公开(公告)号：US20140131203A1

公开(公告)日：2014-05-15

申请号：US13738315

申请日：2013-01-10

Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION

Inventor： Hongbo Peng ,  Gustavo A. Stolovitzky ,  Deqiang Wang

IPC: G01N33/487

CPC classification number: G01N33/48721 ,  B82Y30/00 ,  C12Q1/6869 ,  C12Q2565/631

Abstract: A mechanism is provided for capturing a molecule via an integrated system. An alternating voltage is applied to a Paul trap device in an electrically conductive solution to generate electric fields. The Paul trap device is integrated with a nanopore device to form the integrated system. Forces from the electric fields of the Paul trap device position the molecule to a nanopore in the nanopore device. A first voltage is applied to the nanopore device to capture the molecule in the nanopore of the nanopore device.

公开(公告)号：US20200016596A1

公开(公告)日：2020-01-16

申请号：US16581837

申请日：2019-09-25

Applicant: International Business Machines Corporation

Inventor： Huan Hu ,  Joshua T. Smith ,  Gustavo A. Stolovitzky ,  Benjamin H. Wunsch

IPC: B01L3/00 ,  H01L21/3105 ,  H01L21/3213 ,  H01L29/06

Abstract: Techniques relate to forming a sorting device. A mesh is formed on top of a substrate. Metal assisted chemical etching is performed to remove substrate material of the substrate at locations of the mesh. Pillars are formed in the substrate by removal of the substrate material. The mesh is removed to leave the pillars in a nanopillar array. The pillars in the nanopillar array are designed with a spacing to sort particles of different sizes such that the particles at or above a predetermined dimension are sorted in a first direction and the particles below the predetermined dimension are sorted in a second direction.

公开(公告)号：US10386276B2

公开(公告)日：2019-08-20

申请号：US15270478

申请日：2016-09-20

Applicant: International Business Machines Corporation

Inventor： Stacey M. Gifford ,  Gustavo A. Stolovitzky ,  Benjamin H. Wunsch

IPC: B01D15/00 ,  G01N1/34 ,  B01L3/00 ,  G01N1/40 ,  B01D15/34 ,  G01N30/00 ,  G01N33/537

Abstract: Techniques for phosphoprotein detection, quantification, and purification using a chip-based pillar array are provided. In one aspect, a method for purifying a protein sample includes: introducing a mixture including the protein sample and an antibody to a nanoDLD array having a plurality of pillars separated by a gap g, wherein the antibody and proteins in the protein sample form antibody-protein complexes having a size that is greater than a size threshold of the nanoDLD array created by the gap g which permits size-based separation of the antibody-protein complexes as the mixture flows through the nanoDLD array; and collecting a purified protein sample containing the antibody-protein complexes from the nanoDLD array. A lab-on-a-chip (LOC) device including the nanoDLD array is also provided.

公开(公告)号：US10385390B2

公开(公告)日：2019-08-20

申请号：US15637038

申请日：2017-06-29

Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION

Inventor： Hongbo Peng ,  Gustavo A. Stolovitzky ,  Wenjuan Zhu

IPC: G01N27/62 ,  C12Q1/68 ,  G01N33/487 ,  C12Q1/6869 ,  B82Y5/00

Abstract: A technique for a nanodevice is provided. A reservoir is separated into two parts by a membrane. A nanopore is formed through the membrane, and the nanopore connects the two parts of the reservoir. The nanopore and the two parts of the reservoir are filled with ionic buffer. The membrane includes a graphene layer and insulating layers. The graphene layer is wired to first and second metal pads to form a graphene transistor in which transistor current flowing through the graphene transistor is modulated by charges or dipoles passing through the nanopore.

公开(公告)号：US20180196924A1

公开(公告)日：2018-07-12

申请号：US15401115

申请日：2017-01-09

Applicant: International Business Machines Corporation

Inventor： Solomon Assefa ,  Geoffrey H. Siwo ,  Gustavo A. Stolovitzky

IPC: G06F19/00 ,  G06N7/00 ,  A61B5/00

CPC classification number: G16H50/20 ,  A61B5/0022 ,  A61B5/02 ,  A61B5/7246 ,  A61B5/7275 ,  A61B5/7282 ,  G16H50/30

Abstract: A computer-implemented method of diagnosis of a patient comprises comparing a marker-print of a patient, wherein the marker-print comprises an N-value vector with each value in the vector indicative of a state of a biological marker of the patient, against a compendium of reference marker-prints, each reference marker-print having an associated biological condition, the reference marker-prints being stored in a marker-print database, to determine at least one reference marker-print having at least one matching value with the patient marker print. The method may comprise calculating, by a confidence module of the computer processor, a level of similarity between the patient marker-print and the at least one determined reference marker-print with the at least one matching value, thereby to provide an indication of a confidence level that the patient has the biological condition associated with the at least one determined reference marker-print having the at least one matching value.

