公开(公告)号：US5462854A

公开(公告)日：1995-10-31

申请号：US50681

申请日：1993-04-19

申请人： Peter J. Coassin ,  Kenneth D. Konrad ,  Jang B. Rampal ,  Ronald M. Cook

发明人： Peter J. Coassin ,  Kenneth D. Konrad ,  Jang B. Rampal ,  Ronald M. Cook

IPC分类号： C12N15/09 ,  C07H21/00 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/68 ,  C12P19/34

CPC分类号： C07H21/00 ,  C12N15/1034 ,  C12Q1/6853 ,  C12Q1/6862

摘要： Inverse Linkage Oligonucleotides ("ILO") useful in enzymatic process are disclosed. Particularly preferred ILOs are amenable to enzymatic elongation from either, or most preferably, both termini. In a particularly preferred embodiment, each terminus of an ILO has an enzymatically functional 3' group. Accordingly, under appropriate conditions and in the presence of, e.g., dNTPs, enzyme, sample DNA, and ILO comprising a first region complementary to a first region of the sample DNA and a second region complementary to a second, different region of the sample DNA, exponential amplification of the sample DNA can be effectuated.

摘要翻译： 公开了可用于酶促过程的反向连接寡核苷酸（“ILO”）。 特别优选的ILO适于从两末端或最优选两个末端的酶促延伸。 在特别优选的实施方案中，国际劳工组织的每个末端具有酶功能3'基团。 因此，在适当的条件下和在例如dNTPs，酶，样品DNA和ILO的存在下，包含与样品DNA的第一区域互补的第一区域和与样品DNA的第二个不同区域互补的第二区域 ，可以实现样品DNA的指数扩增。

公开(公告)号：US4474947A

公开(公告)日：1984-10-02

申请号：US366537

申请日：1982-04-08

申请人： Derek Hudson ,  Ronald M. Cook

发明人： Derek Hudson ,  Ronald M. Cook

IPC分类号： C07D235/08 ,  C07D249/18 ,  C07F9/6506 ,  C07F9/6518 ,  C07H21/00 ,  C07H19/10

CPC分类号： C07H21/00 ,  C07D235/08 ,  C07D249/18 ,  C07F9/65068 ,  C07F9/65188

摘要： Benzazoles, including benzatriazoles and benzimidazoles are employed in phospate triester oligonucleotide synthesis. The benzazoles form arylbenzazolide coupling agents, and phosphorodibenzazolides. Processes employing these reagents are also disclosed.

摘要翻译： 苯并唑，包括苯并三唑和苯并咪唑，用于磷酸三酯寡核苷酸合成。 苯并唑形成芳基苯并唑化偶合剂和磷酰苯并唑。 还公开了使用这些试剂的方法。

公开(公告)号：US06761855B1

公开(公告)日：2004-07-13

申请号：US10011966

申请日：2001-11-05

申请人： Ronald M. Cook ,  Rand Dill

发明人： Ronald M. Cook ,  Rand Dill

IPC分类号： G01N3060

CPC分类号： G01N30/6052 ,  G01N30/461 ,  G01N30/466 ,  G01N30/603 ,  G01N30/6039 ,  G01N30/6043 ,  G01N30/6047 ,  G01N30/6091

摘要： The present invention is directed to an improved column for use in solid phase synthesis or purification. In a specific embodiment, the column is a disposable plastic column for use in solid phase synthesis or purification of complex chemicals, such as biomolecules and more specifically oligonucleotides. In some embodiments, the column has a top orifice with a sufficient diameter so that a fluid line or a multiple fluid line bundle may dispense fluids into the column with great efficiency. The column has an upper cavity portion configured and sized to render it compatible with dispensing pippetors, so that it can be used as a pipette tip or a pipettor can be used to aspirate the column. The column has a lower cavity portion with a shoulder for ready placement of a lower frit to contain the solid support in a central cavity portion of the column. An upper frit can be conveniently placed in the central cavity portion to seal the solid phase resin. The lower end tip of the column is configured as a luer-type fitting to provide a male luer connection. The upper cavity portion is configured to interface with the male luer of another column, so that two or more columns to be connected in series.

摘要翻译： 本发明涉及一种用于固相合成或纯化的改进柱。 在一个具体的实施方案中，该柱是用于固相合成或纯化复合化学品如生物分子，更具体地说是寡核苷酸的一次性塑料柱。 在一些实施方案中，塔具有足够直径的顶部孔，使得流体管线或多个流体管线束可以以高效率将流体分配到塔中。 该柱具有上空腔部分，其被构造和定尺寸成使其与分配吸管器相容，使得其可以用作移液管尖端或移液器可以用于抽吸柱。 该柱具有带有肩部的下部空腔部分，用于准备放置下部玻璃料以将固体支撑物容纳在该柱的中心空腔部分中。 上部玻璃料可以方便地放置在中央空腔部分中以密封固相树脂。 柱的下端尖端被配置为鲁尔型配件以提供公路厄尔连接。 上空腔部分被配置为与另一列的阳路厄接口，使得两个或更多个串联连接。

公开(公告)号：US20140323325A1

公开(公告)日：2014-10-30

申请号：US13999508

申请日：2014-03-04

申请人： Marc Beal ,  Ronald M. Cook ,  Peter Jay Coassin ,  Arturo Orjalo ,  Jekwan Ryu

发明人： Marc Beal ,  Ronald M. Cook ,  Peter Jay Coassin ,  Arturo Orjalo ,  Jekwan Ryu

