Process for producing optically active 1,3-butanediol
    22.
    发明授权
    Process for producing optically active 1,3-butanediol 失效
    光学活性1,3-丁二醇的制备方法

    公开(公告)号:US5512465A

    公开(公告)日:1996-04-30

    申请号:US247849

    申请日:1994-05-23

    IPC分类号: C12P7/18 C12P41/00

    摘要: Optically active 1,3-butanediol can be obtained by treating an enantiomorphic mixture of 1,3-butanediol with a microorganism or cells thereof which have been ground, acetone-treated, or lyophilized capable of acting on an enantiomorphic mixture of 1,3-butanediol so as to leave (R)- or (S)-1,3-butanediol as such. Further, optically active 1,3-butanediol can be obtained by treating 4-hydroxy-2-butanone with a microorganism or cells thereof which have been ground, acetone-treated, or lyophilized capable of asymmetrically reducing the 4-hydroxy-2-butanone into (R)- or (S)-1,3-butanediol.

    摘要翻译: 光学活性1,3-丁二醇可以通过将1,3-丁二醇与其研磨,丙酮处理或冻干的微生物或其细胞的对映体混合物进行处理,所述微生物或细胞能够作用于1,3-二醇的对映体混合物, (R) - 或(S)-1,3-丁二醇。 此外,光学活性1,3-丁二醇可以通过用已研磨,丙酮处理或冻干的微生物或其细胞处理4-羟基-2-丁酮得到,其能够不对称还原4-羟基-2-丁酮 (R) - 或(S)-1,3-丁二醇。

    Process for the production of optically active 2-hydroxy acid esters
using microbes to reduce the 2-oxo precursor
    24.
    发明授权
    Process for the production of optically active 2-hydroxy acid esters using microbes to reduce the 2-oxo precursor 失效
    使用微生物生产光学活性的2-羟基酸酯以还原2-氧代前体的方法

    公开(公告)号:US5371014A

    公开(公告)日:1994-12-06

    申请号:US139878

    申请日:1993-10-22

    IPC分类号: C12P7/62 C12P41/00

    摘要: An optically active 2-hydroxy acid derivative is produced by treating a 2-oxo acid derivative with a microorganism, which has been optionally treated, capable of asymmetrically reducing said 2-oxo acid derivative into an optically active (R)- or (S)-2-hydroxy acid derivative represented by the formula (II) and recovering the optically active (R)- or (S)-hydroxy acid derivative thus formed. Optically active 2-hydroxy acid derivatives are important intermediates in the synthesis of various drugs such as a remedy for hypertension.

    摘要翻译: 通过用任选处理的能够将所述2-氧代酸衍生物不对称还原成光学活性(R) - 或(S)的微生物的2-氧代酸衍生物来制备光学活性的2-羟基酸衍生物, (II)表示的2-羟基酸衍生物,并回收由此形成的光学活性(R) - 或(S) - 羟基酸衍生物。 光学活性的2-羟基酸衍生物是合成各种药物的重要中间体,例如高血压药物。

    Process for producing optically active 1,3-butanediol
    25.
    发明授权
    Process for producing optically active 1,3-butanediol 失效
    光学活性1,3-丁二醇的制备方法

    公开(公告)号:US5336619A

    公开(公告)日:1994-08-09

    申请号:US778918

    申请日:1991-12-31

    摘要: Optically active 1,3-butanediol can be obtained by treating an enantiomorphic mixture of 1,3-butanediol with a microorganism or cells thereof which have been ground, acetone-treated, or lyophilized, capable of acting on an enantiomorphic mixture of 1,3-butanediol so as to leave (R)- or (S)-1,3-butanediol as such.Further, optically active 1,3-butanediol can be obtained by treating 4-hydroxy-2-butanone with a microorganism or cells thereof which have been ground, acetone-treated, or lyophilized, capable of asymmetrically reducing the 4-hydroxy-2-butanone into (R)- or (S)-1,3-butanediol.

