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21.发明授权Mutated D-aminotransferase and method for producing optically active glutamic acid derivatives using the same 失效突变的D-氨基转移酶及其制备使用其的光学活性谷氨酸衍生物的方法公开(公告)号：US08669080B2
公开(公告)日：2014-03-11
申请号：US12055605
申请日：2008-03-26
CPC分类号： C12N9/1096 , C12P17/10
摘要： A D-aminotransferase can be modified so as to efficiently produce (2R,4R)-monatin having high sweetness intensity from 4-(indol-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid by mutating the amino acid sequence of a wild-type D-aminotransferase represented in SEQ ID NO:4.
摘要翻译： 可以修饰D-氨基转移酶，以便通过将氨基酸序列的突变来有效地从4-（吲哚-3-基甲基）-4-羟基-2-氧代戊二酸生成具有高甜度强度的（2R，4R） SEQ ID NO：4所示的野生型D-氨基转移酶。
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22.发明申请NOVEL OXIDASE GENE AND METHOD FOR PRODUCING 3-INDOLE-PYRUVIC ACID BY UTILIZING THE GENE 有权新型氧化酶基因及利用该基因生产3-吲哚酸的方法公开(公告)号：US20130084610A1
公开(公告)日：2013-04-04
申请号：US13684888
申请日：2012-11-26
申请人： Yasuaki TAKAURA , Seiichi Hara , Toshiki Taba , Shunichi Suzuki , Masakazu Sugiyama , Kunihiko Watanabe , Kenzo Yokozeki
发明人： Yasuaki TAKAURA , Seiichi Hara , Toshiki Taba , Shunichi Suzuki , Masakazu Sugiyama , Kunihiko Watanabe , Kenzo Yokozeki
CPC分类号： C12P17/10 , C12N9/0022
摘要： It is an object of the present invention to provide a procedure for realizing inexpensive and simple production of 3-indole-pyruvic acid. A transformant is made using a polynucleotide having a specific nucleotide sequence encoding a protein having an oxidase activity, and oxidase is generated by culturing the transformant in a medium to accumulate the oxidase in the medium and/or the transformant. Further, tryptophan is converted into 3-indole-pyruvic acid in the presence of the transformant and/or a culture thereof to produce 3-indole-pyruvic acid.
摘要翻译： 本发明的目的是提供一种实现廉价且简单的3-吲哚 - 丙酮酸生产的方法。 使用具有编码具有氧化酶活性的蛋白质的特异性核苷酸序列的多核苷酸制备转化体，通过在培养基中培养转化体以在培养基和/或转化体中积累氧化酶而产生氧化酶。 此外，在转化体和/或其培养物存在下，将色氨酸转化为3-吲哚 - 丙酮酸，生成3-吲哚 - 丙酮酸。
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23.发明授权公开(公告)号：US08048647B2
公开(公告)日：2011-11-01
申请号：US12635994
申请日：2009-12-11
摘要： The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and aldolase used for these methods. As such, the present invention enables the synthesis of 4-(Indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid with high optical purity, which is useful as an intermediate in the synthesis of optically active monatin, from indole pyruvic acid and pyruvic acid (or oxaloacetic acid).
摘要翻译： 本发明涉及一种用于制备光学活性的IHOG的方法，其可以用于制备莫纳甜。 本发明还涉及用于制备光学活性莫纳甜的方法和用于这些方法的醛缩酶。 因此，本发明能够合成具有高光学纯度的4-（吲哚-3-基甲基）-4-羟基-2-氧代戊二酸，其可用作在光学活性莫纳甜的合成中的吲哚丙酮酸的中间体 酸和丙酮酸（或草酰乙酸）。
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24.发明授权公开(公告)号：US07371549B2
公开(公告)日：2008-05-13
申请号：US11362915
申请日：2006-02-28
摘要： The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and aldolase used for these methods. As such, the present invention enables the synthesis of 4-(Indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid with high optical purity, which is useful as an intermediate in the synthesis of optically active monatin, from indole pyruvic acid and pyruvic acid (or oxaloacetic acid).
