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223 条记录 (耗时 0.065 秒)

    Nucleic acid ligand diagnostic biochip
    21.
    发明授权
    Nucleic acid ligand diagnostic biochip 有权
    核酸配体诊断生物芯片

    公开(公告)号：US07709192B2

    公开(公告)日：2010-05-04

    申请号：US10375487

    申请日：2003-02-27

    申请人： Larry Gold ,  Dominic Zichi

    发明人： Larry Gold ,  Dominic Zichi

    IPC分类号： C12Q1/68 ,  G01N33/53

    CPC分类号： C12Q1/6839 ,  C07B2200/11 ,  C12Q1/6834 ,  C12Q1/6837 ,  C40B40/00 ,  Y10S977/924 ,  C12Q2565/514 ,  C12Q2565/101 ,  C12Q2541/101 ,  C12Q2525/205

    摘要： A nucleic acid ligand “biochip” is disclosed, consisting of a solid support to which one or more specific nucleic acid ligands is attached in a spatially defined manner. Each nucleic acid ligand binds specifically and avidly to a particular target molecule contained within a test mixture, such as a bodily fluid. The target molecules include, but are not limited to, proteins (cellular, viral, bacterial, etc.) hormones, sugars, metabolic byproducts, cofactor, and intermediates, drugs, and toxins. Contacting the test mixture with the biochip leads to the binding of a target molecule to its cognate nucleic acid ligand. The biochip may then be contacted with a reagent(s) that reacts covalently with proteins and not with nucleic acids. Each protein target in the test mixture may then detected by detecting the presence of the reagent at the appropriate address on the biochip.

    摘要翻译： 公开了一种核酸配体“生物芯片”，其由以空间限定的方式连接一个或多个特定核酸配体的固体支持物组成。 每个核酸配体特异性地并且强烈地结合到测试混合物（例如体液）中所含的特定靶分子。 靶分子包括但不限于蛋白质（细胞，病毒，细菌等）激素，糖，代谢副产物，辅因子和中间体，药物和毒素。 将测试混合物与生物芯片接触导致靶分子与其同源核酸配体的结合。 然后生物芯片可以与与蛋白质共价反应的试剂与不与核酸反应的试剂接触。 然后可以通过检测生物芯片上适当地址处的试剂的存在来检测测试混合物中的每个蛋白质靶标。

    Lung Cancer Biomarkers and Uses Thereof
    22.
    发明申请
    Lung Cancer Biomarkers and Uses Thereof 审中-公开
    肺癌生物标志物及其用途

    公开(公告)号：US20100070191A1

    公开(公告)日：2010-03-18

    申请号：US12556480

    申请日：2009-09-09

    申请人： Larry Gold ,  Marty Stanton ,  Edward N. Brody ,  Rachel M. Ostroff ,  Dominic Zichi ,  Alex A.E. Stewart

    发明人： Larry Gold ,  Marty Stanton ,  Edward N. Brody ,  Rachel M. Ostroff ,  Dominic Zichi ,  Alex A.E. Stewart

    IPC分类号： G01N33/574 ,  C12Q1/02 ,  G06F19/00

    CPC分类号： G01N33/57423

    摘要： The present application includes biomarkers, methods, devices, reagents, systems, and kits for the detection and diagnosis of lung cancer. In one aspect, the application provides biomarkers that can be used alone or in various combinations to diagnose lung cancer or permit the differential diagnosis of pulmonary nodules as benign or malignant. In another aspect, methods are provided for diagnosing lung cancer in an individual, where the methods include detecting, in a biological sample from an individual, at least one biomarker value corresponding to at least one biomarker selected from the group of biomarkers provided in Table 1, Col. 2, wherein the individual is classified as having lung cancer, or the likelihood of the individual having lung cancer is determined, based on the at least one biomarker value.

    摘要翻译： 本申请包括用于肺癌的检测和诊断的生物标志物，方法，装置，试剂，系统和试剂盒。 在一个方面，该应用提供可以单独使用或以各种组合使用以诊断肺癌或允许肺结节的鉴别诊断为良性或恶性的生物标志物。 在另一方面，提供了用于诊断个体中的肺癌的方法，其中所述方法包括在来自个体的生物样品中检测至少一种对应于选自表1中提供的生物标志物组的至少一种生物标志物的生物标记值 ，第2栏，其中基于所述至少一种生物标记值，将个体分类为具有肺癌或确定患有肺癌的个体的可能性。

    SELEX AND PHOTOSELEX
    24.
    发明申请
    SELEX AND PHOTOSELEX 有权
    SELEX和PHOTOSELEX

    公开(公告)号：US20090098549A1

    公开(公告)日：2009-04-16

    申请号：US12175388

    申请日：2008-07-17

    申请人： Daniel J. Schneider ,  Sheri K. Wilcox ,  Dominic Zichi ,  Dan Nieuwlandt ,  Jeff Carter ,  Larry Gold

