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公开(公告)号：US20180080857A1
公开(公告)日：2018-03-22
申请号：US15270478
申请日：2016-09-20
IPC分类号： G01N1/34 , G01N33/543
CPC分类号： G01N1/34 , B01D15/34 , B01L3/50273 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2200/0652 , B01L2400/0415 , G01N1/40 , G01N30/0005 , G01N33/537
摘要： Techniques for phosphoprotein detection, quantification, and purification using a chip-based pillar array are provided. In one aspect, a method for purifying a protein sample includes: introducing a mixture including the protein sample and an antibody to a nanoDLD array having a plurality of pillars separated by a gap g, wherein the antibody and proteins in the protein sample form antibody-protein complexes having a size that is greater than a size threshold of the nanoDLD array created by the gap g which permits size-based separation of the antibody-protein complexes as the mixture flows through the nanoDLD array; and collecting a purified protein sample containing the antibody-protein complexes from the nanoDLD array. A lab-on-a-chip (LOC) device including the nanoDLD array is also provided.
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公开(公告)号：US20170234833A1
公开(公告)日：2017-08-17
申请号：US15583501
申请日：2017-05-01
发明人： Binquan Luan , Gustavo A. Stolovitzky , Chao Wang , Deqiang Wang
IPC分类号： G01N27/447 , C12Q1/68 , G01N33/487
CPC分类号： G01N27/44791 , B82Y30/00 , C12Q1/6869 , G01N27/403 , G01N33/48721
摘要： Techniques for increasing the capture zone in nano and microchannel-based polymer testing structures using concentric arrangements of nanostructures, such as nanopillars are provided. In one aspect, a testing structure for testing polymers is provided that includes a first fluid reservoir and a second fluid reservoir formed in an electrically insulating substrate; at least one channel formed in the insulating substrate that interconnects the first fluid reservoir and the second fluid reservoir; and an arrangement of nanostructures within either the first fluid reservoir or the second fluid reservoir wherein the nanostructures are arranged so as to form multiple concentric circles inside either the first fluid reservoir or the second fluid reservoir with each of the concentric circles being centered at an entry point of the channel. A method of analyzing a polymer using the testing structure is also provided.
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公开(公告)号：US20170212098A1
公开(公告)日：2017-07-27
申请号：US15005576
申请日：2016-01-25
发明人： Gustavo A. Stolovitzky , Chao Wang
IPC分类号： G01N33/487 , B01L3/00
CPC分类号： G01N33/48721 , B01L3/502715 , B01L3/502761 , B01L2200/0663 , B01L2300/0816 , B01L2300/0896 , B01L2400/086
摘要： A technique relates to stretching an extensible molecule. The molecule moves through an array of pillars in a flow direction, where the array has an interface connecting a first pillar region and a second pillar region. Stretching the molecule by traversing the molecule in the flow direction through the interface connecting the first pillar region to the second pillar region, such that a first end and a second end of the molecule straddle a straddle pillar, thereby causing the first end to extend along a first path in the second and the second end to extend along a second path. Traversing the molecule stretches the first end and the second end along two different paths. The molecule is further traversed through the array such that the second end follows the first end along the first path, where the stretching causes the molecule to be in an uncoiled state.
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4.发明申请公开(公告)号：US20160199833A1
公开(公告)日：2016-07-14
申请号：US15074701
申请日：2016-03-18
发明人： Qinghuang Lin , Gustavo A. Stolovitzky , Chao Wang , Deqiang Wang
IPC分类号： B01L3/00
CPC分类号： G01N33/48721 , B01L3/502707 , B01L3/502715 , B01L3/502761 , B01L2200/0663 , B01L2200/12 , B01L2300/042 , B01L2300/0645 , B01L2300/0887 , B01L2300/0896 , B01L2300/161 , B01L2400/0415 , B01L2400/0421 , G01N27/447 , G01N27/44791 , Y10T29/49002
摘要： A device for passing a biopolymer molecule includes a nanochannel formed between a surface relief structure, a patterned layer forming sidewalls of the nanochannel and a sealing layer formed over the patterned layer to encapsulate the nanochannel. The surface relief structure includes a three-dimensionally rounded surface that reduces a channel dimension of the nanochannel at a portion of nanochannel and gradually increases the dimension along the nanochannel toward an opening position, which is configured to receive a biopolymer.