摘要翻译： 本发明涉及一种用于制备光学活性的IHOG的方法，其可以用于制备莫纳甜。 本发明还涉及用于制备光学活性莫纳甜的方法和用于这些方法的醛缩酶。 因此，本发明能够合成具有高光学纯度的4-（吲哚-3-基甲基）-4-羟基-2-氧代戊二酸，其可用作在光学活性莫纳甜的合成中的吲哚丙酮酸的中间体 酸和丙酮酸（或草酰乙酸）。
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公开(公告)号：US20070292916A1
公开(公告)日：2007-12-20
申请号：US11765926
申请日：2007-06-20
申请人： Isao ABE , Rie Takeshita , Seiichi Hara , Sonoko Suzuki , Kenzo Yokozeki , Masakazu Sugiyama , Shunichi Suzuki , Kunihiko Watanabe , Nobuhisa Shimba , Takefumi Nakamura , Uno Tagami , Yuya Kodama , Hiromi Onoye , Reiko Yuuji , Eiichiro Suzuki , Tatsuki Kashiwagi , Ningchun Xu , Yuko Kai
发明人： Isao ABE , Rie Takeshita , Seiichi Hara , Sonoko Suzuki , Kenzo Yokozeki , Masakazu Sugiyama , Shunichi Suzuki , Kunihiko Watanabe , Nobuhisa Shimba , Takefumi Nakamura , Uno Tagami , Yuya Kodama , Hiromi Onoye , Reiko Yuuji , Eiichiro Suzuki , Tatsuki Kashiwagi , Ningchun Xu , Yuko Kai
CPC分类号： C07K14/00 , C07K14/195 , C12N9/93
摘要： The present invention aims at providing an excellent peptide-synthesizing protein and a method for efficiently producing a peptide. The peptide is synthesized by reacting an amine component and a carboxy component in the presence of at least one of proteins shown in the following (I) and (II). (I) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations 1 to 68, and the mutations 239 to 290 and 324 to 377 in an amino acid sequence of SEQ ID NO:2. (II) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations L1 to L335 and M1 to M642 in an amino acid sequence of SEQ ID NO:208
摘要翻译： 本发明的目的在于提供优异的肽合成蛋白质和有效生产肽的方法。 在下列（I）和（II）所示的至少一种蛋白质存在下，使胺组分和羧基组分反应合成肽。 （I）具有包含SEQ ID NO：2的氨基酸序列中的任何突变1至68和突变239至290和324至377的一个或多个突变的氨基酸序列的突变蛋白。 （II）具有包含SEQ ID NO：208的氨基酸序列中的任何突变L1至L335和M1至M642的一个或多个突变的氨基酸序列的突变蛋白
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26.发明授权公开(公告)号：US07241599B2
公开(公告)日：2007-07-10
申请号：US11146093
申请日：2005-06-07
摘要： The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and aldolase used for these methods. As such, the present invention enables the synthesis of 4-(Indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid with high optical purity, which is useful as an intermediate in the synthesis of optically active monatin, from indole pyruvic acid and pyruvic acid (or oxaloacetic acid).
摘要翻译： 本发明涉及一种用于制备光学活性的IHOG的方法，其可以用于制备莫纳甜。 本发明还涉及用于制备光学活性莫纳甜的方法和用于这些方法的醛缩酶。 因此，本发明能够合成具有高光学纯度的4-（吲哚-3-基甲基）-4-羟基-2-氧代戊二酸，其可用作在光学活性莫纳甜的合成中的吲哚丙酮酸的中间体 酸和丙酮酸（或草酰乙酸）。
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27.发明授权Mutated D-aminotransferase and method for producing optically active glutamic acid derivatives using the same 失效突变的D-氨基转移酶及其制备使用其的光学活性谷氨酸衍生物的方法公开(公告)号：US08058034B2
公开(公告)日：2011-11-15
申请号：US12758433
申请日：2010-04-12
CPC分类号： C12N9/1096 , C12P17/10
摘要： A D-aminotransferase can be modified so as to efficiently produce (2R,4R)-monatin having high sweetness intensity from 4-(indol-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid by substituting an amino acid at least at one of positions (positions 100, 180 to 183, 243 and 244) involved in efficiently producing the (2R,4R)-monatin in an amino acid sequence of a wild-type D-aminotransferase represented in SEQ ID NO:2.