IPC分类号： C12Q1/68 ,  G01N21/64

CPC分类号： C12Q1/6841 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/158 ,  G01N21/6428 ,  G01N21/6458 ,  G02B21/0004 ,  G02B21/0096 ,  G02B21/082 ,  G02B21/16 ,  G02B21/18 ,  C12Q2537/143 ,  C12Q2543/10 ,  C12Q2563/107 ,  C12Q2565/601

摘要： The present invention generally relates to imaging single molecules, or one or more collections of single molecules, and methods related to the imaging. In a method aspect, the present invention provides a method of imaging single molecules. The method comprises the steps of: a) exposing a test sample to a probe, wherein the probe comprises a first portion that specifically binds to a target molecule and a second portion that is detectable as the result of one or more chemical groups that interact with light at one or more wavelengths, wherein the probe binds to a target molecule to provide a complex; b) exposing the complex to one or more wavelengths of light that interact with the one or more chemical groups; c) detecting a result from the interacting of one or more wavelengths of light that interact with the one or more chemical groups to provide an image of one or more single molecules. The image possesses a resolution better than 450 nm over a view field area of at least 1×105 μm2, and wherein the image is obtained in a single detection step without variation of any detection settings.

摘要翻译： 本发明一般涉及成像单分子，或单个分子的一个或多个集合，以及与成像有关的方法。 在方法方面，本发明提供了单分子成像的方法。 该方法包括以下步骤：a）将测试样品暴露于探针，其中所述探针包含特异性结合靶分子的第一部分和作为一个或多个化学基团相互作用的结果可检测的第二部分 一个或多个波长的光，其中所述探针结合靶分子以提供复合物; b）将所述复合物暴露于与所述一个或多个化学基团相互作用的一个或多个波长的光; c）检测与一个或多个化学基团相互作用的一种或多种波长的光的相互作用的结果，以提供一个或多个单个分子的图像。 该图像在至少1×105μm2的视野区域中的分辨率优于450nm，并且其中在单个检测步骤中获得图像而不改变任何检测设置。

公开(公告)号：US07635598B2

公开(公告)日：2009-12-22

申请号：US11177998

申请日：2005-07-08

申请人： Ronald M. Cook ,  Eliana Saxon Armstrong ,  Hans E. Johansson

发明人： Ronald M. Cook ,  Eliana Saxon Armstrong ,  Hans E. Johansson

IPC分类号： G01N33/533 ,  G01N33/53 ,  C12Q1/26 ,  C12N1/21 ,  C12P21/00

CPC分类号： C12Q1/26 ,  C09B11/08 ,  C12Q1/6818 ,  C12Q1/6897 ,  G01N33/542 ,  G01N2333/90209 ,  Y10S436/903 ,  Y10T436/13

摘要： In one aspect, the invention provides fluorescent probes and assays. The probes include a fluorophore-quencher pair that undergoes a switch from dark to fluorescent in response to a reaction of the quencher. The switch of the probe from dark to fluorescent is typically mediated by an enzyme that acts directly or indirectly on the quencher, interfering with its ability to quench fluorescence emission from the fluorophore.In another aspect, the invention provides a reporter gene assay system and methods of using this system. The assay system includes a fluorophore-quencher probe and an enzyme that acts directly or indirectly on the quencher, increasing the fluorescent emission of the fluorophore. In still other aspects, the invention provides nucleic acid constructs and cells expressing the peptide products of these constructs.In assays of the invention, the presence of a target substance is detected by the switching of fluorescence mediated by the change in oxidation state of the quencher. The assay systems are of use in numerous assay formats, e.g., confirming the expression of the enzyme that acts on the quencher, and/or detecting a species conjugated to the enzyme.

摘要翻译： 一方面，本发明提供了荧光探针和测定法。 探针包括响应于猝灭剂的反应经历从暗变为荧光的荧光团 - 猝灭剂对。 探针从黑暗到荧光的切换通常由直接或间接作用于猝灭剂的酶介导，干扰其从荧光团中淬灭荧光发射的能力。 另一方面，本发明提供报道基因测定系统和使用该系统的方法。 测定系统包括荧光团猝灭剂探针和直接或间接作用于猝灭剂的酶，增加荧光团的荧光发射。 在其它方面，本发明提供了表达这些构建体的肽产物的核酸构建体和细胞。 在本发明的测定中，通过由猝灭剂的氧化态的变化介导的荧光的转换来检测目标物质的存在。 测定系统可用于许多测定形式，例如确认作用于猝灭剂的酶的表达和/或检测与酶缀合的物质。

公开(公告)号：US5147608A

公开(公告)日：1992-09-15

申请号：US188453

申请日：1988-04-29

申请人： Derek Hudson ,  Jordan Honig ,  Ronald M. Cook ,  Douglas J. Ng

发明人： Derek Hudson ,  Jordan Honig ,  Ronald M. Cook ,  Douglas J. Ng

IPC分类号： C07H21/04 ,  B01J19/00 ,  C07K1/04 ,  C40B40/10 ,  C40B40/12 ,  C40B60/14 ,  C40B70/00

CPC分类号： C07K1/045 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/0029 ,  B01J2219/005 ,  B01J2219/00549 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00686 ,  B01J2219/00725 ,  B01J2219/00731 ,  C40B40/10 ,  C40B40/12 ,  C40B60/14 ,  C40B70/00 ,  Y10T436/11

摘要： A chemical processing system is disclosed for the automated dissolution, dispersing and reaction of chemicals, especially for synthesizing proteins. The system includes a plurality of storage cartridges containing a first chemical in fluid communication with a reservoir containing a second chemical to be reacted with the first chemical. Each cartridge includes a pump (e.g., a plunger) which operates by changing the internal volume of the cartridge. The pump permits bi-directional flow of the first chemical into and out of the reservoir and cartridge to promote mixing and reacting with the second chemical in the reservoir to produce a third chemical.