    摘要翻译: 光学活性1,3-丁二醇可以通过将1,3-丁二醇与其研磨,丙酮处理或冻干的微生物或其细胞的对映体混合物进行处理,能够作用于对映体混合物1,3 - 丁二醇,以便留下(R) - 或(S)-1,3-丁二醇。 此外,光学活性1,3-丁二醇可以通过用研磨,丙酮处理或冻干的微生物或其细胞处理4-羟基-2-丁酮,能够不对称还原4-羟基-2-丁酮, 丁酮转化成(R) - 或(S)-1,3-丁二醇。

    Process for producing optically active 1,3-butandiol by asymmetric
assimilation
    26.
    发明授权
    Process for producing optically active 1,3-butandiol by asymmetric assimilation 失效
    通过不对称同化制备光学活性1,3-丁二醇的方法

    公开(公告)号:US5326705A

    公开(公告)日:1994-07-05

    申请号:US989499

    申请日:1992-12-10

    摘要: Optically active 1,3-BUTANEDIOL is produced by contacting microorganisms selected from respective groups of specific genuses having an effect of acting on an enantiomeric mixture of 1,3-butanediols and leaving (R)-1,3-butanediol or (S)-1,3-butanediol in enantiomeric mixture, and collecting the remaining optically active (R)-1,3-butandiol or (S)-1,3-butanediol. Optically active 1,3-butanediol can be produced by an economically excellent and convenient means.

    摘要翻译: 通过使选自具有作用于1,3-丁二醇的对映异构体混合物的作用的特定基因的各组的微生物接触并且留下(R)-1,3-丁二醇或(S) - 并且收集剩余的光学活性(R)-1,3-丁二醇或(S)-1,3-丁二醇。 光学活性的1,3-丁二醇可以通过经济上优良和方便的方法生产。

    Nitrile hydratase and a method for producing amides
    28.
    发明授权
    Nitrile hydratase and a method for producing amides 失效
    腈水解酶和酰胺的制备方法

    公开(公告)号:US07300784B2

    公开(公告)日:2007-11-27

    申请号:US10469427

    申请日:2002-03-01

    CPC分类号: C12P13/02 C12N9/88

    摘要: An objective of the present invention is to provide a nitrile hydratase capable of producing 2-hydroxy-4-methylthiobutyroamide. The present invention provides a novel nitrile hydratase producing α-hydroxyamide using, α-hydroxnitrile as the substrate, and the encoding DNA thereof. The enzyme can be obtained from Rhodococcus sp. Further, the enzymatic activity of the enzyme can he maintained stably during the reaction. The present invention provides a method for producing amide compounds, the method comprising the step of reacting this enzyme to nitrile compounds. According to the present invention, from hydroxy nitrile compounds, corresponding amide compounds can be produced biochemically without reducing the enzyme activity of nitrile hydratase.

    摘要翻译: 本发明的目的是提供能够生产2-羟基-4-甲硫基丁酰胺的腈水合酶。 本发明提供使用α-羟基腈作为底物的新型腈水合酶产生α-羟基酰胺及其编码DNA。 该酶可以从红球菌(Rhodococcus sp。)获得。 此外,酶的酶活性可以在反应期间保持稳定。 本发明提供了一种制备酰胺化合物的方法,所述方法包括使该酶与腈化合物反应的步骤。 根据本发明,由羟基腈化合物可以生物化学地制备相应的酰胺化合物,而不会降低腈水合酶的酶活性。

    Rhodococcus microorganisms and process for producing amide compounds
    30.
    发明授权
    Rhodococcus microorganisms and process for producing amide compounds 失效
    红球菌微生物和生产酰胺化合物的方法

    公开(公告)号:US06699695B1

    公开(公告)日:2004-03-02

    申请号:US09622201

    申请日:2000-08-14

    IPC分类号: C12P1302

    摘要: A nitrile compound having a complicated structure (e.g., 2-hydroxy-4-methylthiobutyronitrile) is converted into an amide compound with high production efficiency, by using a novel microorganism of which the gene 16S rRNA has a specific base sequence. As the microorganism, Rhodococcus sp. Cr4 strain and Rhodococcus sp. Am8 strain or the like is employed.

    摘要翻译: 通过使用基因16S rRNA具有特定碱基序列的新型微生物,将具有复杂结构的腈化合物(例如2-羟基-4-甲基硫代丁腈)转化为高生产效率的酰胺化合物。 作为微生物,红球菌属 Cr4菌株和红球菌属 使用Am8菌株等。