摘要翻译： 可以修饰D-氨基转移酶，以便通过用氨基酸至少在4-（吲哚-3-基甲基）-4-羟基-2-氧代戊二酸代替氨基酸，有效地产生具有高甜度强度的（2R，4R） 在SEQ ID NO：2所示的野生型D-氨基转移酶的氨基酸序列中有效产生（2R，4R） - 单抗的位置（位置100,180〜183,243和244）之一。
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28.发明申请NOVEL ALDOLASE, AND METHOD FOR PRODUCING OPTICALLY ACTIVE IHOG AND MONATIN 失效新型ALDOLASE和用于生产光学活性的IHOG和MONATIN的方法公开(公告)号：US20090258403A1
公开(公告)日：2009-10-15
申请号：US11953372
申请日：2007-12-10
IPC分类号： C12P17/10
摘要： The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and aldolase used for these methods. As such, the present invention enables the synthesis of 4-(Indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid with high optical purity, which is useful as an intermediate in the synthesis of optically active monatin, from indole pyruvic acid and pyruvic acid (or oxaloacetic acid).
摘要翻译： 本发明涉及一种用于制备光学活性的IHOG的方法，其可以用于制备莫纳甜。 本发明还涉及用于制备光学活性莫纳甜的方法和用于这些方法的醛缩酶。 因此，本发明能够合成具有高光学纯度的4-（吲哚-3-基甲基）-4-羟基-2-氧代戊二酸，其可用作在光学活性莫纳甜的合成中的吲哚丙酮酸的中间体 酸和丙酮酸（或草酰乙酸）。
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29.发明申请NOVEL ALDOLASE AND PRODUCTION PROCESS OF SUBSTITUTED ALPHA-KETO ACIDS 失效新型ALDOLASE和替代ALPHA-KETO酸的生产方法公开(公告)号：US20080318290A1
公开(公告)日：2008-12-25
申请号：US11960116
申请日：2007-12-19
摘要： 4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and/or oxalacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.
摘要翻译： 可用作合成莫纳甜的中间体的4-（吲哚-3-基甲基）-4-羟基-2-氧戊二酸，可以由吲哚丙酮酸和丙酮酸（和/或草乙酸）通过使用 衍生自假单胞菌属，欧文氏菌属，黄杆菌属或黄单胞菌属的新型醛缩酶。
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公开(公告)号：US20070190602A1
公开(公告)日：2007-08-16
申请号：US11316926
申请日：2005-12-27
申请人： Isao Abe , Rie Takeshita , Seiichi Hara , Sonoko Suzuki , Kenzo Yokozeki , Masakazu Sugiyama , Shunichi Suzuki , Kunihiko Watanabe , Nobuhisa Shimba , Takefumi Nakamura , Uno Tagami , Yuya Kodama , Hiromi Onoye , Reiko Yuuji , Eiichiro Suzuki , Tatsuki Kashiwagi , Ningchun Xu , Yuko Kai
发明人： Isao Abe , Rie Takeshita , Seiichi Hara , Sonoko Suzuki , Kenzo Yokozeki , Masakazu Sugiyama , Shunichi Suzuki , Kunihiko Watanabe , Nobuhisa Shimba , Takefumi Nakamura , Uno Tagami , Yuya Kodama , Hiromi Onoye , Reiko Yuuji , Eiichiro Suzuki , Tatsuki Kashiwagi , Ningchun Xu , Yuko Kai
CPC分类号： C12N9/93
摘要： The present invention aims at providing an excellent peptide-synthesizing protein and a method for efficiently producing a peptide. The peptide is synthesized by reacting an amine component and a carboxy component in the presence of at least one of proteins shown in the following (I) and (II).(I) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations 1 to 68, and the mutations 239 to 290 and 324 to 377 in an amino acid sequence of SEQ ID NO:2.(II) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations L1 to L335 and M1 to M642 in an amino acid sequence of SEQ ID NO:208
摘要翻译： 本发明的目的在于提供优异的肽合成蛋白质和有效生产肽的方法。 在下列（I）和（II）所示的至少一种蛋白质存在下，使胺组分和羧基组分反应合成肽。 （I）具有包含SEQ ID NO：2的氨基酸序列中的任何突变1至68和突变239至290和324至377的一个或多个突变的氨基酸序列的突变蛋白。 （II）具有包含SEQ ID NO：208的氨基酸序列中的任何突变L1至L335和M1至M642的一个或多个突变的氨基酸序列的突变蛋白
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