    发明人： Daniel J. Schneider ,  Sheri K. Wilcox ,  Dominic Zichi ,  Dan Nieuwlandt ,  Jeff Carter ,  Larry Gold

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6811 ,  C12N15/1048 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/13 ,  C12Q2541/101 ,  C12Q2525/205 ,  C12Q2525/117

    摘要： The present disclosure describes improved SELEX methods for generating nucleic acid ligands that are capable of binding to target molecules and improved photoSELEX methods for generating photoreactive nucleic acid ligands that are capable of both binding and covalently crosslinking to target molecules. The disclosure further describes nucleic acid libraries having expanded physical and chemical properties and their use in SELEX and photoSELEX; methods for increasing the crosslinking efficiencies of photoaptamers; methods for producing photoaptamers having selective modifications that enhance functionality and minimize non-specific photoreactions; and methods for generating truncated nucleic acid ligands from nucleic acid ligands of longer length. The disclosure further describes aptamers and photoaptamers obtained by using any of the foregoing.

    摘要翻译： 本公开描述了用于产生能够结合靶分子的核酸配体的改进的SELEX方法和用于产生能够与靶分子结合和共价交联的光反应性核酸配体的改进的photoSELEX方法。 本公开进一步描述了具有扩展的物理和化学性质及其在SELEX和photoSELEX中的用途的核酸文库; 提高光催化剂交联效率的方法; 制备具有增强功能并使非特异性光反应最小化的选择性修饰的光致抗体的方法; 以及用于从较长长度的核酸配体产生截短的核酸配体的方法。 本公开进一步描述了通过使用上述任何一种获得的适体和光致抗体。

    SYSTEMATIC EVOLUTION OF LIGANDS BY EXPONENTIAL ENRICHMENT: BLENDED SELEX
    25.
    发明申请
    SYSTEMATIC EVOLUTION OF LIGANDS BY EXPONENTIAL ENRICHMENT: BLENDED SELEX 审中-公开
    通过经验丰富的配方的系统演变：混合SELEX

    公开(公告)号：US20070134715A1

    公开(公告)日：2007-06-14

    申请号：US11668337

    申请日：2007-01-29

    申请人： Greg Biesecker ,  Sumedha Jayasena ,  Larry Gold ,  Drew Smith ,  Gary Kirschenheuter

    发明人： Greg Biesecker ,  Sumedha Jayasena ,  Larry Gold ,  Drew Smith ,  Gary Kirschenheuter

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12P19/34 ,  A61K47/64 ,  C07K5/1008 ,  C07K19/00 ,  C12N15/1048

    摘要： A method is described for generating blended nucleic acid ligands containing non-nucleic acid functional units. Specifically, a SELEX identified RNA ligand to the integrin gpIIbIIIa is conjugated to the peptide Gly-Arg-Gly-Asp-Thr-Pro (SEQ ID NO:1). This blended RNA ligand inhibits the biological activity of gpIIbIIIa with high specificity. Also described is a single-stranded DNA ligand to elastase coupled to N-methoxysuccinyl-Ala-Ala-Pro-Val-chloromethyl ketone (SEQ ID NO:2). This elastase blended nucleic acid ligand inhibits the biological activity of elastase.

    摘要翻译： 描述了一种用于产生含有非核酸功能单元的混合核酸配体的方法。 具体地，将SELEX鉴定的整合素gpIIbIIIa的RNA配体与肽Gly-Arg-Gly-Asp-Thr-Pro（SEQ ID NO：1）缀合。 这种混合RNA配体以高特异性抑制gpIIbIIIa的生物活性。 还描述了与N-甲氧基琥珀酰基-Ala-Ala-Pro-Val-氯甲基酮（SEQ ID NO：2）偶联的弹性蛋白酶的单链DNA配体。 该弹性蛋白酶共混核酸配体抑制弹性蛋白酶的生物活性。

    Systematic evolution of ligands by exponential enrichment: blended SELEX
    26.
    发明授权
    Systematic evolution of ligands by exponential enrichment: blended SELEX 失效
    配体通过指数富集系统演化：混合SELEX

    公开(公告)号：US07176295B2

    公开(公告)日：2007-02-13

    申请号：US10263456

    申请日：2002-10-02

    申请人： Gregory Biesecker ,  Sumeda Jayasena ,  Larry Gold ,  Drew Smith ,  Gary P. Kirschenheuter

    发明人： Gregory Biesecker ,  Sumeda Jayasena ,  Larry Gold ,  Drew Smith ,  Gary P. Kirschenheuter

    IPC分类号： C07H21/04 ,  C12Q1/68 ,  C12P19/34 ,  C07H21/02

    CPC分类号： C12P19/34 ,  A61K47/64 ,  C07K5/1008 ,  C07K19/00 ,  C12N15/1048

    摘要： A method is described for generating blended nucleic acid ligands containing non-nucleic acid functional units. Specifically, a SELEX identified RNA ligand to the integrin gpIIbIIIa is conjugated to the peptide Gly-Arg-Gly-Asp-Thr-Pro (SEQ ID NO:1). This blended RNA ligand inhibits the biological activity of gpIIbIIIa with high specificity. Also described is a single-stranded DNA ligand to elastase coupled to N-methoxysuccinyl-Ala-Ala-Pro-Val-chloromethyl ketone (SEQ ID NO:2). This elastase blended nucleic acid ligand inhibits the biological activity of elastase.