公开(公告)号：US09975147B2

公开(公告)日：2018-05-22

申请号：US14749309

申请日：2015-06-24

Applicant: International Business Machines Corporation

Inventor： Yann A. Astier ,  Joshua T. Smith ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Benjamin H. Wunsch

IPC: B07B1/00 ,  B07B13/04 ,  B07B1/46 ,  G01N15/00 ,  B82Y30/00 ,  G01N15/02

CPC classification number: B07B13/04 ,  B07B1/4609 ,  B82Y30/00 ,  G01N15/02 ,  G01N2015/0065 ,  G01N2015/0288 ,  Y10S977/84

Abstract: A technique relates sorting entities. The entities are introduced into a nanopillar array. The entities include a first population and a second population, and the nanopillar array includes nanopillars arranged to have a gap separating one from another. The nanopillars are ordered to have an array angle relative to a fluid flow direction. The entities are sorted through the nanopillar array by transporting the first population of the entities less than a predetermined size in a first direction and by transporting the second population of the entities at least the predetermined size in a second direction different from the first direction. The nanopillar array is configured to employ the gap with a gap size less than 300 nanometers in order to sort the entities having a sub-100 nanometer size.

公开(公告)号：US20170298429A1

公开(公告)日：2017-10-19

申请号：US15637038

申请日：2017-06-29

Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION

Inventor： Hongbo Peng ,  Gustavo A. Stolovitzky ,  Wenjuan Zhu

IPC: C12Q1/68 ,  G01N33/487 ,  B82Y5/00

CPC classification number: C12Q1/6869 ,  B82Y5/00 ,  G01N33/48721 ,  Y10T436/143333

Abstract: A technique for a nanodevice is provided. A reservoir is separated into two parts by a membrane. A nanopore is formed through the membrane, and the nanopore connects the two parts of the reservoir. The nanopore and the two parts of the reservoir are filled with ionic buffer. The membrane includes a graphene layer and insulating layers. The graphene layer is wired to first and second metal pads to form a graphene transistor in which transistor current flowing through the graphene transistor is modulated by charges or dipoles passing through the nanopore.

公开(公告)号：US09758822B2

公开(公告)日：2017-09-12

申请号：US14163141

申请日：2014-01-24

Applicant: International Business Machines Corporation

Inventor： Hongbo Peng ,  Gustavo A. Stolovitzky ,  Wenjuan Zhu

IPC: G01N27/62 ,  C12Q1/68 ,  G01N33/487 ,  B82Y5/00

CPC classification number: C12Q1/6869 ,  B82Y5/00 ,  G01N33/48721 ,  Y10T436/143333

Abstract: A technique for a nanodevice is provided. A reservoir is separated into two parts by a membrane. A nanopore is formed through the membrane, and the nanopore connects the two parts of the reservoir. The nanopore and the two parts of the reservoir are filled with ionic buffer. The membrane includes a graphene layer and insulating layers. The graphene layer is wired to first and second metal pads to form a graphene transistor in which transistor current flowing through the graphene transistor is modulated by charges or dipoles passing through the nanopore.

公开(公告)号：US09513277B2

公开(公告)日：2016-12-06

申请号：US15005174

申请日：2016-01-25

Applicant: International Business Machines Corporation

Inventor： Ali Afzali-Ardakani ,  Stefan Harrer ,  Binquan Luan ,  Hongbo Peng ,  Stephen M. Rossnagel ,  Ajay K. Royyuru ,  Gustavo A. Stolovitzky ,  Philip S. Waggoner

IPC: G01N33/487 ,  B82Y15/00 ,  C12Q1/68 ,  G01N27/447

CPC classification number: G01N33/48721 ,  B82Y15/00 ,  C12Q1/6869 ,  G01N27/44743 ,  G01N27/44791 ,  Y10S977/924 ,  C12Q2563/157

Abstract: A nanodevice includes a reservoir filled with conductive fluid and a membrane separating the reservoir. A nanopore is formed through the membrane having electrode layers separated by insulating layers. A certain electrode layer has a first type of organic coating and a pair of electrode layers has a second type. The first type of organic coating forms a motion control transient bond to a molecule in the nanopore for motion control, and the second type forms first and second transient bonds to different bonding sites of a base of the molecule. When a voltage is applied to the pair of electrode layers a tunneling current is generated by the base in the nanopore, and the tunneling current travels via the first and second transient bonds formed to be measured as a current signature for distinguishing the base. The motion control transient bond is stronger than first and second transient bonds.

公开(公告)号：US20160144405A1

公开(公告)日：2016-05-26

申请号：US14697072

申请日：2015-04-27

Applicant: International Business Machines Corporation

Inventor： Yann A. Astier ,  Joshua T. Smith ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Benjamin H. Wunsch

IPC: B07B13/04 ,  G01N15/10

CPC classification number: B07B13/04 ,  B07B1/4609 ,  B82Y30/00 ,  G01N15/02 ,  G01N2015/0065 ,  G01N2015/0288 ,  Y10S977/84

Abstract: A technique relates sorting entities. The entities are introduced into a nanopillar array. The entities include a first population and a second population, and the nanopillar array includes nanopillars arranged to have a gap separating one from another. The nanopillars are ordered to have an array angle relative to a fluid flow direction. The entities are sorted through the nanopillar array by transporting the first population of the entities less than a predetermined size in a first direction and by transporting the second population of the entities at least the predetermined size in a second direction different from the first direction. The nanopillar array is configured to employ the gap with a gap size less than 300 nanometers in order to sort the entities having a sub-100 nanometer size.