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公开(公告)号：US20160146718A1
公开(公告)日：2016-05-26
申请号：US14749202
申请日：2015-06-24
IPC分类号： G01N15/10
CPC分类号： G01N15/1031 , B01L3/502707 , B01L3/502761 , B01L2200/0652 , B01L2300/0864 , B01L2300/161 , G01N15/02 , G01N27/3278 , G01N33/48721 , G01N2015/0038 , G01N2015/0288
摘要： A technique relates sorting biopolymers. The biopolymers are introduced into a nanopillar array, and the biopolymers include a first population and a second population. The nanopillar array includes nanopillars arranged to have a gap separating one from another. The biopolymers are sorted through the nanopillar array by transporting the first population of the biopolymers less than a predetermined bumping size according to a fluid flow direction and by transporting the second population of the biopolymers at least the predetermined bumping size according to a bumped direction different from the fluid flow direction. The nanopillar array is configured to employ the gap with a gap size less than 300 nanometers in order to sort the biopolymers.
摘要翻译： 一种技术涉及排序生物聚合物。 将生物聚合物引入纳米柱阵列中，并且生物聚合物包括第一群体和第二群体。 纳米柱阵列包括布置成具有彼此分离的间隙的纳米柱。 生物聚合物通过纳米柱阵列分选，根据流体流动方向传送生物聚合物的第一群体小于预定的碰撞尺寸，并且根据不同于第一群体的碰撞方向，将生物聚合物的第二群体输送至少至少预定的碰撞大小 流体流动方向。 纳米柱阵列被配置为采用具有小于300纳米的间隙尺寸的间隙，以分类生物聚合物。
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6.发明授权Integrated nanopore and paul trap mechanism for DNA capture and motion control 有权用于DNA捕获和运动控制的集成纳米孔和保留阱机制公开(公告)号：US09201057B2
公开(公告)日：2015-12-01
申请号：US13738315
申请日：2013-01-10
IPC分类号： G01N33/487 , C12Q1/68 , B82Y30/00
CPC分类号： G01N33/48721 , B82Y30/00 , C12Q1/6869 , C12Q2565/631
摘要： A mechanism is provided for capturing a molecule via an integrated system. An alternating voltage is applied to a Paul trap device in an electrically conductive solution to generate electric fields. The Paul trap device is integrated with a nanopore device to form the integrated system. Forces from the electric fields of the Paul trap device position the molecule to a nanopore in the nanopore device. A first voltage is applied to the nanopore device to capture the molecule in the nanopore of the nanopore device.
摘要翻译： 提供了用于通过集成系统捕获分子的机制。 将交流电压施加到导电溶液中的保持阱陷阱装置以产生电场。 保存陷阱装置与纳米孔装置集成以形成集成系统。 来自保护陷阱装置的电场的力将分子定位在纳米孔装置中的纳米孔。 将第一电压施加到纳米孔装置以捕获纳米孔装置的纳米孔中的分子。
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7.发明申请DNA SEQUENCING USING MULTIPLE METAL LAYER STRUCTURE WITH DIFFERENT ORGANIC COATINGS FORMING DIFFERENT TRANSIENT BONDINGS TO DNA 有权使用多种金属层结构与不同有机涂层形成不同瞬态结合到DNA的DNA序列公开(公告)号：US20150160159A1
公开(公告)日：2015-06-11
申请号：US14624868
申请日：2015-02-18
发明人： Ali Afzali-Ardakani , Stefan Harrer , Binquan Luan , Hongbo Peng , Stephen M. Rossnagel , Ajay K. Royyuru , Gustavo A. Stolovitzky , Philip S. Waggoner
IPC分类号： G01N27/447
CPC分类号： G01N33/48721 , B82Y15/00 , C12Q1/6869 , G01N27/44743 , G01N27/44791 , Y10S977/924 , C12Q2563/157
摘要： A nanodevice includes a reservoir filled with conductive fluid and a membrane separating the reservoir. A nanopore is formed through the membrane having electrode layers separated by insulating layers. A certain electrode layer has a first type of organic coating and a pair of electrode layers has a second type. The first type of organic coating forms a motion control transient bond to a molecule in the nanopore for motion control, and the second type forms first and second transient bonds to different bonding sites of a base of the molecule. When a voltage is applied to the pair of electrode layers a tunneling current is generated by the base in the nanopore, and the tunneling current travels via the first and second transient bonds formed to be measured as a current signature for distinguishing the base. The motion control transient bond is stronger than first and second transient bonds.