    摘要翻译： 描述了一种用于产生含有非核酸功能单元的混合核酸配体的方法。 具体地，将SELEX鉴定的整合素gpIIbIIIa的RNA配体与肽Gly-Arg-Gly-Asp-Thr-Pro（SEQ ID NO：1）缀合。 这种混合RNA配体以高特异性抑制gpIIbIIIa的生物活性。 还描述了与N-甲氧基琥珀酰基-Ala-Ala-Pro-Val-氯甲基酮（SEQ ID NO：2）偶联的弹性蛋白酶的单链DNA配体。 该弹性蛋白酶共混核酸配体抑制弹性蛋白酶的生物活性。

    Bivalent binding molecules of 7 transmembrane G protein-coupled receptors
    27.
    发明申请
    Bivalent binding molecules of 7 transmembrane G protein-coupled receptors 审中-公开

    公开(公告)号：US20060147987A1

    公开(公告)日：2006-07-06

    申请号：US11370087

    申请日：2006-03-06

    申请人： Larry Gold

    发明人： Larry Gold

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  C07H21/04

    CPC分类号： G01N33/74 ,  C12Q1/6811 ,  G01N2500/00 ,  C12Q2541/101

    摘要： Described herein are methods for identifying and preparing bivalent binding molecules to 7 transmembrane G protein-coupled receptors. The methods disclosed herein are based on the SELEX method for generating high affinity nucleic acid ligands. SELEX is an acronym for Systematic Evolution of Ligands by EXponential enrichment. The methods of this invention combine two or more binding domains to two or more different epitopes of the same 7 transmembrane G protein-coupled receptor. These bivalent binding molecules are useful as therapeutic and diagnostic agents.

    Determining non-nucleic acid molecule binding to target by competition with nucleic acid ligands
    30.
    发明申请
    Determining non-nucleic acid molecule binding to target by competition with nucleic acid ligands 有权
    通过与核酸配体竞争来测定与核酸分子结合的非核酸分子

    公开(公告)号：US20050048521A1

    公开(公告)日：2005-03-03

    申请号：US10746565

    申请日：2003-12-24

    申请人： Nebojsa Janjic ,  Larry Gold

    发明人： Nebojsa Janjic ,  Larry Gold

    IPC分类号： G01N33/50 ,  C12Q1/68 ,  G01N33/15 ,  G01N33/53 ,  G01N33/566 ,  G01N37/00 ,  G01N33/543

    CPC分类号： G01N33/53 ,  C12Q1/6811 ,  Y10S530/812 ,  C12Q2545/107 ,  C12Q2565/518

    摘要： A competitive binding assay is used to determine whether a non-nucleic acid molecule from a library of non-nucleic acid molecules binds to a target. The non-nucleic acid molecule competes with a labeled nucleic acid ligand for binding to the target which may be immobilized. Detecting displacement of labeled nucleic acid ligand from a complex of the labeled nucleic acid ligand and the target determines binding of the non-nucleic acid molecule to the target. The nucleic acid ligand may be immobilized and contacted with a labeled target to form a complex. Adding a non-nucleic acid molecule to the complex displaces labeled target from the complex, and detecting displacement of the labeled target determines binding of the non-nucleic acid molecule to the target. Labeled nucleic acid ligand or labeled target displaced from or remaining in the complex can be detected for detecting displacement. Nucleic acid ligands that bind to the target are identified by the SELEX method. The assay provides a high throughput screening method for determining whether a non-nucleic acid molecule binds to a target.

    摘要翻译： 使用竞争性结合测定来确定非核酸分子的文库中的非核酸分子是否与靶标结合。 非核酸分子与标记的核酸配体竞争结合靶，其可以被固定化。 检测标记的核酸配体从标记的核酸配体和靶的复合物的置换确定非核酸分子与靶标的结合。 核酸配体可以被固定化并与标记的靶接触以形成复合物。 向复合物中加入非核酸分子从复合物置换标记的靶，并且检测标记的靶的置换确定非核酸分子与靶的结合。 可以检测标记的核酸配体或从复合物中残留的残留的标记的靶标，以检测位移。 通过SELEX法鉴定与靶标结合的核酸配体。 该测定提供了用于确定非核酸分子是否结合靶的高通量筛选方法。