition is used to cleave library fragments from a solid support. In other examples, other mechanisms are used to cleave library fragments from a solid support.

公开(公告)号：US20200325467A1

公开(公告)日：2020-10-15

申请号：US16626221

申请日：2018-06-28

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Brian D. Mather ,  Cyril Delattre ,  Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Pallavi Daggumati ,  Behnam Javanmardi ,  Filiz Gorpe-Yasar ,  Sebastien Georg Gabriel Ricoult ,  Xavier von Hatten ,  Daniel Leonard Fuller

IPC: C12N15/10 ,  C08F226/10

Abstract: An example of a nucleic acid extraction material includes a substrate. The nucleic acid extraction material also includes a polycation bonded to at least a portion of a surface of the substrate. The polycation consists of a polymer of a quaternized monomer selected from the group consisting of a quaternized 1-vinylimidazole monomer and a quaternized dimethylaminoethyl methacrylate monomer, or a copolymer of a neutral monomer and the quaternized monomer.

公开(公告)号：US20200246771A1

公开(公告)日：2020-08-06

申请号：US16780104

申请日：2020-02-03

Applicant: Illumina, Inc.

Inventor： Tarun Kumar Khurana ,  Foad Mashayekhi ,  Hei Ka Patrick Tam

IPC: B01J13/14 ,  B01L3/00 ,  C12Q1/6806

Abstract: Disclosed herein is a novel method of producing monodisperse aqueous droplets, as well as a novel microfluidic droplet generator. In some examples, the method comprises flowing an aqueous solution through a microchannel and into a sample reservoir of the microfluidic droplet generator, wherein monodisperse droplets of the aqueous solution form by step-emulsification at a step change in height at an intersection of a reservoir end of the microchannel and a sidewall of the sample reservoir. In some examples, the aqueous solution is a hydrogel precursor solution and monodisperse droplets of the hydrogel precursor solution form by step-emulsification at the step change in height at the intersection of the reservoir end of the microchannel and the sidewall of the sample reservoir. In some examples, the monodisperse droplets of the hydrogel precursor solution are incubated under conditions suitable for gelation to form hydrogel beads.

公开(公告)号：US20200239954A1

公开(公告)日：2020-07-30

申请号：US16750897

申请日：2020-01-23

Applicant: ILLUMINA, INC.

Inventor： Xi-Jun Chen ,  Yir-Shyuan Wu ,  Tarun Kumar Khurana ,  Liangliang Qiang ,  Andrew J. Price ,  Elisabet Rosas

IPC: C12Q1/6874 ,  C12N15/10

Abstract: An example of a sequencing kit includes a flow cell, an encapsulation matrix precursor composition, and a radical initiator. The flow cell includes a plurality of chambers and primers attached within each of the plurality of chambers. The encapsulation matrix precursor composition consists of a fluid, a monomer or polymer including a radical generating and chain elongating functional group, a radical source, and a crosslinker. The radical initiator is part of the encapsulation matrix precursor composition or is a separate component.

公开(公告)号：US20190249171A1

公开(公告)日：2019-08-15

申请号：US16272870

申请日：2019-02-11

Applicant: Illumina, Inc.

Inventor： Yir-Shyuan Wu ,  Filiz Gorpe-Yasar ,  Tarun Kumar Khurana ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC: C12N15/10 ,  C12Q1/6806 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12N15/1068 ,  B01L3/508 ,  B01L7/52 ,  B01L2200/141 ,  B01L2200/16 ,  B01L2300/123 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2527/156 ,  C12Q2531/119 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: Systems, methods, and compositions provided herein relate to preparation of beads encapsulating long DNA fragments for high-throughput spatial indexing. Some embodiments include preparation of nucleic acid libraries within the bead, wherein the bead includes pores that allow diffusion of reagents while retaining genetic material.

公开(公告)号：US12172156B2

公开(公告)日：2024-12-24

申请号：US18233289

申请日：2023-08-11

Applicant: ILLUMINA, INC.

Inventor： Lewis J. Kraft ,  Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Arnaud Rival ,  Justin Fullerton ,  M. Shane Bowen ,  Hui Han ,  Jeffrey S. Fisher ,  Yasaman Farshchi ,  Mathieu Lessard-Viger

IPC: B01L3/00 ,  B01J19/00 ,  C12N15/10 ,  C40B40/08 ,  C40B50/18 ,  C08L37/00 ,  G01N21/05

Abstract: An example of a flow cell includes a substrate, a plurality of chambers defined on or in the substrate, and a plurality of depressions defined in the substrate and within a perimeter of each of the plurality of chambers. The depressions are separated by interstitial regions. Primers are attached within each of the plurality of depressions, and a capture site is located within each of the plurality of chambers.