摘要翻译： 纳米器件包括填充有导电流体的储存器和分离储存器的膜。 通过具有由绝缘层分隔的电极层的膜形成纳米孔。 某一电极层具有第一类型的有机涂层，并且一对电极层具有第二类型。 第一类型的有机涂层形成运动控制瞬态键合到用于运动控制的纳米孔中的一个分子，而第二种类型形成与分子的基底的不同键合位点的第一和第二瞬态键。 当对一对电极层施加电压时，由纳米孔中的基底产生隧穿电流，并且隧穿电流通过形成为待测量的第一和第二瞬态键作为用于区分基极的电流签名行进。 运动控制瞬态键比第一和第二瞬态键强。
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公开(公告)号：US20140312003A1
公开(公告)日：2014-10-23
申请号：US13971532
申请日：2013-08-20
CPC分类号： G01N33/48721 , C12Q1/6869 , C23C18/161 , C23C18/1644 , C23C18/1675 , C23C18/42 , C25D3/50 , C25D5/02 , C25D7/00 , C25D7/12 , C25D21/12
摘要： A mechanism is provided for forming a nanodevice. A reservoir is filled with a conductive fluid, and a membrane is formed to separate the reservoir in the nanodevice. The membrane includes an electrode layer having a tunneling junction formed therein. The membrane is formed to have a nanopore formed through one or more other layers of the membrane such that the nanopore is aligned with the tunneling junction of the electrode layer. The tunneling junction of the electrode layer is narrowed to a narrowed size by electroplating or electroless deposition. When a voltage is applied to the electrode layer, a tunneling current is generated by a base in the tunneling junction to be measured as a current signature for distinguishing the base. When an organic coating is formed on an inside surface of the tunneling junction, transient bonds are formed between the electrode layer and the base.
摘要翻译： 提供了用于形成纳米器件的机构。 储存器填充有导电流体，并且形成膜以分离纳米装置中的储存器。 膜包括其中形成有隧道结的电极层。 膜形成为具有通过膜的一个或多个其它层形成的纳米孔，使得纳米孔与电极层的隧道结对准。 电极层的隧道结通过电镀或无电沉积而变窄到窄的尺寸。 当向电极层施加电压时，通过在要测量的隧道结中的基底产生隧道电流作为区分基底的当前签名。 当在隧道结的内表面上形成有机涂层时，在电极层和基底之间形成瞬态结合。
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9.发明申请GRAPHENE TRANSISTOR GATED BY CHARGES THROUGH A NANOPORE FOR BIO-MOLECULAR SENSING AND DNA SEQUENCING 有权用于生物分子传感和DNA测序的纳米级电荷的石墨晶体管公开(公告)号：US20140141521A1
公开(公告)日：2014-05-22
申请号：US14163141
申请日：2014-01-24
发明人： Hongbo Peng , Gustavo A. Stolovitzky , Wenjuan Zhu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , B82Y5/00 , G01N33/48721 , Y10T436/143333
摘要： A technique for a nanodevice is provided. A reservoir is separated into two parts by a membrane. A nanopore is formed through the membrane, and the nanopore connects the two parts of the reservoir. The nanopore and the two parts of the reservoir are filled with ionic buffer. The membrane includes a graphene layer and insulating layers. The graphene layer is wired to first and second metal pads to form a graphene transistor in which transistor current flowing through the graphene transistor is modulated by charges or dipoles passing through the nanopore.
摘要翻译： 提供了一种纳米设备的技术。 通过膜将储存器分成两部分。 通过膜形成纳米孔，并且纳米孔连接储层的两个部分。 纳米孔和储存器的两个部分填充有离子缓冲液。 膜包括石墨烯层和绝缘层。 石墨烯层连接到第一和第二金属焊盘以形成石墨烯晶体管，其中流过石墨烯晶体管的晶体管电流通过电荷或穿过纳米孔的偶极子来调制。
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公开(公告)号：US11161112B2
公开(公告)日：2021-11-02
申请号：US16506826
申请日：2019-07-09
摘要： Techniques for phosphoprotein detection, quantification, and purification using a chip-based pillar array are provided. In one aspect, a method for purifying a protein sample includes: introducing a mixture including the protein sample and an antibody to a nanoDLD array having a plurality of pillars separated by a gap g, wherein the antibody and proteins in the protein sample form antibody-protein complexes having a size that is greater than a size threshold of the nanoDLD array created by the gap g which permits size-based separation of the antibody-protein complexes as the mixture flows through the nanoDLD array; and collecting a purified protein sample containing the antibody-protein complexes from the nanoDLD array. A lab-on-a-chip (LOC) device including the nanoDLD array is also provided.
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