公开(公告)号：US20200010838A1

公开(公告)日：2020-01-09

申请号：US16516025

申请日：2019-07-18

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Whitney R. Adams, JR. ,  Brian J. Martinell ,  Jyoti R. Rout ,  Edward J. Williams

IPC: C12N15/82

Abstract: The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.

公开(公告)号：US20180023090A1

公开(公告)日：2018-01-25

申请号：US15638200

申请日：2017-06-29

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Brian J. Martinell ,  Michael W. Petersen ,  David A. Somers ,  Yuechun Wan ,  Edward Williams ,  Xudong Ye

IPC: C12N15/82 ,  C12N5/04 ,  C12N9/10 ,  G01N33/00 ,  A01H4/00

CPC classification number: C12N15/8201 ,  A01H4/003 ,  A01H4/008 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  C12Y203/01081 ,  G01N33/0098

Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

公开(公告)号：US09714428B2

公开(公告)日：2017-07-25

申请号：US14507744

申请日：2014-10-06

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Brian J. Martinell ,  Michael W. Petersen ,  David A. Somers ,  Yuechun Wan ,  Edward Williams ,  Xudong Ye

IPC: C12N15/82 ,  A01H1/04 ,  A01H4/00 ,  C12N5/04 ,  G01N33/00 ,  C12N9/10

CPC classification number: C12N15/8201 ,  A01H4/003 ,  A01H4/008 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  C12Y203/01081 ,  G01N33/0098

Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

公开(公告)号：US20150113683A1

公开(公告)日：2015-04-23

申请号：US14507744

申请日：2014-10-06

Applicant: Monsanto Technology LLC

Inventor： Brian J. Martinell ,  Michael W. Petersen ,  David A. Somers ,  Yuechun Wan ,  Edward Williams ,  Xudong Ye

IPC: C12N15/82 ,  C12N9/10

CPC classification number: C12N15/8201 ,  A01H4/003 ,  A01H4/008 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  C12Y203/01081 ,  G01N33/0098

Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

公开(公告)号：US20130198899A1

公开(公告)日：2013-08-01

申请号：US13750977

申请日：2013-01-25

Applicant: Monsanto Technology LLC

Inventor： Beth Jo Calabotta ,  Kevin Lee Deppermann ,  Erik D. Dersch ,  Jerald D. Heise ,  Angela Ranae Koestel ,  Cindy L. Ludwig ,  Brian J. Martinell ,  Edward Williams

IPC: C12N5/04 ,  G01N33/00

CPC classification number: C12N15/8201 ,  A01H4/003 ,  A01H4/008 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  C12Y203/01081 ,  G01N33/0098

Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

公开(公告)号：US11713465B2

公开(公告)日：2023-08-01

申请号：US14550694

申请日：2014-11-21

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： David R. Duncan ,  Sarah Heifner ,  David Kelm ,  Brian J. Martinell ,  Lorena Moeller ,  Anatoly Rivlin ,  Rebecca Rode ,  Xudong Ye ,  Ashok Shrawat ,  Yurong Chen

IPC: C12N15/00 ,  C12N15/82

CPC classification number: C12N15/8205 ,  C12N15/8209

Abstract: The present invention provides methods for the transformation of viable explants from wheat seeds to permit production of transgenic wheat plants. The present invention also relates to methods for producing such explants and related embodiments.

公开(公告)号：US20230057290A1

公开(公告)日：2023-02-23

申请号：US17818913

申请日：2022-08-10

Applicant: Monsanto Technology LLC

Inventor： Brian J. Martinell ,  Anna Mary O'Keefe ,  David Alan Somers ,  Edward James Williams ,  Xudong Ye

IPC: C12N15/82

Abstract: Methods and compositions for plastid transformation and regeneration or development of transplastomic plants are provided. Embryo explants may be excised from seeds, and their meristematic tissue may be transformed directly without initiation of any callus phase before and/or after transformation. The present methods may be performed with fewer culturing steps relative to conventional methods, thereby enabling more rapid and efficient production of targeted transplastomic events in plants.

公开(公告)号：US11566252B2

公开(公告)日：2023-01-31

申请号：US17520352

申请日：2021-11-05

Applicant: Monsanto Technology LLC

Inventor： Beth Jo Calabotta ,  Kevin Lee Deppermann ,  Erik D. Dersch ,  Jerald D. Heise ,  Angela Ranae Koestel ,  Cindy L. Ludwig ,  Brian J. Martinell ,  Edward Williams

IPC: C12N15/82 ,  C12N5/00 ,  A01H4/00 ,  C12N5/04 ,  G01N33/00 ,  C12N9/10 ,  A01H6/20 ,  A01H6/54 ,  A01H6/46 ,  A01H6/60 ,  C12N1/20 ,  C12R1/41

Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

公开(公告)号：US20220340916A1

公开(公告)日：2022-10-27

申请号：US14550694

申请日：2014-11-21

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： David R. Duncan ,  Sarah Heifner ,  David Kelm ,  Brian J. Martinell ,  Lorena Moeller ,  Anatoly Rivlin ,  Rebecca Rode ,  Xudong Ye ,  Ashok Shrawat ,  Yurong Chen

IPC: C12N15/82

Abstract: The present invention provides methods for the transformation of viable explants from wheat seeds to permit production of transgenic wheat plants. The present invention also relates to methods for producing such explants and related embodiments.

公开(公告)号：US11477954B2

公开(公告)日：2022-10-25

申请号：US17124032

申请日：2020-12-16

Applicant: Monsanto Technology LLC

Inventor： Whitney R. Adams, Jr. ,  Brandon Davis ,  Lubomyr Kucher ,  Brian J. Martinell ,  Jyoti R. Rout ,  Brenda A. Lowe

IPC: B03D1/24 ,  B03D1/14 ,  B01F3/04 ,  A01H4/00

Abstract: The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.